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Inside A Cell

What do all cells have?


Ribosomes
DNA/RNA
Cell Membrane
Size: As cells size increases, they increase more in volume than area. Cells are small because it leaves more cell
membrane available for diffusion, which makes chemical reactions occur faster, so cell is more efficient.
Prokaryote vs. Eukaryote?
Eukaryotic
cells have individual
organelles other than
ribosomes, DNA, and
cell membrane and
more genetic
diversity, because
genes can more easily
be turned on and off.
They also have
different steps in
synthesizing proteins,
prokaryotic is much
more simple.
Eukaryotic cells
organize their DNA with chromosomes, and tend to have more DNA.

Click on https://learn.genetics.utah.edu/content/cells/insideacell/

Organelle Role of the organelle Plant or Animal Prokaryote or


Eukaryote
Lysosome and -useless proteins are taken to lysosome to be both Eukaryotic
Vesicles degraded. –Motor protein’s carry vesicles along
microtubules to their destination.
Mitochondria Once free living –components include: DNA, both eukaryotic
membrane, ribosomes. Cellular respiration-
powerhouse of cell
Cytoskeleton -serves as a road for motor protiens carrying both both
vesicles, gives the cell structure and helps organize
events of cell division.
Golgi Apparatus -vesicles with proteins/other macromolecules fuse both eukaryotic
with Golgi membrane and are tagged with specific
labels for transport proteins to deliver.
Nucleus -stores the cell’s DNA blueprint, keeping it from Both Eukaryote
contact with the activity of cytoplasm or
interference.
Endoplasmic -ribosomes attach to the rough ER where RNA is Both Eukaryote
Reticulum translated. Smooth ER stores enzymes and
chemical reactions occur here. Protein’s and fats go
from the ER to the Golgi through vesicles.
Cell Wall Protects Plant from injury and structural support Plant Eukaryotes

Chloroplast Once free living –components include: Plants, Algae, Some Eukaryotes
DNA/Membrane/Ribosomes Protista
Photosynthesis creates sugar
Vacuole -stores nutrients, breaking down waste, help cell both eukaryote
grow, and providing pressure needed for plant
growth. In animal cells vacuoles are much smaller
and store food/nutreints.
Cell membrane -semi-permeable lipid membrane which allows for both both
small molecules to pass through easily and large
molecules to need transport protein’s.
Nucleolus Helps assemble ribosomes and modify tRNA both eukaryote

DNA Stores genetic information—“blueprint” both both

Click on “Directing Traffic: How Vesicles Transport Cargo”


A vesicle forms when the membrane bulges out and pinches off. It travels to its destination then merges with another
membrane to release its cargo. In this way proteins and other large molecules are transported without ever having to cross
a membrane.
Watch the video --- “how vesicles travel cellular highways”. Complete the following.
Busy cells are often filled with thousands of traveling vesicles. To help organize these vesicles and get them pointed in the
right direction, the cell uses the rigid filaments and tubes of the cytoskeleton. Special motor protein’s attach to cargo-filled
vesicles and carry them along the cytoskeleton like trucks on a highway.
Motor proteins attach to vesicles and walk along a microtubule of the cytoskeleton. Dyneins walk toward the microtubule
organizing center (MTOC, or centrosome) and kinesins walk away from the MTOC.

What is the real-time video showing? Vesicles that carry green fluorescent protein and travelling along
the 3 neurons of the fruit fly embryo.

Click on “Membranes Organize Cellular Complexity”

Why are membranes so important for a cell?

They organize proteins and other molecules allowing to work in a much more efficient manner. Lysosome for example

holds in enzymes that would destroy other needed proteins if there was no membrane.
Membrane protein’s that contact the spaces on both sides of the membrane the gate-keepers of cellular compartments.

Each type of compartments has a specific population of membrane proteins that largely define its function. On average,

proteins make up about half the mass of membranes.

The nuclear pore complex (see scanning electron micrographs at right) is a unique protein structure that controls traffic
flow in and out of the nucleus.

Draw the “Nuclear pore complexes on the nuclear membranes of frog oocytes” from cytoplasm(left) from nucleus(right)

Making Proteins

Making, modifying, and delivering proteins to the right location is crucial for cells. Eukaryotic cells in
particular have what we call the endomembrane system for coordinating these activities. Make a flow chart to
discuss what structures are involved in making the following types of proteins.

free ribosome pathway – for making proteins destined to be free floating in the cytoplasm

DNA from nucleus

Transcribed into mRNA

Travels out of the nucleus membrane

Attaches to an RNA molecule

Translated into a free cytoplasmic protien

bound ribosome pathway – for making proteins destined to be or


_________________________
DNA from nucleus.

Transcribed into mRNA.

Travels out of nucleus membrane.

Attaches to an ribosome since it has a “tag”

Ribosome travels to the rough ER.

Protein is translated by ribosome and stored/modified a bit in the ER.


Vesicle with protein forms off of the ER and travels to the Golgi

In the Golgi the proteins are modified and “tagged”

Proteins are sent out into the cell membrane or other cells.

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