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    Uric Acid: (Uricase / Pod Method)

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    Dharmesh Patel
    This document provides information on a uric acid test kit, including: - Uric acid levels are clinically significant for conditions like gout and kidney dysfunction. - The kit uses uricase and peroxidase enzymes to convert uric acid to a colored product, allowing quantitative measurement of uric acid in samples. - Test procedure involves adding sample and reagents to test tubes, incubating, and measuring absorbance to determine uric acid concentration compared to a standard.

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    Download as PDF, TXT or read online from Scribd

    Uric Acid: (Uricase / Pod Method)

    Uploaded by

    Dharmesh Patel
    1K views1 page
    This document provides information on a uric acid test kit, including: - Uric acid levels are clinically significant for conditions like gout and kidney dysfunction. - The kit uses uricase and peroxidase enzymes to convert uric acid to a colored product, allowing quantitative measurement of uric acid in samples. - Test procedure involves adding sample and reagents to test tubes, incubating, and measuring absorbance to determine uric acid concentration compared to a standard.

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    Original Title

    URIC ACID

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    This document provides information on a uric acid test kit, including: - Uric acid levels are clinically significant for conditions like gout and kidney dysfunction. - The kit uses uricase and peroxidase enzymes to convert uric acid to a colored product, allowing quantitative measurement of uric acid in samples. - Test procedure involves adding sample and reagents to test tubes, incubating, and measuring absorbance to determine uric acid concentration compared to a standard.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    1K views1 page

    Uric Acid: (Uricase / Pod Method)

    Uploaded by

    Dharmesh Patel
    This document provides information on a uric acid test kit, including: - Uric acid levels are clinically significant for conditions like gout and kidney dysfunction. - The kit uses uricase and peroxidase enzymes to convert uric acid to a colored product, allowing quantitative measurement of uric acid in samples. - Test procedure involves adding sample and reagents to test tubes, incubating, and measuring absorbance to determine uric acid concentration compared to a standard.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    URIC ACID

    (URICASE / POD METHOD)


    INTENDED USE:
    This reagent kit is intended for the "in vitro" quantitative
    determination of Uric Acid in serum / plasma / Urine.
    Mix well and incubate at 37° C for 5 min. Measure the
    CLINICAL SIGNIFICANCE: absorbance of the Standard (Abs.S), and Test (Abs.T)
    Uric acid is the end product of the Purine metabolism. Uric acid is against the reagent blank at 505 nm.
    excreted to a large degree by the kidneys and to smaller degree in
    the intestinal tract by microbial degradation. Increased levels are CALCULATION :
    found in Gout, arthritis, impaired renal functions, and starvation.
    Decreased levels are found in wilson’s disease, Fanconis syndrome Abs. T
    and yellow atrophy of the liver. Uric acid (mg/dl.) = x6
    Abs. S
    PRINCIPLE: NORMAL VALUE :
    Uricase converts uric acid to allantoin and hydrogen peroxide. The Serum : Male : 4.0 - 7.2 mg /dl
    hydrogen peroxide formed further reacts with a phenolic compound Female : 2.7 - 6.5 mg/dl
    and 4 aminoantipyrine by the catalytic action of peroxidase to form a Urine : 250 - 750 mg/24 hrs
    red coloured quinoneimine dye complex. Intensity of the colour Each Laboratory should establish it's own normal
    formed is directly proprtional to the amount of uric acid present in the range representing its patient population.
    sample.
    LINEARITY :
    REACTION:
    Uricase This procedure is linear upto 20 mg/dl. If values exceed this
    Uric Acid + H2O Allantoin + H 2O2 limit, dilute the serum with normal saline (NaCl 0.9%) and
    repeat the assay.Multiply result by dilution factor.
    Peroxidase
    H2O 2+ 4 AAP + DHBS Red Quinoneimine + H2O QUALITY CONTROL :
    For accuracy it is necessary to run known controls
    with every assay.
    CONTENTS:
    Reagent 1 : Uric Acid Enzyme Reagent LIMITATION & PRECAUTIONS :
    Reagent 2 : Uric Acid Standard 6 mg/dl 1. Storage conditions as mentioned on the kit to be adhered.
    2.Do not freeze or expose the reagents to higher temperature
    MATERIALS REQUIRED BUT NOT PROVIDED: as it may affect the performance of the kit.
    - Clean & Dry Glassware. 3.Before the assay bring all the reagents to room
    - Laboratory Glass Pipettes or Micropipettes & Tips. temperature
    - Colorimeter or Bio-Chemistry Analyzer. 4. Incase of turbidity in urine, heat the sample for 10 minutes
    at 60° C to dissolve any possible Urate precipitates.
    SAMPLES: 5. Avoid contamination of the reagent during assay process.
    Unhemolysed Serum, Heparinized/EDTA plasma, Urine. Dilute urine 6. Use clean glassware free from dust or debris.
    (1:99) and multiply result by 100.
    BIBLIOGRAPHY :
    PREPARATION OF REAGENT & STABILITY : 1. Barham, D., and Trinder, P., Analyst 97, 142-145 (1972)
    All reagents are ready to use and are stable till the expiry date 2. Fossati, P., Presscipe, L., and Bertr, G., Ciln. Chem.
    mentioned on the label, when stored at 2 - 8 ° C.

    GENERAL SYSTEM PARAMETERS: CODE NO. PACK SIZE Reagent 1 Reagent 2


    Reaction type : End point S15C 5 x 20 ml 5 x 20 ml 3.0 ml
    Wave Iength : 505 nm. (490-530nm)
    S15D 4 x 50 ml 4 x 50 ml 3.0 ml
    Temperature : 37°C
    S15E 2 x 20 ml 2 x 20 ml 3.0 ml
    Incubation : 5 minutes
    Reagent volume : 1.0 ml.
    Sample volume : 25 µl. IVD
    Standard concentration : 6.0 mg/dl EC REP
    UNIONPROJET S.R.L.S.
    Via Aliprandi 41-20851 www.jas-anz.com.au/register

    Lissone (MB) ISO 9001:2015


    Zero setting : Reagent blank ISO 13485:2003

    Light path : 1 cm

    PROCEDURE :.
    Pipette into clean dry test tubes labeled as Blank (B), Standard
    (S) and Test (T) :

    Addition Sequence B. S. T.
    Enzyme reagent 1 ml 1 ml 1 ml
    Standard - 25 µl -
    Sample - - 25 µl

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