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    EunicePatriciaM.Villanueva
    The document presents a conceptual framework for an experiment to test the quorum sensing inhibition properties of silver nanostructures synthesized using green methods against Pseudomonas aeruginosa. The experiment will involve green synthesis of silver nanostructures, characterization of their size and composition, and assessment of their ability to disrupt quorum sensing in P. aeruginosa using a bioassay with Chromobacterium violaceum. Disruption of quorum sensing will be indicated by loss of pigmentation in the C. violaceum.

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    EunicePatriciaM.Villanueva
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    The document presents a conceptual framework for an experiment to test the quorum sensing inhibition properties of silver nanostructures synthesized using green methods against Pseudomonas aeruginosa. The experiment will involve green synthesis of silver nanostructures, characterization of their size and composition, and assessment of their ability to disrupt quorum sensing in P. aeruginosa using a bioassay with Chromobacterium violaceum. Disruption of quorum sensing will be indicated by loss of pigmentation in the C. violaceum.

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    The document presents a conceptual framework for an experiment to test the quorum sensing inhibition properties of silver nanostructures synthesized using green methods against Pseudomonas aeruginosa. The experiment will involve green synthesis of silver nanostructures, characterization of their size and composition, and assessment of their ability to disrupt quorum sensing in P. aeruginosa using a bioassay with Chromobacterium violaceum. Disruption of quorum sensing will be indicated by loss of pigmentation in the C. violaceum.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    17 views1 page

    Dfsfs

    Uploaded by

    EunicePatriciaM.Villanueva
    The document presents a conceptual framework for an experiment to test the quorum sensing inhibition properties of silver nanostructures synthesized using green methods against Pseudomonas aeruginosa. The experiment will involve green synthesis of silver nanostructures, characterization of their size and composition, and assessment of their ability to disrupt quorum sensing in P. aeruginosa using a bioassay with Chromobacterium violaceum. Disruption of quorum sensing will be indicated by loss of pigmentation in the C. violaceum.

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    CONCEPTUAL FRAMEWORK

    - Pseudomonas - Experiment in -Disruption of the

    PROCESS

    OUTPUT
    INPUT

    Aeruginosa making a quorum sensing


    - Green nanostructure of the bacteria
    synthesized Silver - Checking with the use of
    nanostructures, nanostructure sizes, different silver
    particularly elements, and nanostructures
    nanowires, contents inside a
    nanoparticles and molecule
    nanoprism -Making a set up
    for the quorum
    sensing

    The paradigm of the study shows how will the researcher conduct the said experiment about the quorum
    sensing inhibition properties of green synthesized silver (au) nanostructures against pseudomonas
    aeruginosa. The flow of the experimental research will be according to the chart which is in I-P-O format.

    The input of the study shows the variable affected in the experiment, while in the part of process shows
    how will the researcher will conduct the said experiment, which will start with the use of green synthesis.
    Green synthesis uses plants to make nanostructures, the plant that will be used in this experiment is a
    basil. The nanostructures sizes will be checked by a Scanning Electron Microscope (SEM), moreover the
    elements inside the nanostructure will be checked by using Energy-Dispersive X-ray (EDX), lastly the
    contents inside the molecules, organic compound will be checked by an Ultra Violet - Visible Spectroscopy
    (UV - vis).

    In making the set up of the quorum sensing, a bacteria called chromobacterium violaceum will be used in
    this process. Then an Agar Plate will grow and will be covered by a pseudomonas aeruginosa. Then, the
    silvers of the nanostructures will be placed on round or discs flatforms.

    After placing the nanostructures the chromobacterium violaceum, which is in the color violet, will lose its
    pigment indicating that the quorum sensing is disrupted. The chromobacterium violaceum produces
    molecules that binds the autoinducer, which is used to do a quorum sensing. Therefore if the quorum
    sensing is disrupted then it wont bind the components, which will result in creating a discoloration. And
    lastly the output shows the outcome of the experiment.

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