ABX Pentra DX120

Hematology analyzer

45 parameters
Integrated slide-maker
Expert validation system
 ABX Pentra DX 120
In the heart of hematopoiesis

Complete cytology platform

> 120 samples per hour
Continuous loading option
150-tube loader capacity
> 45 parameters
CBC (12), DIFF (20), RET (10), NRBC (3)
> Automatic reflex testing
Immediate confirmation of tests based on the chosen
validation rules
> Automatic validation of results
Laboratory-defined rules
> Integrated cytology atlas
Hematovision, a software designed and developed by HORIBA Medical
> SPS evolution
Integrated smearer stainer (optional)
 < Hematopoiesis through ABX Pentra DX 120
Scientific book

Differentiation and quantification of hematopoietic

populations using 7 analytical systems
> Erythropoiesis
3 dedicated channels: erythroblasts / reticulocytes / erythrocytes
> Thrombopoiesis
2 dedicated channels: thrombocytes / double matrix
> Leukopoiesis
4 dedicated channels: leukocytes / double matrix / basophils / erythroblasts

DHSS Concept
Flow cytometry
Injection of the sample prepared in a double
hydrodynamic flow cytometer (HORIBA Medical
patent), and determination of the cell complexity
by measuring the absorbance of a polychromatic
light source, or the fluorescence by
fluorocytometry (with argon-ion laser).

Cytochemistry
Incubation of the sample at a regulated
temperature and cells stained with Chlorazole
Black. This reagent stains specifically leukocyte
cytoplasm, granules, and nuclei.
 Granulocytic line

Monocytic line
Double DIFF matrix
Eosinophil
Full leukopoiesis analysis:
Routine identification and quantification of three immature cell lines
Monocyte - granulocytic line (IMG)
- lymphocytic line (IML)
Neutrophil - monocytic line (IMM)

Reduction of the number of slide examinations

Diagnostic and follow-up tool for rapid decision-making

Lymphocytic line

Lymphocyte

Erythroblasts
Fluorescence-based count: WBC population

Erythroblast analysis in routine or reflex mode based on:

- internal laboratory rules
- detection alarms
- patient demographics...

Use of Thiazole Orange fluorochrome

Automatic correction of leukocyte count (WBC Fluo)

Erythroblast
Double Hydrodynamic Sequential System (HORIBA Medical Patent)

Reticulocytes
Differential diagnosis of anemia:
Simultaneous count of 10 parameters
Classification according to three stages
of maturation (Heilmeyer):
- RET High
Reticulocyte population - RET Medium
- RET Low

Follow-up of iron-deficiency anemia based on the

MRV (Mean Reticulocyte Volume)

t on
tion
tio
RBC population
Confirmation of bone marrow regeneration level
based on the CRC (Corrected Reticulocyte Count)

A concentrate of technology
5 recognised measurement principles included on a single analyzer.
1

Reference methods CBC DIFF RET NRBC

Impedancemetry • • • •
2
Flow cytometry • • •
4
Fluorometry • • 1. Argon Laser
2. Photomultiplicator
Cytochemistry • • 3. Flow cytometer
3 4. Impedancemetry
DHSS • • • 5. Optical measurement
5
SPS Evolution
Perfect standardization of blood smears

• Integrated slide-maker*
120 slides per hour
Fully automated and secured process
• Choice of staining protocols
May-Grünwald Giemsa, Wright, Wright Giemsa, etc.
• Sampling volume: 50 µl of whole blood (added)
On primary closed tube
• Positive identification
Barcode reading
Patient data printed automatically on the slide
• Smearing flexibility
User-defined smearing profiles based on the laboratory’s own rules

* optional module

Validation station
Result validation

• Full management of samples

Alarms triggered according to patient profile
Delta check
Programmable rerun rules
• Manual entry capability
Results, comments, etc.
• Automatic validation
Based on rules, flags, patient history, etc.
• Integrated cytology atlas
Hematovision
 Subject to technical modifications / HAN716BEN / © 2009 HORIBA Medical - HORIBA ABX SAS - France - RCS Montpellier 328 031 042
ABX Pentra DX 120

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