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    Direct HDL-Cholesterol Kit (D-HDL-C) : Cat. 0200 Clearance Method Size: R1 (60ml×1) + R2 (20ml×1)

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    Fauzan Hashifah Permana
    1. This document provides instructions for using a direct HDL-cholesterol kit to quantify HDL-cholesterol levels in human serum. 2. The kit uses cholesterol esterase, cholesterol oxidase, and catalase in Reagent 1 to eliminate non-HDL cholesterol, followed by a specific reaction of HDL-cholesterol with detergent in Reagent 2. 3. Expected HDL-cholesterol levels for adults are 1.16-1.42 mmol/L for males and 1.29-1.55 mmol/L for females. The test is linear up to 2.59 mmol/L.

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    Direct HDL-Cholesterol Kit (D-HDL-C) : Cat. 0200 Clearance Method Size: R1 (60ml×1) + R2 (20ml×1)

    Uploaded by

    Fauzan Hashifah Permana
    48 views3 pages
    1. This document provides instructions for using a direct HDL-cholesterol kit to quantify HDL-cholesterol levels in human serum. 2. The kit uses cholesterol esterase, cholesterol oxidase, and catalase in Reagent 1 to eliminate non-HDL cholesterol, followed by a specific reaction of HDL-cholesterol with detergent in Reagent 2. 3. Expected HDL-cholesterol levels for adults are 1.16-1.42 mmol/L for males and 1.29-1.55 mmol/L for females. The test is linear up to 2.59 mmol/L.

    Original Description:

    insert kit HDL cholesterol

    Original Title

    D-HDL

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    1. This document provides instructions for using a direct HDL-cholesterol kit to quantify HDL-cholesterol levels in human serum. 2. The kit uses cholesterol esterase, cholesterol oxidase, and catalase in Reagent 1 to eliminate non-HDL cholesterol, followed by a specific reaction of HDL-cholesterol with detergent in Reagent 2. 3. Expected HDL-cholesterol levels for adults are 1.16-1.42 mmol/L for males and 1.29-1.55 mmol/L for females. The test is linear up to 2.59 mmol/L.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    48 views3 pages

    Direct HDL-Cholesterol Kit (D-HDL-C) : Cat. 0200 Clearance Method Size: R1 (60ml×1) + R2 (20ml×1)

    Uploaded by

    Fauzan Hashifah Permana
    1. This document provides instructions for using a direct HDL-cholesterol kit to quantify HDL-cholesterol levels in human serum. 2. The kit uses cholesterol esterase, cholesterol oxidase, and catalase in Reagent 1 to eliminate non-HDL cholesterol, followed by a specific reaction of HDL-cholesterol with detergent in Reagent 2. 3. Expected HDL-cholesterol levels for adults are 1.16-1.42 mmol/L for males and 1.29-1.55 mmol/L for females. The test is linear up to 2.59 mmol/L.

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    Direct HDL-Cholesterol Kit (D-HDL-C)

    Cat. 0200
    Clearance method
    Size: R1 (60ml×1) + R2 (20ml×1)
    Intended Use: Blank is calibrated to zero.
    For the quantitative determination of high-density lipoprotein Read Acalibrator and Asample.
    cholesterol (HDL-C) in human Serum

    Principle of reaction: Calculation：

    1. Elimination of chylomicron (CM), LDL-cholesterol and Acalibrator


    VLDL-cholesterol by cholesterol esterase, cholesterol
    oxidase and catalase in Reagent 1. This is colorless reaction. Con. of HDL-C＝ -------- ×Con. of Calibrator
    2. Specific reaction of HDL-cholesterol after release of HDL-C Asample (mmol/L or mg/dL)
    by detergent in Reagent 2.
    Components: Expected Values:
    Reagent Content Concentration Adult Males: 1.16-1.42mmol/L (45-55mg/dL)
    Cholesterol esterase
    ≥1000U/L Adult Females: 1.29-1.55mmol/L (50-60mg/dL)
    Cholesterol oxidase
    R1 ≥800U/L Regulation:＞1.04mmol/L (＞40mg/dL)
    Catalase
    ≥900U/L
    Low value:＜0.91mmol/L
    Toos

    4AA Conversions:

    detergent mmol/L×38.67=mg/dL; mg/dL×0.02586=mmol/L


    R2
    Peroxidase (POD) The reference range should be determined by each hospital to
    ≥4000U/L
    confirm with the characteristics of the region being tested.
    NaN3
    Linearity：
    D-HDL-C calibrator serum(note on the label)
    The test is linear up to 2.59mmol/L(100mg/dL)
    Shelf Life:
    12 months at 2～8℃, protecting from light.
    Precautions：
    Sample:
    1. If working solution show purple color, discard the reagents.
    Fresh Serum ， or EDTA and heparinized plasma on an empty 2. In vitro use only. Sodium azide is include, avoid ingestion or
    stomach are the recommended specimens. Centrifuge and contact with skin or mucous membranes.
    remove the serum or plasma as soon as possible after collection
    (within 3 hours). 3. Human material used in formulating this calibrator has been tested
    and found to be negative for HBs antigens, anti-HCV antibody, and
    The sample is stable in 12 hours at room temperature(15~30℃), anti-HIV antibody. However, handle with care as patient sample
    5 days at 2~8℃, or 1 month long when kept frozen in -20 ℃ or preparation is recommended.
    below. Once frozen or melted, it shall not be refrozen. Extreme
    turbidity serum should be diluted before assaying. 4. Shall be stored at 2 ～ 8℃. Avoid being stored at temperature
    over 8℃ or below 0℃.
    Assay conditions:
    Wavelength：546nm Bibliography：
    Temperature：37℃
    Cuvette：1cm. 1. 《Expected Values Handbook of Laboratory Medicine》, Li-hua Zhu
    Procedure:
    1998, (in Chinese)
    Ready to use.

    Blank Calibrator Sample


    Registration number：SFDA-3050260(2003)
    Sample -- -- 6μl Standard number：YZB/GB 0667-2003
    Calibrator -- 6μl --

    Water 6μl -- -- Biosino Bio-Technology and Science Inc.


    R1 600μl 600μl 600μl Add：No.27, Chaoqian Road, Changping District,
    102200 Beijing, China
    Incubate 5 minutes at 37℃
    Tel：+86-10-69722840, Fax：+86-10-69722840
    R2 200μl 200μl 200μl Website：www.zhongsheng.com.cn
    Email: trade@zhongsheng.com.cn
    Incubate 5 minutes at 37℃

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