1 MCQ Biochemistry For - Paramedical - Students PDF
1 MCQ Biochemistry For - Paramedical - Students PDF
1 MCQ Biochemistry For - Paramedical - Students PDF
MCQs
•
in
Biochemistry
for
Paramedical Students
P Ramamoorthy
PhD (Biochemistry-Faculty of Medicine)
Professor and Head
Department of Biochemistry
Mahatma Gandhi Medical College and Research Institute
Pondicherry University, Pondicherry
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I am most grateful to the management of the Mahatma
MCQs in Biochemistry for Paramedical Students
Gandhi Medical College and Research Institute, Pondicherry
© 2006, P Ramamoorthy University, Pondicherry for bringing out the book in time. I
am thankful to M/s Jaypee Brothers Medical Publishers (P)
All rights reserved . No part of this publication should be reproduced, stored in a retrieval system,
or transmitted in any form or by any means: electronic, mechanical, photocopying, recording, Ltd. for publication of the book with fine features.
or otherwise, without the prior written permission of the author and the publisher.
This book has been published in good faith and belief that the material provided by author
is original. Every effort is made to ensure accuracy of material, but the publisher, printer and
author will not be held responsible for any inadvertent error(s). In case of any dispute, all legal P Ramamoorthy
matters to be settled under Delhi jurisdiction only.
ISBN 81 -8061-701-7
Contents
'-'
1 B 2 A 3 C 4 D 5 A
2 MCQs in Biochemistry for Paramedical Students The Cell 3
18 C 19 B 20 A
1 D 2 c 3 A 4 B 5 A 6 D
6 MCQs in Biochemistry for Paramedical Students Chemistry of Carbohydrates 7
7. Blood sugar refers to:
15. Mutarotation by glucose arises due to:
A. Fructose B. Glucose A. Opening of hemiacetal ring
C. Glycogen D. Lactose B. Formation of glycosides
8. Dextrose is: C. Inversion of sugar
A. D( +)Glucose D. Conversion of keto-enol forms
B. D{-)Glucose
C. D( +)Fructose D. D(-)Fructose 16. Glucose on oxidation with H 20 2 gives:
9. Stereoisomers differ from each other in: A. Hyaluronic acid
A. Atomic weights B. Glucuronic acid
B. Atomic numbers C. Glucaric acid
C. Spatial configuration D. Mucic acid
D. All of the above
17. Sorbitol is:
10. All the monosaccharides are optically active except: A. A sterol
A. Glyceraldehyde B. An amino alcohol
B. Fructose C. A sugar alcohol
C. Arabinose D. A glycerol derivative
D. Dihydroxyacetone 18. The functional group responsible for the reducing
11. Number of asymmetric carbon atoms present in the property of glucose is present on which carbon atom?
straight chain structure of D-Glucose is: A.6 B. 5
A. 6 B. 4 C. 2 D. 1
C. 3 D. 2 19. Aminosugar containing drug is:
12. Ring (cyclic) structures for sugars were proposed by: A. Erythromycin
A. Fischer B. Watson and Crick B. Streptomycin
C. Haworth D. Sanger C. Ouabain
D. None of the above
13. a-D-glucopyranose and P-D-glucopyranose are related
by: 20. Which one of the following is a glycoside?
A. Keto-aldo pair A. Triglyceride
B. Keto-enol pair B. Glutathione
C. Epimers C. Digitonin
D. Anomers D. All of the above
14. Glucose and galactose are epimers which differ in 21. Aglycone is a component of:
orientation of H and OH on: A. Glycogen B. Starch
A. C6 B. C4 C. Glycoside D. Mucopolysaccharide
C. C2 D.C1
7 B 8 A 9 C 10 D 11 B 12 C 13 D 15 A 16 B 17 C 18 D 19 A 20 C 21 C
14 B
8 MCQs in Biochemistry for Paramedical Students Chemistry of Carbohydrates 9
22. Which of the following sugars is found in RNA? 30. All the following are reducing sugars except:
A. Ribose B. 2-Deoxy-D-ribose A. Maltose B. Lactose
C. Fructose D. Ribulose C. Sucrose D. Fructose
23. Inverted sugar is: 31. Which polysaccharide (glycan) is not a polymer of
A. Fructose glucose?
B. Glucose A. Glycogen B. Starch
C. Sucrose C. Cellulose D. Inulin
D. A mixture of glucose and fructose
32. All the following are homopolysaccharides (homogly-
24. All the following are disaccharides except: cans) except:
A. Sucrose B. Maltose A. Heparin
C. Raffinose D. Lactose B. lnulin
25. Lactose contains: C. Glycogen
A. Glucose and glucose B. Glucose and galactose D. Starch
C. Glucose and fructose D. Galactose and fructose 33. The branched component of starch is:
26. Sucrose is known as: A. Amylose
A. Table sugar B. Milk sugar B. Amylopectin
C. Malt sugar C. Dextrin
D. Fruit sugar
D. Amylase
27. Which of the following statements is correct?
A. Cellulose is a heteropolysaccharide 34. Dextrins are polysaccharides formed as intermediate
B. Glycogen is the storage form of glucose in human compounds during hydrolysis of:
C. Sucrose can form osazone A. Glycogen
D. Oligosaccharides contain 5-10 saccharide units. B. Dextran
C. Starch
28. Which of the following statements is not correct? D. Cellulose
A. Seminal fluid is rich in glucose
B. Inulin is a fructosan 35. Glycogen contains:
C. Molisch's test is used to identify carbohydrates A. a. 1,4 and a. 1,6 glycosidic linkages
D. Sialic acid is a constituent of glycoproteins B. a. 1,6 glycosidic linkages
C. a. 1,4 and ~ 1,6 glycosidic linkages
29. What is the shape of maltosazone crystals? D. ~ 1,4 and ~ 1,6 glycosidic linkages
A. Needle/bundle of hay
B. Rhombic plates 36. The polysaccharide used to determine glomerular
C. Hedgehog/powder puff/cotton ball
filtration rate (GFR) is:
D. Sunflower petals A. Starch B. Inulin
C. Heparin D. Dex tr an
22 A 23 D 24 C 25 B 26 A 27 B 28 A 30 C 31 D 32 A 33 B 34 C 35 A 36 B
29 D
1O MCQs in Biochemistry for Paramedical Students
6. The major storage lipid in adipose tissue is: l4. Which compounds facilitate emulsification of fats?
A. Phospholipids B. Cholesterol A. Bile salts
C. Fatty acids D. Triglycerides B. Bile pigments
C. Bile acids
7. Which substance yields more calories per gram on
oxidation? D. Sterols
A. Fats B. Carbohydrates 15. Hydrolysis of fat by alkali is known as:
C. Plant proteins D. Animal proteins A. Esterification
8. Neutral fats are:
B. Saponification
C. Emulsification
A. Glycolipids B. Phospholipids
C. Triglycerides D. Peroxidation
D. Waxes
16. Iodine number indicates:
9. Rancidity can be prevented by antioxidants like:
A. Total number of fatty acids in fat
A. Riboflavin B. Vitamin E
C. Vitamin D B. Level of rancidity of fat
D. Vitamin B6 C. Measure of the degree of unsaturation in fat
10. Which am_
o ng the following is a saturated fatty acid?: D. Number of volatile fatty acids in fat
A. Stearic acid B. Oleic acid
17. Acrolein test is performed to detect:
C. Linoleic acid D. Aspartic acid A. Chloesterol B. a-amino acids
11. All the following are polyunsaturated fatty acids C. Triglycerides D. Glycerol
(PUFA) except:
18. The main lipid constituents of cell membrane are:
A. Linolenic acid
A. Phospholipids
B. Palmitic acid
B. Cholesterol
C. Arachidonic acid
C. Triacylglycerols
D. Linoleic acid
D. Glycolipids
12. Chaulmoogric acid, a cyclic fatty acid was used to treat: 19. Cardiolipin, a phospholipid is present in:
A. Bronchitis
A. Nucleus
B. Bone disorders
B. Mitochondrial membrane
C. Cancer
C. Ribosomes
D. Leprosy
D. Endoplasmic reticulum
13. Which of the following is not an essential fatty acid 20. Lecithins contain:
(EFA)?
A. Glycerol, fatty acid, phophoric acid, choline
A. Linoleic acid
B. Glycerol, fatty acid, phosphoric acid, serine
B. Linolenic acid
C. Glycerol, fatty acid, phosphoric acid, ethanolamine
C. Oleic acid
D. Sphingosine, fatty acid, galactose
D . Arachidonic acid
6 D 7 A 8 c 9 B 10 A 11 B 12 D 14 A 15 B 16 C 17 D 18 A 19 B 20 A
13 c
14 MCQs in Biochemistry for Paramedical Students Chemistry of Lipids 15
21. Respiratory distress syndrome occurs due to deficiency Lipoproteins can be separated into different fractions
27.
of: by:
A. Plasmalogen A. Electrophoresis
B. Spingomyelin B. Ultracentrifugation
C. Dipalrnitoyl lecithin c. Both of the above
D. Phosphatidyl serine D. Chromatography
22. Which is a major constituent of lung surfactant? 28. Which of the following fractions of lipoproteins has
A. Dipalrnitoyl lecithin the lowest density?
B. Cholesterol A. Chylomicrons
C. Phosphatidic acid B. a-lipoproteins
D. Glycerol C. P-lipoproteins
D . Pre P-lipoproteins
23. All the following are examples of cerebrosides except:
A. Kerasin 29. Which fraction of lipoproteins has highest cholesterol
B. Cerebron content?
C. Nervon A. HDL B. Chlornicrons
D. Dextran C. LDL D.VLDL
30. The apolipoprotein B-100 is the major apolipoprotein
24. Carbohydrate moiety present in cerebroside is:
A. Fructose present in:
A. IDL B. VLDL
B. Galactose
C. HDL D. LDL
C. Ribose
D. Mannose 31. Cholesterol contains:
A. Corrin ring
25. Sphingosine, an amino alcohol is a constituent of all B. Pyrimidine ring
the following except: C. Cyclopentanoperhydrophenanthrene ring
A. Ganglioside D. Isoalloxane ring
B. Cerebroside
C. Cephalin 32. Which one of the following is an animal sterol?
D. Sphingomyelin A. Sitosterol B. Cholesterol
C. Ergosterol D. Stigmasterol
26. Which is not a constituent of gangliosides?
A. Sialic acid 33. Cholesterol crystals have the following shape when
B. Glycerol observed under microscope:
A. Needles
C. Sphingosine
B. Concentric layers
D. Long chain fatty acid
C. Hedgehog or cotton ball
D · Rhombic plates with notched corners
21 C 22 A 23 D 24 B 25 C 26 B 27 C 28 A 29 C 30 D 31 C 32 B 33 D
16 MCQs in Biochemistry for Paramedical Students
34. All- the following compounds are formed from
cholesterol except:
-
A. Bile pigments B. Vitamin D
C. Bile acids D. Steroid hormones
35. All the following are eicosanoids except:
A. Prostaglandins B. Glutathione Chemistry of Proteins
C. Thromboxanes D. Leukotrienes
36. Prostaglandins are synthesised from:
A. Palmitic acid B. Linoleic acid 1. Which of the following statements about amino acids
C. Stearic acid D. Arachidonic acid is not true?
37. Which one is not the function of prostaglandins? A. Amino acids are ampholytes
A. Induction of labour B. Amino acids are linked through peptide bonds to form
B. Prevention of myocardial infarction proteins
C. Lowering of blood pressure C. Amino acids are not crystalline compounds
D. Antiinflammatory D . Leucine is a purely ketogenic amino acid
38. Spingomyelins are increased in which of the following 2. Number of amino acids present in proteins are:
inherited disorders? A. 10 B. 20
A. Von Gierke's disease C. 100 D. 300
B. Gaucher's disease
3. The amino acids found in biological proteins are of:
C. Niemann-Pick disease
A. D-Configuration and dextrorotatory
D. Tay-Sachs disease
B. L-Configuration and laevoratatory
39. The lipid accumulating in Gaucher's disease is: C. D-Configuration and laevo or dextrorotatory
A. Glucosylceramide D. L-Configuration and dextro or laevoratatory
B. Lecithins
4. Which amino acid does not occur in proteins of
C. Cholesterol
biological system?
D. Galactosylceramide A. Ornithine
40. Liebermann-Burchard reaction is used to detect: B. Arginine
A. Vitamin A C. Cystine
B. Cholesterol D. Histidine
C. Glycerol
S. All amino acids are optically active except:
D. a-Amino acids A. Serine B. Glycine
C. Tryptophan D. Threonine
34 A 35 8 36 D 37 8 38 C 39 A 40 8 1 c 2 8 3 D 4 A 5 8
18 MCQs in Biochemistry for Paramedical Students Chemistry of Proteins 19
6. Which one of the following is an acidic amino acid? . All the following are essential amino acids except:
14
A. Palmitic acid B. Aspartic acid A. Phenylalanine B. Tryptophan
C. Pyruvic acid D. Lysine c. Tyrosine D. Isoleucine
7. A basic amino acid is: l 5. The semiessential amino acids are:
A. Phenylalanine B. Serine A. Histidine and alanine
C. Arginine D. Glutamic acid B. Arginine and glycine
8. An example for neutral amino acid is: C. Praline and methionine
A. Tyrosine B. Proline D. Arginine and histidine
C. Lysine D. Leucine 16. Which amino acid contains a guanidine group?
9. Which one of the following is not a sulphur containing A. Glutamine B. Asparagine
amino acid? C. Histidine D. Arginine
A. Histidine B. Cystine 17. An amino acid which behaves both as glucogenic and
C. Cysteine D. Methionine ketogenic is:
10. Branched chain amino acids are: A. Alanine
A. Tyrosine, tryptophan and phenylalanine B. Phenylalanine
B. Glycine, serine and threonine C. Leucine
C. Valine, leucine and isoleucine D. Cystine
D. Cystine, cysteine and methoinine 18. The last used to detect a amino acids is:
11. Phenylalanine, tyrosine and tryptophan are called as: A. Biuret test
A. Aliphatic amino acids B. Murexide test
C. Ninhydrin test
B. Aromatic amino acids
C. Sulphur containing amino acids D. Molisch's test
D. Imino acids 19. Which amino acid is important in the buffering action
12. Which amino acid contains an imino group? of proteins at physiological pH?
A. Glutamic acid
A. Histidine
B. Lysine
B. Glutamic acid
C. Histidine
C. Arginine
D. Tyrosine
D. Proline
20. Edman's reagent (Phenyl isothiocyanate) reacts with:
13. H 2N-CH2.COOH is the structure of:
A. N-terminal amino acids
A. Glycine B. Serine
B. C-terminal amino acids
C. Leucine D. Threonine
C. Unsaturated fatty acids
D . None of the above
6 B 7 c 8 D 9 A 10 C 11 B 12 D 14 C 15 D 16 D 17 B 18 C 19 C 20 A
13 A
20 MCQs in Biochemistry for Paramedical Students Chemistry of Proteins 21
21. Sorensen's formal titration method is used to estimate: 28 . Which is n ot ~econdary protein structure?
A. Free amino group in amino acid mixture A. Random coil
B. Free carboxyl group in amino acid mixture B. p-pleated sheet
C. Sulphur content of amino acids c. Double helix
D. Acidity in gastric juice o. a helix
22. A method to separate amino acids is: 29. At isoelectric pH, the protein molecule has:
A. Filtration A. Positive charge only
B. Electrophoresis B. Maximum positive charge than nagative
C. Chromatography C. More negative charge than positive
D. Dialysis D. Equal number of positive and negative charges
23. Proteins are: 30. The phosphoprotein present in egg yolk is:
A. Products of genes A. Ovovitellin
B. Polymers of amino acids B. Ovoglobulin
C. Colloids C. Ovalbumin
D. All of the above D. Casein
24. Albumin and globulins are: 31. Apoprotein is protein moiety of:
A. Simple proteins A. Mucoproteins
B. Conjugated proteins B. Lipoproteins
C. Primary derived proteins C. Chromoproteins
D. Secondary derived proteins D. Nucleoproteins
32. The most abundant protein present in the human body
25. All the following are conjugated proteins except: is:
A. Metalloproteins
A. Albumin B. Myosin
B. Hemoproteins
C. Collagen D. a Keratin
C. Histones
D. Lipoproteins 33. Which one of the following is a fibrous protein?
A. Collagen
26. An example for derived protein is:
B. Myoglobin
A. Globulin
C. Hemoglobin
B. Gelatin
C. Casein
D. None of the above
D. Protamine 34. Globular p rotein is:
27. Glutathione is a: A. Myoglobin B. Glutathione
A. Tripeptide C. Collagen D. a-keratin
B. Dipeptide
C. Protein D. Derivative of glucose
28 C 29 D 30 A 31 B 32 C 33 A 34 D
21 B 22 C 23 D 24 A 25 C 26 B 27 A
22 MCQs in Biochemistry for Paramedical Students
35 A 36 B 37 A 38 D 39 B 40 C 18 2 C 3A 4 D 5C
--
24 MCQs in Biochemistry for Paramedical Students Chemistry of Nucleic Acids 25
6. Which carbon of the pentose is in ester linkage with Double helical structure of DNA was proposed by:
the phosphate in a nucleotide? 13.
A. Chargaff
A. Cs B. C4 B. Watson and Crick
C. C3 D.C1 c. Hargobind Khorana
7. Which form of DNA is usually found under o. Stahl and Messelson
physiological conditions? l4. Two strands of double helical DNA are linked by:
A. A-DNA B. E-DNA A. Peptide bonds
C. Z-DNA D. B-DNA B. Phosphodiester bonds
8. 2-Deoxy-D-ribose is a constituent of: C. Glycosidic bonds
A.RNA B. mllim o. Hydrogen bonds
C. DNA D. Dextrin 15. ONA molecule contains how many nucleotides?
9. The base present in DNA but absent in RNA is: A. 100-300
A. Guanine B. Thymine B. 500-1000
C. Uracil D. Cytosine c. 1000-9000
o. 10000-15000
10. Which of the following bases is a constituent of RNA
but not DNA? 16. Chargaff' s rule states:
A. Thymme A. (A+G)=(T +C)
B. Adenine B. A/T=G/C
C. Cytosine C. C+C=A+C
D. Uracil D. A+T+G+C
11. Nucleotides in a single strand are linked to one another 17. DNA can be denatured by:
in nucleic acids by: A.Acid
A. Hydrogen bond B. Alkali
B. Glycosidic linkage C. Heat
C. Phosphodiester bond D. All of the above
D. Disulphide bond 18. Molecule of genetic information is:
12. Which compound has double helical structure? A. Protein B. DNA
A. Ribonucleic acid C. RNA D. Enzyme
B. Collagen 19. Gene is:
C. Deoxyribonucleic acid A.mRNA
D. Cholesterol B. Anticodon
C. A segment of DNA molecllie
D. A complete DNA molecllie
6 A 7 D 8 C 9 B 10 D 11 C 12 C 13 B 14 D 15 C 16 A 17 D 18 B 19 C
26 MCQs in Biochemistry for Paramedical Students Chemistry of Nucleic Acids 27
~~~~~~~~~~~~~~2-4----:-A~~2~5:--:
22 D 23 C
C~~2=s:--:::D:---~ 27 ~C;;--:-28~D~~29~B~-3-0~A~~~~~~~~~~~~
20 D 21 B
--
6·
Chemistry of Hemoglobin 29
14. In
A.
B.
C.
methemoglobin the iron is in:
Ferric form
Ferrous form
Ferric and ferrous forms
-- ---
21. A. HbA2
c. HbD
Chemistry of Hemoglobin
14 A 15 B 16 A 17 C 18 D 19 B 20 C 21 A 22 C 23 A 24 A 25 C
--
6.
.An exopeptidase is:
.A. Elastase
B. Chymotrypsin
Enzymes 33
c. Trypsin
0 . Carboxy peptidase
Enzymes 7
. .An example for an extracellular enzymes is:
.A. Glucokinase
B. Pancreatic amylase
1. Enzymes are:
c. Hexokinase
A. Biocatalysts
o. Glucose 6-phosphatase
B. Proteins except ribozymes s. .All the following are coenzymes except:
C. Products of genes .A. NAO+
D. All of the above B. TPP
C. SGPT (ALT)
2. According to IUB nomenclature system, enzymes are D. Pyridoxal phosphate
grouped in how many main classes?
A. 10 B. 6 9. The function of a coenzyme in an enzymatic reaction is
C.4 D.2 to:
A. Act as cosubstrate
3. Lactate dehydrogenase belongs to which main class of B. Raise the activation energy of the enzymatic reaction
enzymes? C. Enhance the specificity of the apoenzyrne
A. Ligases D. Activate the substrate
B. Lyases
C. Oxidoreductases 10. A cofactor in an enzymatic reaction is:
D. Isomerases A. An organic molecule
B. A metal ion
4. The enzymes present in lysosomes are:
C. Both of the above
A. Transferases
D. A hormone
B. Isomerases
C. Hydrolases 11. Action of urease on urea belongs to which type of
D. Lyases enzyme specificity?
A. Optical specificity
5. All the following gastrointestinal enzymes are secreted
B. Substrate specificity
as zymogens except:
C. Reaction specificity
A. Ribonucleases
D. Bond specificity
B. Pepsin
C. Chymotrypsin
D. Trypsin
6 D 7 B 8 C 9 A 10 C 11 B
1 D 2 B 3 c 4 c 5 A
34 MCQs in Biochemistry for Paramedical Students
13. Cobamide coenzymes are coenzymes of: Trypsin shows optimum activity at:
A. Vitamin B1 B. Biotin 20
· A. pH 1.5 B. pH 4.9
C. Vitamin B12 D. Vitamin C c. pH 6.9 D. pH 8.9
14. Coenzyme required for transamination is: . Lock and key model of mechanism of enzyme action
A. NAO+ 21
was proposed by:
B. Thiamin pyrophosphate A. Koshland
C. FMN B. Chargaff
D. Pyridoxal phosphate C. Fischer
15. Xanthine oxidase contains which metal ion? D. Louis Pasteur
A. Manganese 22. Which type of inhibition is irreversible?
B. Molybdenum A. Non-competitive
C. Copper B. Uncompetitive
D. Cobalt C. Competitive
D. None of these
16. Which of the following factors that can affect enzyme
activity? 23. In competitive inhibition:
I A. Substrate concentration A. Km increases
B. Enzyme concentration B. Km decreases
/Ii C. Time and pH C. Km remains the same
D. All of the above D. Km may increase or decrease
17. Enzyme increases the rate of reactions by: 24. Malonate is a competitive inhibitor of:
A. Decreasing the energy of activation A. Succinate dehydrogenase
B. Increasing the energy of activation B. Pyruvate dehydrogenase
C. Increasing the free energy change of the reaction C. Malate dehydrogenase
D. Decreasing the free energy change of the reaction D. Carbonic anhydrase
18. Km (Michaelis constant) is: 25. Which statements about allosteric enzymes is true?
A. The dissociation constant for the enzyme-substrate A. They are usually not controlled by feedback inhibition
complex B. The catalytic site is distinct from the allosteric site
B. The substrate concentration that gives one-half VmaX C. They change the nature of products formed
C. The molecular weight of an enzyme D · All of the above
D. None of the above
12 A 13 C 14 D 15 B 16 D 17 A 18 B 19
C 20 D 21 C 22 D 23 A 24 A 25 B
--
36 MCQs in Biochemistry for Paramedical Students Enzymes 37
26. Which enzyme is most sensitive to fluoride ion?
A. Enolase
--- Th diagnostic enzyme in muscular dystrophy is:
32· A ~reatine phosphokinase (CPK)/Creatine kinase (CK)
B. Aldolase
.. Lactate dehydrogenase (LDH)
C. Aconitase 8
D. Pyruvate kinase C. Alkaline phosphatase
o. Lipase
27. The active site of an enzyme:
. All the enzymes are increased in myocardial infarction
A. Binds covalently to substrate 33
B. Is composed of a linear amino acid array except:
C. Is small relative to the total bulk of the enzyme A.LDH
D. Is none of the above 8. SGOT
C.CPK
28. All the following mechanisms regulate enzyme activity o. Alkaline phosphatase
except:
34. Which of the following enzymes in serum is specifically
A. Changing the absolute quantity of enzyme
elevated in alcoholism?
B. Altering the pool size of reactants other than enzyme A. Acid phosphastase
C. Altering the catalytic efficiency of the enzyme 8. y-Glutamyl transpeptidase {y-GT)
D. Altering the temperature of reaction
C.SGOT
29. An example for functional plasma enzyme is: D.SGPT
A.LDH
35. Serum amylase is highly raised in:
B. Lipoprotein lipase
A. Diabetes mellitus
C. Amylase
B. Liver disorders
D. Acid phosphatase
C. Acute pancreatitis
30. Normal level of SGOT (AST) at 37°C is: D. Bone disorders
A. 0-40 IU/L 36. Isoenzymes (Isozymes):
B. 0-80 IU/L
A. Catalyse the same reaction
C. 60-180 IU/L B. Have different mobilities on electrophoresis
D. 100-250 IU/L C. Are physically distinct forms of the same enzyme
31. Acid phosphatase level in serum is elevated typically D. All of the above
in: 37. Number of isoenzymes of LDH in serum are:
A. Acute pancreatitis A. 2
B. Osteomalacia B. 3
C. Prostatic carcinoma c. 5
D. Obstructive jaundice D.6
26 A 27 C 28 D 29 8 30 A 31 C 3
2A 33A 348 35 C 360 37C
i I
... -·
---
C. <X-Tocopherol
D. 0-Tocopherol
6 B 7 D 8 A 9 B 10 C 11 A 12 C ~1~3:--::-D~-1-4-B~-1-5~8~~16~D~-1-7~A~-1-
8-C~~~
42 MCQs in Biochemistry for Paramedical Students Vitamins 43
2
:, 4 43 D 44 B 45 C 46 B 47 B 48 C
34 B 35 c 36 A 37 D 38 c 39 A 40 D D 50 A
41 B
PART Ill: METABOLISM
Biological Oxidation,
Introductory Metabolis Electron Transport Chain
1. All the following statements regarding metabolism an
and Bioenergetics
correct except:
A. It consists of anabolism (synthesis) and catabolisrr
(degradation) t. Oxidation is defined as:
B. It indicates the sequence of chemical reactions undergom A. Addition of oxygen
by the food from ingestion to excretion of metabolite B. Loss of hydrogen
.JI C. During anabolism, energy is liberated.
D. It can be studied by in vitro and in vivo methods
C. Loss of electrons
D. All of the above
I 2. Oxidoreductases involved in biological oxidation are
""' 2. All the following in vivo methods are used to stud)
metabolism except:
all of the following except:
A. Dehydrogenases
A. Studies with purified enzymes B. Hydroperoxidases
B. Use of radio active isotopes C. Transaminases
C. Respiratory exchange experiment D. Oxygeneses
D. Organ perfusion technique
3. Which one protects the body against damage by free
3. Major method to separate and purify biomolecules is: radicals?
A. Salt fractionation B. Ultra centrifugation A. Folic acid
C. Gel filtration D. All of the above B. Hydroperoxidases
4. Which radio active isotope is used to investigate C. Phosphatases
D · Oxygenases
carbohydrate metabolism?
4
A. 1311 B. 14C · ~Ydroxylation of drugs and steroids is effected by:
C. 59fe D. 24C · Oxygenases
8 · Superoxide dismutase
5. Radioactive isotope used to study thyroid function js: C. Catalase
A. 14C B. 32p D.FAD+
c. 1311 D. 45Ca ______.-/
1 D
1 c 2 A 3 D 4 B 5 c 2 c 3 B 4 A
48 MCQs in Biochemistry for Paramedical Students
D.6
B. Cori cycle c. 5
C. Respiratory chain The number of sites where ATP is produced in the
D. y-glutamyl cycle 12. h.
respiratory c am are:
6. Electron transport chain (respiratory chain) is locatec A.1 B. 3
in: c. 5 D. More than 5
A. Nucleus
B. Mitochondria 13. Cytochromes are enzymes which function as electron
transfer agent in:
C. Ribosomes A. Hydrolysis
D. Lysosomes B. Conjugation reactions
7. All of the following electron carriers are components o C. Transamination
the electron transport chain except: D. Oxidation and reduction
A. NADP+ 14. Oxidative phosphorylation is a process for:
B. NAD+ A. Phosphorylation of glucose
C. FAD B. Generating creatine phosphate
D. Coenzyme Q C. Generating ATP
8. Major components of electron transport chain all D. Utilizing ATP
arranged in order of redox potential which is: 15. All of the following are concerned with mechanism of
A. Decreasing oxidative phosphorylation except:
B. Increasing A. Conformational coupling hypothesis
C. Variable B. Chemiosmotic theory
D. Alternatively increasing and decreasing C. Chemical coupling hypothesis
9. Electron transport chain consists of how many roajo D. Beer-Lambert's law
complexes? 16
· Substances that can uncouple oxidation from
A. 7 B. 5 phosphorylation in electron transport chain include all
c. 3 D.4 the following except:
10. When the substrate enters the respiratory chain throu~
A. Barbiturates
B. 2, 4-Dinitrophenol
NAD linked dehydrogenase, the ATP yield is:
C. Pentachlorophenol
A.4 B. 3 D. Dinitrocresol
C. 2 D. 1
-------------------------~-:-:--:--~~~
11
A 12 B 13 D 14 C 15 D 16 A
5 c 6 B 7 A 8 B 9 D 10 B
-----
Biological Oxidation 51
50 MCQs in Biochemistry for Paramedical Students
17. Which one is not an inhibitor of oxidat· 23· Which one is a low energy compound?
.
phosphorylation?
~
A. Carbamoyl phosphate
A. Piercidin A B. Creatine phosphate
B. Cyanide c. AMP
C. 2,4-Dinitrophenol o. t,3-Bisphosphoglycerate
D. Rotenene
24. Phosphagen is:
18. Endergonic reactions are those which take place Wit} A. Creatine phosphate
A. Decrease of free energy B. Creatinine phosphate
B. Increase of free energy C. Glucose 3-phosphate
C. Decrease of activation energy D. Fructose 6-phosphate
D. Increase of activation energy 25. Energy currency of the cell is:
19. The energy content of ATP molecule is approximate!) A.GTP
A. 1000 calories B. ATP
B. 5000 calories C.ADP
C. 8000 calories D. Glucose
D. 12000 calories
20. The energy rich properties of ATP are related to:
A. Sugar groups
B. N-glycoside linkage
C. Heterocyclic ring
D. Phosphate anhydride linkages
21. The chemical energy required for the synthetic procesSt
is provided by:
A. Phosphorylation of ADP
B. Dephosphorylation of ADP
C. Phosphorylation of ATP
D. Dephosphorylation of ATP
22. All the following are high energy compounds except:
A. Phosphoenol pyruvate
B. Glucose-6-phosphate
C. ATP
D. Creatine phosphate
24 A 25 B
17 B 18 A 19 C 20 D 21 D 22 B
Di~tion , Absorption and Metabolism of COH 53
B. Stomach A. Phosphofructokinase
8 · Phosphohexose isomerase
C. Duodenum
D.Ileum C. Glucokinase
D. Pyruvate kinase
4. Which sugar is absorbed at the fastest rate from the sJl\~ 11
intestine? · Fluorid · h ' ·
A e in 1b1ts which enzyme in glycolysis?
A. Fructose · Aldolase
B. Glucose B. Hexokinase
C. Galactose ~· Elactate dehydrogenase
· nolase
D. Ribose
sa=------~:--~~~~~~~~~~~-=-~-:-:--:::-~~-
1 B 2 D 3 A 4 C s D 7 C 8 B g A 10 B 11 D
54 MCQs in Biochemistry for Paramedical Students Di~stion, Absorption and Metabolism of COH 55
12. What is the net ATP yield in glycolysis under aerob· - -CttnC -. -. acid cycle ta k
es place
"m:
•, ? lr 19· . .
con d 1hons. Lysosome B. Endoplasrmc reticulum
A.~ B.M ~: Mitochondria D. Cytosol
C. 8 D.2 TCA cycle is controlled by:
20
13. Net ATP yield in glycolysis under anaerobic conditioll: · A. ex Ketglutarate dehydrogenase
is: B. Isocitrate dehydrogenase
A. 1 B. 2 c. Citrate synthase
c. 10 D.12 o. All of the above
14. Rapaport-Leubering cycle involving glycolysis occun 21 . Substrate level phosphorylation in citric acid cycle is:
in: A. Succinyl CoA -7 Succinate
A. Erythrocytes B. Platelets B. Malate -7 Oxaloacetate
C. Hepatocytes D. Nerve cells C. Acetyl CoA -7 Citrate
D. Succinate -7 Fumarate
15. Lactate dehydrogenase catalyses:
A. Lactic acid H Acetic acid 22. All the following are inhibitors of citric acid cycle except:
B. Lactic acid H Pyruvic acid A. Malonate B. Arsenite
C. Lactic acid H Propionic acid C. Fluoride D. Fluoroacetate
D. Lactic acid H Acetyl Co A 23. ATP yield in citric acid cycle per turn is:
16. Which multienzyme complex is involved in th1 A. 8 B. 12
conversion of pyruvate to acetyl CoA? C. 24 D. 38
A. Pyruvate dehydrogenase 24. How many molecules of ATP are produced when one
B. Acetyl CoA carboxylase molecule of glucose gets completely oxidized to C0 2
C. Pyruvate kinase and H20?
D. ex Ketoglutarate dehydrogenase A. 8 B. 24
c. 38 D. 129
17. Pyruvate is converted to acetyl CoA by:
A. Decarboxylation 25. Glycogenolysis occurs in:
B. Oxidative decarboxylation A. Liver B. Muscle
C. C02 fixation C. Kidney D. All of the above
D. Oxidation 26. The glycogen branching enzyme is:
0
18. TCA cycle (Tricarboxylic acid cycle or citric acid cycle. A. Phosphorylase
11
Krebs cycle) is the final metabolic pathway by wh ' B. Amylo 1,6 glucosidase
acetyl CoA is completely oxidised to: C. Amylo O-t4)-t(6-71) transglucosidase
A. Carbon dioxide B. Water D. Amylo O-t6) glucosidase
C. Cabondioxide and waterD. Ammonia
21 A 22 C 23 B 24 C 25 D
12 C 13 B 14 A 15 B 16 A 17 B 18 C
56 MCQs in Biochemistry for Paramedical Students ·ion Absorption and Metabolism of COH 57
01gest '
27. Muscle glycogen cannot give rise to blood glucose d - - - - tion of glucose from noncarbohydrate precursors
34 fol'llla
to deficiency of: II • • called as:
l5 .
A. Branching enzyme A. Glycolys1s .
B. Debranching enzyme B. Glycogenes1s .
C. Glucokinase C Gluconeogenes1s
D. Glucose-6-phosphatase 0 '. Glycogenolysis
28. The enzyme deficient in von Gierke's disease is: Gluconeogenesis is reciprocally related to:
35. .
A. Glycogen synthase A. Glycolys1s
B. Glucose-6-phosphatase B. HMPShunt
C. Glucokinase c. Glycogenesis
D. Phosphorylase o. Glycogenelysis
29. Which of the following hormones promotes rapi. 36. Non carbohydrate precursors for gluconeogenesis are all
glycogenolysis in both liver and muscle? of the following except:
A. Epinephrine B. Glucagon A. Glycerol
C. Insulin D. ACTH B. Lactate
C. Alanine
30. Storage capacity of glycogen in a normal adult is:
D. Fatty acids
A. 72 g B. 295 g
C. 327 g D. 1800 g 37. All the following are key gluconeogenic enzymes except:
A. Pyruvate kinase
31. The tissue with the higher total glycogen content is:
8. Pyruvate carboxylase
A. Liver C. Glucose-6-phosphatase
B. Muscle D. Phosphoenol pyruvate carboxykinase
C. Brain
D. Kidneys 38. HMP shunt (pentose phosphate pathway) is located in:
A. Endoplasmic reticulum
32. Most of the energy for muscular contraction is stored I B. Cytosol
muscle as: C. Mitochondria
A. Creatine phosphate D. Peroxisomes
B. ATP 39
C. GTP
• HMP shunt is:
D. Phosphoenol pyruvate A. Breakdown of glycogen
8 · Synthesis of glycogen
33. Cori cycle (lactic acid cycle) involves: ~· Alternate pathway for oxidation of glucose
A. Blood B. Muscle · Synthesis of glucose from non carbohydrate sources
C. Liver D. All of the above
34
29 A 30 C 31 B 32 A 33 D C 35 A 36 D 37 A 38 B 39 C
27 D 28 B
58 MCQs in Biochemistry for Paramedical Students
01~stion, Absorption and Metabolism of COH 59
40. HMP shunt is significant in cellular metabolism beca ~poglycemic hormone produced by the pancreas
it produces: 14
is:
A. ATP B. Acetyl CoA A. Glucagon
C.NADH D.NADPH B. Insulin
41. Which of the metabolites is excreted in abnorlll1 C. Epinephrine
quantities in urine in essential pentosuria? o. Thyroxine
A. L-Xylulose • Normal level of true glucose in serum/plasma in fasting
47
B. L-Ribulose state is:
C. D-Ribose A. 15-45 mg/100 ml
D. L-Ribose B. 70-110 mg/100 ml
C. 80-120 mg/100 ml
42. Which of the following statement is not correct?
A. Uronic acid pathway is an alternative oxidati\ o. 150-250 mg/100 ml
pathway for glucose 48. Glucose tolerance test (GIT) is performed to assess:
B. Hereditary fructose intolerance is caused by deficienr A. Acinar functions of the pancreas
of aldolase B B. Acute pancreatitis
C. Essential fructosuria is due to deficiency of fructokinas C. Endocrine function of the pancreas
D. Vitamin C can be synthesised in humans in uronicaci D. Carcinoma of pancreas
pathway. 49. Glucose tolerance is decreased in:
43. UDP Galactose condenses with glucose to form: A. Hyperactivity of adrenal cortex
A. Glycogen B. Sucrose B. Hyperpituitarism
C. Maltose D. Lactose C. Diabetes mellitus
D. All of the above
44. Classic galactosemia occurs due to deficiency of th
enzyme: 50. Increased glucose tolerance is observed in:
A. UDPGal 4-epimerase A. Administration of insulin
B. Galactose l-P04 uridyl transferase B. Diabetes mellitus
C. Galactokinase C. Hyperthyroidism
D. Lactose synthase D. Hyperpituitarisrn
45. All the following operate to maintain blood gluco~
level except:
A. Liver
B. Muscles and kidney
C. Redblood cells
D. Hormones
40 D 41 A 42 D 43 D 44 B 45 c 46 B 47 B 48 C 49 D 50 A
·on Absorption and Metabolism of Lipids 61
t
p1~s~1~,..:..::.:..=-~~~~~~~~~~~~~-
12. The principal building block of fatty acid is: ~CoA carboxylase is the most important enzyme
A. Succinyl CoA . the regu1ati" on of·.
18. j\cety ·
B. Acetyl CoA J\. fatty acid ox1'da f10dn .
ill
C. Propionyl CoA holesterol degra ahon
B. C h .
D. Acetoacetyl CoA C. Cholesterol synt ~sis
13. The main source of NADPH for lipogenesis is: o. Fatty acid synthesis
A. Pentose phosphate pathway Elongation of long chain fatty acids occurs in:
19. . . ul
B. Glycolysis J\. Endoplasrruc retie um
C. Uronic acid pathway B. Lysosomes
D. Citric acid cycle c. Peroxisomes
14. Biosynthesis of fatty acid requires which vitamin?
o. Mitochondria
A. Riboflavin 20. Acylcarrier protein (ACP) is involved in the synthesis
B. Pyridoxine of:
C. Thiamin A. Phospholipids
D. Pantothenic acid B. Fatty acids
C. Glycogen
15. Which is the rate limiting step in fatty acid synthesisl
D. Triglycerides
A. Pyruvate ~ Acetyl CoA
B. Acetyl CoA ~ Malonyl CoA 21. The main factor controlling the rate of lipogenesis is:
il.! C. Malonyl CoA ~ Acetoacetyl CoA A. Hormones
D. Acetoacetyl CoA ~ p hydroxy acetyl CoA B. Nutrition
C. Cyclic AM...0
16. All the following enzymes are multifunctional enzyme D. None of the above
complexes except:
A. Acetyl CoA carboxylase 22. Ketone bodies are formed during high rate of oxidation
/111 B. Fatty acid synthase of:
C. a-keto glutarate dehydrogenase A. Cholesterol
D. Pyruvate dehydrogenase B. Triglycerides
C. Fatty acids
0
17. The fattyacid synthase complex comprises tW D. Phospholipids
monomers, each containing: 23 · All the f 11 ·
A. 2 enzymes · except:
o owmg are ketone bodies
B. 5 enzymes A. Acetic acid
B. Acetone
C. 7 enzymes
D. 10 enzymes C. Aceto acetic acid
D. ~ hydroxy butyric acid (3-Hydroxybutyrate)
12 B 13 A 14 D 15 B 16 A 17 C 19 A 20 B 21 B 22 C 23 A
I II
. I
64 MCQs in Biochemistry for Paramedical Students ·on Absorption and Metabolism of Lipids 65
Dl~5t ·~,~=-=-:__.:....-----------'----
24. Ketone bodies are formed in mitochondria of: ~of the following is essential for absorption of
A. Kidney B. Spleen 31· WbtC ?
cholesterol.
C. Brain D. Liver A· Vitamin D
25. Ketone bodies cannot be utilized by: B. Extrinsic factor
A. Liver B. Kidney c. Bile
C. Muscle D. Brain o. None of the above
The organ which contains maximum quantity of
26. Ketosis occurs in: 32. .
A. Prolonged starvation cholesterol is:
B. High fat feeding A. Brain
C. Severe diabetes mellitus B. Liver
C. Kidney
D. All of the above
D. Skin
27. The test to detect ketone bodies in the urine is: 33. The greater part of cholesterol in plasma is present as:
A. Benedict's test
A. LDL
B. Xanthoproteic test
C. Rothera's test
B. VLDL
C. Free cholesterol
D. Jaffe' s reaction
D. Cholesterol esters
28. Prostaglandins are synthesised in all the fo llowin! 34. Cholesterol biosynthesis takes place in:
organs except: A. Golgi apparatus
A. Seminal vesicles B. Microsomal fraction and cytosol
B. Thymus C. Ribosomes
C. Lung D. Mitochondria and cytosol
D. Spleen
35. Starting compound for cholesterol biosynthesis is:
29. Prostaglandins are formed via: A. Acetyl CoA
A. Kynurenate pathway B. Acetoacetyl CoA
B. Cyclooxygenase pathway C. Propionyl CoA
C. Uronic acid pathway D. Succinyl CoA
D. Lipoxygenase pathway 36
· The regulatory enzyme in cholesterol biosynthesis is:
30. Synthesis of prostaglandins is inhibited by: A. HMG CoA synthase
A. Aspirin B. HMG CoA oxidase
B. Arsenate ~· HMG CoA reductase
C. Arsenite · 7a.-Hydroxylase
D. All of the above
31 c 32 A
24 D 25 A 26 D 27 C 28 D 29 B 30 A 33 D 34 B 35 A 36 c
66 MCQs in Biochemistry for Paramedical Students ion Absorption and Metabolism of Lipids 67
Di~ 5t ~'~~~--------.:....__ _
37. All the compounds are formed from cholesterol except. ~(Bad cholesterol) is formed from:
A. Bile acids · 44· ~.VLDL
B. Bile pigments
C. Vitamin D
D. Steroid hormones
c:B chlomicrons
Both of the above
p.HDL
38. The main catabolic end product of cholesterol is: VLDL contains the principal apoprotein:
A. Acetyl CoA B. Propionyl CoA 45. A. Btoo B. C.I
C. Coprosterol D. Bile acids C. B4s D. C-11
39. Normal level of cholesterol in serum is:
A. 70-110 mg/dl 46. Fatty liver occurs in:
A. Malnutrition
B. 15-45 mg/dl B. Choline deficiency
C. 150-250 mg/ dl c. Alcoholism
D. 60-160 mg/dl D. All of the above
40. Serum cholesterol level is elevated in: 47. All the following are lipotropic factors except:
A. Hypothyroidism A. Methionine
B. Nephrotic syndrome B. Betaine
C. Atherosclerosis C. Vitamin B12
D. All of the above D. Choline
41. An example for hypocholesterolemic drug is: 48. Which one of the following is an inborn error of fat
A. Mevastatin metabolism?
B. Erythromycin A. Pompe's disease
C. Digitonin B. Newmann-Pick disease
D. Streptomycin C. Lesch Nyhan syndrome
D. Albinism
42. Which lipoprotein has a beneficial effect against
atheroscreosis? 4
9. Which of the following accumulates in Gaucher's
A. IDL B. LDL disease?
C. VLDL D.HDL A. Spingomyelins B. Cerebrosides
43. Good Cholesterol is: C. Glycogen D. Copper
A. VLDL Cholesterol SO. Which one is defective in familial hypercholesterolemia?
B. HDL Cholesterol A. LDL receptor
C. LDL Cholesterol B. liDL receptor
D. Chylomicrons C. VLDL receptor
D. liMG-CoA reductase
37 B 38 D 39C 40 D 41 A 42 D 43 B 44 A 45 A 46 D 47 C 48 B 49 B 50 A
p~tion, Absorption and Metabolism of Proteins 69
1 D 2 c 3 D 4 B SA 6 A 7 B 8 A 9 C 10 D
70 MCQs in Biochemistry for Paramedical Students . Absorption and Metabolism of Proteins 71
pl~1on.
~any
11. Urea synthesis takes place mainly in:
A. Muscle
B. Liver
17· ao;
syn e
molecules of ATP are required for the
sis of 1 molecule of urea in urea cycle?
B. 3
C. Kidneys A.1 D.8
D. All of the above
c. 5
• Th link between TCA cycle and urea cycle is through:
12. The reactions of the Krebs-Hensleit's urea cycle occll! 1s. e
A. Citrate
in the: B. Pyruvate
A. Mitochondrial matrix c. fumarate
B. Cytosol o. Malate
C. Mitochondrial matrix and cytosol
D. Ribosomes 19. All the following are inborn errors of metabolism
associated with urea cycle except:
13. Biosynthesis of urea begins with the formation of A. Hyperargininemia
carbamoyl phosphate from: B. Type II Orotic aciduria
A. Carbon dioxide and ammonia C. Hyperammonemia Type 2
B. Carbon dioxide, ammonia and GTP D. Argininosuccinicacid uria
C. Carbon dioxide, ammonia and ATP
20. Citrullinemia occurs due to absence of the enzyme:
D. Succinyl Co A and glycine
A. Arginosuccinase
14. Which amino acid is not involved in urea cycle? B. Arginosuccinate synthase
A. Arginine C. Glutamine synthetase
B. Aspartic acid D. Ornithine transcarbamoylase
C. Glycine
21. The major mechanism for the removal of ammonia in
D . Ornithine
the liver is through formation of:
15. The regulatory enzyme in urea cycle is: A. Uric acid
A. Carbamoyl phosphate synthase II B. Urea
B. Carbamoyl phosphate synthase I C. Creatinine
C. Arginase D. Glutamine
· ~ormation of glutamine is catalysed by which enzyme
D. Urease 22
16. Citrulline is formed by the combination of carbam0Y1 11\ kidney?
phosphate with: A. Glutaminase
8 · Glutamate dehydrogenase
A. Ornithine
B. Arginine C. Glutamine synthetase
C. Choline D. Glutamine oxidase
D. Cysteine
17 B c
11 B 12 c 13 C 14 D 15 B 16 A 18 19 B 20 B 21 B 22 c
72 MCQs in Biochemistry for Paramedical Students Absorption and Metabolism of Proteins 73
[)19!!!!on,
23. In brain, ammonia is detoxified mainly by fonnaij ~ne
'('hre0n1
can be catabolised to:
~ % 30· Propionyl CoA
A. Glutamine B. Asparagine :: Acetyl CoA
C. Urea D . Creatinine C Succinyl CoA
24. The symptoms of ammonia intoxication include: 1).• None of the above
A. Blurring of vision smethylation of guanidoacetic acid gives:
1
B. Tremor 3t. ~-methyl nicotinamide
C. Slurred speech B. Creatine phosphate
D. All of the above c. Crea tinine
25. A glycogenic amino acid is: o. Betaine
A. Leucine
32• The active form of methionine is:
B. Isoleucine A. 5-acetyl methionine
C. Methionine B. N-acetyl methionine
D. Phenylalanine C. 5-carboxyl methionine
D. 5-adenosyl methionine
26. Which among the following is a ketogenic amino acid1
A. Tyrosine 33. Polyamines are formed from:
B. Arginine A. Arginine B. Histidine
C. Isoleucine C. Alanine D. Phenylalanine
D. Leucine
34. All the following are formed from glycine except:
27. Tryptophan is a: A. Purines B. Heme
A. Glycogenic amino acid C. Spermine D. Creatinine
B. Nonessential amino acid
35. Histidine can be converted to histamine (vasodilator)
C. Glycogenic and ketogenic amino acid by:
D. Ketogenic amino acid A. Carboxylation
28. An immediate precursor of glycine is: B. Decarboxylation
A. Choline B. Serine C. Transamination
C. Lysine D. Valine D. Oxidative deamination
29. Serine can be synthesised from:
36
· ~ABA. (y-Aminobutyrate), an inhibitory neurotrans-
A. 3-phospho glycerate tter is formed by decarboxylation of:
B. Glycine A. Pyruvic acid
C. Both of the above B. Glutamic acid
D. Tyrosine C. Glutamine
D· Aspartic acid
23 A 24 D 25 C 26 D 27 C 28 B 29 C 31 B 32 D 33 A 34 C 35 B 36 B
74 MCQs in Biochemistry for Paramedical Student Absorption and Metabolism of Proteins 75
~on,
~
37. Forminoglutamic acid (FIGLU) is formed from: ~·ne
'fhytOXl
is formed from which amino acid?
A. Methionine B. Tyrosine
44. A· Glycine
C. Threonine D. Histidine
8. Tryptophan
38. Maple syrup urine disease is an inborn error c. Tyrosine
metabolism of: 01
o. Threonine
A. Aromatic amino acids .Albinism occurs due to deficiency of the enzyme:
B. Branched chain amino acids 45, .
A. Tyrosmase .
C. Sulphur containing amino acids . Homogentisate ox1dase
D. Urea cycle 8
c. Phenylalanine hydroxylase
39. The test to detect phenylketonuria is: o. Aldolase B
A. Ferric chloride test 46. Catabolism of tryptophan produces:
B. Benzidine test A. Melanin B. Serotonin
C. Fouchet's test c. Urocanate D. Nicotinate
D. Rothera's test
47. Deficiency of the enzyme phenylalanine hydroxylase
40. All the following compounds are formed from tyrosine results in:
except: A. More excretion of alanine in urine
A. Norepinephrine B. Alkaptonuria
B. Melanin C. Phenylketonuria
C. Melatonin D. Hartnup disease
D. Epinephrine
48. Phenylketonuria (PKU) is characterized by:
41. Carcinoid syndrome occurs due to abnormal metabolism A. Urine becoming dark on standing
of: B. Urine with a mousy odour
A. Tyrosine B. Tryptophan C. Urine with smell of burnt sugar
C. Methionine D. Proline D. Increased tyrosine level in urine
49
42. Epinephrine is formed from norepinephrine by: • Alkaptonuria is due to deficiency of which enzyme?
A. Decarboxylation A. Dopa decarboxylase
B. Hydroxylation B. Phenylalanine hydroxylase
C. Transamination ~· Homogentisate oxidase
D. N-methylation · Tyrosinase
43. 5-Hydroxy indole acetic acid is the metabolic end produd SO. All th f
e ollowing compounds are formed due to
of: :"~fa~tion of aminoacids in the large intestine except:
A. Serotonin
C. Histidine
B. Adenylic acid
D. Cholesterol
c:Hl~c oxide
2
B. Indole
D . Tyramine
37 D 38 B 39 A 40 C 41 B 42 D 43 A 45 B 46 B 47 C 48 B 49 c 50 A
Digestion, Absorption and Metabolism n
. ci·d synthesis mainly takes place in:
uric a .
s. Muscle B. Kidneys
~: Liver D. Bone marrow
1111 ~1 1 6
. Uric acid is fo~ed from xanthine by the action of:
A. Xanthine ox1dase
Digestion, Absorption B. Xanthine reductase
c. Urease
and Metabolism of o. Uricase
I' Nucleic Acids 1
Sodium urate crystals are deposited in soft tissues in
· gout and these urate deposits are referred to as:
A. Calculi B. Granules
C. Tumor D. Tophi
1. All the following enzymes are involved in digestion of s. A drug which prevents uric acid synthesis by inhibiting
dietary nucleic acids except: the enzyme xanthine oxidase is:
A. Restriction endonucleases A. Rifampicin B. Aspirin
B. Ribonucleases C. Allopurinol D. Digitonin
C. Nucleosidases 9. Which enzyme deficiency leads to hypouricemia?
D. Deoxyribonucleases A. Carbamoyl phosphate synthase II
B. Xanthine oxidase
2. Which one is a purine nucleotide?
C. Orotidylic acid decarboxylase
A. Uridylate
D. Glutamate dehydrogenase
B. Cytidylate
C. Adenylate 10. Hyperuricemia occurs in all the following disorders
D. Thymidylate except:
A. Von Gierke's disease
3. The chief end product of purine catabolism in humill1
B. Orotic aciduria
is: C. Gout
A. Xanthine B. Uric acid
D. Lesch-Nyhan syndrome
C. Allantoin D. Urea
ll. Normal level of serum uric acid in men is:
4. Which is the end product of purine catabolisnt in A. 3-7 mg/100 ml
mammals other than higher primates? B. lS-45 mg/100 ml
A. Urea C. 70-110 mg/100 ml
B. Uric acid D. 6-8 g/100 ml
C. Ammonia
D. Allantoin
6 A 7 D 8 C 9 B 10 B 11 A
1 A 2 c 3 B 4 D
78 MCQs in Biochemistry for Paramedical Stu den~ Digestion, Absorption and Metabolism 79
12. Uric acid pool in human body is about: Jllan the chief end products of pyrimidine catabolism
A. 7 mg B. 700 mg 19· In
are=
C. 1200 mg D. 2 g A· ~-amino!sobutyrate B. Carbon dioxide
C. Ammonia D. All of the above
13. During denovo purine biosynthesis the firs t pu .
nucleotide formed is: rin, Which one of the following is an inborn error of
A. Xanthylic acid B. Inosinic acid 20. pyriJ11idine meta b ol"ism.?
C. Guanylic acid D. Adenylic acid A. Lesch-Nyhan syndrome
14. What are the atoms of purine that are derived fro 8. Orotic aciduria
glycine? in c. Gout
A. C4, CS and N7
o. Methylmalonic aciduria
B. C4, C6 and N7
C. C4, CS and N9
D. C3 and C9
15. Purine biosynthesis is regulated by:
A. Phosphoribosyl pyrophosphate (PRPP)
B. Phosphoribosylamine
C. Ribose phosphate
D. Inosine monophosphate
16. Purine biosynthesis is inhibited by:
A. Methotrexate B. Azaserine
C. Aminopterin D. All of the above
17. In salvage pathway, purine nucleotides are formed front
A. Purines and ribose-1-phosphate
B. Purines and phosphoribosyl pyrophosphate
C. Purines and ribose S-phosphate
D. Purines and ribose
18. The first step in pyrimidine biosynthesis is catalyseo
by:
A. Carbamoyl phosphate synthase I
B. Carbamoyl phosphate synthase II
C. Orotidylic add decarboxylase
D. Adenosine deaminase
12 C 13 B 14 A 15 A 16 D 17 A 18 B 20 B
Metabolism of Hemoglobin 81
sa 6 c
1 c 2 B 3 A 4 C 7 C 8 A 9 B 10 A
82 MCQs in Biochemistry for Paramedical Students Metabolism of Hemoglobin 83
11. Acquired porphyria may occur due to: --:::oglobin biosynthesis the first to react are:
A. Toxic chemicals 11· Glycine and formate
B. Diabetes mellitus A· Glycine and Propionyl CoA
C. Cancer ~: Glycine and Suc~inyl CoA
D. Tuberculosis p. Lysine and Succmyl CoA
12. Test for porphobilinogen in porphyrinuria is: eme is also required for synthesis of:
A. Hay's test 18 H
· A. 1mmunog1obul"ms
B. Watson and Swartz test
8 . Cyanocobalamin
C. Fouchet's test c. Ceruloplasmin
D. Benzidine test o. Cytochromes
13. Faeces normally contain: l9. Degradation of hemoglobin takes place in:
'" l il A. Coproporphyrin Type I A. Gallblad der
B. Coproporphyrin Type III 8. Reticuloendothelial system cells
C. Uroporphyrin III C. Kidneys
D. Uroporphyrin I D. Liver
14. Synthesis of hemoglobin mainly occurs in: 20. In a normal adult, the quantity of hemoglobin
A. Mature erythrocytes catabolised everyday is:
B. Kidney A.4gm
C. Spleen 8. 6gm
D. Liver and immature erythrocytes C. 12 gm
D.18 gm
15. The cell organelle(s) involved in the synthesis of
hemoglobin is: 21. Catabolism of hemoglobin produces:
A. Cytosol A. Porphobilinogen
B. Mitochondria B. Bile acids
C. Mitochondria and cytosol C. Acetyl CoA
D. Ribosomes D. Bile pigments
16. Heme synthesis involves the incorporation of ferrous 22. ~e first b ile pigment formed from the degradation of
iron (Fe++) into protoporphyrin III in a reaction catalysed e111oglobin is:
by the enzyme: A. Biliverdin
A. Ferrochelatase B. Bilirubin
B. Ferroreductase C. Urobilinogen
C. Ferrooxidase D. Stercobilin
D. Hemeoxygenase
11 A 12 B 13 A 14 D 15 c 16 A
17
c~~,a=-0~~1-9-0~~20~-e~-2-1----=-o~-2~2=--A=--~~~~~
Metabolism of Hemoglobin 85
84 MCQs in Biochemistry for Paramedical Stude
~ 'J!J. sUitubin is increased in all the following disorders
23. Which of the following are bile pigments?
A. Cholic acid and deoxycholic acid IXcept:
B. Bilirubin and biliverdin A· pubin-Johnson syndrome
Gilbert's syndrome
C. Bilirubin and cytidine
D. Biliverdin and avidin c:
8
McArdle syndrome
o. Crigler-Najjar syndrome
24. Bilirubin is converted to biliverdin by:
A. Oxidation 3(). Which enzyme is deficient in Gilbert's syndrome?
A. UDP glucuronyl transferase
1 B. Conjugation
I 1
C. Reduction
8. UDP galactose pyrophosphorylase
I I
c. UDP glucose epimerase
~
D . Hydroxylation
o. Biliverdin reductase
II 11 25. Bilirubin formed in reticuloendothelial system ii
transported to liver in combination with:
A. Albumin
B. Globulin
C. Transferrin
D. Ceruloplasmin
26. Defective binding of bilirubin by albumin results in:
A. Porphyrias
B. Kernicterus
C. Both of the above
D. Anemia
27. The enzyme responsible for conjugation of bilirub~
is:
A. Hemoglobin reductase
B. Bilirubin hydroxylase
C. Bilirubin esterase
D. Glucuronyl transferase
28. Jaundice occurs due to increased concentration of:
1~:~1 A. Bile acids
B. Bile pigments
C. Ketone bodies
I D. Bile salts
30 A
23 B 24 C 25 A 26 B 27 D 28 B
Metabolism of Minerals 87
8 c 9 B 10 D 11 C 12 C 13 B
1 c 2 A 3 D 4 B 5 B 6 D
88 MCQs in Biochemistry for Paramedical Students Metabolism of Minerals 89
14. Calcium is necessary for the activation of the enzYnt Magnesium is an essential component in:
A. A TPase B. Fumarase e: 21· Vitamin B12 B. Hemoglobm
C. Glucokinase D. Xanthine oxidase ~: Ceruloplasmin D. Chlorophyll
15. Normal calcium level in serum is: Magnesium deficiency causes:
A. 0.6-1.2 mg% 22- A. Convu1s10ns
.
B. 3-5 mg%
B. Anemia
C. 6-20 mg% c. Sterility
D. 8.5-10.5 mg% o. Retarded growth
16. Which one of the hormones regulates blood calciurn
level? 23• The principal cation in the extracellular fluid is:
A. Potassium B. Sodium
A. Thyroid hormones c. Magnesium D. Chloride
B. Parathyroid hormones
C. Insulin 24. Total iron content of a normal adult is:
D. Glucagon A. 1-2 g B. 2-4 g
C. 5-7 g D. 8-10 g
17. Total phosphorus present in the body is:
A. 300 g B. 700 g 25. Milk is deficient in which of the following minerals?
C. 1.1 g D. 1.5 kg A. Calcium
8. Iron
18. The richest source of phosphorus in the diet is: C. Sodium
A. Eggs D. Phosphorus
B. Milk
C. Cheese 26. Iron is mainly absorbed from:
D. All of the above A. Ileum
B. Caecum
19. Sulphur is a component of: C. Stomach
A. Thiamine D. Duodenum and Jejunum
B. Vitamin B12 27
C. Vitamin E • Iron absorption can increase due to:
D. None of the above A. Glutathione B. Gastric HCl
C. Vitamin C D. All of the above
20. Sulphur is excreted in urine as: 28
• Bronze diabetes is associated with abnormal metabolism
A. Neutral sulfur of:
B. Inorganic sulphates A. Carbohydrates
C. Ethereal sulphates B. Iron
D. All of the above C. Chromium
D. Fats
21 D'~~~~~~~~~~~~~~~~~~~~
14 A 15 D 16 B 17 C 18 D 19 A 20 D 28 B 22 A 23 B 24 B 25 B 26 D 27 D
90 MCQs in Biochemistry for Paramedical Students Metabolism of Minerals 91
29. Percentage of total iron of human body p resent 1. --;:--is a constituent of the enzyme:
hemoglobin is: 1\ 3'· A· Transketolase
A. 10% B. 20% B. Carbonic anhydrase
C. 75% D. More than 75% C Lactate dehydrogenase
o'. Xanthme oxidase
30. Iron is present in all the following except:
A. Coenzyme A 7 Zinc deficiency
3.
causes:
B. Myoglobin A. Hypogona d ism
'
C. Peroxidases B. Hypothyroidism
D. Ca talases c. Anemia
o. Goitre
31. Transferrin, the transport form of Iron is a:
A. Mucopolysaccharide 38. Rich source for Iodine is:
B. Simple protein A. Milk
C. Compound lipid B. Sea water
D. Glycoprotein C. Meat
D. All of the above
32. Recommended daily allowance (RDA) of iron for
women during pregnancy and lactation is: 39. Iodine is stored in the form of:
A. 10 mg B. 18 mg A. Ferritin
C. 28 mg D. 38 mg B. Transferrin
C. Ceruloplasmin
33. All the following are copper containing proteins/ D. Thyroglobulin
enzymes except:
A. Superoxide dismutase 40. Deficiency of which mineral results in endemic goitre?
A. Fluorine
B. Erythocuprein
B. Selenium
C. Cytochrome oxidase
C. Iodine
D. Glutathione peroxidase
D. Manganese
34. Molybdenum is a component of: 4
A. Xanthine oxidase 1. All the following are goitrogenic substances except:
A. Sea foods
B. &-ALA dehydratase
B. Raddish
C. Cerebrocuprein
C. Cabbage
D. Alkaline phosphatase
D. Cauliflower
35. Wilson disease (hepatolenticular degeneration) results
due to defective metabolism of: 42. Main source of fluoride for human beings is:
A. Vegetables B. Eggs
A. Potassium B. Iron
C. Water D. Milk
C.CoppB D . fudme
~~~~~~~~~~~~~~~~~~ 38 B 37 A
29 C 30 A 31 D 32 B 33 D 34 A 35 C 38 B 39 D 40 c 41 A 42 c
92 MCQs in Biochemistry for Paramedical Students
----·
98 MCQs in Biochemistry for Paramedical Students
5. The caloric value of carbohydrate per gram is: • sMR is b elow normal in:
A. 4.1 C B. 5.4 C 13 A· Addison's disease B. Starvation
C. 7.1 C D . 9.3 C c. Both of the above D. Hyperthyroidism
6. Respiratory quotient (R.Q) is highest for: In which d isorder, BMR is above normal?
14
A. Proteins B. Carbohydrates · A. Polycythemia B. Leukemia
C. Fats D. Mixed diet c. Hyper thyroidism D. All of the above
7. R.Q of protein is: 15. The energy expended by an individual depends on:
A. 0.65 B. 0.85 A.BMR
c. 0.80 D. 1.00 S. SDA
c. Physical activities
8. Which food stuff has highest specific dynamic action?
D. All of the above
A. Vitamins B. Carbohydrates
C. Fats D. Proteins 16. Daily calorie requirement of an adult male is:
A. 1000 C B. 2500 C
9. Normal BMR of an adult male is:
C. 3000 C D. 4000 C
A. 10 C/sqm/hr
B. 20 C/ sqm/hr 17. Daily caloric requirement for a man doing moderate
C. 40 C/ sqm/hr work is:
D. 60 C/sqm/hr A. 2000-2400 C B. 2700-3200 C
10. BMR can be measured by:
c. 3200-4000 c D. Above 4000 C
A. Spectrophotometer 18. Energy expenditure per hour for doing light exercise is:
B. Colorimeter A. 170 C B. 250 C
C. Calorimeter C. 400 C D. 600 C
D. Benedict Roth apparatus 19. Recommended daily caloric requirement of lactating
11. BMR is influenced by the factors like: women is:
A. Climate A. 1500 C B. 2000 C
B. Age C. 3000 C D. 4500 C
C. Sex 20. Which activity requires most energy?
D. All of the above A. Swimming
12. Determination of BMR helps in the diagnosis of: B. Walking upstairs
A. Thyroid diseases C.Running
B. Diabetes Insipidus D. Walking very fast
C. Diabetes Mellitus
D. None of the above
5 A 6 B 7 C 8 D 9 C 10 D 11 D 14 D 15 D 16 B 17 C 18 A 19 C
12 A
Food and Nutrition 101
13. Which is the limiting aminoacid in pulses? Milk is rich in all the following except:
20. 1.
A. Methionine B. Lysine A· Ca c1um
C. Glycine D. Tyrosine 8. Phosphate
14. High quality proteins/first class proteins/comp} t
c. Iron
.
proteins are:
ee o. Sodium
A. Dairy products . Green leaf vegetables are good sources of:
21
B. Eggs A. Minerals
C. Meat 8. Vitamins
D. All of the above C. Carbohydrates
13 A 14 D 15 A 16 A 17 c 18 B 19 D 20 c 21 B 22 D 23 D 24 B 25 c
----
Fluid and Electrolyte Balance 105
c. Phosphate mechanism
Acid Base Balance o. All of the above
s. All of the following conditions may give rise to acidosis
except:
1. Normal pH of arterial blood is: A. Pyloric stenosis
A. 6.35-6.45 B. 7.35-7.45 B. Diarrhoea
C. 4.35-4.45 D. 6.95-7.25 c. Diabetic ketoacidosis
D. Fanconi's syndrome
2. If the pH of blood is 7.4 the ratio of NaHC0 3/ll 2CQ3
will be: 9, Alkalosis occurs due to:
A. 5:1 B. 10:1 A. Cushing' s syndrome
c. 20:1 D. 25:1 B. Pyloric stenosis
C. Hyperventilation
3. Mechanisms for regulation of acid base balance include: D. All of the above
A. Renal mechanism
B. Respiratory mechanism 10. Anion gap refers to the following in serum:
C. Buffer systems in the blood A. Unmeasured anions
D. All of the above B. Unmeasured cations
C. Difference between anions and cations
4. Factors which affect acid base balance include: D. Unmeasured anions and cations
A. Anaerobic energy production
B. Violent muscular exercise
C. A high protein diet
D. All of the above
5. The chief physiological buffer in the blood is:
A. Bicarbonate buffer B. Hemoglobin buffer
C. Proteinate buffer D. Phosphate buffer
6. The major acid produced in the body during oxidatioll
in the cells is:
A. Phosphoric acid B. Acetic acid
C. Carbonic acid D. Hydrochloric acid
1 B 2 c 3 D 4 D 5 A 6 c 7 D 8 A 9 D 10 C
----
Organ Function Tests 109
1 A 2 c 3 B 4 B 5 D ------------~~~~~~~~~~-
&D 7 c 8 A 9 D 10 D 11 B 12 D
11 O MCQs in Biochemistry for Paramedical Students
13 A 14 C 15 C 16 B 17 D 18 C 19 A 1 B 2 c 3 D 4 B 5 D
20 c
----
112 MCQs in Biochemistry for Paramedical Students Hormones 113
6. Which one is not a hypothalamic releasing factor? Which of the following hormones is involved in
13
A. LH-RF • increased reabsorption of water from renal tubular
B. Insulin-RF epithelial .cells?
C. Prolactin-RF A. Oxytocm
D.FSH-RF B. Vasopressin (ADH)
c. Cortisol
7. All the following hormones are produced by the anterio o. Glucagon
lobe of pituitary gland except: r
A.MSH 14. Diabetes insipidus occurs due to abnormal secretion or
B. TSH action of:
C. Prolactin A. Aldosterone B. Insulin
D. Growth hormone C. ADH D. Oxytocin
8. Growth hormone stimulates the growth of bones and 15. The hormone produced by middle lobe of pituitary is:
cartilages through: A. TSH B. MSH
A. Calcitonin C. LH D.FSH
B. Calmodulin 16. Hormone derived from tyrosine includes which of the
C. Somatomedin C following ?
D. None of the above A. Glucagon B. Thyroxine
9. All the following are pituitary tropic hormones except: C. Gastrin D. Oxytocin
A.ACTH B. ADH 17. All are antithyroid drugs except:
C. TSH D. Prolactin A. Thiouracil B. Thiourea
10. Lactogenic hormone is: C. PABA D. Alloxan
A. Oxytocin B. FSH 18. A disease which involves abnormalities in hormone
C. LH D. Prolactin receptor function is:
11. Oxytocin: A. Graves' disease
A. Is secreted by posterior lobe of pituitary B. Addison's disease
B. Causes uterine contraction C. Hartnup disease
C. Causes milk secretion D. Wilson's disease
D. All of the above 19
· Hyperthyroidism results in:
12. Which hormone is produced by posterior pituitary gland? A. Cushing' s syndrome
A. Oxytocin B. Grave's disease
B. Vasopressin C. Addison's disease
C. Both of the above D. Pheochromocytoma
D. Prolactin
6 B 7 A 8 C 9 B 10 D 11 D 12 C 14 C 15 B 16 B 17 D 18 A 19 B
114 MCQs in Biochemistry for Paramedical Students
----
Hormones 117
~
33. Which of the following stimulates the rel eas Which is the immediate precursor of testosterone?
e Of 39. .
aldosterone? Ji. Tyrosine
A. Angiotensin II B. Cholesterol
B. ACTH c. Progesterone
C. Both of the above o. Androstenedione
D.Calcium
40. Estradiol is a:
34. Which hormone stimulates both anabolic and cataboli }i. Steroid hormone
effects on protein metabolism? c B. Protein hormone
A. Glucocorticoids c. Peptide hormone
B. Insulin o. Derivative of aminoacid
C. Testosterone
D. Thyroid hormones 41. Pregnadiol is:
A. A secretion from the ovary
35. Epinephrine is synthesised from: B. An excretory product of progesterone
A. Tryptophan C. A component of a female contraceptive
B. Tyrosine D. Formed from prostaglandin
C. Glycine
D. Arginine 42. hCG (Human chorionic gonadotropin) is a tumor marker
of:
36. Cateholamines are all the following except: A. Thyroid
A. Adrenaline (epinephrine) B. Colon
B. Noradrenaline (norepinephrine) C. Lungs ,
C. Dopamine D. Trophoblast and germ cells
D. Serotonin
43. All steroid hormones are formed from:
37. Which one is an indolamine? A. Arachidonic acid
A. Adrenaline B. Acetyl CoA
B. Noradrenaline C. Glycine
C. Serotonin D. Cholesterol
D. Glutamine
44. Erythropoietin is a hormone secreted by:
38. Which of the following substances is present in hi·g~ A. Erythrocytes B. Corpus luteum
concentration in the urine of patients wit C. Kidney D. Pineal gland
pheochromocytomas (tumors of adrenal medulla)? 45
A. 3-Methoxy 4-0H mandelic acid · All of the following are gut hormones except:
A. Secretin B. Calcitonin
B. 5-Hydroxy indole acetic acid
C. Epinephrine
C. Motilin D. Cholecystokinin
D. Norepinephrine -------
33 A 34 A 35 B 36 D 37 c 38 A 39 D 40 A 41 B 42 D 43 D 44 c 45 B
----
Molecular Biology 119
7
1 c 2 A 3 D 4 B 5 c 6 B C 8 A 9 D 10 A 11 D 12 C
120 MCQs in Biochemistry for Paramedical Students
B. Heme
121
A. Fanconi's anemia
B. Pernicious anemia c. Protein D. Prostaglandins
C. Albinism
21. Genetic code was deciphered by:
D. Gaucher's disease A. Watson and Crick
14. Transcription is the formation of: B. Stahl and Meselson
A. RNA from DNA C. Nirenberg and Mathei
B. DNA from DNA D. Khorana
C. Protein from RNA 22. Codons are present in:
D. DNA from RNA A. rRNA B. mRNA
15. Transcription occurs in: C. tRNA D.DNA
A. Ribosomes B. Nucleus 23. Total number of codons in the genetic code is:
C. Golgi apparatus D. Mitochondria A. 1 B. 3
16. Which is not required for transcription? C. 61 D.64
A. RNA dependent DNA polymerase 24. Degeneracy of the genetic code denotes the existence
B. A DNA template of:
C. DNA dependent RNA polymerase A. Multiple codons for a simple amino acid
D. Sigma factors B. Base triplets that do not code for any amino acid
17. Primary transcript consists of: C. Codons consisting of only two bases
A. Exons D. Codons which contain one or more unusual bases
B. Introns 25. The initiating codon for protein synthesis is:
C. Both of the above A.UAG B. UUU
D. Okazaki fragments C.AAA D.AUG
18. RNA processing includes: 26. The chain initiating amino acid in protein synthesis in
A. 5' capping and poly A tailing E-coli is:
B. Methylation A. Glycine B. Methionine
C. Splicing of intrans C. Cysteine D. Arginine
D. All of the above
27. Non-sense codons bring about:
19. Amanitin (mushroom poison) inhibits: A. Initiation of protein synthesis
A. Replication B. mRNA synthesis B. Elongation of polypeptide chain
C. Protein synthesis D. ATP synthesis C. Termination of protein synthesis
~ostranslational modification of proteins
20 C 21 C 22 B 23 D 24 A 25 D 26 B
13 A 14 A 15 B 16 A 17 C 18 D 19 B 27 c
122 MCQs in Biochemistry for Paramedical Students
28 D 29 A 30 B 31 C 32 D 33 B 34 A 3S B 36 A 37 B 38 C 39 A 40 D 41 c
124 MCQs in Biochemistry for Paramedical Students
5 B 6 c 7 A 8 B 9 D 10 B 11 D 12 A 13 B 14 C 15 D 16 A
128 MCQs in Biochemistry Instrumentation, Techniques and Practicals 129
------ II ----
17. Aromatic amino acids can be qualitatively detected by· 23· pH of normal urine is:
A. Pauly's test · A. 2-3.5
B. Xanthoproteic test B. 4.6-8.0
C. Sulphur test c. 6-8.5
D. Sakaguchi test D . 7-10
18. Urea, uric acid and creatinine are known as: 24. Specific gravity of normal urine is:
A. Pyrimidines A . 1000-1005
B. Amino acid derivatives B. 1010-1015
C. Nonprotein nitrogenous substances c. 1015-1025
D. Polyamines D. 1025-1035
19. Presence of urea can be confirmed by specific urease 25. Name the instrument used to measure specific gravity
test. Which colour develops in this test? of urine:
A. Yellow A. Maclean's Ureameter
B. Red B. pH meter
C. Green C. Urinometer
D. Pink D . Lactometer
20. Which compound is formed in murexide test for uric 26. Low specific gravity in urine is observed in:
acid? A. Diabetes insipidus
A. Ammonium sulphate B. Diabetes mellitus
B. Ammonium purpurate C. Both of the above
C. Ammonium carbonate D. Jaundice
D. Tungsten blue 27. Which one of the following is an inorganic constituents
21. Jaffe' s reaction is a confirmatory test for which substance? present in normal urine?
A. Creatinine A. Albumin
B. Urea B. Glucose
C. Uric acid C. Calcium
D. Peptone D . Bilirubin
22. The test to detect Vitamin A in solution is: 28. An example for organic constituent present in normal
A. Schiff's test urine is:
B. Vanden Berg reaction A. Ammonia
C. Figlu test B. Chloride
D. Cavr Price reaction C. Calcium
D. Urea
17 B 18 C 19 D 20 B 21 A 22 D 23 B 24 C 25 C 26 A 27 C 28 D
130 MCQs in Biochemistry
-
29. Alkaline hypobromite test is used to detect the presence
of which substance in normal urine?
Instrumentation, Techniques and Practicals
29 B 30 D 31 B 32 C 33 D 34 C 35 D 36 D 37 A 38 C 39 D 40 B
132 MCQs in Biochemistry Instrumentation, Techniques and Practicals 133
41. Proteinuria (albuminuria) is observed in: 48. The device used to measure specific gravity of milk is:
A. Kidney diseases A. pH meter
B. Pregnancy B. Glucometer
C. Both of the above C. Lactometer
D. Gout D. Spectrometer
42. Urobilinogen is present in urine in: 49. The white precipitate formed in the quantitative
A. Hemolytic jaundice estimation of urine glucose by Benedict's method of
B. Obstructive jaundice titration is:
C. Hepatic jaundice A. Cuprous oxide
D. All of the above B. Cuprous thiocyante
C. Cupric oxide
43. Spectroscope is used to detect which compounds in
D. Cupric thiocyanate
blood?
A. Osazone crystals 50. Method used for estimation of chlorides in urine is:
B. Cholesterol crystals A. Volhard-Arnold's method
C. Aminoacid derivatives B. Whitehorn-Volhard's method
D. Hemoglobin derivatives C. Both of the above
D. Diacetyl monoxime method
44. Nippe's fluid is used to prepare:
A. Hemin crystals 51. The device used to collect gastric juice is:
B. Osazone crystals A. Endoscope
C. Cholesterol crystals B. Spectroscope
D. None of the above C. Ryle's tube
D. None of the above
45. Which one of the following is a test for cholesterol?
A. Jaffe's reaction 52. The daily output of gastric juice in an normal adult is:
B. Liberman Burchard reaction A. 1-2 lit B. 2-4 lit
C. Acrolein test C. 5-7 lit D. 10-12 lit
D. Uffelman's test 53. pH of gastric juice is:
46. The major sugar present in milk is: A. 1-2 B. 3-5
A. Glucose B. Fructose c. 4.6-8 D. 7-9
C. Maltose D. Lactose 54. Major acid present in gastric juice is:
47. Specific gravity of milk is: A. Acetic acid
A. 1000-1010 B. 1012-1018 B. Lactic acid
C. 1028-1034 D. 1040-2050 C. Carbonic acid
D. Hydrochloric acid
41 C 42 A 43 D 44 A 45 B 46 D 47 C 48 C 49 B 50 A 51 C 52 B 53 A 54 D
134 MCQs in Biochemistry Instrumentation, Techniques and Practicals 135
55. Free acidity of gastric juice is due to: 61. Which instrument is used for quantitative estimation
A. Lactic acid of constituents of body fluids in the clinical laboratory?
B. Hydrochloric acid A. Calorimeter
C. Acetoacetic acid B. pH meter
D. Pyruvic acid C. Colorimeter
D. Centrifuge
56. Total acidity of gastric juice is:
A. 10-15 clinical units 62. Name of the method used to estimate urea in blood is:
B. 10-40 clinical units A. Alkaline picrate method (Jaffe's reaction)
C. 20-55 clinical units B. Biuret method
D. 50-75 clinical units C. Diacetyl monoxirne method
D. Method of caraway
57. The test to detect HCI in gastric juice is:
A. Topfer's test 63. Normal level of blood urea is:
B. Uffleman's test A. 0.5-1 mg/100 ml
C. Benzidine test B. 15-45 mg/100 ml
D. Fouchet's test C. 80-120 mg/100 ml
D. 150-250 mg/100 ml
58. Which of the following is an abnormal constituent in
gastric juice? 64. Electrophoresis is based on the principle of:
A. Starch A. Osmosis
B. Blood B. Dialysis
C. Bile C. Movement of particles between stationary phase and
D. All of the above moving phase
D. Migration of charged particles in an electric field
59. Name of the method used for the estimation of chlorides
in gastric juice is: 65. Paper chromatography is used to separate:
A. Benedict's method A. Proteins
B. Whitehorn-Volhard method B. Amino acids
C. Volhard-Arnold's method C. Nucleic acids
D. None of the above D. Enzymes
60. Normal level of chlorides in gastric juice is:
A. 100-200 mg/100 ml
B. 350-700 mg/100 ml
C. 600-900 mg/100 ml
D. 1-2 g/100 ml
55 B 56 C 57 A 58 D 59 B 60 B 61 C 62 C 63 B 64 D 65 B
Clinical Biochemistry 137
12. In the fasting adult, the sugar in CSF is: 18. Raised plasma protein levels are commonly found in:
A. 15-45 mg/100 ml A. Dehydration ·
B. 45-80 mg/100 ml B. Paraproteinaemias
C. 70-110 mg/100 ml C. Infective hepatitis
D. 80-120 mg/100 ml D. All of the above
13. Blood cholesterol is elevated in which of the following 19. Hypoalbuminemia occurs in:
conditions? A. Tetany
A. Hypothyroidism B. Oedema
B. Hyperthyroidism C. Dehydration
C. Hypoinsulinism D. None of the above
D. None of the above 20. Which of the following serum protein fractions is
14. Estimation of serum triglycerides is of diagno sti c increased in cirrhosis of liver?
importance in: A. y-Globulin
A. Multiple myeloma B. Hyperlipidemias B. ai-Globulin
C. Nephrotic syndrome D. Jaundice C. a 1-Globulin
D. Albumin
15. In Frederickson's classification of the hyperlipo-
proteinemia, type II A is associated with: 21. Which of the changes in serum electrophoretic pattern
A. Decreased LDL Level is diagnostic of nephrotic syndrome?
B. Increased plasma cholesterol level A. Increased albumin
C. Increased triglyceride level B. Increased al-Globulin
D. All of the above C. Increased a2-Globulin
D. Increased y-Globulin
16. Which of the following substances will be responsible
for the characteristic milkiness noted in the plasma in 22. Bence jones proteins may be excreted in urine of patients
postprandial hyperlipemia? suffering from:
A. Chylomicrons A. Diabetic mellitus
B. Cholesterol B. Multiple myeloma
C. Triglycerides C. Diabetes insipidus
D. Free fatty acids D. Hematuria
17. Normal plasma protein levels varies in the range: 23. Immunoglobulin deficiency is observed in:
A. 3.2-5.8 g/10 ml A. Malignant disease
B. 5.0-7.5 g/100 ml B. AIDS
C. 6.0-8.0 g/100 ml C. Newborn infants
D. 7.5-8.5 g/100 ml D. All of the above
12 B 13 A 14 B 15 B 16 A 17 C 18 D 19 B 20 A 21 C 22 B 23 D
140 MCQs in Biochemistry Clinical Biochemistry 141
24. Normal range of protein concentration in CSF is: 30. Tumor marker enzyme in prostatic cancer is:
A. 15-45 mg/100 ml A. Alkaline phosphatase
B. 45-80 mg/100 ml B. Acid phosphatase
C. 3.5-5.5 g/100 ml C. CPK
D. 6.8 g/100 ml D.LDH
25. A very high protein concentration in the CSF together 31. Hypercalcemia is seen in:
with a xanthochromic appearance is characteristic of: A. Malignant disease
A. Purulent meningitis B. Excessive secretion of calcitonin
B. Polyneuritis C. Secondary hyperparathyroidism
C. Encephalitis D. None of the above
D. Cerebral tumour 32. The level of serum phosphate is usually reduced below
26. Hyperuricemia is seen in all the following except: normal in:
A. Polycythemia A. Hypoparathyroidism
B. Xanthinuria B. Tetany
C. Leukemia C. Vitamin D resistant rickets
D. Gout D. All of the above
27. Manifestations of the inherited porphyria include: 33. Plasma iron concentration is typically higher in:
A. Neuropsychiatric symptoms A. Hypochromic microcytic anemia
B. Abdominal Pain B. Rheumatoid arthritis
C. Photosensitivity of the skin C. Hepatitis
D. All of the above D. Acute hemolytic episode
28. Which enzyme is diagnostic in acute pancreatitis? 34. Wilson's disease is characterized by the deposition of
A. Amylase one of the following minerals in the tissues:
B. Acid phosphatase A. Cobalt
C. Alkaline phosphatase B. Copper
D. Crea tine kinase C. Iron
D. Zinc
29. LDHl and LDH2 isoenzymes are significantly elevated
in blood in: 35. The important biochemical abnormalities in Cushing's
A. Nephrotic syndrome syndrome include:
B. Infective hepatitis A. Hypokalemia
C. Myocardial infarction B. Hyponatremia
D. Diabetes mellitus C. Hypoglycemia
D. All of the above
24 A 25 A 26 B 27 D 28 A 29 C 30 B 31 A 32 C 33 D 34 B 35 A
142 MCQs in Biochemistry Clinical Biochemistry 143
36. A marked increase in catecholamine excretion occurs in: 42. Colorimeter is based on:
A. Alkaptonuria A. Beer's law
B. Maple syrup urine disease B. Lambert's law
C. Pheochromocytoma C. Beer Lambert's law
D. Menke' s syndrome D. None of the above
37. Which of the following is an autoimmune disorder? 43. The instrument used to measure sodium and potassium
A. Grave's disease in serum/plasma is:
B. Gilbert's disease A. Ion selective electrodes (ISE)
C. Addison's disease B. Flame photometer
D. Cushing' s syndrome C. Both of the above
38. Normally the 24 hours urinary excretion of creatinine is D. Spectroscope
about: 44. Blood gas analysis (ABG) includes measurement of:
A. 300-700 mg A. p02
B. 1-2 g B. pH and bicarbonate
c. 10-15 g C. pC02
D. 15-dO g D. All of the above
39. Urinary calculi containing calcium may be due to: 45. Which preservative is added to urine to be used for
A. Hypervitaminosis A biochemical analysis?
B. Vitamin D intoxication A. HCl B. Toluene
C. Hyperthyroidism C. Thymol D. All of the above
D. None of the above
40. Which instrument is a dry chemistry auto analyzer?
A. Centrifugal analyzer
B. Discrete analyzer
C. Continuous flow analyzer
D. Kodak Photochem analyzer
41. A sudden rise in serum LDH (380IU/L) and SGOT
(lSOIU/L) and normal liver function tests were observed
in a patient. The probable diagnosis is:
A. Hepatic cirrohosis
B. Myocardial infarction
C. Multiple myeloma
D. Nephrotic syndrome
36 C 37 A 38 B 39 B 40 D 41 B 42 C 43 C 44 D 45 D
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