Sim Medium 2 (English)
Sim Medium 2 (English)
Sim Medium 2 (English)
Dehydrated Product
Difco™ SFP Agar Base Availability
Base Layer: Difco™ SFP Agar Base
AOAC BAM COMPF ISO
1. Suspend 47 g of the powder in 900 mL of purified water.
Cat. No. 281110 Dehydrated – 500 g
Mix thoroughly.
2. Heat with frequent agitation and boil for 1 minute to Difco™ Antimicrobic Vial K
completely dissolve the powder. Cat. No. 233391 Vial – 6 × 10 mL*
3. Autoclave at 121°C for 15 minutes. Cool to 50°C. Difco Antimicrobic Vial P
™
4. Add 100 mL Egg Yolk Enrichment 50%, 10 mL of Cat. No. 232681 Vial – 6 × 10 mL*
rehydrated Antimicrobic Vial P (30,000 units polymyxin Difco Egg Yolk Enrichment 50%
™
SIM Medium
Intended Use Principles of the Procedure
SIM Medium is used to differentiate enteric bacilli on the The ingredients in SIM Medium enable the determination of
basis of sulfide production, indole formation and motility. three activities by which enteric bacteria can be differentiated.
Sodium thiosulfate and ferrous ammonium sulfate are indicators
Summary and Explanation of hydrogen sulfide production. The ferrous ammonium
Hydrogen sulfide production, indole formation and motility sulfate reacts with H2S gas to produce ferrous sulfide, a black
are distinguishing characteristics which aid in the identification precipitate.1 The casein peptone is rich in tryptophan, which is
of the Enterobacteriaceae, especially Salmonella and Shigella. attacked by certain microorganisms resulting in the production
SIM Medium, therefore, is useful in the process of identification of indole. The indole is detected by the addition of chemical
of enteric pathogens.
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SIM Medium, cont.
thoroughly.
2. Heat with frequent agitation and boil for 1 minute to com- Availability
pletely dissolve the powder. BBL™ SIM Medium
BAM
3. Dispense and autoclave at 121°C for 15 minutes. Cat. No. 211578 Dehydrated – 500 g
4. Test samples of the finished product for performance using 221010 Prepared Tubes – Pkg. of 10
stable, typical control cultures. 221011 Prepared Tubes – Ctn. of 100
Procedure
Loosen caps, boil and cool before use. Using growth from
a pure culture, stab an inoculating needle two-thirds of the
distance to the bottom in the center of the tube. Incubate
tubes with loosened caps for 18-24 hours at 35 ± 2°C in an
aerobic atmosphere.
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