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    SOP 001 Polarimeter

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    nitin209
    This standard operating procedure describes the proper use and calibration of a polarimeter made by Rajdhani Scientific Instruments. The analyst must keep the optical elements clean and properly aligned. Several precautions are outlined to ensure accurate measurements, including controlling temperature, avoiding bubbles, and using clear solutions. The procedure describes preparing and inserting the sample solution into the polarimeter tube to obtain a reading, which is then used to calculate the specific optical rotation. The polarimeter is calibrated monthly using sucrose solutions of known concentrations and their expected angle of rotations at 25°C.

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    SOP 001 Polarimeter

    Uploaded by

    nitin209
    4K views2 pages
    This standard operating procedure describes the proper use and calibration of a polarimeter made by Rajdhani Scientific Instruments. The analyst must keep the optical elements clean and properly aligned. Several precautions are outlined to ensure accurate measurements, including controlling temperature, avoiding bubbles, and using clear solutions. The procedure describes preparing and inserting the sample solution into the polarimeter tube to obtain a reading, which is then used to calculate the specific optical rotation. The polarimeter is calibrated monthly using sucrose solutions of known concentrations and their expected angle of rotations at 25°C.

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    This standard operating procedure describes the proper use and calibration of a polarimeter made by Rajdhani Scientific Instruments. The analyst must keep the optical elements clean and properly aligned. Several precautions are outlined to ensure accurate measurements, including controlling temperature, avoiding bubbles, and using clear solutions. The procedure describes preparing and inserting the sample solution into the polarimeter tube to obtain a reading, which is then used to calculate the specific optical rotation. The polarimeter is calibrated monthly using sucrose solutions of known concentrations and their expected angle of rotations at 25°C.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    4K views2 pages

    SOP 001 Polarimeter

    Uploaded by

    nitin209
    This standard operating procedure describes the proper use and calibration of a polarimeter made by Rajdhani Scientific Instruments. The analyst must keep the optical elements clean and properly aligned. Several precautions are outlined to ensure accurate measurements, including controlling temperature, avoiding bubbles, and using clear solutions. The procedure describes preparing and inserting the sample solution into the polarimeter tube to obtain a reading, which is then used to calculate the specific optical rotation. The polarimeter is calibrated monthly using sucrose solutions of known concentrations and their expected angle of rotations at 25°C.

    Copyright:

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    Download as DOC, PDF, TXT or read online from Scribd
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    of 2

    CHARAK CONFIDENTIAL

    STANDARD OPERATING PROCEDURE


    Name of the Instrument: Polarimeter (Make: Rajdhani Scientific Instruments)
    S.O.P. No.: ADI/SAM-001 Issue No.: 4 Page No.: 1 of 2
    Supercedes: Issue No.3, dated
    Date of Issue: March 2006 Date of Review: March 2009
    November 2003
    1.0 Purpose: This SOP describes the procedure for operating Polarimeter. (Make: Rajdhani Scientific
    Instruments)

    2.0 Responsibility: Analyst

    3.0 Precautions:
     Optical elements must be kept clean and in exact alignment. The match point should be close
    to the normal zero mark.
     The field must be evenly illuminated and it should be supplemented by a filtering system
    capable of isolating the D line from sodium light.
     Specific attention should be paid to temperature control of the solution and of the polarimeter.
     Polarimeter tubes should be filled in such a way so as to avoid air bubbles. Particular care is
    necessary for semi-micro and micro tubes.
     For tubes with removable end plates fitted with gaskets and caps, tighten the end plates only
    enough to ensure leak proof seal between the end plate and body of the tube.
     Liquids and solutions of solids must be clear.
     Differences between the observed and corrected optical rotation calculated as either specific
    optical rotation or optical rotation should not be more than ¼th of the range specified in the
    monograph of the substance.

    4.0 Procedure:
     Sample solution as per monograph is prepared and filled in Polarimeter tube.
     The tube is inserted in the slot provided ensuring that there is no air bubble in the path of the
    light and the analyzer is rotated until two halves of field shows equal intensity of light. Adjust
    the reading with fine adjustment knob such that the cross wires exactly coincide with one of
    the readings on scale.
    Reading (optical rotation) x 100
    Specific optical rotation = ----------------------------------------------
    L x C
    (where, L = length of tube, C = Concentration of the substance in percentage w/v)

    Prepared By Approved By Issued By

    (Ms. Sabina Golatkar) (Mr. Hemant Patil) (Mr. P. Gopinath)


    (Senior Executive- Q. A.) (Sr. Manager - Corporate Q.A) (Gen. Manager-Tech. Services)
    CHARAK CONFIDENTIAL

    STANDARD OPERATING PROCEDURE


    Name of the Instrument: Polarimeter
    S.O.P. No: ADI/SAM-001 (Make: Rajdhani Scientific Page No: 2 of 2
    Instruments)
    5.0 Calibration:
    The polarimeter can be calibrated by measuring the optical rotation in a 2 dm table at 25° using
    the concentrations of dry sucrose indicated in the table below

    Concentration g/ 100 ml Angle of rotation (+) at 25°


    10.0 13.33
    20.0 26.61
    30.0 39.86
    40.0 53.06
    50.0 66.23

    6.0 Frequency of calibration: Monthly

    HISTORY OF CHANGES
    Issue No. Date Remark
    1. March 2000 Original Issue
    2. November 2001 Format revised
    3. November 2003 Reviewed & issued without any change
    4. March 2006 Reviewed & issued without any change

    Prepared By Approved By Issued By

    (Ms. Sabina Golatkar) (Mr. Hemant Patil) (Mr. P. Gopinath)


    (Senior Executive- Q. A.) (Sr. Manager - Corporate Q.A) (Gen. Manager-Tech. Services)

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