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    Methods of Preparing Blood Smear

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    Kriziaoumo P. Orpia
    This document provides information on methods for preparing blood smears, types of stains used in differential counting, methods of staining, and methods of differential staining. It also includes descriptions of common blood cells including their nuclei, cytoplasm, and normal values. Finally, it lists the recommended order of draw for phlebotomy and the number of inversions and additives for different evacuated tube systems.

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    Methods of Preparing Blood Smear

    Uploaded by

    Kriziaoumo P. Orpia
    5K views2 pages
    This document provides information on methods for preparing blood smears, types of stains used in differential counting, methods of staining, and methods of differential staining. It also includes descriptions of common blood cells including their nuclei, cytoplasm, and normal values. Finally, it lists the recommended order of draw for phlebotomy and the number of inversions and additives for different evacuated tube systems.

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    Hematology Laboratory

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    This document provides information on methods for preparing blood smears, types of stains used in differential counting, methods of staining, and methods of differential staining. It also includes descriptions of common blood cells including their nuclei, cytoplasm, and normal values. Finally, it lists the recommended order of draw for phlebotomy and the number of inversions and additives for different evacuated tube systems.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    5K views2 pages

    Methods of Preparing Blood Smear

    Uploaded by

    Kriziaoumo P. Orpia
    This document provides information on methods for preparing blood smears, types of stains used in differential counting, methods of staining, and methods of differential staining. It also includes descriptions of common blood cells including their nuclei, cytoplasm, and normal values. Finally, it lists the recommended order of draw for phlebotomy and the number of inversions and additives for different evacuated tube systems.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    Methods of Preparing Blood Smear

    1. Two Slide or Wedge Method


    2. Two Cover Slip Method or Erlich Method
    3. Cover Glassand Slide Method or Spinner’s Method
    Types of Stains Used in Differential Counting
    1. Romanowsky Stains
    a. Wright Stain
    b. Giemsa Stain
    c. Leishman Stain
    2. Panoptic Stains
    a. Jenner- Giemsa Stain
    b. May- Grunwald- Griemsa Stain
    Methods of Staining
    1. Staining Dish Method
    2. Staining Jar or DIP Method
    3. Automatic Method
    Methods of Differential Staining
    1. Four –Field Meander Method
    2. Two Field Method
    3. Exaggerated Battered Method
    4. Strip Differential Method

    Nucleus Cytoplasm Normal Value


    Neutrophils Broken into segment Small pinkish stains 60-70%
    Or 55-70%
    Lymphocyte Closely knitted; usually Light Blue 25-35% up to 40%
    round
    a)Large Immature and found
    rarely; Usually mistaken as
    Monocytes
    b)Small Mature, typical
    Neutrophil BANDS Younger form of 2-6%
    neutrophils with a C, S or
    horse-shoe shape
    appearance
    Eosinophils Has 2 lobes a large coarse 1-4%
    reddish or orange
    granules
    Monocyte- Large cell in Spongy. Sprawling with Light gray 2-6%
    the circulation brain- like coagulation
    Basophils Indistinct and appear Purplish black or bluish 0-1%
    burned under large Granules

    Criteria of a Good Blood Smear


    1. The thick area makes a gradual transition to the thin area (feather-like edge)
    2. The blood in the thin area does not extend to the end of the side
    3. Must have smooth and even surface
    4. Leukocytes must be clumping
    RECOMMENDED ORDER OF DRAW FOR PHLEBOTOMY

    Evacuated Tube System No. of Inversion Additive


    Light Blue 3-4 Sodium Citrate
    Yellow 8 ACD (Acid Citrate Dextrose)
    Gold 5 SST (Serum Separating Tube)
    Red(Glass) 0 None
    Red (plastic) 5 Clot activator
    Light Green 8 Plasma Separating Tube
    Green 8 Sodium Heparin
    Purple 8 K2EDTA; K3EDTA
    Gray 8 Potassium Oxalate

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