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    Lab Report DNA Extraction

    Uploaded by

    Ezio Auditore
    Nanodrop spectrophotometry can measure very concentrated samples without dilution, up to 50 times more concentrated than standard cuvette-based methods, since it uses surface tension instead of cuvettes to hold samples. Extracting genomic DNA from cheek cells is easier than extracting plasmid DNA since it only requires breaking open cells through lysis to release the whole genome, while extracting plasmids requires separating them from genomic DNA. Culturing bacteria for 16 hours is optimal for extracting plasmid DNA since it is when the culture transitions from logarithmic to stationary phase, resulting in a higher ratio of plasmid DNA to RNA compared to logarithmic phase, but before DNA degradation occurs in later stationary phase.

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    Lab Report DNA Extraction

    Uploaded by

    Ezio Auditore
    2K views1 page
    Nanodrop spectrophotometry can measure very concentrated samples without dilution, up to 50 times more concentrated than standard cuvette-based methods, since it uses surface tension instead of cuvettes to hold samples. Extracting genomic DNA from cheek cells is easier than extracting plasmid DNA since it only requires breaking open cells through lysis to release the whole genome, while extracting plasmids requires separating them from genomic DNA. Culturing bacteria for 16 hours is optimal for extracting plasmid DNA since it is when the culture transitions from logarithmic to stationary phase, resulting in a higher ratio of plasmid DNA to RNA compared to logarithmic phase, but before DNA degradation occurs in later stationary phase.

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    lab report DNA extraction

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    lab report DNA extraction

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    Nanodrop spectrophotometry can measure very concentrated samples without dilution, up to 50 times more concentrated than standard cuvette-based methods, since it uses surface tension instead of cuvettes to hold samples. Extracting genomic DNA from cheek cells is easier than extracting plasmid DNA since it only requires breaking open cells through lysis to release the whole genome, while extracting plasmids requires separating them from genomic DNA. Culturing bacteria for 16 hours is optimal for extracting plasmid DNA since it is when the culture transitions from logarithmic to stationary phase, resulting in a higher ratio of plasmid DNA to RNA compared to logarithmic phase, but before DNA degradation occurs in later stationary phase.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    2K views1 page

    Lab Report DNA Extraction

    Uploaded by

    Ezio Auditore
    Nanodrop spectrophotometry can measure very concentrated samples without dilution, up to 50 times more concentrated than standard cuvette-based methods, since it uses surface tension instead of cuvettes to hold samples. Extracting genomic DNA from cheek cells is easier than extracting plasmid DNA since it only requires breaking open cells through lysis to release the whole genome, while extracting plasmids requires separating them from genomic DNA. Culturing bacteria for 16 hours is optimal for extracting plasmid DNA since it is when the culture transitions from logarithmic to stationary phase, resulting in a higher ratio of plasmid DNA to RNA compared to logarithmic phase, but before DNA degradation occurs in later stationary phase.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
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    Discussion

    Advantages of Nanodrop over cuvette based spectrophotometry is that


    it is capable of measuring very concentrated samples without dilution
    it is 50 times higher than measurements by standard cuvette based
    spectrophotometry. Also it has a sample retention technology that uses
    only surface tension to hold the sample thus there is no need in
    cuvettes.
    Cheek cell DNA extraction is the extraction of the genomic DNA;
    therefore, it is easier to isolate the whole genomic DNA because it is
    just needed a good lysis step to release genomic DNA. Whereas,
    extracting plasmid DNA is a bit more complicatd because it requires
    separating plasmid from the genomic DNA. In addition, in isolating
    genomic DNA, chewing the cheeks was the primary method of cell lysis
    (physical) whereas in plasmid extraction SDS-alkaline method was
    applied for the lysis.
    Culturing bacteria for 16 hours is optimal as it is the transition from
    logarithmic into stationary growth phase. At this time, the ratio of
    plasmid DNA to RNA is higher than during the logarithmic phase. If
    culturing last longer, DNA might be degraded due to over aging of the
    culture in the later stationary phase.

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