7 - Cytology of Body Fluid
7 - Cytology of Body Fluid
7 - Cytology of Body Fluid
Feature
Gross appearance Specific gravity
Transudate
Watery, clear
Exudate
Turbid or cloudy
Protein
Clots cells
No
Usually benign: Few
Yes
More mesothelial cells, or chronic
mesothelial acute
cells,
Tract
Tract
Cavity Tract
Gastrointestinal Effusions
Cerebral
Spinal Fluid
fluid
Amniotic
exam
Microscopic Any
cytology(
Test
SAMPLE COLLECTION
FIXATION
1ml
clotting
N.B.:
Stain
Hx
&E
block for remnant sediment and histopathological
Cell
examination.
Other
confirm diagnosis.
Cell block
Adding plasma and thrombin solution Wrapped in filter paper Placed in a cassette
Embedded in paraffin
Cut and H&E stain
1- CEREBROSPINAL FLUID
Fluid
count
and differentiate cells seen usually are few; increased with viral,
Lymphocytes:
Eosinophils/Basophils:
Plasma cells: not normally present; associated with viral disorders, and Hodgkin's diseases.
Effusion:
Transudate
Exudates
Cells unique to the lungs: Mesothelial cells RBCs and WBCs: are limited, if increased without
3- PERITONEAL FLUID
Abnormal
cavity: Ascites
Ascites:
Must have an accumulation of > 100ml (several 100) before effusion can be detected on physical exam.
Removal procedure-
paracentesis
PHYSICAL CHARACTERISTICS
Peritoneal
Total
4- Pericardial Fluid
Pericardial
Measure pH: pH less than 7.0 associated with infection or rheumatoid disorder.
5- Seminal Fluid
Examine physical, chemical and microscopic detail Count number of sperm, report morphology and motility
6- Synovial Fluid:
Viscosity test Mucin Clot test Note crystals (intracellular vs. extra cellular)
Slide exam: usually performed on concentration of the fluid using Giemsa or Papnicolaou
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