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Search Results (1,884)

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15 pages, 7414 KiB  
Article
Automated Fixed System Specifically Designed for Agrochemical Applications in Protected Crops
by Souraya Benalia, Antonio Mantella, Matteo Sbaglia, Lorenzo M. M. Abenavoli and Bruno Bernardi
Agriculture 2025, 15(3), 330; https://doi.org/10.3390/agriculture15030330 (registering DOI) - 2 Feb 2025
Viewed by 85
Abstract
Protected crops are intensive production systems characterized by high vegetation density, high temperatures, and high moisture, making them favorable environments for the development of pests and diseases. Consequently, these systems often require several interventions with agrochemicals to maintain profitable yields and high produce [...] Read more.
Protected crops are intensive production systems characterized by high vegetation density, high temperatures, and high moisture, making them favorable environments for the development of pests and diseases. Consequently, these systems often require several interventions with agrochemicals to maintain profitable yields and high produce quality. However, the application of plant protection products (PPPs) in such systems is not efficient and poses environmental concerns. This study aims at analysing spray behaviour, particularly in terms of foliar deposition and losses to the ground according to spraying equipment and foliage height, focusing on a specifically designed and developed system for agrochemical application in protected crops, and comparing it with a commonly used spraying system, namely, the cannon sprayer. Such a system consists in a fixed net of tubing and anti-drip nozzles positioned at the top of the greenhouse’s apex, connected to a pneumatic sprayer ‘Special Serre 2000’ outside the greenhouse. Findings revealed a significant effect of the spraying system (Kruskal–Wallis χ2 = 12.239, df = 1, and p-value = 0.0004681) on normalized foliar deposition, with higher values obtained using the fixed spraying system. In addition, a simulation of the spatial distribution based on the principle of inverse distance weighting (IDW) was performed for qualitative spray assessment, confirming the heterogeneity of foliar deposition over the greenhouse with both of the used equipment. In addition, losses to the ground were affected by both spraying equipment and captor position. Full article
(This article belongs to the Section Crop Protection, Diseases, Pests and Weeds)
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Figure 1

Figure 1
<p>Representative scheme of the fixed net of tubes and nozzles of the Special Serre 2000 system inside the greenhouse. Numbers in the figure indicate the distances expressed in metre (m). Particularly: greenhouse span width W = 9 m; greenhouse span length L = 45 m; distance between pepper rows = 1.3 m; distance between pepper plants in the row = 0.4 m; layout of the net of nuzzles = 3 m × 3 m; distance between the edge of the greenhouse span and the first tubing line = 1.1 m; height of the fixed net of tubing and anti-drip nozzles NH = 2.5 m.</p>
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<p>The first spraying system ‘Special Serre 2000’ used in experimental trials on a protected pepper crop.</p>
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<p>The second spraying system ‘Tifone Storm 2000 80S model’ used in experimental trials on a protected pepper crop.</p>
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<p>Green pepper crop considered for experimental trials.</p>
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<p>Application of the tartrazine yellow (E102 85%) solution on protected pepper crop using the two spraying systems.</p>
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<p>Natural target (leaves) sampling points at plant level (in red).</p>
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<p>Calibration curve used for spectrophotometer calibration and retrieval of E102 concentration intercepted by targets.</p>
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<p>Normalized foliar deposition mean values (±Dev. St.) expressed in µL·cm<sup>−2</sup>.</p>
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<p>Normalized foliar deposition mean values (±Dev. St.) expressed in µL·cm<sup>−2</sup> in function of foliage sampling height for each spraying system.</p>
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<p>Interpolation of spray deposition over the upper foliage level. (<b>Left</b>): SS2000, dots represent nozzles position; (<b>Right</b>): cannon.</p>
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<p>Interpolation of spray deposition over the lower foliage level. (<b>Left</b>): SS2000, dots represent nozzles position; (<b>Right</b>): cannon.</p>
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<p>Normalized losses to the ground mean values (±Dev. St.) expressed in µL·cm<sup>−2</sup>.</p>
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<p>Normalized losses to the ground mean values (±Dev. St.) expressed in µL·cm<sup>−2</sup> according to sampling position for each spraying system. BR: between rows; UV: under vegetation.</p>
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19 pages, 15140 KiB  
Article
Evaluation of Impact of Soil on Performance of Monopole Antenna for IoT Applications in Urban Agriculture
by Nikolay Todorov Atanasov, Blagovest Nikolaev Atanasov and Gabriela Lachezarova Atanasova
Electronics 2025, 14(3), 544; https://doi.org/10.3390/electronics14030544 - 29 Jan 2025
Viewed by 363
Abstract
Built indoor IoT-based urban farms successfully combine the cultivation of fresh vegetables with attractive architectural designs. Moreover, implementing IoT-driven urban agriculture requires installing multiple IoT devices containing sensors, controllers, transceivers, and antennas for real-time data transmission. In this context, several factors, including the [...] Read more.
Built indoor IoT-based urban farms successfully combine the cultivation of fresh vegetables with attractive architectural designs. Moreover, implementing IoT-driven urban agriculture requires installing multiple IoT devices containing sensors, controllers, transceivers, and antennas for real-time data transmission. In this context, several factors, including the height of the IoT device above the soil level and the water content in the soil, can affect antenna performance and, consequently, the propagation of radio waves. This paper presents the results from numerical and experimental studies that evaluate the impact of soil on the performance of a monopole antenna for three different antenna positions relative to the soil in a pot and two soil water contents, presented by twelve scenarios. The results show that the antenna has a stable performance in six of the twelve scenarios, with a minimal shift in the resonant frequency of 3% and a narrowing of the frequency bandwidth by 2% compared to the antenna in free space. In the worst-case scenario, the antennas demonstrate a reduction in radiation efficiency of 44%, with the frequency bandwidth narrowing by up to 14% for the antenna fabricated on a PLA substrate and up to 17% for the one built on a foam board substrate. Full article
(This article belongs to the Special Issue Antennas for IoT Devices)
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<p>Diagram of soil structure: (<b>a</b>) oven-dried soil; (<b>b</b>) permanent wilting point; (<b>c</b>) saturation; and (<b>d</b>) field capacity. The blue color represents water, white represents air, and brown represents soil particles.</p>
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<p>Numerical models: (<b>a</b>) antenna structure designed with PLA substrate; (<b>b</b>) antenna structure designed with foam board substrate; and (<b>c</b>) plastic pot filled with soil.</p>
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<p>Setup for numerical simulations: (<b>a</b>) Scenarios 1–3 for antennas with PLA and (<b>b</b>) Scenarios 1–3 for antennas with foam board substrates.</p>
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<p>Magnitudes of simulated reflection coefficients (|S<sub>11</sub>|) of the antenna with a PLA substrate located at the center of the pot for three different antenna positions relative to the soil at 45% soil moisture.</p>
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<p>Magnitudes of simulated reflection coefficients (|S<sub>11</sub>|) of the antenna with a PLA substrate located at the center of the pot for three different antenna positions relative to the soil at 10% soil moisture.</p>
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<p>Magnitudes of simulated reflection coefficients (|S<sub>11</sub>|) of the antenna with a foam board substrate located at the center of the pot for three different antenna positions relative to the soil at 45% soil moisture.</p>
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<p>Magnitudes of simulated reflection coefficients (|S<sub>11</sub>|) of the antenna with a foam board substrate located at the center of the pot for three different antenna positions relative to the soil at 10% soil moisture.</p>
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<p>Setup for numerical simulations: (<b>a</b>) Scenarios 7–9 for antennas with PLA and (<b>b</b>) Scenarios 7–9 for antennas with foam board substrates.</p>
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<p>Magnitudes of simulated reflection coefficients (|S<sub>11</sub>|) of the antenna with a PLA substrate located at the edge of a pot for three different antenna positions relative to the soil at 45% soil moisture.</p>
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<p>Magnitudes of simulated reflection coefficients (|S<sub>11</sub>|) of the antenna with a PLA substrate located at the edge of a pot for three different antenna positions relative to the soil at 10% soil moisture.</p>
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<p>Magnitudes of simulated reflection coefficients (|S<sub>11</sub>|) of the antenna with a foam board substrate located at the edge of a pot for three different antenna positions relative to the soil at 45% soil moisture.</p>
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<p>Magnitudes of simulated reflection coefficients (|S<sub>11</sub>|) of the antenna with a foam board substrate located at the edge of a pot for three different antenna positions relative to the soil at 10% soil moisture.</p>
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<p>Three-dimensional radiation patterns at 2.5 GHz: (<b>a</b>) antenna on a PLA substrate—in the free space front view; (<b>b</b>) antenna on a PLA substrate at H = 70 mm at the center of the pot; (<b>c</b>) antenna on a PLA substrate at H = 0 mm at the center of the pot; (<b>d</b>) antenna on a foam board substrate—in the free space front view; (<b>e</b>) antenna on a foam board substrate at H = 70 mm; (<b>f</b>) antenna on a foam board substrate at H = 0 mm at the center of the pot; (<b>g</b>) antenna on a PLA substrate—in the free space—side view; (<b>h</b>) antenna on a PLA substrate at H = 70 mm at the edge of the pot; (<b>i</b>) antenna on a PLA substrate at H = 0 mm at the edge of the pot; (<b>j</b>) antenna on a foam board substrate—in the free space—side view; (<b>k</b>) antenna on a foam board substrate at H = 70 mm at the edge of the pot; and (<b>l</b>) antenna on a foam board substrate at H = 0 mm at the edge of the pot.</p>
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<p>Three-dimensional radiation patterns at 2.5 GHz: (<b>a</b>) antenna on a PLA substrate—in the free space front view; (<b>b</b>) antenna on a PLA substrate at H = 70 mm at the center of the pot; (<b>c</b>) antenna on a PLA substrate at H = 0 mm at the center of the pot; (<b>d</b>) antenna on a foam board substrate—in the free space front view; (<b>e</b>) antenna on a foam board substrate at H = 70 mm; (<b>f</b>) antenna on a foam board substrate at H = 0 mm at the center of the pot; (<b>g</b>) antenna on a PLA substrate—in the free space—side view; (<b>h</b>) antenna on a PLA substrate at H = 70 mm at the edge of the pot; (<b>i</b>) antenna on a PLA substrate at H = 0 mm at the edge of the pot; (<b>j</b>) antenna on a foam board substrate—in the free space—side view; (<b>k</b>) antenna on a foam board substrate at H = 70 mm at the edge of the pot; and (<b>l</b>) antenna on a foam board substrate at H = 0 mm at the edge of the pot.</p>
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<p>Radiation efficiency of the antennas in the frequency range of 1.83 GHz to 4.2 GHz.</p>
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<p>Photos: (<b>a</b>) antenna prototypes—with a PLA substrate (left) and foam board substrate (right); (<b>b</b>) plastic pots filled with soil with a moisture content of 10% (left) and filled with soil that had reached saturation (30% soil moisture), where all soil pores were filled with water (right); (<b>c</b>) Scenarios 1–3 for antennas with PLA; (<b>d</b>) Scenarios 4–6 for antennas with foam board substrate; (<b>e</b>) Scenarios 7–9 for antennas with foam board substrate; and (<b>f</b>) Scenarios 10–12 for antennas with PLA substrate.</p>
Full article ">Figure 15 Cont.
<p>Photos: (<b>a</b>) antenna prototypes—with a PLA substrate (left) and foam board substrate (right); (<b>b</b>) plastic pots filled with soil with a moisture content of 10% (left) and filled with soil that had reached saturation (30% soil moisture), where all soil pores were filled with water (right); (<b>c</b>) Scenarios 1–3 for antennas with PLA; (<b>d</b>) Scenarios 4–6 for antennas with foam board substrate; (<b>e</b>) Scenarios 7–9 for antennas with foam board substrate; and (<b>f</b>) Scenarios 10–12 for antennas with PLA substrate.</p>
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<p>Magnitudes of measured reflection coefficients (|S<sub>11</sub>|) of the antenna with a PLA substrate located at the center of the pot for three different antenna positions relative to the soil in the pot at 30% soil moisture.</p>
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<p>Magnitudes of measured reflection coefficients (|S<sub>11</sub>|) of the antenna with a PLA substrate located at the center of the pot for three different antenna positions relative to the soil in the pot at 10% soil moisture.</p>
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<p>Magnitudes of measured reflection coefficients (|S<sub>11</sub>|) of the antenna with a foam board substrate located at the center of the pot for three different antenna positions relative to the soil in the pot at 30% soil moisture.</p>
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<p>Magnitudes of measured reflection coefficients (|S<sub>11</sub>|) of the antenna with a foam board substrate located at the center of the pot for three different antenna positions relative to the soil in the pot at 10% soil moisture.</p>
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<p>Magnitudes of measured reflection coefficients (|S<sub>11</sub>|) of the antenna with a PLA substrate located at the edge of the pot for three different antenna positions relative to the soil in the pot at 30% soil moisture.</p>
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<p>Magnitudes of measured reflection coefficients (|S<sub>11</sub>|) of the antenna with a PLA substrate located at the edge of the pot for three different antenna positions relative to the soil in the pot at 10% soil moisture.</p>
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<p>Magnitudes of measured reflection coefficients (|S<sub>11</sub>|) of the antenna with a foam board substrate located at the edge of the pot for three different antenna positions relative to the soil in the pot at 30% soil moisture.</p>
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<p>Magnitudes of measured reflection coefficients (|S<sub>11</sub>|) of the antenna with a foam board substrate located at the edge of the pot for three different antenna positions relative to the soil in the pot at 10% soil moisture.</p>
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29 pages, 6516 KiB  
Article
Remote Sensing-Assisted Estimation of Water Use in Apple Orchards with Permanent Living Mulch
by Susana Ferreira, Juan Manuel Sánchez, José Manuel Gonçalves, Rui Eugénio and Henrique Damásio
Agronomy 2025, 15(2), 338; https://doi.org/10.3390/agronomy15020338 - 28 Jan 2025
Viewed by 564
Abstract
Orchards are complex agricultural systems with various characteristics that influence crop evapotranspiration (ETc), such as variety, tree height, planting density, irrigation methods, and inter-row management. The preservation of biodiversity and improvement of soil fertility have become important goals in modern orchard [...] Read more.
Orchards are complex agricultural systems with various characteristics that influence crop evapotranspiration (ETc), such as variety, tree height, planting density, irrigation methods, and inter-row management. The preservation of biodiversity and improvement of soil fertility have become important goals in modern orchard management. Consequently, the traditional approach to weed control between rows, which relies on herbicides and soil mobilization, has gradually been replaced by the use of permanent living mulch (LM). This study explored the potential of a remote sensing (RS)-assisted method to monitor water use and water productivity in apple orchards with permanent mulch. The experimental data were obtained in the Lis Valley Irrigation District, on the Central Coast of Portugal, where the “Maçã de Alcobaça” (Alcobaça apple) is produced. The methodology was applied over three growing seasons (2019–2021), combining ground observations with RS tools, including drone flights and satellite images. The estimation of ETa followed a modified version of the Food and Agriculture Organization of the United Nations (FAO) single crop coefficient approach, in which the crop coefficient (Kc) was derived from the normalized difference vegetation index (NDVI) calculated from satellite images and incorporated into a daily soil water balance. The average seasonal ETa (FAO-56) was 824 ± 14 mm, and the water productivity (WP) was 3.99 ± 0.7 kg m−3. Good correlations were found between the Kc’s proposed by FAO and the NDVI evolution in the experimental plot, with an R2 of 0.75 for the entire growing season. The results from the derived RS-assisted method were compared to the ETa values obtained from the Mapping Evapotranspiration at High Resolution with Internalized Calibration (METRIC) surface energy balance model, showing a root mean square (RMSE) of ±0.3 mm day−1 and a low bias of 0.6 mm day−1. This study provided insights into mulch management, including cutting intensity, and its role in maintaining the health of the main crop. RS data can be used in this management to adjust cutting schedules, determine Kc, and monitor canopy management practices such as pruning, health monitoring, and irrigation warnings. Full article
(This article belongs to the Section Water Use and Irrigation)
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<p>Geographic location of the LVID. The red line marks the LVID boundary, the blue line traces the course of the Lis River, and the orange lines outline the plot boundaries. The green area in the lower left corner represents the layout of the “Vitor Duarte Experimental Plot” (source: Google Earth, <a href="https://earth.google.com" target="_blank">https://earth.google.com</a>, accessed on 12 September 2024).</p>
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<p>Average monthly air temperature and precipitation on the LVID (P—precipitation; T<sub>min</sub>—minimum air temperature; T<sub>mean</sub>—mean air temperature; T<sub>max</sub>—maximum air temperature) (adapted from [<a href="#B65-agronomy-15-00338" class="html-bibr">65</a>]).</p>
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<p>Permanent mulching in the orchard, represented by the herbs growing between the rows of apple trees (photo taken on 12 June 2024).</p>
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<p>Drone used for the data collection flight (<b>a</b>), measurement of soil moisture with a portable probe (<b>b</b>), automatic agrometeorological station (<b>c</b>), and a GreenSeeker Handheld Crop Sensor being used in a maize field (<b>d</b>), illustrating the methodology employed for NDVI measurements in this study. Although the image was taken in a maize field, the same approach was applied to the apple orchards.</p>
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<p>Groundwater table depth (h: depth below ground) measured during the 2019 and 2020 apple growing seasons.</p>
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<p>Average volumetric soil moisture content (% by volume) at depths of 20, 30, and 50 cm during the 2019 and 2020 growing seasons. Error bars indicate the variability in the measurements, represented by the standard deviation.</p>
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<p>Observed total available water content (AWC) and available water storage (AWS) of the soil during the 2019 and 2020 seasons.</p>
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<p>Crop coefficient curve for apple orchards with permanent mulching, showing the initial stage (Kc<sub>ini</sub>), mid-season stage (Kc<sub>mid</sub>), and end of the late season stage (Kc<sub>end</sub>). The color bars represent the stages of the growing period.</p>
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<p>Daily ET<sub>o</sub> and daily ET<sub>a</sub> values calculated during the apple growing seasons of 2019 (<b>a</b>), 2020 (<b>b</b>), and 2021 (<b>c</b>) at the study site. Rainfall is represented by vertical bars.</p>
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<p>Daily ET<sub>o</sub> and daily ET<sub>a</sub> values calculated during the apple growing seasons of 2019 (<b>a</b>), 2020 (<b>b</b>), and 2021 (<b>c</b>) at the study site. Rainfall is represented by vertical bars.</p>
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<p>NDVI extracted from SPIDER (dotted lines for 2019, 2020, and 2021), ground field measurements (blue dots for 2020), and METRIC EEFlux (red squares for 2019, 2020, and 2021). Error bars represent the standard deviation on the 3 × 3-pixel averages from METRIC. Yellow circles represent the cutting events for the living mulch, highlighting the timing of each intervention.</p>
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<p>The NDVI processed from the drone flight conducted on 26 July 2021.</p>
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<p>ET<sub>a</sub> from RS-assisted FAO-56 evolution compared with ET<sub>a</sub> from the traditional FAO-56 method during the apple growing seasons of 2019 (<b>a</b>), 2020 (<b>b</b>), and 2021 (<b>c</b>) at the study site.</p>
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<p>ET<sub>a</sub> from RS-assisted FAO-56 evolution compared with ET<sub>a</sub> from the traditional FAO-56 method during the apple growing seasons of 2019 (<b>a</b>), 2020 (<b>b</b>), and 2021 (<b>c</b>) at the study site.</p>
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<p>Examples of ET<sub>a</sub> maps obtained from the METRIC (<b>left</b>) and the RS-assisted FAO-56 approach (<b>right</b>) for two different dates: 24 August 2019 (<b>upper</b>) and 6 May 2020 (<b>lower</b>). The apple orchard is outlined in green.</p>
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<p>Linear regression between mean ET<sub>a</sub> values provided by METRIC and ET<sub>a</sub> calculated from the derived K<sub>c</sub> = K<sub>c</sub>(NDVI) relationship. The dotted line represents the linear regression. The gray line represents the 1:1 relationship, included as a reference to evaluate the agreement between observed and estimated values. Error bars indicate spatial variability, representing the standard deviation of ET<sub>a</sub> values from METRIC on the <span class="html-italic">x</span>-axis and ET<sub>a</sub> resulting from the K<sub>c</sub> = K<sub>c</sub>(NDVI) approach on the <span class="html-italic">y</span>-axis.</p>
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23 pages, 9644 KiB  
Article
Modeling Urban Microclimates for High-Resolution Prediction of Land Surface Temperature Using Statistical Models and Surface Characteristics
by Md Golam Rabbani Fahad, Maryam Karimi, Rouzbeh Nazari and Mohammad Reza Nikoo
Urban Sci. 2025, 9(2), 28; https://doi.org/10.3390/urbansci9020028 - 28 Jan 2025
Viewed by 534
Abstract
Surface properties in complex urban environments can significantly impact local-level temperature gradients and distribution on several scales. Studying temperature anomalies and identifying heat pockets in urban settings is challenging. Limited high-resolution datasets are available that do not translate into an accurate assessment of [...] Read more.
Surface properties in complex urban environments can significantly impact local-level temperature gradients and distribution on several scales. Studying temperature anomalies and identifying heat pockets in urban settings is challenging. Limited high-resolution datasets are available that do not translate into an accurate assessment of near-surface temperature. This study developed a model to predict land surface temperature (LST) at a high spatial–temporal resolution in urban areas using Landsat data and meteorological inputs from NLDAS. This study developed an urban microclimate (UC) model to predict air temperature at high spatial–temporal resolution for inner urban areas through a land surface and build-up scheme. The innovative aspect of the model is the inclusion of micro-features in land use characteristics, which incorporate surface types, urban vegetation, building density and heights, short wave radiation, and relative humidity. Statistical models, including the Generalized Additive Model (GAM) and spatial autoregression (SAR), were developed to predict land surface temperature (LST) based on surface characteristics and weather parameters. The model was applied to urban microclimates in densely populated regions, focusing on Manhattan and New York City. The results indicated that the SAR model performed better (R2 = 0.85, RMSE = 0.736) in predicting micro-scale LST variations compared to the GAM (R2 = 0.39, RMSE = 1.203) and validated the accuracy of the LST prediction model with R2 ranging from 0.79 to 0.95. Full article
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<p>Administrative boundary of the borough of Manhattan in NYC and dominant land use types based on the latest National Land Cover Database (NLCD 2019).</p>
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<p>Key steps, required data processing, and methods for the proposed urban meteorological model.</p>
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<p>Snapshot of converted land surface temperature (°C) of New York City mapped within the zip codes.</p>
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<p>Comparison of downscaled NLDAS temperature with observed weather station data within three buffer zones (i.e., 1 km, 300 m, and 100 m).</p>
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<p>Results from Moran’s I index for spatial autocorrelation.</p>
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<p>GAM predicted temperature map, UC model predicted map results, and actual calculated temperature map using Landsat images.</p>
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<p>Scatter plots with R2 and RMSE values for observed vs. predicted values for the Landsat images.</p>
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18 pages, 2150 KiB  
Article
Potential of Trichoderma asperellum as a Growth Promoter in Hydroponic Lettuce Cultivated in a Floating-Root System
by Aldo Gutiérrez-Chávez, Loreto Robles-Hernández, Brenda I. Guerrero, Ana Cecilia González-Franco, Gabriela Medina-Pérez, Angélica Anahí Acevedo-Barrera and Jared Hernández-Huerta
Plants 2025, 14(3), 382; https://doi.org/10.3390/plants14030382 - 26 Jan 2025
Viewed by 577
Abstract
The genus Trichoderma is widely used in agriculture as a biological agent and biofertilizer, enhancing crop yield and quality. However, its use in hydroponic systems is limited. This study evaluated the potential of Trichoderma asperellum as a growth promoter for lettuce (Lactuca [...] Read more.
The genus Trichoderma is widely used in agriculture as a biological agent and biofertilizer, enhancing crop yield and quality. However, its use in hydroponic systems is limited. This study evaluated the potential of Trichoderma asperellum as a growth promoter for lettuce (Lactuca sativa L.) cv. Starfighter RZ in a floating-root hydroponic system (FHS). T. asperellum strains (TaMFP1 and TaMFP2) were isolated from soil and identified morphologically and molecularly. The experiment used a completely randomized design with the following four treatments (n = 4): root spraying with TaMFP1, TaMFP2, T. harzianum (Trichospore®), and uninoculated plants (control). After 30 days, morphological, biochemical, and quality parameters were analyzed. All Trichoderma treatments significantly increased plant height (19.0%), root length (25.7%), total fresh biomass (76.4%), total dry biomass (82.63%), and number of leaves (18.18%). The nitrate levels in leaves were unaffected by TaMFP1 and TaMFP2, while Trichospore® reduced the nitrate content by 24.94%. The foliar nitrogen content increased with specific treatments, though the phosphorus and magnesium levels decreased. Visual quality traits, including appearance and firmness, remained unchanged. T. asperellum strains TaMFP1 and TaMFP2 enhanced vegetative growth without compromising quality, demonstrating their potential as sustainable tools for hydroponic lettuce production in controlled environments. Full article
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<p>Macro- and microscopic morphologies of <span class="html-italic">Trichoderma asperellum</span> strains in potato dextrose agar incubated at 28 °C for 96 h. <span class="html-italic">T. asperellum</span> TaMPF1: (<b>a</b>) colony; (<b>b</b>) presence of phialides and conidia. <span class="html-italic">T. asperellum</span> TaMPF2: (<b>c</b>) colony; (<b>d</b>) presence of phialides and conidia.</p>
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<p>Italian lettuce cv. Starfighter RZ treated with <span class="html-italic">Trichoderma</span> spp. cultivated in a floating-root hydroponic system in a greenhouse 30 days post-inoculation. Control = non-inoculated plants.</p>
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<p>Effect of <span class="html-italic">Trichoderma</span> spp. on the yield (<b>a</b>) and nitrates content (<b>b</b>) of Italian lettuce cv. Starfighter RZ in a floating-root hydroponic system under greenhouse conditions. Control = non-inoculated plants; Trichospore<sup>®</sup> = commercial product based on <span class="html-italic">Trichoderma harzianum</span>; TaMFP1 and TaMFP2 = <span class="html-italic">T. asperellum</span>. Bars with the same letter are not statistically different according to the Tukey test or the Games–Howell test *.</p>
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<p>Effect of <span class="html-italic">Trichoderma</span> spp. on the quality parameters of Italian lettuce cv. Starfighter RZ in a floating-root hydroponic system under greenhouse conditions. Control = non-inoculated plants; Trichospore<sup>®</sup> = commercial product based on <span class="html-italic">Trichoderma harzianum</span>; TaMFP1 and TaMFP2 = <span class="html-italic">T. asperellum</span>.</p>
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<p>Principal component analysis of the growth promotion in Italian lettuce plants cv. Starfighter RZ, treated with <span class="html-italic">Trichoderma</span> under a greenhouse floating-root hydroponic system (KMO 0.80, X<sup>2</sup> = 760, <span class="html-italic">p</span> &lt; 0.001). PC 1 and PC2 = principal components; NL = number of leaves; LA = leaf area; SL = stem length; RL = root length; RDW = root dry weight; SDW = stem dry weight; LDW = leaf dry weight; PH = plant height; Chl b = chlorophyll b; Chl a = chlorophyll a; Car = carotenoids.</p>
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25 pages, 4935 KiB  
Article
From Air to Space: A Comprehensive Approach to Optimizing Aboveground Biomass Estimation on UAV-Based Datasets
by Muhammad Nouman Khan, Yumin Tan, Lingfeng He, Wenquan Dong and Shengxian Dong
Forests 2025, 16(2), 214; https://doi.org/10.3390/f16020214 - 23 Jan 2025
Viewed by 684
Abstract
Estimating aboveground biomass (AGB) is vital for sustainable forest management and helps to understand the contributions of forests to carbon storage and emission goals. In this study, the effectiveness of plot-level AGB estimation using height and crown diameter derived from UAV-LiDAR, calibration of [...] Read more.
Estimating aboveground biomass (AGB) is vital for sustainable forest management and helps to understand the contributions of forests to carbon storage and emission goals. In this study, the effectiveness of plot-level AGB estimation using height and crown diameter derived from UAV-LiDAR, calibration of GEDI-L4A AGB and GEDI-L2A rh98 heights, and spectral variables derived from UAV-multispectral and RGB data were assessed. These calibrated AGB and height values and UAV-derived spectral variables were used to fit AGB estimations using a random forest (RF) regression model in Fuling District, China. Using Pearson correlation analysis, we identified 10 of the most important predictor variables in the AGB prediction model, including calibrated GEDI AGB and height, Visible Atmospherically Resistant Index green (VARIg), Red Blue Ratio Index (RBRI), Difference Vegetation Index (DVI), canopy cover (CC), Atmospherically Resistant Vegetation Index (ARVI), Red-Edge Normalized Difference Vegetation Index (NDVIre), Color Index of Vegetation (CIVI), elevation, and slope. The results showed that, in general, the second model based on calibrated AGB and height, Sentinel-2 indices, slope and elevation, and spectral variables from UAV-multispectral and RGB datasets with evaluation metric (for training: R2 = 0.941 Mg/ha, RMSE = 13.514 Mg/ha, MAE = 8.136 Mg/ha) performed better than the first model with AGB prediction. The result was between 23.45 Mg/ha and 301.81 Mg/ha, and the standard error was between 0.14 Mg/ha and 10.18 Mg/ha. This hybrid approach significantly improves AGB prediction accuracy and addresses uncertainties in AGB prediction modeling. The findings provide a robust framework for enhancing forest carbon stock assessment and contribute to global-scale AGB monitoring, advancing methodologies for sustainable forest management and ecological research. Full article
(This article belongs to the Section Forest Inventory, Modeling and Remote Sensing)
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<p>Study area description. (<b>a</b>) Location of Fuling District, Chongqing in China, (<b>b</b>) a Sentinel-2 composite image acquired from 1 August to 20 November 2024, covering the study area (Fuling District) (yellow outline), (<b>c</b>) a UAV image acquired on 2 July 2024, covering the Site 1 (red outline), and the field plots (orange outline), (<b>d</b>) UAV data acquired on 8 October 2024, covering Site 2 (purple outline), and (<b>e</b>) UAV data acquired on 8 October 2024, covering Site 3 (turquoise green outline).</p>
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<p>Conceptual framework of the study.</p>
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<p>UAV data acquisition and processing workflow. (<b>a</b>) Data acquisition using multi-rotor UAV-LiDAR sensor over real-time kinematic platform, (<b>b</b>) 3D visualization of UAV-LiDAR point cloud data, with point cloud segmentation and height curve of site 2 plot 30 (red outline), (<b>c</b>) a specification of UAV-multispectral and RGB platform showcase for each multispectral band over site 2 plot 30 (red outline), (<b>d</b>) UAV-RGB ortho-mosaic data over site 2 plot 30 (red outline).</p>
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<p>Scatter plot comparing GEDI-L2A rh98 heights and UAV-LiDAR mean heights.</p>
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<p>Scatter plot comparing GEDI-AGB and UAV-LiDAR AGB in Mg/ha.</p>
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<p>Calibrated GEDI footprints for AGB and height values in Fuling District.</p>
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<p>Pearson’s correlation analysis between UAV-multispectral spectral variables and the calibrated GEDI AGB.</p>
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<p>Pearson’s correlation analysis between UAV-RGB spectral variables and the calibrated GEDI AGB.</p>
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<p>Pearson’s correlation analysis between Sentinel-2 imagery-derived spectral variables and the calibrated GEDI AGB.</p>
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<p>First RF model prediction. (<b>A</b>) AGB prediction in Mg/ha. (<b>B</b>) Standard error of AGB prediction in Mg/ha.</p>
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<p>Model assessment statistics of the AGB prediction using calibrated GEDI-L4A AGB and GEDI-L2A heights for Fuling District. (<b>A</b>) Scatter plot of training data. (<b>B</b>) Scatter plot of testing data. (<b>C</b>) Scatter plot of validation data. (<b>D</b>) Variable importance (relative significance indicators within the model). Note: Relatively low significance indicators in (<b>D</b>): GNDVI, CIg, SR, MSAVI, and NDVI.</p>
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<p>Second RF model prediction. (<b>A</b>) AGB prediction in Mg/ha. (<b>B</b>) Standard error of AGB prediction in Mg/ha.</p>
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<p>Model assessment statistics of the AGB prediction using calibrated GEDI-L4A AGB, GEDI-L2A heights, and spectral variables from UAV-multispectral and RGB datasets for the Fuling District. (<b>A</b>) Scatter plot of training data. (<b>B</b>) Scatter plot of testing data. (<b>C</b>) Scatter plot of validation data. (<b>D</b>) Variable importance (relative significant parameters within the model). Note: Relatively low significance parameters in (<b>D</b>): Sentinel-2 (CIg, GNDVI, NDVI, MSAVI, SR), UAV-multispectral (Green, MSRre, NDVI), UAV-RGB (Green, Red, IPCA, IKAW).</p>
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16 pages, 4152 KiB  
Article
Analysis of the Changes in the Mechanical Properties of Branches of Salix Energy Plants After Shearing
by Natalia Walczak and Zbigniew Walczak
Forests 2025, 16(2), 206; https://doi.org/10.3390/f16020206 - 23 Jan 2025
Viewed by 355
Abstract
As a result of the energy crisis due, among other things, to climate change, most developed countries have taken steps with the main aim—among other things—of increasing the use of green energy sources that do not rely on fuels (including primarily liquid fuels) [...] Read more.
As a result of the energy crisis due, among other things, to climate change, most developed countries have taken steps with the main aim—among other things—of increasing the use of green energy sources that do not rely on fuels (including primarily liquid fuels) but use renewable energies. Plant biomass is a versatile substrate that can be used in many areas of the economy and production, but also for the production of various types of fuel. These range from rapeseed oil used as a component of biodiesel or maize starch for ethanol production to typically cellulosic plants such as energy willow, which can be used for direct combustion. The floodplain is home to this type of vegetation. It is characterized by great diversity in terms of geometric dimensions and mechanical and morphological properties. In addition, the location (easy access to water and sunlight) influences its potential energy value. Vegetation, thanks to favorable conditions, can achieve large weight gains in a relatively short period of time. Therefore, its properties should be carefully recognized in order to make more efficient use of energy and operating equipment used during harvesting. This paper presents an analysis of the changes in the elasticity of willow branches over a period of 16 days following harvesting. The changes were analyzed for branches taken from three different shrubs at three different plant height levels during the post-growth period. Based on the measurements carried out, the elastic modulus E of the shoots was estimated. The average modulus of elasticity ranged from about 4500 two days after cutting to about 5500 MPa 16 days after cutting and showed high variability, reaching even CV = 37%, both within a given shrub and depending on the measurement date. The results presented here indicate a high natural variability of mechanical parameters even within the same plant. Full article
(This article belongs to the Section Wood Science and Forest Products)
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<p>Deflection arrow measurement scheme.</p>
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<p>Photographs of willow bushes No. 2 and 3, from which the test material was taken.</p>
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<p>Summary of shrub diameter measurements.</p>
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<p>Summary of shrubs’ <span class="html-italic">w</span><sub>i</sub> measurements.</p>
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<p>Change in weight over time. Code n1 d/s/g n2; n1—bush number; d/s/g—sampling site designation (d—bottom, s—middle, g—top), n2—branch number from bush n1.</p>
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<p>Percentage weight loss of branches over time.</p>
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<p>Mean deflections over time for loads 6, 11, and 16 g.</p>
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<p>Average modules of elasticity for shrubs.</p>
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<p>Changes in the values of average elasticity moduli with time.</p>
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<p>Biplot for PCA and cluster analysis.</p>
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18 pages, 1919 KiB  
Article
Effect of Biodegradable Mulch and Different Synthetic Mulches on Growth and Yield of Field-Grown Small-Fruited Tomato (Lycopersicon esculentum Mill.)
by Katarzyna Adamczewska-Sowińska, Joanna Bykowy and Janina Jaworska
Agriculture 2025, 15(2), 212; https://doi.org/10.3390/agriculture15020212 - 19 Jan 2025
Viewed by 433
Abstract
Mulching is a widely adopted practice in vegetable cultivation globally. This technique employs various plastic materials, such as polyethylene (PE) film or polypropylene (PP) nonwoven fabric, with an increasing trend toward the use of biodegradable materials. Between 2014 and 2016, field experiments were [...] Read more.
Mulching is a widely adopted practice in vegetable cultivation globally. This technique employs various plastic materials, such as polyethylene (PE) film or polypropylene (PP) nonwoven fabric, with an increasing trend toward the use of biodegradable materials. Between 2014 and 2016, field experiments were conducted to evaluate the performance of the small-fruited tomato Intrigo F1 cultivated using synthetic mulches. The trials, designed as single-factor experiments employing a randomized block layout with three replicates, assessed plant morphological traits, yield, and the biological value of the tomato fruits. Weather conditions and the type of mulch applied had a pronounced influence on the quality of tomato plants and yield. Compared to the control, the use of black, red, and aluminum PE films and brown PP resulted in a 7.2% increase in plant height. All mulching treatments, except white film, increased the lateral spread of the plants by an average of 24.2%. Plants cultivated on red PE film exhibited a 26.4% increase in leaf count with respect to the control. Mulched treatments achieved an average increase of 19.6% in marketable yield. The highest marketable fruit yield was recorded with black nonwoven fabric mulch. Mulching had a significant effect on the chemical composition of tomato fruits. Fruits on biodegradable foil had the most potassium, lycopene, and polyphenols. Full article
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<p>Tomato plant height depending on the type of mulch in 2014–2016 (cm). * The same letters mark values belonging to the same homogeneous groups, determined on the basis of statistical analysis for α = 0.05. Type of mulch: PP nonwoven: black and brown; PE film: black, white, red; PE alu—aluminum film; Fbio—biodegradable film.</p>
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<p>Tomato plant stem diameter depending on the type of mulch in 2014–2016 (cm). * The same letters mark values belonging to the same homogeneous groups, determined on the basis of statistical analysis for α = 0.05. Type of mulch: PP nonwoven: black and brown; PE film: black, white, red; PE alu—aluminum film; Fbio—biodegradable.</p>
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<p>Tomato lateral spread depending on the type of mulch in 2014–2016 (cm). * The same letters mark values belonging to the same homogeneous groups, determined on the basis of statistical analysis for α = 0.05. Type of mulch: PP nonwoven: black and brown; PE film: black, white, red; PE alu—aluminum film; Fbio—biodegradable.</p>
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<p>Tomato leaf number depending on the type of mulch in 2014–2016. * The same letters mark values belonging to the same homogeneous groups, determined on the basis of statistical analysis for α = 0.05. Type of mulch: PP nonwoven: black and brown; PE film: black, white, red; PE alu—aluminum film; Fbio—biodegradable.</p>
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<p>Tomato plant height in July and August depending on the type of mulch. Average for 2014–2016 (cm). * The same letters mark values belonging to the same homogeneous groups, determined on the basis of statistical analysis for α = 0.05. Letters refer to type of mulch. Type of mulch: PP nonwoven: black and brown; PE film: black, white, red; PE alu—aluminum film; Fbio—biodegradable.</p>
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<p>Tomato plant diameter, span, and leaf number in August depending on the type of mulch. Average for 2014–2016. * The same letters mark values belonging to the same homogeneous groups, determined on the basis of statistical analysis for α = 0.05.</p>
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<p>Number of fruits per cluster depending on the type of mulch. Average for 2014–2016. * The same letters mark values belonging to the same homogeneous groups, determined on the basis of statistical analysis for α = 0.05. Type of mulch: PP nonwoven: black and brown; PE film: black, white, red; PE alu—aluminum film; Fbio—biodegradable film.</p>
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<p>Fruit mass from one cluster depending on the type of mulch in 2014–2016 (g). * The same letters mark values belonging to the same homogeneous groups, determined on the basis of statistical analysis for α = 0.05. Type of mulch: PP nonwoven: black and brown; PE film: black, white, red; PE alu—aluminum film; Fbio—biodegradable film.</p>
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19 pages, 4726 KiB  
Article
Establishment and Application of Biomass Model for Vegetation Condition Assessment After Ecological Restoration—Yixing Quarry Case Study
by Chaokui Huang, Yueping Wu, Shaohui Yang, Faming Zhang, Xiaokai Li, Huaqing Zhang and Xiaolong Zhang
Sustainability 2025, 17(2), 734; https://doi.org/10.3390/su17020734 - 17 Jan 2025
Viewed by 506
Abstract
Biomass is a vital index used to evaluate the vegetation rebuilding effect of mining slopes after restoration. It is essential to establish models for estimating the biomass and carbon storage of the vegetation community on mining slopes. Therefore, this paper establishes models for [...] Read more.
Biomass is a vital index used to evaluate the vegetation rebuilding effect of mining slopes after restoration. It is essential to establish models for estimating the biomass and carbon storage of the vegetation community on mining slopes. Therefore, this paper establishes models for the biomass and carbon storage of such vegetation, taking an abandoned quarry after ecological restoration in Yixing City, Jiangsu Province, as the research object. Firstly, the variables of the biomass estimation models were determined based on the correlation analysis results; the vegetation biomass model was comprehensively selected, and the accuracy of the optimal models was verified. Meanwhile, the carbon storage calculation model was established in combination with the carbon content and the growth pattern of vegetation. The results showed that (1) the optimal models were the cubic and linear functions, respectively, for the shrubs and herbs, while the relevant variables of the shrub and the herb plants were the average height multiplied by the diameter of each shrub plant (DH) and the average height multiplied by the coverage rate (CH), respectively, with the verification results of R2 > 0.814, RS > 2.8%, and RMA > 6%; and (2) in the restored mining slopes, the vegetation biomass was 120.264 t, including 10.586 t of herbs and 109.678 t of shrubs, and the vegetation carbon storage was 50.585 t, including 3.705 t of herbs and 46.880 t of shrubs. The proposed models have good prediction accuracy and reliability after quantitative evaluation and can be applied to the biomass estimation and carbon storage calculation of restored mining slopes, providing a reference for the environmental sustainability of post-mining areas and other ecologically restored slopes. Full article
(This article belongs to the Special Issue Sustainable Solutions for Land Reclamation and Post-mining Land Uses)
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<p>Location of the study area and images before ecological restoration.</p>
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<p>Land use map of the post-mining quarry.</p>
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<p>Slope area division in the study area.</p>
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<p>Diagram of each slope area: (<b>a</b>) slope A<sub>1</sub>; (<b>b</b>) slope A<sub>2</sub>; (<b>c</b>) slope A<sub>3</sub>; (<b>d</b>) slope A<sub>4</sub>; (<b>e</b>) slope A<sub>5</sub>; (<b>f</b>) slope A<sub>6</sub>; and (<b>g</b>) slope A<sub>7</sub>.</p>
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<p>Scheme diagram of vegetation community on the mining slopes (shrubs within the yellow dashed line and herbs on the slope): (<b>a</b>) slope A<sub>1</sub>; (<b>b</b>) slope A<sub>4</sub>.</p>
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<p>Biomass specific content of the aboveground biomass of each vegetation: (<b>a</b>) <span class="html-italic">Amorpha fruticosa</span> L.; (<b>b</b>) <span class="html-italic">Indigofera tinctoria</span> L.; (<b>c</b>) <span class="html-italic">Solidago canadensis</span> L.; and (<b>d</b>) <span class="html-italic">Erigeron canadensis</span> L.</p>
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<p>Correlation analysis heat map of biomass and variables: (<b>a</b>) <span class="html-italic">Amorpha fruticosa</span> L.; (<b>b</b>) <span class="html-italic">Indigofera tinctoria</span> L.; (<b>c</b>) <span class="html-italic">Solidago canadensis</span> L.; and (<b>d</b>) <span class="html-italic">Erigeron canadensis</span> L. (<span class="html-italic">D</span>: diameter of per-shrub plant, <span class="html-italic">A</span>: plant projected area, <span class="html-italic">V</span>: plant volume, <span class="html-italic">D<sup>2</sup>H</span>: square of diameter of per-shrub plant multiplied by average height, <span class="html-italic">DH</span>: average height multiplied by diameter of per-shrub plant, <span class="html-italic">H</span>: average height, <span class="html-italic">C</span>: coverage rate of the quadrat, <span class="html-italic">CH</span>: average height multiplied by coverage rate, <span class="html-italic">C<sup>2</sup>H</span>: square of coverage rate multiplied by average height, <span class="html-italic">CH<sup>2</sup></span>: coverage rate multiplied by square of average height, <span class="html-italic">W</span>: vegetation biomass, *: represents significant correlation, **: represents extremely significant correlation).</p>
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<p>Scheme diagram of the optimal biomass estimation model of (<b>a</b>) <span class="html-italic">Amorpha fruticosa</span> L.; (<b>b</b>) <span class="html-italic">Indigofera tinctoria</span> L.; (<b>c</b>) <span class="html-italic">Solidago canadensis</span> L.; and (<b>d</b>) <span class="html-italic">Erigeron canadensis</span> L. (<span class="html-italic">DH</span>: average height multiplied by diameter of per-shrub plant, <span class="html-italic">CH</span>: average height multiplied by coverage rate).</p>
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19 pages, 3051 KiB  
Article
Non-Thermal Plasma-Activated Water Enhances Nursery Production of Vegetables: A Species-Specific Study
by Silvia Locatelli, Stefano Triolone, Marina De Bonis, Giampaolo Zanin and Carlo Nicoletto
Agronomy 2025, 15(1), 209; https://doi.org/10.3390/agronomy15010209 - 16 Jan 2025
Viewed by 551
Abstract
Non-thermal plasma technology (NTP) has found widespread applications across several fields, including agriculture. Researchers have explored the use of NTP to improve plant growth and increase agricultural product quality using plasma-activated water (PAW). This technology has shown potential benefits in boosting seed germination, [...] Read more.
Non-thermal plasma technology (NTP) has found widespread applications across several fields, including agriculture. Researchers have explored the use of NTP to improve plant growth and increase agricultural product quality using plasma-activated water (PAW). This technology has shown potential benefits in boosting seed germination, promoting plant growth, as an effective defense against plant pathogens, and increasing systemic plant resistance. An experiment was set up over three different cultivation cycles to investigate the benefits of PAW administration on nursery production. Plasma-activated water was generated using two NTP intensities (PAW-HI = 600 mV; PAW-LI = 450 mV; CTR = tap water control) and manually applied to plants under greenhouse conditions. The species considered in the current study were tomato (Solanum lycopersicum L.), Swiss chard (Beta vulgaris L.), cabbage (Brassica oleracea L.), basil (Ocimum basilicum L.), and lettuce (Lactuca sativa L. var. Longifolia). The following morphological traits were measured at the end of each cycle and for each species: plant height (PH, cm), collar diameter (CD, mm), biomass (g), nutritional status (SPAD index), dry matter (DM, %), and chemical composition. The sturdiness index (SI) was determined by the PH-to-CD ratio. Results indicated a species-specific response to both PAW treatments compared to CTR. The plant height significantly increased in tomato (+11.9%) and cabbage (+5%) under PAW-HI treatment. In contrast, PAW-HI treatment negatively affected the PH in lettuce and basil (−18% and −9%, respectively). Swiss chard showed no significant response to either PAW-LI or PAW-HI treatments. Regarding DM, no significant differences were observed between the PAW treatments and CTR. However, an increase in total N content was detected in plant tissues across all species, except for basil, where no change was observed. The results suggest that PAW treatment has the potential to enhance vegetable nursery production, with species-specific responses observed in crops. Full article
(This article belongs to the Special Issue High-Voltage Plasma Applications in Agriculture)
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<p>Schematic representation of the water activation system using a non-thermal plasma generator.</p>
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<p>Effects of cultivation cycle (I cycle: May–June; II cycle: June–July; III cycle: September–October) and plasma-activated water treatment (CTR: control, PAW-LI: low-intensity, PAW-HI: high-intensity) on plant height (<b>A</b>), collar diameter (<b>B</b>), sturdiness index (<b>C</b>), and aerial biomass fresh weight (<b>D</b>) in tomato. Different letters indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) between treatments according to Tukey’s HSD test.</p>
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<p>Effects of cultivation cycle (I cycle: May–June; II cycle: June–July; III cycle: September–October) and plasma-activated water treatment (CTR: control; PAW-LI: low-intensity; PAW-HI: high-intensity) on plant height (<b>A</b>), collar diameter (<b>B</b>), sturdiness index (<b>C</b>), and aerial biomass fresh weight (<b>D</b>) in basil. Different letters indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) between treatments according to Tukey’s HSD test. ns = not significant differences.</p>
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<p>Effects of cultivation cycle (I cycle: May–June; II cycle: June–July; III cycle: September–October) and plasma-activated water treatment (CTR: control; PAW-LI: low-intensity; PAW-HI: high-intensity) on plant height (<b>A</b>), collar diameter (<b>B</b>), sturdiness index (<b>C</b>), and aerial biomass fresh weight (<b>D</b>) in Swiss chard. Different letters indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) between treatments according to Tukey’s HSD test.</p>
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<p>Effects of cultivation cycle (I cycle: May–June; II cycle: June–July; III cycle: September–October) and plasma-activated water treatment (CTR: control; PAW-LI: low-intensity; PAW-HI: high-intensity) on plant height (<b>A</b>), collar diameter (<b>B</b>), sturdiness index (<b>C</b>), and aerial biomass fresh weight (<b>D</b>) in cabbage. Different letters indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) between treatments according to Tukey’s HSD test.</p>
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<p>Effects of cultivation cycle (I cycle: May–June; II cycle: June–July; III cycle: September–October) and plasma-activated water treatment (CTR: control; PAW-LI: low-intensity; PAW-HI: high-intensity) on plant height (<b>A</b>), collar diameter (<b>B</b>), sturdiness index (<b>C</b>), and aerial biomass fresh weight (<b>D</b>) in lettuce. Different letters indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) between treatments according to Tukey’s HSD test.</p>
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17 pages, 4862 KiB  
Article
Modulation of Zn Ion Toxicity in Pisum sativum L. by Phycoremediation
by Zornitsa Karcheva, Zhaneta Georgieva, Svetoslav Anev, Detelina Petrova, Momchil Paunov, Miroslava Zhiponova and Ganka Chaneva
Plants 2025, 14(2), 215; https://doi.org/10.3390/plants14020215 - 14 Jan 2025
Viewed by 484
Abstract
Microalgae offer a promising alternative for heavy metal removal, and the search for highly efficient strains is ongoing. This study investigated the potential of two microalgae, Coelastrella sp. BGV (Chlorophyta) and Arthronema africanum Schwabe & Simonsen (Cyanoprokaryota), to bind zinc ions (Zn2 [...] Read more.
Microalgae offer a promising alternative for heavy metal removal, and the search for highly efficient strains is ongoing. This study investigated the potential of two microalgae, Coelastrella sp. BGV (Chlorophyta) and Arthronema africanum Schwabe & Simonsen (Cyanoprokaryota), to bind zinc ions (Zn2⁺) and protect higher plants. Hydroponically grown pea (Pisum sativum L.) seedlings were subjected to ZnSO4 treatment for 7 days in either a nutrient medium (Knop) or a microalgal suspension. The effects of increasing Zn2⁺ concentrations were evaluated through solution parameters, microalgal dry weight, pea growth (height, biomass), and physiological parameters, including leaf gas exchange, chlorophyll content, and normalized difference vegetation index (NDVI). Zinc accumulation in microalgal and plant biomass was also analyzed. The results revealed that microalgae increased pH and oxygen levels in the hydroponic medium while enhancing Zn accumulation in pea roots. At low ZnSO4 concentrations (2–5 mM), microalgal suspensions stimulated pea growth and photosynthetic performance. However, higher ZnSO4 levels (10–15 mM) caused Zn accumulation, leading to nutrient deficiencies and growth suppression in microalgae, which ultimately led to physiological disturbances in peas. Coelastrella sp. BGV exhibited greater tolerance to Zn stress and provided a stronger protective effect when co-cultivated with peas, highlighting its potential for phycoremediation applications. Full article
(This article belongs to the Section Plant Physiology and Metabolism)
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<p>Experimental co-cultivation systems under ZnSO<sub>4</sub> treatment on day 3. (<b>a</b>) <span class="html-italic">P. sativum</span> in control Knop medium. (<b>b</b>) <span class="html-italic">P. sativum</span> with <span class="html-italic">Coelastrella</span> sp. BGV. (<b>c</b>) <span class="html-italic">P. sativum</span> with <span class="html-italic">Arthronema africanum</span>.</p>
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<p>Effect of Zn treatment on microalgal growth. Samples were analyzed at 0, 3, 5, and 7 days (d). The data are represented as mean ± SE. Different letters indicate statistically significant differences in dry weight (DW) for each treatment at <span class="html-italic">p</span> &lt; 0.05. (<b>a</b>) <span class="html-italic">Coelastrella</span> sp. BGV. (<b>b</b>) <span class="html-italic">Arthronema africanum</span>.</p>
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<p>Effect of Zn treatment on <span class="html-italic">P. sativum</span> morphometric indicators. The three experimental systems with pea plants were subjected to a series of concentrations of ZnSO<sub>4</sub> for seven days. The data are represented as mean ± SE. Different letters indicate statistically significant differences between experimental systems for each treatment at <span class="html-italic">p</span> &lt; 0.05. (<b>a</b>,<b>c</b>,<b>e</b>) Plant height at days 3, 5, 7. (<b>b</b>,<b>d</b>,<b>f</b>) Biomass (fresh weight) at days 3, 5, 7.</p>
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<p>Effect of Zn treatment on physiological indicators in <span class="html-italic">P. sativum</span> leaves. The three experimental systems with pea plants were subjected to a series of concentrations of ZnSO<sub>4</sub> for five days. The data are represented as mean ± SE. Different letters indicate statistically significant differences between experimental systems for each treatment at <span class="html-italic">p</span> &lt; 0.05. (<b>a</b>) Net photosynthetic rate (P<sub>N</sub>). (<b>b</b>) Transpiration rate (E). (<b>c</b>) Chlorophyll (CHL) content. (<b>d</b>) Normalized difference vegetation index (NDVI).</p>
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<p>PCA of 10 experimental parameters representing differences between co-cultivation systems under increasing ZnSO<sub>4</sub>. Variants are given as circles, triangles, and squares, colored depending on the applied Zn<sup>2+</sup> concentration, while parameters are visualized as vectors.</p>
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20 pages, 927 KiB  
Article
Beyond BMI: Exploring Adolescent Lifestyle and Health Behaviours in Transylvania, Romania
by Alexandra-Ioana Roșioară, Bogdana Adriana Năsui, Nina Ciuciuc, Dana Manuela Sîrbu, Daniela Curșeu, Ștefan Cristian Vesa, Codruța Alina Popescu, Andreea Bleza and Monica Popa
Nutrients 2025, 17(2), 268; https://doi.org/10.3390/nu17020268 - 13 Jan 2025
Viewed by 813
Abstract
Background/Objectives: This study aimed to investigate the lifestyle and the behavioral factors that influence the nutritional status of adolescents from Transylvania, Romania. Methods: The Global School-Based Student Health Survey (GSHS) was used to collect data from 900 adolescents between 11 and 18 years [...] Read more.
Background/Objectives: This study aimed to investigate the lifestyle and the behavioral factors that influence the nutritional status of adolescents from Transylvania, Romania. Methods: The Global School-Based Student Health Survey (GSHS) was used to collect data from 900 adolescents between 11 and 18 years old from the Transylvania region, Romania. This study assessed nutritional status by calculating BMI indicators adjusted to Z-Score, cut-off points according to the World Health Organization (WHO), using self-reported weight and height; perceived health status; food vulnerability; physical activity; addictive behaviors (cigarette, alcohol and drug consumption); number of hours spent in front of the computer/phone; hand and oral hygiene; sitting time/day; and sleep. Multivariate logistic regression was used to establish the lifestyle factors that influenced nutritional status. Results: The results showed that 8.7% (n = 78) of girls and 15.2% (n = 137) boys were overweight and obese. In total, 75% of the respondents were engaged in sedentary behaviors, and 65.8% (n = 592) had more than 2 h/day of screen exposure, considering that 98.7% of the study population had a mobile phone. The Romanian adolescents had poor dietary behaviors: over 80% of them did not meet the recommended amount of vegetable and fruit intake per day. Increased BMI was associated with higher-strength physical exercise and with being a boy. Conclusions: While some positive trends are evident, such as good oral and hand hygiene and low prevalence of smoking and drug use, significant challenges remain in areas like nutrition, physical activity, alcohol consumption and screen time. Full article
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<p>Reporting flow diagram of sample selection in the study.</p>
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<p>Sex distribution of BMI in the sample size.</p>
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19 pages, 6391 KiB  
Article
Automated Tree Detection Using Image Processing and Multisource Data
by Grzegorz Dziczkowski, Barbara Probierz, Przemysław Juszczuk, Piotr Stefański, Tomasz Jach, Szymon Głowania and Jan Kozak
Appl. Sci. 2025, 15(2), 667; https://doi.org/10.3390/app15020667 - 11 Jan 2025
Viewed by 455
Abstract
This paper presents a method for the automatic detection and assessment of trees and tree-covered areas in Katowice, the capital of the Upper Silesian Industrial Region in southern Poland. The proposed approach utilizes satellite imagery and height maps, employing image-processing techniques and integrating [...] Read more.
This paper presents a method for the automatic detection and assessment of trees and tree-covered areas in Katowice, the capital of the Upper Silesian Industrial Region in southern Poland. The proposed approach utilizes satellite imagery and height maps, employing image-processing techniques and integrating data from various sources. We developed a data pipeline for gathering and pre-processing information, including vegetation data and numerical land-cover models, which were used to derive a new method for tree detection. Our findings confirm that automatic tree detection can significantly enhance the efficiency of urban tree management processes, contributing to the creation of greener and more resident-friendly cities. Full article
(This article belongs to the Special Issue Urban Geospatial Analytics Based on Big Data)
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<p>Example of a Color Infrared (CIR) image representing the normalized difference vegetation index (NDVI).</p>
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<p>Type of data used in this project.</p>
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<p>Image segmentation analysis: (A) road fragment;(B) field fragment; (C) segment with objects of different types.</p>
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<p>Image blurring for different kernel sizes: (<b>A</b>) <math display="inline"><semantics> <mrow> <mn>3</mn> <mo>×</mo> <mn>3</mn> </mrow> </semantics></math> kernel ; (<b>B</b>) <math display="inline"><semantics> <mrow> <mn>5</mn> <mo>×</mo> <mn>5</mn> </mrow> </semantics></math> kernel; (<b>C</b>) <math display="inline"><semantics> <mrow> <mn>7</mn> <mo>×</mo> <mn>7</mn> </mrow> </semantics></math> kernel; (<b>D</b>) without blurring.</p>
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<p>The general flowchart of the proposed approach: (<b>a</b>) initial data acquisition; (<b>b</b>) calculation of the numerical terrain data; (<b>c</b>) map segmentation and calculation of the dominant color; (<b>d</b>) color and height data binding followed by setting the default thresholds for the colors and height set by the decision maker.</p>
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<p>Proposed approach.</p>
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<p>Magnification of the first analyzed area.</p>
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<p>Magnification of the second analyzed area.</p>
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<p>Potential improvement using the modified edge-detection algorithm.</p>
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<p>Example of the area covered by various types of objects for all considered datasets.</p>
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21 pages, 3819 KiB  
Article
Improving Forest Canopy Height Mapping in Wuyishan National Park Through Calibration of ZiYuan-3 Stereo Imagery Using Limited Unmanned Aerial Vehicle LiDAR Data
by Kai Jian, Dengsheng Lu, Yagang Lu and Guiying Li
Forests 2025, 16(1), 125; https://doi.org/10.3390/f16010125 - 11 Jan 2025
Viewed by 491
Abstract
Forest canopy height (FCH) is a critical parameter for forest management and ecosystem modeling, but there is a lack of accurate FCH distribution in large areas. To address this issue, this study selected Wuyishan National Park in China as a case study to [...] Read more.
Forest canopy height (FCH) is a critical parameter for forest management and ecosystem modeling, but there is a lack of accurate FCH distribution in large areas. To address this issue, this study selected Wuyishan National Park in China as a case study to explore the calibration method for mapping FCH in a complex subtropical mountainous region based on ZiYuan-3 (ZY3) stereo imagery and limited Unmanned Aerial Vehicle (UAV) LiDAR data. Pearson’s correlation analysis, Categorical Boosting (CatBoost) feature importance analysis, and causal effect analysis were used to examine major factors causing extraction errors of digital surface model (DSM) data from ZY3 stereo imagery. Different machine learning algorithms were compared and used to calibrate the DSM and FCH results. The results indicate that the DSM extraction accuracy based on ZY3 stereo imagery is primarily influenced by slope aspect, elevation, and vegetation characteristics. These influences were particularly notable in areas with a complex topography and dense vegetation coverage. A Bayesian-optimized CatBoost model with directly calibrating the original FCH (the difference between the DSM from ZY3 and high-precision digital elevation model (DEM) data) demonstrated the best prediction performance. This model produced the FCH map at a 4 m spatial resolution, the root mean square error (RMSE) was reduced from 6.47 m based on initial stereo imagery to 3.99 m after calibration, and the relative RMSE (rRMSE) was reduced from 36.52% to 22.53%. The study demonstrates the feasibility of using ZY3 imagery for regional forest canopy height mapping and confirms the superior performance of using the CatBoost algorithm in enhancing FCH calibration accuracy. These findings provide valuable insights into the multidimensional impacts of key environmental factors on FCH extraction, supporting precise forest monitoring and carbon stock assessment in complex terrains in subtropical regions. Full article
(This article belongs to the Special Issue Mapping and Modeling Forests Using Geospatial Technologies)
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<p>The physical features of Wuyishan National Park ((<b>a</b>)—the location with the extent of ZiYuan-3 data; (<b>b</b>)—the forest distribution in 2022 and the typical sites of UAV-LiDAR).</p>
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<p>Framework for mapping forest canopy height using ZiYuan-3 and UAV LiDAR data. DSM<sub>r</sub> and FCH<sub>r</sub>—digital surface model and forest canopy height from UAV LiDAR data, respectively, as reference data; DSM—the original DSM from ZiYuan-3 stereo data, FCH—original FCH (DSM-DEM).</p>
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<p>Relationships between investigated factors and DSM<sub>error</sub> ((<b>a</b>)—Pearson’s correlation coefficients; (<b>b</b>)—Variable importance rankings from CatBoost; (<b>c</b>)—The causal effect rankings from the causal inference analysis; DSM<sub>error</sub>—DSM residuals; LS—Slope length and steepness factor; VCI—Vegetation coverage index, EVI—Enhanced vegetation index; kNDVI—The kernel Normalized Difference Vegetation Index).</p>
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<p>Causal pathway diagram of various factors affecting DSM<sub>error</sub> (DSM residuals) (DSM—Digital surface model; LS—Slope length and steepness factor; VCI—Vegetation coverage index; EVI—Enhanced vegetation index; kNDVI—The kernel Normalized Difference Vegetation Index). In this diagram, the edge values quantify the causal effects: their absolute values indicate effect strength, while their signs (positive or negative) denote the direction of the relationship.</p>
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<p>Scatter plots for comparison of the original FCH and the calibrated FCH against FCH<sub>r</sub> across different forest types (FCH represents forest canopy height obtained by subtracting the DEM from the ZY3-derived DSM, the calibrated FCH (FCH<sub>dc</sub>) represents the canopy height obtained by the direct calibration method using CatBoost, FCH<sub>r</sub> represents the reference FCH derived from UAV LiDAR).</p>
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<p>Spatial distribution of forest canopy height (FCH) within Wuyishan National Park. ((<b>a</b>): FCH generated from the difference between the digital surface model from ZY3 stereo imagery and the digital elevation model from airborne LiDAR, (<b>b</b>): the calibrated FCH using the CatBoost in the direct calibration method).</p>
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<p>Accuracy comparison between the calibrated FCH and the ETH Global Sentinel−2 10 m Canopy Height Product (2020) [<a href="#B53-forests-16-00125" class="html-bibr">53</a>] ((<b>a</b>) FCH generated by this research, (<b>b</b>) ETH_FCH product produced by [<a href="#B53-forests-16-00125" class="html-bibr">53</a>]).</p>
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21 pages, 14704 KiB  
Article
Effectiveness Trade-Off Between Green Spaces and Built-Up Land: Evaluating Trade-Off Efficiency and Its Drivers in an Expanding City
by Xinyu Dong, Yanmei Ye, Tao Zhou, Dagmar Haase and Angela Lausch
Remote Sens. 2025, 17(2), 212; https://doi.org/10.3390/rs17020212 - 9 Jan 2025
Viewed by 560
Abstract
Urban expansion encroaches on green spaces and weakens ecosystem services, potentially leading to a trade-off between ecological conditions and socio-economic growth. Effectively coordinating the two elements is essential for achieving sustainable development goals at the urban scale. However, few studies have measured urban–ecological [...] Read more.
Urban expansion encroaches on green spaces and weakens ecosystem services, potentially leading to a trade-off between ecological conditions and socio-economic growth. Effectively coordinating the two elements is essential for achieving sustainable development goals at the urban scale. However, few studies have measured urban–ecological linkage in terms of trade-off. In this study, we propose a framework by linking the degraded ecological conditions and urban land use efficiency from a return on investment perspective. Taking a rapidly expanding city as a case study, we comprehensively quantified urban–ecological conditions in four aspects: urban heat island, flood regulating service, habitat quality, and carbon sequestration. These conditions were assessed on 1 km2 grids, along with urban land use efficiency at the same spatial scale. We employed the slack-based measure model to evaluate trade-off efficiency and applied the geo-detector method to identify its driving factors. Our findings reveal that while urban–ecological conditions in Zhengzhou’s periphery degraded over the past two decades, the inner city showed improvement in urban heat island and carbon sequestration. Trade-off efficiency exhibited an overall upward trend during 2000–2020, despite initial declines in some inner city areas. Interaction detection demonstrates significant synergistic effects between pairs of drivers, such as the Normalized Difference Vegetation Index and building height, and the number of patches of green spaces and the patch cohesion index of built-up land, with q-values of 0.298 and 0.137, respectively. In light of the spatiotemporal trend of trade-off efficiency and its drivers, we propose adaptive management strategies. The framework could serve as guidance to assist decision-makers and urban planners in monitoring urban–ecological conditions in the context of urban expansion. Full article
(This article belongs to the Section Ecological Remote Sensing)
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<p>Location and boundary of the study area.</p>
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<p>Methodological framework of the study.</p>
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<p>Urban–ecological conditions; (<b>a</b>–<b>c</b>) are urban heat islands in 2000, 2010, and 2020, respectively; (<b>d</b>–<b>f</b>) are flood regulating services (runoff depth) in 2000, 2010, and 2020, respectively; (<b>g</b>–<b>i</b>) are the habitat quality in 2000, 2010, and 2020, respectively; and (<b>j</b>–<b>l</b>) are the carbon sequestration in 2000, 2010, and 2020, respectively.</p>
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<p>Urban land use efficiency: (<b>a</b>) 2000, (<b>b</b>) 2010, (<b>c</b>) 2020.</p>
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<p>Spatial pattern of trade-off efficiency: (<b>a</b>) 2000, (<b>b</b>) 2010, (<b>c</b>) 2020.</p>
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<p>Distribution of trade-off efficiency.</p>
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<p>Spatiotemporal change in trade-off efficiency: (<b>a</b>) 2000–2010, (<b>b</b>) 2010–2020.</p>
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<p>Result of interaction detection. Note: single-variable attenuation is Min(q(X1), q(X2)) &lt; q(X1∩X2) &lt; Max(q(X1), q(X2)); dual-variable enhancement is q(X1∩X2) &gt; Max(q(X1), q(X2)); and non-linear enhancement is q(X1∩X2) &gt; q(X1) + q(X2).</p>
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