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14 pages, 5194 KiB  
Communication
A Holistic Irrigation Advisory Policy Scheme by the Hellenic Agricultural Organization: An Example of a Successful Implementation in Crete, Greece
by Nektarios N. Kourgialas
Water 2024, 16(19), 2769; https://doi.org/10.3390/w16192769 (registering DOI) - 28 Sep 2024
Abstract
The aim of this communication article is to present a successful irrigation advisory scheme on the island of Crete (Greece) provided by the Hellenic Agricultural Organization (ELGO DIMITRA), which is well adapted to the different needs of farmers and water management agencies. The [...] Read more.
The aim of this communication article is to present a successful irrigation advisory scheme on the island of Crete (Greece) provided by the Hellenic Agricultural Organization (ELGO DIMITRA), which is well adapted to the different needs of farmers and water management agencies. The motivation to create this advisory scheme stems from the need to save water resources while ensuring optimal production in a region like Crete where droughts seem to occur more and more frequently in recent years. This scheme/approach has three different levels of implementation (components) depending on the spatial level and end-users’ needs. The first level concerns the weekly irrigation bulletins in the main agricultural areas of the island with the aim of informing farmers and local water managers about crop irrigation needs. The second level concerns an innovative digital web-based platform for the precise determination of the irrigation needs of Crete’s crops at a parcel level as well as optimal adaptation strategies in the context of climate change. In this platform, important features such as real-time meteorological information, spatial data on the cultivation type of parcels, validated algorithms for calculating crop irrigation needs, an accurate soil texture map derived from satellite images, and appropriate agronomic practices to conserve water based on cultivation and the geomorphology of a farm are considered. The third level of the proposed management approach includes an open-source Internet of Things (IoT) intelligent irrigation system for optimal individual parcel irrigation scheduling. This IoT system includes soil moisture and atmospheric sensors installed on the field, as well as the corresponding laboratory soil hydraulic characterization service. This third-level advisory approach provides farmers with specialized information on the automated irrigation system and optimization of irrigation water use. All the above irrigation advisory approaches have been implemented and evaluated by end-users with a very high degree of satisfaction in terms of effectiveness and usability. Full article
23 pages, 5167 KiB  
Article
Optical Characterization of Coastal Waters with Atmospheric Correction Errors: Insights from SGLI and AERONET-OC
by Hiroto Higa, Masataka Muto, Salem Ibrahim Salem, Hiroshi Kobayashi, Joji Ishizaka, Kazunori Ogata, Mitsuhiro Toratani, Kuniaki Takahashi, Fabrice Maupin and Stephane Victori
Remote Sens. 2024, 16(19), 3626; https://doi.org/10.3390/rs16193626 (registering DOI) - 28 Sep 2024
Abstract
This study identifies the characteristics of water regions with negative normalized water-leaving radiance (nLw(λ)) values in the satellite observations of the Second-generation Global Imager (SGLI) sensor aboard the Global Change Observation Mission–Climate (GCOM-C) satellite. SGLI Level-2 [...] Read more.
This study identifies the characteristics of water regions with negative normalized water-leaving radiance (nLw(λ)) values in the satellite observations of the Second-generation Global Imager (SGLI) sensor aboard the Global Change Observation Mission–Climate (GCOM-C) satellite. SGLI Level-2 data, along with atmospheric and in-water optical properties measured by the sun photometers in the AErosol RObotic NETwork-Ocean Color (AERONET-OC) from 26 sites globally, are utilized in this study. The focus is particularly on Tokyo Bay and the Ariake Sea, semi-enclosed water regions in Japan where previous research has pointed out the occurrence of negative nLw(λ) values due to atmospheric correction with SGLI. The study examines the temporal changes in atmospheric and in-water optical properties in these two regions, and identifies the characteristics of regions prone to negative nLw(λ) values due to atmospheric correction by comparing the optical properties of these regions with those of 24 other AERONET-OC sites. The time series results of nLw(λ) and the single-scattering albedo (ω(λ)) obtained by the sun photometers at the two sites in Tokyo Bay and Ariake Sea, along with SGLI nLw(λ), indicate the occurrence of negative values in SGLI nLw(λ) in blue band regions, which are mainly attributed to the inflow of absorptive aerosols. However, these negative values are not entirely explained by ω(λ) at 443 nm alone. Additionally, a comparison of in situ nLw(λ) measurements in Tokyo Bay and the Ariake Sea with nLw(λ) values obtained from 24 other AERONET-OC sites, as well as the inherent optical properties (IOPs) estimated through the Quasi-Analytical Algorithm version 5 (QAA_v5), identified five sites—Gulf of Riga, Long Island Sound, Lake Vanern, the Tokyo Bay, and Ariake Sea—as regions where negative nLw(λ) values are more likely to occur. These regions also tend to have lower nLw(λ)  values at shorter wavelengths. Furthermore, relatively high light absorption by phytoplankton and colored dissolved organic matter, plus non-algal particles, was confirmed in these regions. This occurs because atmospheric correction processing excessively subtracts aerosol light scattering due to the influence of aerosol absorption, increasing the probability of the occurrence of negative nLw(λ) values. Based on the analysis of atmospheric and in-water optical measurements derived from AERONET-OC in this study, it was found that negative nLw(λ)  values due to atmospheric correction are more likely to occur in water regions characterized by both the presence of absorptive aerosols in the atmosphere and high light absorption by in-water substances. Full article
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Figure 1

Figure 1
<p>Map of target water regions and installation locations of SeaPRISM in AERONET-OC: (<b>a</b>) Kemigawa Offshore Tower in Tokyo Bay, Japan, (<b>b</b>) Ariake Sea Observation Tower in Japan, and (<b>c</b>) AERONET-OC sites in various countries. The numbers shown next to each site correspond to the AERONET-OC site numbers listed in <a href="#remotesensing-16-03626-t001" class="html-table">Table 1</a>.</p>
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<p>SeaPRISM optical measurements for Tokyo Bay (top panels) and the Ariake Sea (bottom panels). Panels (<b>a</b>,<b>d</b>) show <math display="inline"><semantics> <mrow> <mi>n</mi> <msub> <mrow> <mi>L</mi> </mrow> <mrow> <mi>w</mi> </mrow> </msub> <mo>(</mo> <mi>λ</mi> <mo>)</mo> </mrow> </semantics></math>, (<b>b</b>,<b>e</b>) show <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>τ</mi> </mrow> <mrow> <mi>a</mi> </mrow> </msub> <mfenced separators="|"> <mrow> <mi>λ</mi> </mrow> </mfenced> </mrow> </semantics></math>, and (<b>c</b>,<b>f</b>) show <math display="inline"><semantics> <mrow> <mi>ω</mi> <mo>(</mo> <mi>λ</mi> <mo>)</mo> </mrow> </semantics></math> for Tokyo Bay and the Ariake Sea, respectively. Gray lines represent individual measurement samples, with square markers indicating the measured wavelengths. Black lines denote the mean values across all measured samples, with circle markers representing the observed wavelengths and error bars indicating the standard deviation.</p>
Full article ">Figure 3
<p>SeaPRISM <math display="inline"><semantics> <mrow> <mi>n</mi> <msub> <mrow> <mi>L</mi> </mrow> <mrow> <mi>w</mi> </mrow> </msub> <mo>(</mo> <mi>λ</mi> <mo>)</mo> </mrow> </semantics></math> measurements for Tokyo Bay, illustrating the relationships between <math display="inline"><semantics> <mrow> <mi>n</mi> <msub> <mrow> <mi>L</mi> </mrow> <mrow> <mi>w</mi> </mrow> </msub> <mo>(</mo> <mi>λ</mi> <mo>)</mo> </mrow> </semantics></math> and water quality parameters. (<b>a</b>) Shows individual measurements of <span class="html-italic">nL<sub>w</sub>(λ)</span> across varying Chl-a concentrations, and (<b>b</b>) is the mean of each Chl-a range. Relationship between <math display="inline"><semantics> <mrow> <mi>n</mi> <msub> <mrow> <mi>L</mi> </mrow> <mrow> <mi>w</mi> </mrow> </msub> <mo>(</mo> <mi>λ</mi> <mo>)</mo> </mrow> </semantics></math> and salinity for (<b>c</b>) individual salinity values and (<b>d</b>) mean of each salinity range. Circles in each spectrum represent the observed wavelengths.</p>
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<p>Time series results of <math display="inline"><semantics> <mrow> <mi>n</mi> <msub> <mrow> <mi>L</mi> </mrow> <mrow> <mi>w</mi> </mrow> </msub> <mo>(</mo> <mn>412</mn> <mo>)</mo> </mrow> </semantics></math> and <math display="inline"><semantics> <mrow> <mi>ω</mi> <mfenced separators="|"> <mrow> <mn>443</mn> </mrow> </mfenced> </mrow> </semantics></math> measured by SeaPRISM for Tokyo Bay and the Ariake Sea from January 2020 to December 2021. For <math display="inline"><semantics> <mrow> <mi>n</mi> <msub> <mrow> <mi>L</mi> </mrow> <mrow> <mi>w</mi> </mrow> </msub> <mo>(</mo> <mn>412</mn> <mo>)</mo> </mrow> </semantics></math> results, the blue and oranges lines represent the values measured by SeaPRISM, and the values obtained after atmospheric correction by SGLI, respectively. For <math display="inline"><semantics> <mrow> <mi>ω</mi> <mfenced separators="|"> <mrow> <mn>443</mn> </mrow> </mfenced> </mrow> </semantics></math> results, “x” symbols indicate measurement samples, and the black lines represent the monthly averages (error bars show their standard deviations).</p>
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<p>Comparison of <math display="inline"><semantics> <mrow> <mi>n</mi> <msub> <mrow> <mi>L</mi> </mrow> <mrow> <mi>w</mi> </mrow> </msub> <mo>(</mo> <mi>λ</mi> <mo>)</mo> </mrow> </semantics></math> measured by SeaPRISM and estimated by SGLI. The target wavelengths are 412, 443, 490, 530, 565, and 673.5 nm. Results for 23 AERONET-OC sites are shown. The left panel for each wavelength shows the individual measurements during the observation period, and the right panel for each wavelength shows the average measured and estimated values for each site, along with their standard deviations.</p>
Full article ">Figure 6
<p>Scatter plots of SGLI-derived <math display="inline"><semantics> <mrow> <mi>n</mi> <msub> <mrow> <mi>L</mi> </mrow> <mrow> <mi>w</mi> </mrow> </msub> <mo>(</mo> <mn>412</mn> <mo>)</mo> </mrow> </semantics></math> versus SeaPRISM-measured <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>τ</mi> </mrow> <mrow> <mi>a</mi> </mrow> </msub> <mo>(</mo> <mn>412</mn> <mo>)</mo> </mrow> </semantics></math> (top panels) and scatter plots of SGLI-derived <math display="inline"><semantics> <mrow> <mi>n</mi> <msub> <mrow> <mi>L</mi> </mrow> <mrow> <mi>w</mi> </mrow> </msub> <mo>(</mo> <mn>412</mn> <mo>)</mo> </mrow> </semantics></math> versus <math display="inline"><semantics> <mrow> <mi>ω</mi> <mo>(</mo> <mn>443</mn> <mo>)</mo> </mrow> </semantics></math> (bottom panels) for various AERONET-OC sites. Red and blue circles indicate Tokyo Bay and the Ariake Sea, respectively. The left plots show individual sample points for each target product and the right plots show the average results and standard deviation for each water region (see legend).</p>
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<p>Relationship between <math display="inline"><semantics> <mrow> <mi>n</mi> <msub> <mrow> <mi>L</mi> </mrow> <mrow> <mi>w</mi> </mrow> </msub> <mo>(</mo> <mi>λ</mi> <mo>)</mo> </mrow> </semantics></math> from SeaPRISM and SGLI in water regions with more than 30 matchup data points. The left panel compares individual samples, and the right panel shows the mean and standard deviation for each water region. The colored regions represent the five water regions where negative <math display="inline"><semantics> <mrow> <mi>n</mi> <msub> <mrow> <mi>L</mi> </mrow> <mrow> <mi>w</mi> </mrow> </msub> <mo>(</mo> <mi>λ</mi> <mo>)</mo> </mrow> </semantics></math> values are more likely to occur (red—Tokyo Bay, blue—the Ariake Sea, pink—the Gulf of Riga, green—Long Island Sound, yellow—Lake Beynell). The gray circles denote results from the other 10 water regions.</p>
Full article ">Figure 8
<p>Relationships between <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>a</mi> </mrow> <mrow> <mi>p</mi> <mi>h</mi> </mrow> </msub> <mo>(</mo> <mn>443</mn> <mo>)</mo> </mrow> </semantics></math> and <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>b</mi> </mrow> <mrow> <mi>b</mi> <mi>p</mi> </mrow> </msub> <mo>(</mo> <mn>565</mn> <mo>)</mo> </mrow> </semantics></math> and between <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>a</mi> </mrow> <mrow> <mi>d</mi> <mi>g</mi> </mrow> </msub> <mo>(</mo> <mn>443</mn> <mo>)</mo> </mrow> </semantics></math> and <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>b</mi> </mrow> <mrow> <mi>b</mi> <mi>p</mi> </mrow> </msub> <mo>(</mo> <mn>565</mn> <mo>)</mo> </mrow> </semantics></math> estimated using QAA based on <math display="inline"><semantics> <mrow> <mi>n</mi> <msub> <mrow> <mi>L</mi> </mrow> <mrow> <mi>w</mi> </mrow> </msub> <mo>(</mo> <mi>λ</mi> <mo>)</mo> </mrow> </semantics></math> measurements from SeaPRISM as input. The top row (<b>a</b>,<b>c</b>) shows the relationships for individual samples and the bottom row (<b>b</b>,<b>d</b>) shows the mean and standard deviation for each water region. The colored regions represent the five water regions where negative <math display="inline"><semantics> <mrow> <mi>n</mi> <msub> <mrow> <mi>L</mi> </mrow> <mrow> <mi>w</mi> </mrow> </msub> <mo>(</mo> <mi>λ</mi> <mo>)</mo> </mrow> </semantics></math> values are more likely to occur (red—Tokyo Bay, blue—the Ariake Sea, pink—the Gulf of Riga, green—Long Island Sound, yellow—Lake Beynell). The gray circles denote results from the other 10 water regions.</p>
Full article ">Figure 9
<p>Relationship between <math display="inline"><semantics> <mrow> <mi>ω</mi> <mo>(</mo> <mn>443</mn> <mo>)</mo> </mrow> </semantics></math> estimated by inversion using SeaPRISM and <math display="inline"><semantics> <mrow> <mi>n</mi> <msub> <mrow> <mi>L</mi> </mrow> <mrow> <mi>w</mi> </mrow> </msub> <mo>(</mo> <mn>443</mn> <mo>)</mo> </mrow> </semantics></math>. The left panel shows the relationship for individual samples and the right panel shows the mean and standard deviation for each water region. The colored regions represent the five water regions where negative <math display="inline"><semantics> <mrow> <mi>n</mi> <msub> <mrow> <mi>L</mi> </mrow> <mrow> <mi>w</mi> </mrow> </msub> <mo>(</mo> <mi>λ</mi> <mo>)</mo> </mrow> </semantics></math> values are more likely to occur (red—Tokyo Bay, blue—the Ariake Sea, pink—the Gulf of Riga, green—Long Island Sound, yellow—Lake Beynell). The gray circles denote results from the other 10 water regions.</p>
Full article ">
35 pages, 3463 KiB  
Review
A Review of Recent Techniques for Human Activity Recognition: Multimodality, Reinforcement Learning, and Language Models
by Ugonna Oleh, Roman Obermaisser and Abu Shad Ahammed
Algorithms 2024, 17(10), 434; https://doi.org/10.3390/a17100434 (registering DOI) - 28 Sep 2024
Abstract
Human Activity Recognition (HAR) is a rapidly evolving field with the potential to revolutionise how we monitor and understand human behaviour. This survey paper provides a comprehensive overview of the state-of-the-art in HAR, specifically focusing on recent techniques such as multimodal techniques, Deep [...] Read more.
Human Activity Recognition (HAR) is a rapidly evolving field with the potential to revolutionise how we monitor and understand human behaviour. This survey paper provides a comprehensive overview of the state-of-the-art in HAR, specifically focusing on recent techniques such as multimodal techniques, Deep Reinforcement Learning and large language models. It explores the diverse range of human activities and the sensor technologies employed for data collection. It then reviews novel algorithms used for Human Activity Recognition with emphasis on multimodality, Deep Reinforcement Learning and large language models. It gives an overview of multimodal datasets with physiological data. It also delves into the applications of HAR in healthcare. Additionally, the survey discusses the challenges and future directions in this exciting field, highlighting the need for continued research and development to fully realise the potential of HAR in various real-world applications. Full article
(This article belongs to the Section Algorithms for Multidisciplinary Applications)
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Figure 1

Figure 1
<p>UML diagram of human activities.</p>
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<p>UML diagrams of sensors used in HAR.</p>
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31 pages, 1375 KiB  
Article
A Circular Touch Mode Capacitive Rainfall Sensor: Analytical Solution and Numerical Design and Calibration
by Xiao-Ting He, Jun-Song Ran, Ji Wu, Fei-Yan Li and Jun-Yi Sun
Sensors 2024, 24(19), 6291; https://doi.org/10.3390/s24196291 (registering DOI) - 28 Sep 2024
Abstract
A circular capacitive rainfall sensor can operate from non-touch mode to touch mode; that is, under the action of enough rainwater, its movable electrode plate can form a circular contact area with its fixed electrode plate. Therefore, the weight of rainwater is borne [...] Read more.
A circular capacitive rainfall sensor can operate from non-touch mode to touch mode; that is, under the action of enough rainwater, its movable electrode plate can form a circular contact area with its fixed electrode plate. Therefore, the weight of rainwater is borne by only its movable electrode plate in non-touch mode operation but by both its movable and fixed electrode plates in touch mode operation, and the total capacitance of its touch mode operation is much larger than that of its non-touch mode operation. Essential to its numerical design and calibration is the ability to predict the deflection shape of its moveable electrode plate to determine its total capacitance. This requires the analytical solution to the fluid–structure interaction problem of its movable electrode plate under rainwater. In our previous work, only the analytical solution for the fluid–structure interaction problem before its movable electrode plate touches its fixed electrode plate was obtained, and how to numerically design and calibrate a circular non-touch mode capacitive rainfall sensor was illustrated. In this paper, the analytical solution for the fluid–structure interaction problem after its movable electrode plate touches its fixed electrode plate is obtained, and how to numerically design and calibrate a circular touch mode capacitive rainfall sensor is illustrated for the first time. The numerical results show that the total capacitance and rainwater volume when the circular capacitive rainfall sensor operates in touch mode is indeed much larger than that when the same circular capacitive rainfall sensor operates in non-touch mode, and that the average increase in the maximum membrane stress per unit rainwater volume when the circular capacitive rainfall sensor operates in touch mode can be about 20 times smaller than that when the same circular capacitive rainfall sensor operates in non-touch mode. This is where the circular touch mode capacitive rainfall sensor excels. Full article
(This article belongs to the Special Issue Recent Advances in Low Cost Capacitive Sensors)
16 pages, 4983 KiB  
Article
Pixel-Level Decision Fusion for Land Cover Classification Using PolSAR Data and Local Pattern Differences
by Spiros Papadopoulos, Vassilis Anastassopoulos and Georgia Koukiou
Electronics 2024, 13(19), 3846; https://doi.org/10.3390/electronics13193846 (registering DOI) - 28 Sep 2024
Abstract
Combining various viewpoints to produce coherent and cohesive results requires decision fusion. These methodologies are essential for synthesizing data from multiple sensors in remote sensing classification in order to make conclusive decisions. Using fully polarimetric Synthetic Aperture Radar (PolSAR) imagery, our study combines [...] Read more.
Combining various viewpoints to produce coherent and cohesive results requires decision fusion. These methodologies are essential for synthesizing data from multiple sensors in remote sensing classification in order to make conclusive decisions. Using fully polarimetric Synthetic Aperture Radar (PolSAR) imagery, our study combines the benefits of both approaches for detection by extracting Pauli’s and Krogager’s decomposition components. The Local Pattern Differences (LPD) method was employed on every decomposition component for pixel-level texture feature extraction. These extracted features were utilized to train three independent classifiers. Ultimately, these findings were handled as independent decisions for each land cover type and were fused together using a decision fusion rule to produce complete and enhanced classification results. As part of our approach, after a thorough examination, the most appropriate classifiers and decision rules were exploited, as well as the mathematical foundations required for effective decision fusion. Incorporating qualitative and quantitative information into the decision fusion process ensures robust and reliable classification results. The innovation of our approach lies in the dual use of decomposition methods and the application of a simple but effective decision fusion strategy. Full article
(This article belongs to the Special Issue Artificial Intelligence in Image Processing and Computer Vision)
13 pages, 2491 KiB  
Article
Development of a Compact NDIR CO2 Gas Sensor for a Portable Gas Analyzer
by Maosen Xu, Wei Tian, Yuzhe Lin, Yan Xu and Jifang Tao
Micromachines 2024, 15(10), 1203; https://doi.org/10.3390/mi15101203 (registering DOI) - 28 Sep 2024
Abstract
A carbon dioxide (CO2) gas sensor based on non-dispersive infrared (NDIR) technology has been developed and is suitable for use in portable devices for high-precision CO2 detection. The NDIR gas sensor comprises a MEMS infrared emitter, a MEMS thermopile detector [...] Read more.
A carbon dioxide (CO2) gas sensor based on non-dispersive infrared (NDIR) technology has been developed and is suitable for use in portable devices for high-precision CO2 detection. The NDIR gas sensor comprises a MEMS infrared emitter, a MEMS thermopile detector with an integrated optical filter, and a compact gas cell with high optical coupling efficiency. A dual-ellipsoid mirror optical system was designed, and based on optical simulation analysis, the structure of the dual-ellipsoid reflective gas chamber was designed and optimized, achieving a coupling efficiency of up to 54%. Optical and thermal simulations were conducted to design the sensor structure, considering thermal management and light analysis. By optimizing the gas cell structure and conditioning circuit, we effectively reduced the sensor’s baseline noise, enhancing the overall reliability and stability of the system. The sensor’s dimensions were 20 mm × 10 mm × 4 mm (L × W × H), only 15% of the size of traditional NDIR gas sensors with equivalent detection resolution. The developed sensor offers high sensitivity and low noise, with a sensitivity of 15 μV/ppm, a detection limit of 90 ppm, and a resolution of 30 ppm. The total power consumption of the whole sensor system is 6.5 mW, with a maximum power consumption of only 90 mW. Full article
15 pages, 5338 KiB  
Article
Research on the Fabrication and Parameters of a Flexible Fiber Optic Pressure Sensor with High Sensitivity
by Huixin Zhang, Jing Wu and Chencheng Gao
Photonics 2024, 11(10), 919; https://doi.org/10.3390/photonics11100919 (registering DOI) - 28 Sep 2024
Abstract
In recent years, flexible pressure sensors have garnered significant attention. However, the development of large-area, low-cost, and easily fabricated flexible pressure sensors remains challenging. We designed a flexible fiber optic pressure sensor for contact force detection based on the principle of backward Rayleigh [...] Read more.
In recent years, flexible pressure sensors have garnered significant attention. However, the development of large-area, low-cost, and easily fabricated flexible pressure sensors remains challenging. We designed a flexible fiber optic pressure sensor for contact force detection based on the principle of backward Rayleigh scattering using a single-mode optical fiber as the sensing element and polymer PDMS as the encapsulation material. To enhance the sensor’s sensitivity and stability, we optimized its structural design, parameters, and fabrication process and measured the fiber strain using an optical frequency domain reflectometer (OFDR). The results showed that the sensor achieved a high sensitivity of 6.93247 με/kPa with a PDMS concentration ratio of 10:1, a curing time of 2 h, and a substrate thickness of 5 mm. The sensor demonstrated excellent linearity and repeatability in static performance tests and was successfully used to monitor the plantar pressure distribution in real time. This flexible fiber optic pressure sensor can be developed via a simple fabrication process, has a low cost, and has high sensitivity, highlighting its potential applications in smart wearables and medical diagnostics. Full article
(This article belongs to the Special Issue Optical Sensors and Devices)
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Figure 1

Figure 1
<p>(<b>a</b>) Three-dimensional structure of the sensor; (<b>b</b>) physical drawing of the sensor.</p>
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<p>(<b>a</b>) Schematic diagram of the fiber optic structure; (<b>b</b>) physical drawing of the G.65A72.</p>
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<p>Schematic diagram of the sensor fabrication process.</p>
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<p>Working principle of OFDR.</p>
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<p>Test system.</p>
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<p>Stress–strain diagrams for PDMS substrates of different thicknesses.</p>
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<p>Stress–strain diagrams for different PDMS ratios.</p>
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<p>Stress–strain diagrams for different curing times.</p>
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<p>Relationship between the strain and pressure response of four-cycle loading.</p>
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<p>Relationship between the strain and pressure response of four loading/unloading cycles. (<b>a</b>) First loading/unloading cycle; (<b>b</b>) second loading/unloading cycle; (<b>c</b>) third loading/unloading cycle; (<b>d</b>) fourth loading/unloading cycle.</p>
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<p>(<b>a</b>) Pressure zoning of the soles of the feet; (<b>b</b>) insole-type fiber optic sensor structure.</p>
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<p>Test process.</p>
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<p>(<b>a</b>) Strain diagram of the output at static stand; (<b>b</b>) cloud view of distribution of plantar pressure during static standing.</p>
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<p>Gait analysis during walking: (<b>a</b>) heel on the ground; (<b>b</b>) full foot on the ground; (<b>c</b>) foot planted on the ground.</p>
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<p>The distribution of plantar pressure during walking: (<b>a</b>) heel on the ground; (<b>b</b>) full foot on the ground; (<b>c</b>) foot planted on the ground.</p>
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16 pages, 13027 KiB  
Article
A Real-Time Global Re-Localization Framework for a 3D LiDAR-Based Navigation System
by Ziqi Chai, Chao Liu and Zhenhua Xiong
Sensors 2024, 24(19), 6288; https://doi.org/10.3390/s24196288 (registering DOI) - 28 Sep 2024
Abstract
Place recognition is widely used to re-localize robots in pre-built point cloud maps for navigation. However, current place recognition methods can only be used to recognize previously visited places. Moreover, these methods are limited by the requirement of using the same types of [...] Read more.
Place recognition is widely used to re-localize robots in pre-built point cloud maps for navigation. However, current place recognition methods can only be used to recognize previously visited places. Moreover, these methods are limited by the requirement of using the same types of sensors in the re-localization process and the process is time consuming. In this paper, a template-matching-based global re-localization framework is proposed to address these challenges. The proposed framework includes an offline building stage and an online matching stage. In the offline stage, virtual LiDAR scans are densely resampled in the map and rotation-invariant descriptors can be extracted as templates. These templates are hierarchically clustered to build a template library. The map used to collect virtual LiDAR scans can be built either by the robot itself previously, or by other heterogeneous sensors. So, an important feature of the proposed framework is that it can be used in environments that have never been visited by the robot before. In the online stage, a cascade coarse-to-fine template matching method is proposed for efficient matching, considering both computational efficiency and accuracy. In the simulation with 100 K templates, the proposed framework achieves a 99% success rate and around 11 Hz matching speed when the re-localization error threshold is 1.0 m. In the validation on The Newer College Dataset with 40 K templates, it achieves a 94.67% success rate and around 7 Hz matching speed when the re-localization error threshold is 1.0 m. All the results show that the proposed framework has high accuracy, excellent efficiency, and the capability to achieve global re-localization in heterogeneous maps. Full article
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<p>The proposed global re-localization framework.</p>
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<p>Resampling in Gazebo using mesh model. (<b>Left</b>) AGV in Gazebo with mesh model, collecting point cloud data. (<b>Right</b>) Collected point cloud data.</p>
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<p>Extracted PCASC global descriptor (20 row × 60 column) from point cloud data in <a href="#sensors-24-06288-f002" class="html-fig">Figure 2</a>.</p>
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<p>The nearest-neighbor search engine building process.</p>
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<p>The online template matching procedure.</p>
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<p>Different clustering principles while merging clusters using 10 K samples. Clusters are identified from each other by color, where each dot represents a real sample, and representative templates for each cluster are plotted with black dots.</p>
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<p>Accuracy comparison between different number of candidates on simulated data.</p>
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<p>Efficiency comparison between different numbers of candidates on simulated data.</p>
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<p>The NCD dataset used for validation. (<b>a</b>) Top view of the test environment. Each test sample is plotted with a red dot at the location in the environment where it was collected. (<b>b</b>) Distribution of test samples on X-Y plane.</p>
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<p>Accuracy comparison between different numbers of candidates on real data.</p>
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<p>Efficiency comparison between different numbers of candidates on real data.</p>
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<p>Match result distribution between distance and similarity. (<b>a</b>) Exhaustive match result distribution. (<b>b</b>) LSH-KDT match result distribution.</p>
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<p>The change in candidate searching time with the increase in the number of representative templates for different <span class="html-italic">K</span> values.</p>
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16 pages, 5739 KiB  
Article
Comparison of IMU-Based Knee Kinematics with and without Harness Fixation against an Optical Marker-Based System
by Jana G. Weber, Ariana Ortigas-Vásquez, Adrian Sauer, Ingrid Dupraz, Michael Utz, Allan Maas and Thomas M. Grupp
Bioengineering 2024, 11(10), 976; https://doi.org/10.3390/bioengineering11100976 (registering DOI) - 28 Sep 2024
Abstract
The use of inertial measurement units (IMUs) as an alternative to optical marker-based systems has the potential to make gait analysis part of the clinical standard of care. Previously, an IMU-based system leveraging Rauch–Tung–Striebel smoothing to estimate knee angles was assessed using a [...] Read more.
The use of inertial measurement units (IMUs) as an alternative to optical marker-based systems has the potential to make gait analysis part of the clinical standard of care. Previously, an IMU-based system leveraging Rauch–Tung–Striebel smoothing to estimate knee angles was assessed using a six-degrees-of-freedom joint simulator. In a clinical setting, however, accurately measuring abduction/adduction and external/internal rotation of the knee joint is particularly challenging, especially in the presence of soft tissue artefacts. In this study, the in vivo IMU-based joint angles of 40 asymptomatic knees were assessed during level walking, under two distinct sensor placement configurations: (1) IMUs fixed to a rigid harness, and (2) IMUs mounted on the skin using elastic hook-and-loop bands (from here on referred to as “skin-mounted IMUs”). Estimates were compared against values obtained from a harness-mounted optical marker-based system. The comparison of these three sets of kinematic signals (IMUs on harness, IMUs on skin, and optical markers on harness) was performed before and after implementation of a REference FRame Alignment MEthod (REFRAME) to account for the effects of differences in coordinate system orientations. Prior to the implementation of REFRAME, in comparison to optical estimates, skin-mounted IMU-based angles displayed mean root-mean-square errors (RMSEs) up to 6.5°, while mean RMSEs for angles based on harness-mounted IMUs peaked at 5.1°. After REFRAME implementation, peak mean RMSEs were reduced to 4.1°, and 1.5°, respectively. The negligible differences between harness-mounted IMUs and the optical system after REFRAME revealed that the IMU-based system was capable of capturing the same underlying motion pattern as the optical reference. In contrast, obvious differences between the skin-mounted IMUs and the optical reference indicated that the use of a harness led to fundamentally different joint motion being measured, even after accounting for reference frame misalignments. Fluctuations in the kinematic signals associated with harness use suggested the rigid device oscillated upon heel strike, likely due to inertial effects from its additional mass. Our study proposes that optical systems can be successfully replaced by more cost-effective IMUs with similar accuracy, but further investigation (especially in vivo and upon heel strike) against moving videofluoroscopy is recommended. Full article
(This article belongs to the Special Issue Biomechanics of Human Movement and Its Clinical Applications)
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<p>The optical harness-based reference system, as well as two pairs of IMU sensors, were carefully positioned on each participant by a certified technician. One IMU pair was attached to the rigid harness of the reference system (“IMUs on harness”), and a second IMU pair was mounted on elastic hook-and-loop bands (“IMUs on skin”). As per the optical system manufacturer’s instructions, participants walked in socks on the treadmill.</p>
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<p>Mean tibiofemoral joint angles (solid lines) ± standard deviation (shaded areas), in degrees, as estimated by inertial measurement units (IMUs) on harness (purple), IMUs on skin (green), and optical motion capture (OMC) on harness (blue), averaged over all knees and cycles. Note that flexion angles have been illustrated as positive (despite representing a negative rotation around the laterally directed X-axis) for easier comparisons against other studies. Angles are shown as a percentage of the gait cycle under three conditions: (1) raw, i.e., in the absence of post-processing methods to correct reference frame orientation differences (<b>left</b>), (2) after implementation of REFRAME<sub><span class="html-italic">IMU</span>→<span class="html-italic">OMC</span></sub> (<b>middle</b>), and (3) after implementation of REFRAME<sub><span class="html-italic">RMS</span></sub> (<b>right</b>).</p>
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<p>Mean tibiofemoral joint angles (solid lines) ± standard deviation (shaded areas), in degrees, as estimated by inertial measurement units (IMUs) on harness (purple), IMUs on skin (green), and optical motion capture (OMC) on harness (blue), averaged over all cycles for knee 17. Note that flexion angles have been illustrated as positive (despite representing a negative rotation around the laterally directed X-axis) for easier comparisons against other studies. Angles are shown as a percentage of the gait cycle under three conditions: (1) raw, i.e., in the absence of post-processing methods to correct reference frame orientation differences (<b>left</b>), (2) after implementation of REFRAME<sub><span class="html-italic">IMU</span>→<span class="html-italic">OMC</span></sub> (<b>middle</b>), and (3) after implementation of REFRAME<sub><span class="html-italic">RMS</span></sub> (<b>right</b>).</p>
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<p>Mean ± standard deviation of root-mean-square errors (RMSEs, in degrees) between the optical reference system on a harness and the inertial measurement units on the harness (<b>left</b>), as well as between the optical reference system on a harness and the inertial measurement units on the skin (<b>right</b>). Shown for flexion/extension (<b>a</b>,<b>b</b>), abduction/adduction (<b>c</b>,<b>d</b>), and external/internal rotation (<b>e</b>,<b>f</b>). Significant changes in RMSEs after implementation of REFRAME<sub><span class="html-italic">IMU</span>→<span class="html-italic">OMC</span></sub> and of REFRAME<sub><span class="html-italic">RMS</span></sub>, as determined by paired <span class="html-italic">t</span>-tests, are shown (<span class="html-italic">p</span> &lt; 0.004 indicated by ***; full <span class="html-italic">p</span>-values are available in <a href="#app1-bioengineering-11-00976" class="html-app">Supplementary Materials Tables S121 and S122</a>).</p>
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<p>Mean ± standard deviation of root-mean-square errors (RMSEs, in degrees) between the optical reference system on a harness and the inertial measurement units on the harness (<b>left</b>), as well as between the optical reference system on a harness and the inertial measurement units on the skin (<b>right</b>). Shown for flexion/extension (<b>a</b>,<b>b</b>), abduction/adduction (<b>c</b>,<b>d</b>), and external/internal rotation (<b>e</b>,<b>f</b>). Significant changes in RMSEs after implementation of REFRAME<sub><span class="html-italic">IMU</span>→<span class="html-italic">OMC</span></sub> and of REFRAME<sub><span class="html-italic">RMS</span></sub>, as determined by paired <span class="html-italic">t</span>-tests, are shown (<span class="html-italic">p</span> &lt; 0.004 indicated by ***; full <span class="html-italic">p</span>-values are available in <a href="#app1-bioengineering-11-00976" class="html-app">Supplementary Materials Tables S121 and S122</a>).</p>
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24 pages, 8531 KiB  
Article
Acoustic Detection of Pipeline Blockages in Gas Extraction Systems: A Novel Approach
by Chun Liu, Zhongyi Man and Wenlong Li
Energies 2024, 17(19), 4875; https://doi.org/10.3390/en17194875 (registering DOI) - 28 Sep 2024
Abstract
Gas extraction is crucial for coal mine safety, yet pipeline blockages by solid slag and water severely hinder efficiency and pose risks. Traditional detection methods are limited by rapid signal attenuation and noise interference. In this study, an acoustic detection technology is introduced [...] Read more.
Gas extraction is crucial for coal mine safety, yet pipeline blockages by solid slag and water severely hinder efficiency and pose risks. Traditional detection methods are limited by rapid signal attenuation and noise interference. In this study, an acoustic detection technology is introduced for pipeline blockages, utilizing sensors at potential blockage points to collect sound wave data. Experiments with a scaled pipeline model reveal that slag blockages produce characteristic peaks in the 1200 Hz–2000 Hz range, while water blockages show peaks in the 1 kHz–2 kHz and 3.5 kHz–4.5 kHz bands. The longitudinal blockage intensity and extraction pressure significantly affect the sound pressure levels. A reliable fitting model predicts the blockage intensity based on acoustic signals, achieving high accuracy. This novel method enhances blockage identification, offering a non-invasive, cost-effective solution that improves coal mine safety and efficiency. Full article
(This article belongs to the Section H: Geo-Energy)
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<p>Experimental system in extraction pipelines and acoustic detection apparatus.</p>
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<p>Schematic diagram of a blocked pipeline section.</p>
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<p>Sound source signal characteristics under different extraction pressures.</p>
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<p>Sound source signal characteristics under different transverse blockage intensities.</p>
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<p>Sound source signal characteristics under different longitudinal blockage intensities.</p>
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<p>Sound source signal characteristics under different extraction negative pressures.</p>
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<p>Sound source signal characteristics under different transverse blockage intensity.</p>
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<p>Sound source signal characteristics under a different longitudinal blockage intensity.</p>
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<p>Average amplitude of the characteristic frequency band under different extraction pressures.</p>
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<p>Average amplitude of the characteristic frequency band under different transverse blockage intensities.</p>
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<p>Average amplitude of the characteristic frequency band under different longitudinal blockage intensities.</p>
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<p>Fitting curve of average sound pressure level in characteristic frequency band under extraction pressures and longitudinal blockage intensities.</p>
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<p>Average amplitude of the characteristic frequency band under different extraction pressures. (<b>a</b>) Average amplitude of the characteristic frequency band 1 (1 kHz~2 kHz). (<b>b</b>) Average amplitude of the characteristic frequency band 2 (3.5 kHz~4.5 kHz).</p>
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<p>Average amplitude of the characteristic frequency band under different transverse blockage intensities. (<b>a</b>) Average amplitude of the characteristic frequency band 1 (1 kHz~2 kHz). (<b>b</b>) Average amplitude of the characteristic frequency band 2 (3.5 kHz~4.5 kHz).</p>
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<p>Average amplitude of characteristic frequency band under different longitudinal blockage intensities. (<b>a</b>) Average amplitude of the characteristic frequency band 1 (1 kHz~2 kHz). (<b>b</b>) Average amplitude of the characteristic frequency band 2 (3.5 kHz~4.5 kHz).</p>
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<p>Fitting curve of average sound pressure level in characteristic frequency band 1.</p>
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13 pages, 3334 KiB  
Article
Gelatin-Coated High-Sensitivity Microwave Sensor for Humidity-Sensing Applications
by Junho Yeo and Younghwan Kwon
Sensors 2024, 24(19), 6286; https://doi.org/10.3390/s24196286 (registering DOI) - 28 Sep 2024
Abstract
In this paper, the humidity-sensing characteristics of gelatin were compared with those of poly(vinyl alcohol) (PVA) at L-band (1 ~ 2 GHz) microwave frequencies. A capacitive microwave sensor based on a defected ground structure with a modified interdigital capacitor (DGS-MIDC) in a microstrip [...] Read more.
In this paper, the humidity-sensing characteristics of gelatin were compared with those of poly(vinyl alcohol) (PVA) at L-band (1 ~ 2 GHz) microwave frequencies. A capacitive microwave sensor based on a defected ground structure with a modified interdigital capacitor (DGS-MIDC) in a microstrip transmission line operating at 1.5 GHz without any coating was used. Gelatin is a natural polymer based on protein sourced from animal collagen, whereas PVA is a high-sensitivity hydrophilic polymer that is widely used for humidity sensors and has a good film-forming property. Two DGS-MIDC-based microwave sensors coated with type A gelatin and PVA, respectively, with a thickness of 0.02 mm were fabricated. The percent relative frequency shift (PRFS) and percent relative magnitude shift (PRMS) based on the changes in the resonant frequency and magnitude level of the transmission coefficient for the microwave sensor were used to compare the humidity-sensing characteristics. The relative humidity (RH) was varied from 50% to 80% with a step of 10% at a fixed temperature of around 25 °C using a low-reflective temperature and humidity chamber manufactured with Styrofoam. The experiment’s results show that the capacitive humidity sensitivity of the gelatin-coated microwave sensor in terms of the PRFS and PRMS was higher compared to that of the PVA-coated one. In particular, the sensitivity of the gelatin-coated microwave sensor at a low RH from 50% to 60% was much greater compared to that of the PVA-coated one. In addition, the relative permittivity of the fabricated microwave sensors coated with PVA and gelatin was extracted by using the measured PRFS and the equation was derived by curve-fitting the simulated results. The change in the extracted relative permittivity for the gelatin-coated microwave sensor was larger than that of the PVA-coated one for varying the RH. Full article
(This article belongs to the Special Issue RF and IoT Sensors: Design, Optimization and Applications)
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<p>Chemical structures of (<b>a</b>) PVA and (<b>b</b>) gelatin.</p>
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<p>DGS-MIDC-based microwave sensor: (<b>a</b>) geometry, (<b>b</b>) electric-field distribution at 1.5 GHz, and (<b>c</b>) S-parameter characteristics and simplified equivalent circuit model.</p>
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<p>Performance characteristics of the DGS-MIDC-based microwave sensor for varying relative permittivity of the coated polymer with tan <span class="html-italic">δ</span> = 0: (<b>a</b>) S<sub>21</sub>, (<b>b</b>) <span class="html-italic">f</span><sub>r</sub>, and (<b>c</b>) PRFS.</p>
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<p>Extracted equivalent circuit parameters of the DGS-MIDC-based microwave sensor for varying relative permittivity of the coated polymer with tan <span class="html-italic">δ</span> = 0: (<b>a</b>) <span class="html-italic">C</span><sub>1</sub>; (<b>b</b>) <span class="html-italic">L</span><sub>1</sub>; (<b>c</b>) Δ<span class="html-italic">C</span><sub>1</sub>/<span class="html-italic">C</span><sub>1</sub>(%) and (<b>d</b>) Δ<span class="html-italic">L</span><sub>1</sub>/<span class="html-italic">L</span><sub>1</sub>(%).</p>
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<p>Photographs of the fabricated microwave sensors coated with (<b>a</b>) PVA and (<b>b</b>) gelatin.</p>
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<p>Block diagram and photographs of the experiment setup for the humidity-sensing measurements: (<b>a</b>) block diagram, (<b>b</b>) experiment setup with an open-top cover, and (<b>c</b>) experiment setup with closed-top cover.</p>
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<p>Measured S<sub>21</sub> characteristics of the fabricated microwave sensors coated with the polymers for varying RH. (<b>a</b>) PVA and (<b>b</b>) gelatin.</p>
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<p>Performance comparison of the fabricated microwave sensors coated with the polymers for varying RH. (<b>a</b>) <span class="html-italic">f</span><sub>r</sub>, (<b>b</b>) PRFS, (<b>c</b>) S<sub>21</sub> magnitude, and (<b>d</b>) PRMS.</p>
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<p>Comparison of the extracted relative permittivity from measured PRFSs of PVA- and gelatin-coated microwave sensors for varying RH.</p>
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12 pages, 1510 KiB  
Article
Evaluation of Hand Muscle Strength Using Manual Dynamometry: A Reliability and Validity Study of the Activ5 Instrument
by José Pino-Ortega, Rafael Carvajal-Espinoza and Boryi A. Becerra-Patiño
Appl. Sci. 2024, 14(19), 8775; https://doi.org/10.3390/app14198775 (registering DOI) - 28 Sep 2024
Abstract
Manual dynamometry (HHD) allows for the assessment of musculature because its use has been supported as an indicator of health in different population groups. The objective of this study was to examine the reliability and validity of the Activ5 dynamometer for assessing grip [...] Read more.
Manual dynamometry (HHD) allows for the assessment of musculature because its use has been supported as an indicator of health in different population groups. The objective of this study was to examine the reliability and validity of the Activ5 dynamometer for assessing grip strength in a population of adults. A total of 106 individuals with an age of 20.38 ± 1.64, body mass of 71.52 ± 11.32 kg, and height of 1.70 ± 0.11 m were evaluated during two sessions. A cross-sectional agreement study was conducted on Sports Science students from a university community, and 106 individuals were evaluated during two sessions. Statistical analysis of reliability and validity was performed using intraclass correlation coefficients (ICCs), Pearson correlations, and Lin’s coefficient. According to Lin’s coefficient, both instruments measure grip strength for both conditions, either for the right hand or the left hand. The correlation coefficient to determine the linear relationship between both instruments determined that between the Jamar right-hand dynamometer and the right-hand Activ5, a coefficient R2 = 0.580, p = 0.00, was obtained. In contrast, the correlation between the Jamar left-hand dynamometer and the left-hand Activ5 had a coefficient R2 = 0.543, p = 0.001. Both intraclass correlation coefficients and Cronbach’s alpha presented high values, indicating that both instruments have good reproducibility in their measurements. The Activ5 dynamometer cannot be used interchangeably with the Jamar dynamometer; however, the close values reported make it a reliable tool in grip strength assessment. The different characteristics of the Activ5 instrument, such as its ergonomics, weight, portability, wireless connection, dimensions, and applications, make it a promising daily- use tool for assessing, monitoring, and the prescription of physical activity and exercise. Full article
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<p>Activ5 force dynamometer. Source: <a href="https://activ5.com/" target="_blank">https://activ5.com/</a>.</p>
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<p>Correlation between Jamar dynamometer and Activ5 for the right hand.</p>
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<p>Correlation between Jamar dynamometer and Activ5 for the left hand. <b>Note:</b> The gray points are the measurements of each of the pairs of measurements, it is a scatter plot, so each pair of measurements of each variable for each data. The dashed line is the trend line that graphically shows the relationship between the variables. If the line is increasing, the relationship is positive.</p>
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<p>Bland–Altman plot of the differences for the right hand.</p>
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<p>Bland–Altman plot of the differences for the left hand.</p>
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31 pages, 2325 KiB  
Review
ML-Based Maintenance and Control Process Analysis, Simulation, and Automation—A Review
by Izabela Rojek, Dariusz Mikołajewski, Ewa Dostatni, Adrianna Piszcz and Krzysztof Galas
Appl. Sci. 2024, 14(19), 8774; https://doi.org/10.3390/app14198774 (registering DOI) - 28 Sep 2024
Abstract
Automation and digitalization in various industries towards the Industry 4.0/5.0 paradigms are rapidly progressing thanks to the use of sensors, Industrial Internet of Things (IIoT), and advanced fifth generation (5G) and sixth generation (6G) mobile networks supported by simulation and automation of processes [...] Read more.
Automation and digitalization in various industries towards the Industry 4.0/5.0 paradigms are rapidly progressing thanks to the use of sensors, Industrial Internet of Things (IIoT), and advanced fifth generation (5G) and sixth generation (6G) mobile networks supported by simulation and automation of processes using artificial intelligence (AI) and machine learning (ML). Ensuring the continuity of operations under different conditions is becoming a key factor. One of the most frequently requested solutions is currently predictive maintenance, i.e., the simulation and automation of maintenance processes based on ML. This article aims to extract the main trends in the area of ML-based predictive maintenance present in studies and publications, critically evaluate and compare them, and define priorities for their research and development based on our own experience and a literature review. We provide examples of how BCI-controlled predictive maintenance due to brain–computer interfaces (BCIs) play a transformative role in AI-based predictive maintenance, enabling direct human interaction with complex systems. Full article
(This article belongs to the Special Issue Automation and Digitization in Industry: Advances and Applications)
13 pages, 9028 KiB  
Article
Rapid Real-Time Prediction Techniques for Ammonia and Nitrite in High-Density Shrimp Farming in Recirculating Aquaculture Systems
by Fudi Chen, Tianlong Qiu, Jianping Xu, Jiawei Zhang, Yishuai Du, Yan Duan, Yihao Zeng, Li Zhou, Jianming Sun and Ming Sun
Fishes 2024, 9(10), 386; https://doi.org/10.3390/fishes9100386 (registering DOI) - 28 Sep 2024
Abstract
Water quality early warning is a key aspect in industrial recirculating aquaculture systems for high-density shrimp farming. The concentrations of ammonia nitrogen and nitrite in the water significantly impact the cultured animals and are challenging to measure in real-time, posing a substantial challenge [...] Read more.
Water quality early warning is a key aspect in industrial recirculating aquaculture systems for high-density shrimp farming. The concentrations of ammonia nitrogen and nitrite in the water significantly impact the cultured animals and are challenging to measure in real-time, posing a substantial challenge to water quality early warning technology. This study aims to collect data samples using low-cost water quality sensors during the industrial recirculating aquaculture process and to construct predictive values for ammonia nitrogen and nitrite, which are difficult to obtain through sensors in the aquaculture environment, using data prediction techniques. This study employs various machine learning algorithms, including General Regression Neural Network (GRNN), Deep Belief Network (DBN), Long Short-Term Memory (LSTM), and Support Vector Machine (SVM), to build predictive models for ammonia nitrogen and nitrite. The accuracy of the models is determined by comparing the predicted values with the actual values, and the performance of the models is evaluated using Mean Absolute Error (MAE), Mean Absolute Percentage Error (MAPE), and Root Mean Square Error (RMSE) metrics. Ultimately, the optimized GRNN-based predictive model for ammonia nitrogen concentration (MAE = 0.5915, MAPE = 28.95%, RMSE = 0.7765) and the nitrite concentration predictive model (MAE = 0.1191, MAPE = 29.65%, RMSE = 0.1904) were selected. The models can be integrated into an Internet of Things system to analyze the changes in ammonia nitrogen and nitrite concentrations over time through aquaculture management and routine water quality conditions, thereby achieving the application of recirculating aquaculture system water environment early warning technology. Full article
(This article belongs to the Special Issue Advances in Recirculating and Sustainable Aquaculture Systems)
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<p>The experimental RAS: (<b>A</b>) the schematic of the image acquisition system; (<b>B</b>) the high-density shrimp RAS in Dalian Huixin Titanium Equipment Development Co., Ltd. (Dalian, China).</p>
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<p>Artificial Neural Network Algorithm Structure Diagram: (<b>A</b>) Classic artificial neural network structure; (<b>B</b>) LSTM structure diagram.</p>
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<p>Results of TAN predicting model based on the training data.</p>
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<p>Results of TAN predicting model based on the testing data.</p>
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<p>Results of nitrite nitrogen predicting model based on the training data.</p>
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<p>Results of nitrite nitrogen predicting model based on the testing data.</p>
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<p>(<b>a</b>–<b>h</b>) Scatter plot distribution of TAN prediction data for GRNN, LSTM, DBN, and SVM models.</p>
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<p>(<b>a</b>–<b>h</b>) Scatter plot distribution of NO<sub>2</sub>-N prediction data for GRNN, LSTM, DBN, and SVM models.</p>
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26 pages, 6512 KiB  
Article
p66Shc Protein—Oxidative Stress Sensor or Redox Enzyme: Its Potential Role in Mitochondrial Metabolism of Human Breast Cancer
by Monika Prill, Vilma A. Sardão, Mateusz Sobczak, Dominika Nowis, Jedrzej Szymanski and Mariusz R. Wieckowski
Cancers 2024, 16(19), 3324; https://doi.org/10.3390/cancers16193324 (registering DOI) - 28 Sep 2024
Abstract
This work presents a comprehensive evaluation of the role of p66Shc protein in mitochondrial physiology in MDA-MB-231 breast cancer cells. The use of human breast cancer cell line MDA-MB-231 and its genetically modified clones (obtained with the use of the CRISPR-Cas9 technique), expressing [...] Read more.
This work presents a comprehensive evaluation of the role of p66Shc protein in mitochondrial physiology in MDA-MB-231 breast cancer cells. The use of human breast cancer cell line MDA-MB-231 and its genetically modified clones (obtained with the use of the CRISPR-Cas9 technique), expressing different levels of p66Shc protein, allowed us to demonstrate how the p66Shc protein affects mitochondrial metabolism of human breast cancer cells. Changes in the level of p66Shc (its overexpression, and overexpressing of its Serine 36-mutated version, as well as the knockout of p66Shc) exert different effects in breast cancer cells. Interestingly, knocking out p66Shc caused significant changes observed mostly in mitochondrial bioenergetic parameters. We have shown that an MDA-MB-231 (which is a strong metastatic type of breast cancer) clone lacking p66Shc protein is characterized by a significant shift in the metabolic phenotype in comparison to other MDA-MB-231 clones. Additionally, this clone is significantly more vulnerable to doxorubicin treatment. We have proved that p66Shc adaptor protein in human breast cancer cells may exert a different role than in noncancerous cells (e.g., fibroblasts). Full article
(This article belongs to the Special Issue Emerging Insights into Cell Death in Cancer)
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<p>Characteristics of ShcA family protein. (<b>A</b>) Domain structure of ShcA isoforms. (<b>B</b>) Pro-oxidative signaling pathway of p66Shc isoform. PKCβ—protein kinase Cβ; S660-PKCβ—phosphorylated in S660 form of protein kinase Cβ; Pin1—prolyl isomerase; PP2A—serine/threonine protein phosphatase type 2; cyt. c—cytochrome c.</p>
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<p>The level of ShcA proteins (p66Shc, p52Shs, and p46Shc) in the MDA-MB-231 clones obtained through (<b>A</b>) lentiviral transfection or (<b>B</b>) genome editing using the CRISPR/Cas9 method by the Western Blot analysis. (<b>C</b>) The schematic of the U6gRNA-Cas9-2A-GFP plasmid, enabling genome editing in MDA-MB-231 cell lines. (<b>D</b>) Proliferation rates of the individual MDA-MB-231 clones.</p>
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<p>The level of individual proteins involved in the pro-oxidative p66Shc signaling pathway. The levels of proteins were standardized to the GAPDH ± SD. ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>The status of ROS homeostasis. (<b>A</b>) The level of ROS species (measured with the use of redox-sensitive fluorophore CM-H<sub>2</sub>DCF-DA, DHE, and MitoSOX, respectively); (<b>B</b>) the level of antioxidant enzymes evaluated by WB; the levels of individual antioxidant enzymes were standardized to the GAPDH ± SD; (<b>C</b>) the mass spectrometry (MS)–proteomic analysis of antioxidant enzymes in the MDA-MB-231 clones. Samples were standardized to the Ctrl (clone with empty vector). A blue color represents a decrease, while a red color represents an increase in the protein levels; ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001; the levels of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) as well as mitochondrial and cytosolic superoxide are shown as a percent of the control value.</p>
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<p>Mitochondrial bioenergetics parameters in the MDA-MB-231 clones. (<b>A</b>) Mitochondrial membrane potential and selected functional parameters of the mitochondrial respiratory chain: the (<b>B</b>) basal respiration, (<b>C</b>) respiration associated with ATP synthesis, (<b>D</b>) proton leak, (<b>E</b>) maximal respiration in the presence of CCCP. The oxygen consumption rate was calculated as pmolO<sub>2</sub> per sec per million of cells. ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>The mass spectrometry (MS)–proteomic analysis of the levels of individual subunits of the OXPHOS in MDA-MB-231 clones. (<b>A</b>) Complex I, (<b>B</b>) complex II, (<b>C</b>) complex III, (<b>D</b>) complex IV, and (<b>E</b>) ATP synthase. Samples were standardized to the control clone (Ctrl). A blue color represents a decrease, while a red color represents an increase in the protein levels.</p>
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<p>The mass spectrometry (MS)–proteomic analysis of individual enzymes involved in the (<b>A</b>) Krebs cycle as well as (<b>B</b>) glycolysis determined in clones of the MDA-MB-231. A blue color represents a decrease, while a red color represents an increase in the protein levels.</p>
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<p>Assessment of cell death (apoptosis) levels in the individual clones (−/+ DOX) of the MDA-MB-231 cell line. (<b>A</b>) Representative scatter plots showing changes in annexin V and PI fluorescence intensity. The determination of the percentage of live cells (annexin V−/PI−; lower left quadrant), early apoptotic cells (annexin V+/PI−; lower right quadrant), and late apoptotic cells (annexin V+/PI+; upper right quadrant) in the individual clones of MDA-MB-231 cells (−) DOX and (+) DOX; bar graphs showing the percentage of different cell groups: (<b>B</b>) live cells, (<b>C</b>) early apoptotic cells, and (<b>D</b>) late apoptotic cells; * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01; *** <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>Mitochondrial network morphology in the individual MDA-MB-231 clones treated (+DOX) and untreated (–DOX) with doxorubicin and mitochondrial membrane potential. The (<b>A</b>) Ctrl, (<b>B</b>) clone with elevated p66Shc protein levels, (<b>C</b>) clone with elevated levels of the S36A-mutated form of the p66Shc protein, and (<b>D</b>) clone with the knockout of p66Shc; nuclei were stained blue (DAPI) and the mitochondrial network was stained red (MitoRed); (<b>E</b>) mitochondrial membrane potential, * <span class="html-italic">p</span> &lt; 0.05.</p>
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<p>The characteristics of the energetic profile of individual clones of MDA-MB-231 after doxorubicin treatment. (<b>A</b>) The proton efflux rate (PER) profile (extracellular acidification profile) for MDA-MB-231 clones untreated and treated with DOX; (<b>B</b>) the percentage contribution of “PER from glycolysis” in clones treated and untreated with DOX presented as a bar graph and a pie chart: * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01; (<b>C</b>) the metabolic phenotype of MDA-MB-231 clones after DOX treatment and (<b>D</b>) individual clones (−DOX) and (+DOX), respectively: * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>Basal oxygen consumption rate in individual clones of MDA-MB-231 after doxorubicin treatment; * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01.</p>
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