Nothing Special   »   [go: up one dir, main page]

You seem to have javascript disabled. Please note that many of the page functionalities won't work as expected without javascript enabled.
 
 
Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline
remove_circle_outline

Journals

Article Types

Countries / Regions

Search Results (1)

Search Parameters:
Keywords = nested-PCR/RLFP

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
11 pages, 2269 KiB  
Article
Survey for ‘Candidatus Liberibacter’ and ‘Candidatus Phytoplasma’ in Citrus in Chile
by Nicolas Quiroga, Camila Gamboa, Gabriela Medina, Nicoletta Contaldo, Fernando Torres, Assunta Bertaccini, Alan Zamorano and Nicola Fiore
Pathogens 2022, 11(1), 48; https://doi.org/10.3390/pathogens11010048 - 31 Dec 2021
Cited by 4 | Viewed by 2572
Abstract
The considerable economic losses in citrus associated with ‘Candidatus Liberibacter’ and ‘Candidatus Phytoplasma’ presence have alerted all producing regions of the world. In Chile, none of these bacteria have been reported in citrus species. During the years 2017 and 2019, 258 [...] Read more.
The considerable economic losses in citrus associated with ‘Candidatus Liberibacter’ and ‘Candidatus Phytoplasma’ presence have alerted all producing regions of the world. In Chile, none of these bacteria have been reported in citrus species. During the years 2017 and 2019, 258 samples presenting symptoms similar to those associated with the presence of these bacteria were examined. No detection of ‘Ca. Liberibacter’ associated with “huanglongbing” disease was obtained in the tested samples; therefore, this quarantine pest is maintained as absent in Chile. However, 14 plants resulted positive for phytoplasmas enclosed in subgroups 16SrV-A (12 plants) and 16SrXIII-F (2 plants). Although they have been found in other plant species, this is the first report of these phytoplasmas in citrus worldwide. Full article
(This article belongs to the Section Bacterial Pathogens)
Show Figures

Figure 1

Figure 1
<p>Phylogenetic tree of (<b>A</b>) 16S rRNA gene region (1240 nt) and (<b>B</b>) <span class="html-italic">Ssu12p</span> region (724 nt) enclosing phytoplasmas detected in citrus from Chile, highlighted with filled diamonds, and selected ‘<span class="html-italic">Ca</span>. Phytoplasma’ strains. Information on the phytoplasma strains is reported in <a href="#pathogens-11-00048-t001" class="html-table">Table 1</a>. The tree was constructed using the maximum parsimony algorithm. The numbers in the nodes represent starting values based on 500 pseudo-replications for stability estimation and clade support <span class="html-italic">A. laidlawii</span> is used as outgroup strain.</p>
Full article ">Figure 2
<p>In silico RFLP profiles of the phytoplasma strains in the 16SrV subgroups. (<b>A</b>) Restriction profiles generated by the <span class="html-italic">Rsa</span>I enzyme. (<b>B</b>) Restriction profiles generated by the <span class="html-italic">Bfa</span>I enzyme. CTC 192 is a citrus sample. MW: molecular marker PhiX174 digested with <span class="html-italic">HaeII</span>I. Fragment size (nt) from top to bottom: 1353, 1078, 872, 603, 310, 281, 271, 234, 194, 118, and 72. Phytoplasmas used for comparison are: 16SrV-A, elm yellows (EY) ‘<span class="html-italic">Ca</span>. P. ulmi ‘(GenBank accession number: AY197655); 16SrV-B, jujube witches’ broom (JWB-G1) ‘<span class="html-italic">Ca</span>. P. ziziphi ‘(GenBank accession number: AB052876); 16SrV-C, “flavescence dorée” (FD-C) (GenBank accession number: X76560); 16SrV-D, “flavescence dorée” (FD-D) (GenBank accession number: AJ548787); 16SrV-E, rubus stunt (RuS) ‘<span class="html-italic">Ca</span>. P. rubi ‘(GenBank accession number: AY197648); 16SrV-G, Korean jujube witches’ broom (GenBank accession number: AB052879).</p>
Full article ">Figure 3
<p>In silico RFLP profiles of phytoplasmas in the subgroups of 16SrXIII ribosomal group. (<b>A</b>) Restriction profiles generated by the <span class="html-italic">Kpn</span>I enzyme. (<b>B</b>) Restriction profiles generated by the <span class="html-italic">Rsa</span>I enzyme. CTC 192 citrus sample. MW: molecular marker PhiX174 digested with <span class="html-italic">Hae</span>III. Fragment size (nt) from top to bottom: 1353, 1078, 872, 603, 310, 281, 271, 234, 194, 118, and 72. Phytoplasmas used for comparison: 16SrXIII-A, Mexican periwinkle virescence (MPV) ‘<span class="html-italic">Ca</span>. P. hispanicum’ (GenBank accession number: AF248960); 16SrXIII-B, strawberry green petal (STRAWB2) (GenBank accession number: U96616); 16SrXIII-C, Chinaberry yellows (CBY1) (GenBank accession number: AF495882); 16SrXIII-D, Mexican potato purple top (SINPV) (GenBank accession number: FJ914647); 16SrXIII-E, papaya apical curl necrosis (PACN) (GenBank accession number: EU719111); 16SrXIII-F strawberry red leaf (GenBank accession number: KJ921641); 16SrXIII-G, Chinaberry yellowing (ChTY) ‘<span class="html-italic">Ca</span>. P. meliae ‘(GenBank accession number: KU850940); 16SrXIII-H broccoli stunt phytoplasma (GenBank accession number: JX626329); 16SrXIII-I Mexican periwinkle virescence phytoplasma (GenBank accession number: KT444664).</p>
Full article ">Figure 4
<p>Symptoms of leaf yellowing in the citrus samples resulted positive for 16SrV-A phytoplasmas. (<b>A</b>) Orange CTC 192. (<b>B</b>) Lemon CTC 199. (<b>C</b>) Mandarin CTC 200. (<b>D</b>) Orange CTC 202.</p>
Full article ">Figure 5
<p>Symptoms in citrus associated with the 16SrXIII-F phytoplasma presence. (<b>A</b>) CTC 170, orange plant with generalized yellowing and untimely flowering. (<b>B</b>) CTC 134, mandarin plant with threadlike leaves and witches’ broom.</p>
Full article ">
Back to TopTop