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22 pages, 2602 KiB  
Article
Understanding and Exploring the Food Preferences of Filipino School-Aged Children Through Free Drawing as a Projective Technique
by Melvin Bernardino, Nicole Kate Diaz Sison, Jeanne Carla Bruce, Claudio Tiribelli and Natalia Rosso
Nutrients 2024, 16(23), 4035; https://doi.org/10.3390/nu16234035 - 25 Nov 2024
Abstract
Background and Objectives: Numerous traditional and innovative approaches have been employed to understand and evaluate children’s food preferences and food and nutrition knowledge, recognizing their essential role in shaping good nutrition. Drawing as a projective technique allows children to express their unconscious thoughts [...] Read more.
Background and Objectives: Numerous traditional and innovative approaches have been employed to understand and evaluate children’s food preferences and food and nutrition knowledge, recognizing their essential role in shaping good nutrition. Drawing as a projective technique allows children to express their unconscious thoughts and preferences through visual representation, distinguishing it from other methods by providing an insight into their inner feelings and perceptions that may not be easily articulated through verbal techniques. The main goals of the study are to use drawing as a projective technique to gain insights into children’s food preferences, and to examine the children’s current nutrition knowledge and dietary perceptions. Methods: This study involved school-aged children from four public schools in San Jose City, Nueva Ecija, Philippines, who met the inclusion criteria and provided parental consent and the children’s permission. Data collection included (a) questionnaires to measure food group and food frequency knowledge, the children’s opinions on food healthiness and likability, and (b) a drawing activity as a projective technique. The questionnaire scores and the specific foods on the children’s drawings were entered into an electronic worksheet and analyzed quantitatively. Results: The majority of Filipino school-aged children have a low (50%) to average (43%) level of food group knowledge and an average (62%) to low (32%) level of food frequency knowledge. The children can identify the healthiness of the food, but they express a liking for both healthy and unhealthy options. The children’s drawings showed a low preference for Glow food groups, including fruits and vegetables (47%), as compared to Grow foods (94%), Beverages (89%), and Go foods (85%) groups. “Rice and Egg”, the most paired items, indicated a preference among Filipino children. Gender-based analysis showed girls favored “Ice Cream”, “Bread”, “Apple”, and “Oranges” more than boys, but there were no significant gender differences found in Grow food group preferences. Conclusions: Children’s drawings are an effective, valuable complementary tool for understanding children’s food preferences, displaying the value of creative methods in gaining unique insights. The results highlight specific gaps in knowledge, such as the need for a better understanding of food groups and the importance of fruits and vegetables among children. Addressing these gaps in educational programs could enhance children’s food knowledge and encourage healthier dietary choices. Nutrition education programs might use interactive activities focused on food groups and emphasize the benefits of fruits and vegetables to promote better dietary habits for the improvement of children’s long-term health outcomes. Full article
(This article belongs to the Section Nutritional Policies and Education for Health Promotion)
16 pages, 2449 KiB  
Article
Identification of Cherry Tomato Volatiles Using Different Electron Ionization Energy Levels
by Dalma Radványi, László Csambalik, Dorina Szakál and Attila Gere
Molecules 2024, 29(23), 5567; https://doi.org/10.3390/molecules29235567 - 25 Nov 2024
Abstract
A comprehensive analysis of the volatile components of 11 different cherry tomato pastes (Tesco Extra, Orange, Zebra, Yellow, Round Netherland, Mini San Marzano, Spar truss, Tesco Sunstream, Paprikakertész, Mc Dreamy, and Tesco Eat Fresh) commercially available in Hungary was performed. In order to [...] Read more.
A comprehensive analysis of the volatile components of 11 different cherry tomato pastes (Tesco Extra, Orange, Zebra, Yellow, Round Netherland, Mini San Marzano, Spar truss, Tesco Sunstream, Paprikakertész, Mc Dreamy, and Tesco Eat Fresh) commercially available in Hungary was performed. In order to ensure the reliability and accuracy of the measurement, the optimal measurement conditions were first determined. SPME (solid-phase microextraction) fiber coating, cherry tomato paste treatment, and SPME sampling time and temperature were optimized. CAR/PDMS (carboxen/polydimethylsiloxane) fiber coating with a film thickness of 85 µm is suggested at a 60 °C sampling temperature and 30 min extraction time. A total of 64 common compounds was found in the prepared, mashed cherry tomato samples, in which 59 compounds were successfully identified. Besides the already published compounds, new, cherry tomato-related compounds were found, such as 3 methyl 2 butenal, heptenal, Z-4-heptenal, E-2-heptenal, E-carveol, verbenol, limonene oxide, 2-decen-1-ol, Z-4-decen-1-al, caryophyllene oxide, and E,E-2,4-dodecadienal. Supervised and unsupervised classification methods have been used to classify the tomato varieties based on their volatiles, which identified 16 key components that enable the discrimination of the samples with a high accuracy. Full article
(This article belongs to the Special Issue Extraction and Analysis of Natural Products in Food—2nd Edition)
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<p>(<b>a</b>) Comparison of SPME fiber coatings in the case of captured tomato volatiles. (<b>b</b>) Examination of the effect of seven different extraction temperatures (10, 25, 30, 35, 50, 60, and 80 min) on captured VOCs from tomatoes. (<b>c</b>) Examination of the effect of six different extraction times (10, 20, 30, 40, 60, and 120 min) on captured VOCs from tomatoes. (<b>d</b>) Evaluation of the effect of solvent addition (methanol and water).</p>
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<p>Mass spectra of hexanal at 70 eV, 60 eV, 50 eV, 40 eV, 20 eV, 10 eV, and 5 eV.</p>
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<p>Mass spectra of 2H-1b,4-Ethanopentaleno[1,2-b]oxirene,hexahydro-(1aa,1bb,4b,4aa,5aa) at 70 eV and 5 eV. Match factor: 92.8%, structure: C<sub>10</sub>H<sub>14</sub>O, molecular weight: 150.1, and CAS number: 117221-80-4. TIC: total ion chromatogram.</p>
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<p>Agglomerative hierarchical clustering using the Euclidean distance and Ward’s method. Dashed line represents an arbitrary cut of the dendrogram. Colors and numbers represent the mashed tomato samples (1: Tesco Extra, 2: Orange, 3: Zebra, 4: Yellow, 5: Round Netherland, 6: Mini San Marzano, 7: Spar truss, 8: Tesco Sunstream, 9: Paprikakertész, 10: Mc Dreamy, and a11: Tesco Eat Fresh), while letters represent the repetitions (a, b, and c).</p>
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<p>The discriminant analysis runs on principal component analysis loadings. The first discriminant function accounts for 97.76% of variance. Colors and numbers represent the mashed tomato samples (1: Tesco Extra, 2: Orange, 3: Zebra, 4: Yellow, 5: Round Netherland, 6: Mini San Marzano, 7: Spar truss, 8: Tesco Sunstream, 9: Paprikakertész, 10: Mc Dreamy, and 11: Tesco Eat Fresh), while letters represent the repetitions (a, b, and c). D: discriminant function.</p>
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30 pages, 13659 KiB  
Article
Spatial Gap-Filling of Himawari-8 Hourly AOD Products Using Machine Learning with Model-Based AOD and Meteorological Data: A Focus on the Korean Peninsula
by Youjeong Youn, Seoyeon Kim, Seung Hee Kim and Yangwon Lee
Remote Sens. 2024, 16(23), 4400; https://doi.org/10.3390/rs16234400 - 25 Nov 2024
Viewed by 189
Abstract
Given the complex spatiotemporal variability of aerosols, high-frequency satellite observations are essential for accurately mapping their distribution. However, optical remote sensing encounters difficulties in detecting Aerosol Optical Depth (AOD) over cloud-covered regions, creating data gaps that limit comprehensive environmental analysis. This study introduces [...] Read more.
Given the complex spatiotemporal variability of aerosols, high-frequency satellite observations are essential for accurately mapping their distribution. However, optical remote sensing encounters difficulties in detecting Aerosol Optical Depth (AOD) over cloud-covered regions, creating data gaps that limit comprehensive environmental analysis. This study introduces a spatial gap-filling method for Himawari-8/Advanced Himawari Imager (AHI) hourly AOD data, using a Random Forest (RF) model that integrates meteorological variables and model-based AOD data. Developed and validated over South Korea from 1 January to 31 December 2019, the model effectively improved data coverage from 6% to 100%. The approach demonstrated high performance in blind tests, achieving a root mean square error (RMSE) of 0.064 and a correlation coefficient (CC) of 0.966. Meteorological analysis indicated optimal model performance under cold, dry conditions (RMSE: 0.047, CC: 0.956), compared to humid conditions (RMSE: 0.105, CC: 0.921). Validation against Aerosol Robotic Network (AERONET) ground observations showed that, while the original Himawari-8 data exhibited higher accuracy (RMSE: 0.189, CC: 0.815, n = 346), the gap-filled dataset maintained reasonable precision (RMSE: 0.208, CC: 0.711) and significantly increased the number of valid data points (n = 4149). Furthermore, the gap-filled dataset successfully captured seasonal AOD patterns, with values ranging from 0.245–0.300 in winter to 0.381–0.391 in summer, providing a comprehensive view of aerosol dynamics across South Korea. Full article
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<p>Examples of converting 3-hourly to hourly data using cubic spline interpolation for meteorological variables: (<b>a</b>) before and after temporal interpolation for the TMP on 1 March 2019 and (<b>b</b>) before and after temporal interpolation for the RH on 1 March 2019. The top row in each panel (<b>a</b>,<b>b</b>) shows the raw 3-hourly data, while the bottom row displays the interpolated hourly data. Each column represents a specific UTC time (00 to 09). The color scale indicates the intensity of the variables, with TMP in °C and RH in %. The interpolation process fills the temporal gaps between the 3-hourly observations, resulting in a continuous hourly dataset that matches the temporal resolution of the Himawari-8/AHI AOD product. Notably, the smooth transitions in values over time are evident in the interpolated data, particularly in the previously empty time slots (01, 02, 04, 05, 07, and 08 UTC).</p>
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<p>Schematic diagram of the RF model used for gap-filling the Himawari-8/AHI 1-hourly AOD product. The model integrates LDAPS meteorological variables (TMP, U_WS, V_WS, BLH, LHFL, HCDC, RH, DSSF, PRES, DPT) and model-based AOD data (CAMS, MERRA-2) as input features. The output is the predicted AOD (AOD Pred), which is compared with the AHI AOD for quality assurance. Only AHI AOD data classified as “very good” quality were used for model training and validation. Solid lines represent the flow and processing of data, while dashed lines indicate comparisons with reference data.</p>
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<p>Schematic representation of the model validation process: The validation process includes a training set used for 5-fold CV to tune hyperparameters and a separate test set for final evaluation. The training set is divided into five subsets for CV, with each subset serving as a validation set once, while the remaining four subsets are used for training. The test set is kept entirely separate to independently evaluate the model’s performance in the final step.</p>
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<p>Density scatter plots comparing observed versus predicted AHI hourly AOD values using the gap-filling model with all features for (<b>a</b>) the 5-fold CV set (n = 433,286) and (<b>b</b>) the blind test set (n = 100,000). The color scale represents the number of data points per pixel, with warmer colors indicating higher densities. The 1:1 line (indicating perfect prediction) is in black, while the red line represents the fitted regression line. Statistical metrics (MBE, MAE, RMSE, CC) and the fitted line equations are provided in the top left corner of each plot.</p>
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<p>Difference in Correlation Coefficient (CC) between the entire test dataset and the subsets divided by value ranges. A higher CC is observed for (<b>a</b>) the entire test dataset, while lower CC values are observed for (<b>b</b>) the low-value group, (<b>c</b>) the medium-value group, and (<b>d</b>) the high-value group. Black circles represent the portions of data corresponding to each group, while gray ellipses show the entirety of the data.</p>
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<p>Geographical distribution of the six AERONET sites used for validation in South Korea. The sites include Gangneung_WNU (forest-adjacent), Seoul_SNU (urban), Hankuk_UFS (forest-adjacent), Anmyon (forest), and Gwangju_GIST (urban). The map illustrates the diverse environmental settings of these sites, covering urban areas, forests, and coastal regions across the Korean Peninsula.</p>
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<p>Scatter plots comparing (<b>a</b>) original Himawari-8 AOD data (only matched pairs between Himawari-8 and AERONET observations) and (<b>b</b>) gap-filled AOD data (only predicted values from the gap-filling model, excluding original Himawari-8 data) against AERONET AOD observations. Plot (<b>a</b>) was represented as a scatter plot because of smaller samples (n = 346), whereas plot (<b>b</b>) was illustrated as a density plot due to larger sample size (n = 4149) with blue tones indicating higher densities. The 1:1 line is shown in black. Statistical metrics, including MBE, MAE, RMSE, and CC, are also provided in the top left corner.</p>
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<p>Variable importance of the RF model for AOD gap-filling.</p>
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<p>Comparison of daily MERRA-2 and CAMS AOD values for 2019, with the dashed red line representing the 1:1 line.</p>
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<p>Monthly maps of gap-filled Himawari-8/AHI AOD for the year 2019, illustrating the spatial distribution of AOD values (ranging from 0 to 1) for each month. These maps provide a comprehensive view of the seasonal and regional variations in AOD throughout the year.</p>
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<p>Monthly maps of gap-filled Himawari-8/AHI AOD for the year 2019, illustrating the spatial distribution of AOD values (ranging from 0 to 1) for each month. These maps provide a comprehensive view of the seasonal and regional variations in AOD throughout the year.</p>
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<p>Time series comparison of Himawari-8 gap-filled AOD (purple) and AERONET AOD (brown) measurements during 16–22 March 2019, at different sites in South Korea: (<b>a</b>) Anmyon representing a forest environment, (<b>b</b>) Seoul_SNU representing an urban environment, and (<b>c</b>) Hankuk_UFS representing a near-forest environment. The middle panels (19 March) highlight the model’s performance during the high-concentration event, while the surrounding dates demonstrate its capability to capture typical conditions.</p>
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<p>Representative examples of hourly AOD gap-filling results for 2019, showing sample sets from each of the 12 months (January to December) to reflect different seasonal patterns. Each row displays 8 consecutive hours (00–07 UTC) of AOD data for a selected date. The “Before” rows show the original AOD data with gaps, while the “After” rows present the gap-filled data. A color scale indicates AOD values, ranging from 0 (blue) to 1 (red).</p>
Full article ">Figure A1 Cont.
<p>Representative examples of hourly AOD gap-filling results for 2019, showing sample sets from each of the 12 months (January to December) to reflect different seasonal patterns. Each row displays 8 consecutive hours (00–07 UTC) of AOD data for a selected date. The “Before” rows show the original AOD data with gaps, while the “After” rows present the gap-filled data. A color scale indicates AOD values, ranging from 0 (blue) to 1 (red).</p>
Full article ">Figure A1 Cont.
<p>Representative examples of hourly AOD gap-filling results for 2019, showing sample sets from each of the 12 months (January to December) to reflect different seasonal patterns. Each row displays 8 consecutive hours (00–07 UTC) of AOD data for a selected date. The “Before” rows show the original AOD data with gaps, while the “After” rows present the gap-filled data. A color scale indicates AOD values, ranging from 0 (blue) to 1 (red).</p>
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13 pages, 2883 KiB  
Article
The Effect of Accelerated Storage Temperature Conditions on the Shelf Life of Pasteurized Orange Juice Based on Microbiological, Physicochemical, and Color Attributes
by Theofilos Frangopoulos, Antonios Koliouskas and Dimitrios Petridis
Appl. Sci. 2024, 14(23), 10870; https://doi.org/10.3390/app142310870 - 24 Nov 2024
Viewed by 308
Abstract
The accelerated life testing (ASLT) method was used to evaluate the effect of increasing the storage temperature from 10 to 40 °C on the aerobic plate count (APC), the pH, and the colorimetric parameters (L*, a*, b*) of pasteurized orange juice during 40 [...] Read more.
The accelerated life testing (ASLT) method was used to evaluate the effect of increasing the storage temperature from 10 to 40 °C on the aerobic plate count (APC), the pH, and the colorimetric parameters (L*, a*, b*) of pasteurized orange juice during 40 days of storage. For APC growth, a polynomial model was found to fit better, and at the lower temperatures of 10 and 15 °C, the shelf life was longer, as expected. More specifically, 15 and 10 days were needed, respectively, until the rise in the APC population to 1000 cfu/mL. However, for the temperature range of 30–40 °C, only approximately 3 days were needed to reach 1000 cfu/mL APC. Regarding pH, according to an exponential 3P model, a stable trend was apparent at all temperatures until 30 days of storage, followed by a more abrupt decreasing trend at 25 °C. The lightness (L*), redness (a*), and yellowness (b*) of the juice showed a decreasing trend with the temperature increase, and this trend was more profound at higher temperature levels. The multiple regression analysis between the predictors L*, a*, b*, pH, storage temperature, and the APC response showed an increase in APC growth when the colorimetric parameters decreased and the temperature increased. Full article
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<p>Population changes of APC as a function of storage time in different temperature levels.</p>
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<p>Degradation profiler of APC versus period (days) and temperature level using a polynomial model.</p>
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<p>Curve fitting plot of experimental data in the exponential 3P model of pH vs. storage days. (<b>a</b>) 4 °C; (<b>b</b>) 15 °C; (<b>c</b>) 20 °C; (<b>d</b>) 25 °C.</p>
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<p>Curve fitting plot of experimental data in the logistic 3P model of L* value vs. storage days. (<b>a</b>) 4 °C; (<b>b</b>) 15 °C; (<b>c</b>) 20 °C; (<b>d</b>) 25 °C.</p>
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<p>Curve fitting plot of experimental data in the linear model of a* value vs. storage days. (<b>a</b>) 4 °C; (<b>b</b>) 15 °C; (<b>c</b>) 20 °C; (<b>d</b>) 25 °C.</p>
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<p>Curve fitting plot of experimental data in the linear model of b* value vs. storage days. (<b>a</b>) 4 °C; (<b>b</b>) 15 °C; (<b>c</b>) 20 °C; (<b>d</b>) 25 °C.</p>
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<p>Prediction profiler of APC (cfu/mL) baseline in relation to L*, a*, b*, pH, and temperature level (°C).</p>
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14 pages, 2934 KiB  
Article
Detecting Honey Adulteration: Advanced Approach Using UF-GC Coupled with Machine Learning
by Irene Punta-Sánchez, Tomasz Dymerski, José Luis P. Calle, Ana Ruiz-Rodríguez, Marta Ferreiro-González and Miguel Palma
Sensors 2024, 24(23), 7481; https://doi.org/10.3390/s24237481 - 23 Nov 2024
Viewed by 267
Abstract
This article introduces a novel approach to detecting honey adulteration by combining ultra-fast gas chromatography (UF-GC) with advanced machine learning techniques. Machine learning models, particularly support vector regression (SVR) and least absolute shrinkage and selection operator (LASSO), were applied to predict adulteration in [...] Read more.
This article introduces a novel approach to detecting honey adulteration by combining ultra-fast gas chromatography (UF-GC) with advanced machine learning techniques. Machine learning models, particularly support vector regression (SVR) and least absolute shrinkage and selection operator (LASSO), were applied to predict adulteration in orange blossom (OB) and sunflower (SF) honeys. The SVR model achieved R2 values above 0.90 for combined honey types. Treating OB and SF honeys separately resulted in a significant accuracy improvement, with R2 values exceeding 0.99. LASSO proved especially effective when honey types were treated individually. The integration of UF-GC with machine learning not only provides a reliable method for detecting honey adulteration, but also sets a precedent for future research in the application of this technique to other food products, potentially enhancing food authenticity across the industry. Full article
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<p>Circular dendrogram resulting from the hierarchical cluster analysis (HCA) of the dataset (D<sub>44<span class="html-italic">x</span>20002</sub>) with UF-GC; The names of the honey samples are colored according to their botanical origin: sunflower (purple) and orange blossom (orange). The four main clusters have been colored and labeled with letters A, B, C, and D. The average method with Euclidean distances was used.</p>
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<p>Score plot of PCA for orange blossom and sunflower honey.</p>
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20 pages, 1456 KiB  
Article
Development of All-Solid-State Potentiometric Sensors for Monitoring Carbendazim Residues in Oranges: A Degradation Kinetics Investigation
by Yasmeen A. A. Hassan, Sherif Okeil, Miriam F. Ayad, Lobna A. Hussein and Nermine V. Fares
Chemosensors 2024, 12(12), 246; https://doi.org/10.3390/chemosensors12120246 - 23 Nov 2024
Viewed by 130
Abstract
Monitoring fungicide residues in orange fruits is vital, as fungicides for orange cultivation are increasingly used to prevent yield loss. At the same time, increasing restrictions are added by regulatory organizations. For facile on-site monitoring of the fungicide carbendazim (MBC), five ion-selective potentiometric [...] Read more.
Monitoring fungicide residues in orange fruits is vital, as fungicides for orange cultivation are increasingly used to prevent yield loss. At the same time, increasing restrictions are added by regulatory organizations. For facile on-site monitoring of the fungicide carbendazim (MBC), five ion-selective potentiometric sensors are proposed and compared. The first two sensors were prepared with a precipitation-based technique using molybdate (sensor 1) and tetraphenylborate (TPB) (sensor 2), respectively. Furthermore, two ionophore-based sensors were prepared using β-cyclodextrin as ionophore together with TPB (sensor 3) and tetrakis(4-chlorophenyl)borate (TpClPB) (sensor 4) as ion-exchanger. Further incorporation of multi-walled carbon nanotubes (MWCNTs) between the graphite rod and the sensing membrane of sensor 4 (sensor 5) further improved the stability and significantly lowered the limit of detection (LOD). Their performance was evaluated according to IUPAC recommendations, revealing linear response in the concentration range 1 × 10−4–1 × 10−2 M, 1 × 10−5–1 × 10−2 M, 1 × 10−5–1 × 10−3 M, 1 × 10−6–1 × 10−3 M, and 1 × 10−7–1 × 10−3 M with a Nernstian slope of 54.56, 55.48, 56.00, 56.85, and 57.34 mV/decade, respectively. The LOD values for the five sensors were found to be 7.92 × 10−5, 9.98 × 10−6, 9.72 × 10−6, 9.61 × 10−7, and 9.57 × 10−8 M, respectively. The developed potentiometric sensors were successfully applied to determine the residue and degradation rate of MBC in orange samples. After the researched fungicide was applied to the orange trees, the preharvest interval (PHI) could be calculated based on the MBC degradation kinetics determined in the tested orange samples. Full article
22 pages, 11232 KiB  
Article
Climatological Trends and Effects of Aerosols and Clouds on Large Solar Parks: Application Examples in Benban (Egypt) and Al Dhafrah (UAE)
by Harshal Dhake, Panagiotis Kosmopoulos, Antonis Mantakas, Yashwant Kashyap, Hesham El-Askary and Omar Elbadawy
Remote Sens. 2024, 16(23), 4379; https://doi.org/10.3390/rs16234379 - 23 Nov 2024
Viewed by 215
Abstract
Solar energy production is vastly affected by climatological factors. This study examines the impact of two primary climatological factors, aerosols and clouds, on solar energy production at two of the world’s largest solar parks, Benban and Al Dhafrah Solar Parks, by using Earth [...] Read more.
Solar energy production is vastly affected by climatological factors. This study examines the impact of two primary climatological factors, aerosols and clouds, on solar energy production at two of the world’s largest solar parks, Benban and Al Dhafrah Solar Parks, by using Earth observation data. Cloud microphysics were obtained from EUMETSAT, and aerosol data were obtained from the CAMS and assimilated with MODIS data for higher accuracy. The impact of both factors was analysed by computing their trends over the past 20 years. These climatological trends indicated the variations in the change in each of the factors and their resulting impact over the years. The trends were quantified into the actualised drop in energy production (Wh/m2/year) in order to obtain the impact of each factor. Aerosols displayed a falling trend of −17.78 Wh/m2/year for Benban and −44.88 Wh/m2/year for Al Dhafrah. Similarly, clouds also portrayed a largely falling trend for both stations, −36.29 Wh/m2/year (Benban) and −70.27 Wh/m2/year (Al Dhafrah). The aerosol and cloud trends were also observed on a monthly basis to study their seasonal variation. The trends were further translated into net increases/decreases in the energy produced and the resulting emissions released. The analysis was extended to quantify the economic impact of the trends. Owing to the falling aerosol and cloud trends, the annual production was foreseen to increase by nearly 1 GWh/year (Benban) and 1.65 GWh/year (Al Dhafrah). These increases in annual production estimated reductions in emission released of 705.2 tonne/year (Benban) and 1153.7 tonne/year (Al Dhafrah). Following these estimations, the projected revenue was foreseen to increase by 62,000 USD/year (Benban) and 100,000 USD/year (Al Dhafrah). Considering the geographical location of both stations, aerosols evidently imparted a larger impact compared with clouds. Severe dust storm events were also analysed at both stations to examine the worst-case scenario of aerosol impact. The results show that the realized losses during these events amounted to 2.86 GWh for Benban and 5.91 GWh for Al Dhafrah. Thus, this study showcases the benefits of Earth observation technology and offers key insights into climatological trends for solar energy planning purposes. Full article
(This article belongs to the Section Urban Remote Sensing)
12 pages, 3497 KiB  
Article
Selenium Disulfide from Sustainable Resources: An Example of “Redneck” Chemistry with a Pinch of Salt
by Eduard Tiganescu, Shahrzad Safinazlou, Ahmad Yaman Abdin, Rainer Lilischkis, Karl-Herbert Schäfer, Claudia Fink-Straube, Muhammad Jawad Nasim and Claus Jacob
Materials 2024, 17(23), 5733; https://doi.org/10.3390/ma17235733 - 23 Nov 2024
Viewed by 361
Abstract
Selenium disulfide (often referred to as SeS2) encompasses a family of mixed selenium-sulfide eight-membered rings, traditionally used as an anti-dandruff agent in shampoos. SeS2 can be produced by reacting hydrogen sulfide (H2S) with selenite (SeO32−) [...] Read more.
Selenium disulfide (often referred to as SeS2) encompasses a family of mixed selenium-sulfide eight-membered rings, traditionally used as an anti-dandruff agent in shampoos. SeS2 can be produced by reacting hydrogen sulfide (H2S) with selenite (SeO32−) under acidic conditions. This chemistry is also possible with natural spring waters that are rich in H2S, thus providing an avenue for the more sustainable, green production of high-quality SeS2 particles from an abundant natural source. The orange material obtained this way consists of small globules with a diameter in the range of 1.1 to 1.2 µm composed of various SexS8−x chalcogen rings. It shows the usual composition and characteristics of a Se-S interchalcogen compound in EDX and Raman spectroscopy. Since the mineral water from Bad Nenndorf is also rich in salts, the leftover brine has been evaporated to yield a selenium-enriched salt mixture similar to table salt. As the water from Bad Nenndorf—in comparison to other bodies of water around the world—is still rather modest in terms of its H2S content, especially when compared with volcanic waters, this approach may be refined further to become economically and ecologically viable, especially as a regional business model for small and medium-sized enterprises. Full article
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<p>The H<sub>2</sub>S concentration present in the water samples gradually decreases as affirmed using the MB assay.</p>
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<p>The water was collected from the underground spring in a field near Bad Nenndorf in northern Germany. A preliminary reaction was carried out “redneck style” at the source of origin, and an immediate change in color confirmed the feasibility of the synthesis. The figure also represents the chemistry carried out in the laboratory and shows a photograph of the orange material obtained.</p>
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<p>Raman spectroscopy confirmed the presence of S-S, S-Se, and Se-Se bonds.</p>
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<p>SeS<sub>2</sub> was analyzed to determine chemical composition using EDX coupled to SEM. EDX confirmed the presence of selenium and sulfur at a ratio of around 1:2 (Panel (<b>a</b>)), while the SEM image showed the presence of (aggregated) globular material (Panel (<b>b</b>)).</p>
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<p>The filtrate was evaporated at 50 °C to obtain salt (Panel (<b>a</b>)), which was analyzed by SEM (Panel (<b>b</b>)) coupled with EDX (Panel (<b>c</b>)) to quantify the elements present in the salt. EDX confirmed the presence of selenium at about 0.40% <span class="html-italic">w</span>/<span class="html-italic">w</span> (dry weight), as compared to the overall salt composition.</p>
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<p>H<sub>2</sub>S springs may serve as sources for the production of value-added products, such as SeS<sub>2,</sub> and avoid wasting this natural resource as sewage. This strategy not only opens up the door for boosting local economies but also, as a true “hat trick”, decreases the environmental burden posed by the chemical treatment of H<sub>2</sub>S-rich water.</p>
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18 pages, 468 KiB  
Article
Functional Properties of Rapeseed Honey Enriched with Lyophilized Fruits
by Aleksandar Marić, Marijana Sakač, Pavle Jovanov, Branislava Đermanović, Nemanja Teslić, Dragana Plavšić and Dimitar Jakimov
Agriculture 2024, 14(12), 2117; https://doi.org/10.3390/agriculture14122117 - 22 Nov 2024
Viewed by 296
Abstract
This study evaluates the physicochemical characteristics, antioxidant, antibacterial, and antiproliferative properties of rapeseed honey collected from Vojvodina, Serbia, as well as rapeseed honey-based products enriched with 10% fruit lyophilizate, including sour cherry (Prunus cerasus), strawberry (Fragaria), blueberry (Vaccinium [...] Read more.
This study evaluates the physicochemical characteristics, antioxidant, antibacterial, and antiproliferative properties of rapeseed honey collected from Vojvodina, Serbia, as well as rapeseed honey-based products enriched with 10% fruit lyophilizate, including sour cherry (Prunus cerasus), strawberry (Fragaria), blueberry (Vaccinium myrtillus), raspberry (Rubus idaeus), blackberry (Rubus fruticosus), orange (Citrus sinensis), and pineapple (Ananas comosus). Honey-based products with lyophilizates were developed to enhance the relatively limited therapeutic potential of rapeseed honey by incorporating fruit lyophilizates known to possess bioactive compounds. The moisture content, pH, electrical conductivity, free acidity, hydroxymethylfurfural (HMF), and mineral composition were analyzed. Sour cherry-enriched honey exhibited the highest total phenolic content (TPC = 102 ± 0.18 mg GAE/100 g), while blueberry-enriched honey had the highest total flavonoid content (TFC = 34.9 ± 0.89 mg CAE/100 g) and total anthocyanin content (TAC = 299 ± 3.14 mg EC/100 g), with the greatest relative scavenging capacity (81.0 ± 0.46% of DPPH inhibition). Polyphenol profiling identified phenolic acids and flavonoids, with raspberry-enriched honey showing the highest total polyphenol content (47.0 ± 0.98 mg/kg) due to its high ellagic acid content (38.4 ± 1.11 mg/kg). All honey-based products demonstrated moderate antibacterial activity against Staphylococcus aureus and Staphylococcus epidermidis. Significant antiproliferative effects against breast (MCF-7), cervix (HeLa), and colon (HT-29) cancer cell lines were observed, particularly in pineapple and blueberry-enriched honey, with IC50 values as 9.04 ± 0.16 mg/mL and 9.95 ± 0.24 mg/mL for MCF-7 cells, respectively. Based on all the obtained results, it can be concluded that the enrichment of rapeseed honey with fruit lyophilizates at a 10% level contributed to an increase in the antioxidant, antibacterial, and antiproliferative properties of rapeseed honey. Full article
(This article belongs to the Section Agricultural Product Quality and Safety)
9 pages, 703 KiB  
Article
Production of Pectic Oligosaccharides from Citrus Peel via Steam Explosion
by Toni-Ann Martorano, Kyle L. Ferguson, Randall G. Cameron, Wei Zhao, Arland T. Hotchkiss, Hoa K. Chau and Christina Dorado
Foods 2024, 13(23), 3738; https://doi.org/10.3390/foods13233738 - 22 Nov 2024
Viewed by 458
Abstract
Steam explosion (STEX) of peel from commercially juice-extracted oranges was used to convert peel pectin into pectic oligosaccharides (POSs). Surprisingly uniform populations, based on the polydispersity index (PDI; weight-average molecular weight (Mw)/number-average molecular weight (Mn)) of POSs, were obtained [...] Read more.
Steam explosion (STEX) of peel from commercially juice-extracted oranges was used to convert peel pectin into pectic oligosaccharides (POSs). Surprisingly uniform populations, based on the polydispersity index (PDI; weight-average molecular weight (Mw)/number-average molecular weight (Mn)) of POSs, were obtained from the Hamlin and Valencia varieties of Citrus sinensis. The POSs from Hamlin and Valencia peel had PDI values of (1.23 ± 0.01, 1.24 ± 0.1), respectively. The Mw values for these samples were 14.9 ± 0.2 kDa for Hamlin, and 14.5 ± 0.1 kDa for Valencia, respectively. The degree of methyl-esterification (DM) was 69.64 ± 3.18 for Hamlin and 65.51 ± 1.61 for Valencia. The composition of the recovered POSs was dominated by galacturonic acid, ranging from 89.1% to 99.6% of the major pectic sugars. Only the Hamlin sample had a meaningful amount of rhamnose present, indicating the presence of an RG I domain. Even so, the Hamlin sample’s degree of branching (DBr) was very low (2.95). Full article
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Figure 1
<p>High-performance size-exclusion chromatography analysis of the Hamlin (DM-D4) (<b>A</b>) and Valencia (DM-D6) (<b>B</b>) varieties; superimposed calibration curve of Hamlin and Valencia (DM-D4, DM-D6) (<b>C</b>). In <a href="#foods-13-03738-f001" class="html-fig">Figure 1</a>A,B, HPSEC detectors were light scattering at 90 °C (<span style="color:red">-</span>), differential pressure viscometer (-), refractive index (<span style="color:#0070C0">-</span>), and ultraviolet absorption at 280 nm (<span style="color:#92D050">-</span>). In <a href="#foods-13-03738-f001" class="html-fig">Figure 1</a>C, HPSEC detectors were light scattering at 90 °C, (DM-D4) and HPSEC detectors were light scattering at 90 °C, DM-D6.</p>
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56 pages, 27840 KiB  
Article
Citrus: From Symbolism to Sensuality—Exploring Luxury and Extravagance in Western Muslim Bustān and European Renaissance Gardens
by Diego Rivera, Julio Navarro, Inmaculada Camarero, Javier Valera, Diego-José Rivera-Obón and Concepción Obón
Arts 2024, 13(6), 176; https://doi.org/10.3390/arts13060176 - 21 Nov 2024
Viewed by 291
Abstract
This study delves into the multifaceted realm of citrus fruits, exploring their significance and socioeconomic implications from their early introduction to Western Muslim and Renaissance gardens, tracing their journey throughout history. Employing a multidisciplinary approach, drawing from biological, archaeobotanical, iconographic, and textual sources, [...] Read more.
This study delves into the multifaceted realm of citrus fruits, exploring their significance and socioeconomic implications from their early introduction to Western Muslim and Renaissance gardens, tracing their journey throughout history. Employing a multidisciplinary approach, drawing from biological, archaeobotanical, iconographic, and textual sources, our study offers a comprehensive exploration of citrus symbolism and cultural significance, integrating historical, artistic, horticultural, and socioeconomic viewpoints. The genus Citrus (Rutaceae) comprises around thirty species and its natural habitat spans from the southern slopes of the Himalayas to China, Southeast Asia, nearby islands, and Queensland. Originating from only four of these species, humans have cultivated hundreds of hybrids and thousands of varieties, harnessing their culinary, medicinal, and ornamental potential worldwide. We delve into the symbolic value of citrus fruits, which have served as indicators of economic status and power. From their early presence in Mediterranean religious rituals to their depiction in opulent Roman art and mythical narratives like the Garden of the Hesperides, citrus fruits have epitomized luxury and desire. Christian lore intertwines them with the forbidden fruit of Eden, while Islamic and Sicilian gardens and Renaissance villas signify their prestige. We analyze diverse perspectives, from moralists to hedonists, and examine their role in shaping global agriculture, exemplified by rare varieties like aurantii foetiferi. Full article
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<p>Citrus diversity: A. pummelo (<span class="html-italic">Citrus maxima</span>); B. lemon of Amalfi (<span class="html-italic">C.</span> × <span class="html-italic">limon</span> var. <span class="html-italic">limon</span>); C. lemon “Feminello” (<span class="html-italic">C.</span> × <span class="html-italic">limon</span> var. <span class="html-italic">limon</span>); D. navel orange (<span class="html-italic">C.</span> × <span class="html-italic">aurantium</span> var. <span class="html-italic">sinensis</span>); E. sour orange (<span class="html-italic">C.</span> × <span class="html-italic">aurantium</span> var. <span class="html-italic">aurantium</span>); F. limetta (<span class="html-italic">C.</span> × <span class="html-italic">limon</span> var. <span class="html-italic">limetta</span>); G. grapefruit (<span class="html-italic">C.</span> × <span class="html-italic">aurantium</span> var. <span class="html-italic">paradisii</span>); H*. clementine (<span class="html-italic">C.</span> × <span class="html-italic">aurantium</span> var. <span class="html-italic">clementina</span>). I. Peretta lemon (<span class="html-italic">C.</span> × <span class="html-italic">limon</span> var. <span class="html-italic">limon</span>). J. Lime (<span class="html-italic">C.</span> × <span class="html-italic">aurantiifolia</span>). K. mellarosa (<span class="html-italic">C.</span> × <span class="html-italic">mellarosa</span>). L. bergamot (<span class="html-italic">C.</span> × <span class="html-italic">bergamia</span>); M*. sour mandarin (<span class="html-italic">C. reticulata</span>); N*. mandarin “Tardivo de Ciaculli” (<span class="html-italic">C.</span> × <span class="html-italic">aurantium</span> var. <span class="html-italic">deliciosa</span>). Image by Diego Rivera. Note: (*) taxa unlikely to have been present in the Mediterranean before 19th century CE.</p>
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<p>Etrog citrons on coins of the Bar Kokhba revolt in Israel: (<b>A</b>) Shekel of Bar Kokhba silver Tetradrachm, undated, attributed to year 3 (134/135 C.E.); (<b>B</b>) Copper coin of Israel revolt, 69–70 CE (year 4), etrog (citron) flanked by lulav (bound palm branch, myrtle, and willow) on either side; (<b>C</b>) Bar Kochba silver shekel, year 1 of the Freedom of Israel. In both, lulav thrice bound; in left field, etrog; (<b>D</b>) Copper coin of Israel revolt, 69–70 CE (year 4), lulav (bound palm branch, myrtle, and willow) flanked by an etrog (citron) on either side; (<b>E</b>) Common etrog citron in Florence Botanical Garden. Images: (<b>A</b>) by <a href="https://coinreplicas.com/product/shekel-of-bar-kokhba-silver-tetradrachm/" target="_blank">https://coinreplicas.com/product/shekel-of-bar-kokhba-silver-tetradrachm/</a>, accessed on 13 November 2024; (<b>B</b>) by <a href="https://www.britishmuseum.org/collection/object/C_1908-0110-12" target="_blank">https://www.britishmuseum.org/collection/object/C_1908-0110-12</a>, accessed on 13 November 2024; (<b>C</b>) by <a href="https://coinreplicas.com/product/shekel-of-bar-kochba-silver-tetradrachm-year-1/" target="_blank">https://coinreplicas.com/product/shekel-of-bar-kochba-silver-tetradrachm-year-1/</a>, accessed on 13 November 2024; (<b>D</b>) by <a href="https://www.britishmuseum.org/collection/object/C_G-2647" target="_blank">https://www.britishmuseum.org/collection/object/C_G-2647</a>, accessed on 13 November 2024; (<b>E</b>) by Diego Rivera and Concepción Obón.</p>
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<p>Elements of the Sukkot festival: (<b>A</b>) installation from October 2017 recreating a sukkah (ceremonial booth) adorned with flowers and fruits, constructed for the Sukkot festival. The interior displays a table with traditional ceremonial elements, particularly the lulav (palm frond bundle) and etrog citron; (<b>B</b>) nineteenth-century Italian silver etrog case; (<b>C</b>) Table arrangement featuring lulav bundles and kosher wine; (<b>D</b>) twentieth-century Moroccan wooden etrog case. All artifacts photographed at the Sephardic Museum in the ‘Synagogue of El Tránsito’, Toledo, Spain. Images: (<b>A</b>–<b>D</b>) by Diego Rivera.</p>
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<p>Lemons in Casa del Frutetto (Pompei Italy) 1st cent CE. (<b>A</b>) Depiction of a fruit garden adorning the walls within the hall; (<b>B</b>) Specific focus on the lemon tree’s fruiting (<span class="html-italic">Citrus</span> × <span class="html-italic">limon</span> var. <span class="html-italic">limon</span>), demarcated by a white rectangle in image (<b>A</b>). Images by Diego Rivera and Concepción Obón.</p>
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<p>Wall paintings at the Villa Livia, Prima Porta (1st century BCE). (<b>A</b>) Flat lemon (<span class="html-italic">Citrus</span> × <span class="html-italic">limon</span> var. <span class="html-italic">limon</span>) with quince-like flattened fruits [according to <a href="#B11-arts-13-00176" class="html-bibr">Andrews</a> (<a href="#B11-arts-13-00176" class="html-bibr">1961</a>), it is a citron tree]. (<b>B</b>) Flat lemon like the above, collected in 2016 in gardens close to the Avernus Lake, 4 km west of Pozzuoli (Campania, Italy). Images by Concepción Obón and Diego Rivera.</p>
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<p>Roman Villa del Casale (Piazza Armerina, Sicily, Italy) mosaics (285–305 CE). (<b>A</b>) Mosaic with citron tree branch with citrons; (<b>B</b>,<b>C</b>) Citron fruits (<span class="html-italic">Citrus medica</span>); (<b>D</b>) Mosaic with citron tree branches and citrons. Wild mandarins, pomegranate, and fig tree branches. Images: (<b>A</b>,<b>C</b>,<b>D</b>) by Diego Rivera and Concepción Obón; (<b>B</b>) by <a href="#B121-arts-13-00176" class="html-bibr">Risso and Poiteau</a> (<a href="#B121-arts-13-00176" class="html-bibr">1818–1822</a>).</p>
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<p>Lemon in terracotta (<span class="html-italic">Citrus</span> × <span class="html-italic">limon</span> var. <span class="html-italic">limon</span>), Roman ensemble Casón-Pedregal (Jumilla, Murcia, Spain), Museo Arqueológico de Jumilla. Image by Concepción Obón.</p>
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<p>(<b>A</b>) Mosaic from Tusculum, early 2nd cent CE, preserved in the National Roman Museum, with figures of lemons, citrons, oranges, and limettas. (<b>B</b>) Mosaic with garlands of fruits, among which we find notably citrons, pointed lemons, and chinottos or other reddish-orange fruit, 2nd to 3rd centuries CE, found in the 1959 excavations in the Plaza de la Corredera (Cordoba, Spain). Images: (<b>A</b>) by <a href="#B144-arts-13-00176" class="html-bibr">Tolkowsky</a> (<a href="#B144-arts-13-00176" class="html-bibr">1938</a>) and (<b>B</b>) by Diego Rivera.</p>
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<p>(<b>A</b>) The Moorish courtyard of the orange trees, with plenty of orange, lemon and other citrus trees, in the Alcázar de los Reyes Cristianos of Cordoba. (<b>B</b>) Patio de los Naranjos of the Mosque Cathedral of Cordoba (Spain). Images by Diego Rivera.</p>
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<p>Plants of the gardens illustrated in the <span class="html-italic">Ḥadīth Bayāḍ wa Riyāḍ</span>, 12th–13th cent CE, manuscript, Vatican Library 368: (<b>A</b>) (4 v.) Cypress, myrtle-like citrus tree and lawn; (<b>B</b>) (9 r.) Myrtle-like citrus tree and lawn; (<b>C</b>) (10 r.) Myrtle-like citrus tree and lawn; (<b>D</b>) (13 r.) Heavily pruned palm tree, lawn, bearded iris, and Arabian jasmine in a pergola; (<b>E</b>) (17 r.) Cypress, myrtle-like citrus tree and lawn; (<b>F</b>) (19 r.) Cypresses and lawn; (<b>G</b>) (26 v.) Myrtle-like citrus tree and lawn. Images by <a href="#B149-arts-13-00176" class="html-bibr">Vaticana</a> (<a href="#B149-arts-13-00176" class="html-bibr">2024</a>).</p>
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<p>The plants identified in the illustrations of the <span class="html-italic">Ḥadīth Bayāḍ wa Riyāḍ</span>: (<b>A</b>) <span class="html-italic">Cupressus sempervirens</span>, Kolymbetra Gardens, Valley of the Temples, Agrigento (Sicily, Italy); (<b>B</b>) <span class="html-italic">Phoenix dactylifera</span> Park of Elche (Spain); (<b>C</b>) <span class="html-italic">Citrus</span> × <span class="html-italic">aurantium</span> L. var. <span class="html-italic">myrtifolia</span>; (<b>D</b>) <span class="html-italic">Iris germanica</span>, Castell del Monte (Puglia, Italy); (<b>E</b>) <span class="html-italic">Myrtus communis</span> subsp. <span class="html-italic">baetica</span>, Molina de Segura (Murcia); (<b>F</b>) <span class="html-italic">Jasminum sambac</span>, Molina de Segura (Murcia). Images (<b>A</b>,<b>B</b>,<b>D</b>–<b>G</b>) by Diego Rivera, (<b>C</b>) by <a href="#B121-arts-13-00176" class="html-bibr">Risso and Poiteau</a> (<a href="#B121-arts-13-00176" class="html-bibr">1818–1822</a>).</p>
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<p>Plants illustrated in the <span class="html-italic">Sala de los Reyes</span> in the Alhambra, Vault of the Fountain of the Youth (Granada, Spain), 14th–15th cent CE: (<b>A</b>–<b>E</b>) orange tree (<span class="html-italic">Citrus</span> × <span class="html-italic">aurantium</span>); (<b>F</b>,<b>G</b>,<b>K</b>) Pinyon pine (<span class="html-italic">Pinus pinea</span>); (<b>H</b>,<b>L</b>) oleander (<span class="html-italic">Nerium oleander</span>) or Moorish myrtle (<span class="html-italic">Myrtus communis</span> subsp. <span class="html-italic">baetica</span>); (<b>I</b>) cf. climber rose or <span class="html-italic">Calystegia sepium</span>; (<b>J</b>,<b>M</b>) spreading cherry plum (<span class="html-italic">Prunus cocomilia</span>). Images from <a href="#B133-arts-13-00176" class="html-bibr">Simón</a> (<a href="#B133-arts-13-00176" class="html-bibr">2020</a>).</p>
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<p>Plants illustrated in the Sala de los Reyes, Vault of the Lady Playing Chess in the Alhambra (Granada, Spain), 14th–15th cent CE: (<b>A</b>–<b>C</b>) orange tree (<span class="html-italic">Citrus</span> × <span class="html-italic">aurantium</span>); (<b>D</b>,<b>J</b>) (lower half), branched tulip (cf. <span class="html-italic">Tulipa turkestanica</span>); (<b>E</b>). oleander (<span class="html-italic">Nerium oleander</span>) or Moorish myrtle (<span class="html-italic">Myrtus communis</span> subsp. <span class="html-italic">baetica</span>); (<b>F</b>) blooming tree with heart-shaped leaves, probably a mulberry with a climber rose; (<b>G</b>–<b>I</b>) oak tree (cf. <span class="html-italic">Quercus pyrenaica</span>); (<b>J</b>) isolated shrub, Rosa × <span class="html-italic">alba</span>; (<b>K</b>) Pinyon pine (<span class="html-italic">Pinus pinea</span>); (<b>L</b>,<b>M</b>) red tulip (cf. <span class="html-italic">Tulipa sprengeri</span>). Images from <a href="#B133-arts-13-00176" class="html-bibr">Simón</a> (<a href="#B133-arts-13-00176" class="html-bibr">2020</a>).</p>
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<p>Images of citrus trees in the manuscript “Códice Rico” of the Cantigas de Santa María, written and illustrated at the court of King Alfonso X the Wise between 1270 and 1282. (<b>A</b>) cf. orange tree near a fig tree, Fol. 28 V; (<b>B</b>) orange tree in the center of the Garden of Eden, with the serpent offering an orange to Eve, Fol. 88 V; (<b>C</b>) doubtful orange tree, alternatively a pine tree, Fol. 150 R; (<b>D</b>) garden of a cloister, with date palms and orange trees, Fol. 174 R. Images from <a href="#B106-arts-13-00176" class="html-bibr">Patrimonio</a> (<a href="#B106-arts-13-00176" class="html-bibr">2024</a>).</p>
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<p>The “Grand Bourbon” orange tree: (<b>A</b>) fruit and flowers of the “Grand Bourbon” orange tree in 1819 at the Versailles orangery, with an age of c. 400 years; (<b>B</b>) the “Grand Bourbon” orange tree in 1857 at the Versailles orangery, with an age of c. 430 years. Images: (<b>A</b>) by <a href="#B121-arts-13-00176" class="html-bibr">Risso and Poiteau</a> (<a href="#B121-arts-13-00176" class="html-bibr">1818–1822</a>), (<b>B</b>) by Freeman in <a href="#B32-arts-13-00176" class="html-bibr">Charton</a> (<a href="#B32-arts-13-00176" class="html-bibr">1857</a>).</p>
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<p>Plants of the Norman Gardens (Sicily, Italy), 12th–13th cent CE. Genoardo’s park, Palermo: (<b>A</b>) two stems of sugarcane (<span class="html-italic">Saccharum officinarum</span>); (<b>B</b>) grapevine (<span class="html-italic">Vitis vinifera</span>); (<b>C</b>) oleander (<span class="html-italic">Nerium oleander</span>) bush; (<b>D</b>) citrus tree without fruits; (<b>E</b>) fruiting date palm (<span class="html-italic">Phoenix dactylifera</span>); (<b>F</b>) cypress with an oval lanceolate crown, some of its branches spreading beyond the regular limit of the crown (<span class="html-italic">Cupressus sempervirens</span>). Image from <a href="#B50-arts-13-00176" class="html-bibr">Delle Donne</a> (<a href="#B50-arts-13-00176" class="html-bibr">2024</a>).</p>
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<p>Images of plants in the Norman Palaces (Sicily, Italy), 12th–13th cent CE. Zisa palace, Palermo: Citrus tree flanked by two date palms (<span class="html-italic">Phoenix dactylifera</span>). Image: <a href="https://commons.wikimedia.org/wiki/File:Mosa&#xEF;que_de_la_Zisa_(Palerme)_(7035275791).jpg" target="_blank">https://commons.wikimedia.org/wiki/File:Mosaïque_de_la_Zisa_(Palerme)_(7035275791).jpg</a>, accessed on 13 November 2024.</p>
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<p>Images of Plants in the Norman Palaces (Sicily, Italy), 12th–13th cent CE. Chambers of Roger II, Royal palace, Palermo: (<b>A</b>) above, citrus trees and date palm trees. Below, date palm tree (<span class="html-italic">Phoenix dactylifera</span>) flanked by two lions and two citrus trees; (<b>B</b>) detail of date palm and olive tree; (<b>C</b>) old, tall date palm; (<b>D</b>) above, date palm flanked by centaurs and citrus trees. Below, fig tree flanked by leopards, date palms, citrus trees, and peacocks; (<b>E</b>) above, citrus trees and date palm trees. Below, olive trees and date palms. Images: (<b>A</b>,<b>C</b>,<b>E</b>) <a href="https://islamicart.museumwnf.org/database_item.php?id=monument;ISL;it;Mon01;17;it" target="_blank">https://islamicart.museumwnf.org/database_item.php?id=monument;ISL;it;Mon01;17;it</a>; (<b>B</b>) <a href="https://www.bbpalermo.it/wp-content/uploads/2016/12/Joharia-Sala-di-Ruggero-parete-occidentale-1.jpg" target="_blank">https://www.bbpalermo.it/wp-content/uploads/2016/12/Joharia-Sala-di-Ruggero-parete-occidentale-1.jpg</a>, accessed on 13 November 2024; (<b>D</b>) <a href="https://www.esplora.co.uk/wp-content/uploads/2023/04/IMG_9701-scaled.jpeg" target="_blank">https://www.esplora.co.uk/wp-content/uploads/2023/04/IMG_9701-scaled.jpeg</a>, accessed on 13 November 2024.</p>
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<p>Fruits of singular citrus varieties from historical Italian Renaissance collections: (<b>A</b>) lumia, Oscar Tintori; (<b>B</b>) limone incanellato, Boboli; (<b>C</b>) limone della Procida, Boboli; (<b>D</b>) limone cedrato, Giardino Botanico Firenze; (<b>E</b>) limone—medica Fiorentina, Giardino Botanico Firenze; (<b>F</b>) cedro di Roma, Oscar Tintori; (<b>G</b>) limetta, Oscar Tintori; (<b>H</b>). peretta di S. Domenico, Oscar Tintori; (<b>I</b>). bergamotto feminello, Giardino Botanico Firenze; (<b>J</b>) limoncello di Spagna, Oscar Tintori; (<b>K</b>) Arancia cornicolata, Giardino Botanico Firenze; (<b>L</b>). Arancia canalicolata, Oscar Tintori. Images by Concepción Obón and Diego Rivera.</p>
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<p>Citrus in the late 15th century CE “mille fleurs” tapestries of the Cluny Museum (Paris): (<b>A</b>) set of the “Four Tapestries”, orange tree with flowers and fruits. In the next set of “The Lady and the Unicorn”, the scenes are placed in the framework of a garden with four trees and hundreds of herbaceous flowers; (<b>B</b>) orange tree with blossoms and fruits in the Desire tapestry; (<b>C</b>) blossoming orange trees with fruits in the “sense of Touch” tapestry; (<b>D</b>) similar trees in the “sense of Hearing” tapestry; (<b>E</b>) similar trees in the “sense of Smell” tapestry; (<b>F</b>) one single tall orange tree in the “sense of Taste” tapestry. Images by Diego Rivera.</p>
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<p>Hybrids of citron: (<b>A</b>) pompia (hybrid of <span class="html-italic">C. medica</span> × <span class="html-italic">C.</span> × <span class="html-italic">aurantium</span>) from the Farnesina Palace (Rome, Italy), also known as <span class="html-italic">C. medica</span> “Aurantiata” or “cedro della Cina”; (<b>B</b>) lumia pyriforme (hybrid of <span class="html-italic">C. medica</span> × <span class="html-italic">C. maxima</span>), Oscar Tintori greenhouses. Images by Diego Rivera and Concepción Obón.</p>
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<p>(<b>A</b>) Orange tree growing in a large terracotta pot in the garden of the Farnesina Palace (Rome, Italy). (<b>B</b>) Buddha’s hand tree in a large terracotta pot in the garden of the Villa Borghese (Rome, Italy). This form of cultivation was widely used in Renaissance villas to facilitate the protection of citrus plants during the frost season in places such as Florence, Rome, Nuremberg, El Escorial, or Fontainebleau. (<b>C</b>) Citrus winter conservatory, orangery, or “limonaia” at Boboli (Florence, Italy). Images (<b>A</b>,<b>B</b>) by Concepción Obón, (<b>C</b>) by Diego Rivera.</p>
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<p>Bartolomeo Bimbi, four canvases of citrus: (<b>A</b>) Arance, bergamotti, cedri, limoni e lumie, 1715; (<b>B</b>) Arance, lime, limoni e lumie, 1715; (<b>C</b>) Melangoli, cedri e limoni, 1715; (<b>D</b>) Arance, cedri, lime, limoni e lumie, 1715. Images by Wikimedia Commons. (<b>A</b>) <a href="https://commons.wikimedia.org/wiki/File:Bartolomeo_bimbi,_arance,_bergamotti,_cedri,_limoni_e_lumie,_1715,_01.JPG" target="_blank">https://commons.wikimedia.org/wiki/File:Bartolomeo_bimbi,_arance,_bergamotti,_cedri,_limoni_e_lumie,_1715,_01.JPG</a>, accessed on 12 November 2024; (<b>B</b>) <a href="https://commons.wikimedia.org/wiki/File:Bartolomeo_bimbi,_arance,_lime,_limoni_e_lumie,_1715.JPG" target="_blank">https://commons.wikimedia.org/wiki/File:Bartolomeo_bimbi,_arance,_lime,_limoni_e_lumie,_1715.JPG</a>, accessed on 12 November 2024; (<b>C</b>) <a href="https://commons.wikimedia.org/wiki/File:Bartolomeo_bimbi,_melagoli,_cedri_e_limoni,_1715,_01.JPG" target="_blank">https://commons.wikimedia.org/wiki/File:Bartolomeo_bimbi,_melagoli,_cedri_e_limoni,_1715,_01.JPG</a>, accessed on 12 November 2024; (<b>D</b>) <a href="https://commons.wikimedia.org/wiki/File:Bartolomeo_bimbi,_arance,_cedri,_lime,_limoni_e_lumie,_1715,_01.JPG" target="_blank">https://commons.wikimedia.org/wiki/File:Bartolomeo_bimbi,_arance,_cedri,_lime,_limoni_e_lumie,_1715,_01.JPG</a>, accessed on 12 November 2024.</p>
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<p>Fruit and flowers of orange tree, together with roses or peonies, in the decoration of the Loggia di Psyche in the Villa Farnesina (1517-18), from the garlands surrounding the scene “Psyche Brings a Vessel up to Venus” by Raffaello and his workshop (Rome). Image by Concepción Obón.</p>
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<p>Citrus pollen and seed surface patterns, SEM images: (<b>A</b>) <span class="html-italic">C. medica</span> var. <span class="html-italic">sarcodactylis</span> anther with pollen grains and (<b>B</b>) <span class="html-italic">C. medica</span> var. <span class="html-italic">sarcodactylis</span> pollen grains; (<b>C</b>) <span class="html-italic">C.</span> × <span class="html-italic">aurantiifolia</span> seed surface; (<b>D</b>) <span class="html-italic">C.</span> × <span class="html-italic">limon</span> var. <span class="html-italic">limetta</span> seed surface; (<b>E</b>) <span class="html-italic">C.</span> × <span class="html-italic">limon</span> var. <span class="html-italic">limon</span> seed surface; (<b>F</b>) <span class="html-italic">C. medica</span> “etrog” seed surface; (<b>G</b>) <span class="html-italic">C.</span> × <span class="html-italic">aurantium</span> var. <span class="html-italic">aurantium</span> seed surface; (<b>H</b>) <span class="html-italic">C.</span> × <span class="html-italic">aurantium</span> var. <span class="html-italic">paradisii</span> seed surface. Images by Teresa Coronado Parra, Service of Microscopy, Murcia University.</p>
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20 pages, 8072 KiB  
Article
Using a Webcam to Assess Upper Extremity Proprioception: Experimental Validation and Application to Persons Post Stroke
by Guillem Cornella-Barba, Andria J. Farrens, Christopher A. Johnson, Luis Garcia-Fernandez, Vicky Chan and David J. Reinkensmeyer
Sensors 2024, 24(23), 7434; https://doi.org/10.3390/s24237434 - 21 Nov 2024
Viewed by 338
Abstract
Many medical conditions impair proprioception but there are few easy-to-deploy technologies for assessing proprioceptive deficits. Here, we developed a method—called “OpenPoint”—to quantify upper extremity (UE) proprioception using only a webcam as the sensor. OpenPoint automates a classic neurological test: the ability of a [...] Read more.
Many medical conditions impair proprioception but there are few easy-to-deploy technologies for assessing proprioceptive deficits. Here, we developed a method—called “OpenPoint”—to quantify upper extremity (UE) proprioception using only a webcam as the sensor. OpenPoint automates a classic neurological test: the ability of a person to use one hand to point to a finger on their other hand with vision obscured. Proprioception ability is quantified with pointing error in the frontal plane measured by a deep-learning-based, computer vision library (MediaPipe). In a first experiment with 40 unimpaired adults, pointing error significantly increased when we replaced the target hand with a fake hand, verifying that this task depends on the availability of proprioceptive information from the target hand, and that we can reliably detect this dependence with computer vision. In a second experiment, we quantified UE proprioceptive ability in 16 post-stroke participants. Individuals post stroke exhibited increased pointing error (p < 0.001) that was correlated with finger proprioceptive error measured with an independent, robotic assessment (r = 0.62, p = 0.02). These results validate a novel method to assess UE proprioception ability using affordable computer technology, which provides a potential means to democratize quantitative proprioception testing in clinical and telemedicine environments. Full article
(This article belongs to the Special Issue Advanced Sensors in Biomechanics and Rehabilitation)
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Figure 1
<p>The visual display of the OpenPoint proprioception assessment, as implemented with a webcam. (<b>A</b>): The start position for the pointing task. Note that the image displayed to the participant is mirrored, so the user’s left hand appears on the left side of the screen. The assessment requires users to touch the fingertip of one hand with the fingertip of the other hand. The hand on the torso is the “target hand”, which is normally obscured using a graphically overlaid polygon, as shown on the left. (<b>B</b>): We removed the polygon to illustrate the accuracy of the finger tracking algorithm. The user is instructed to raise their pointing finger to a start target indicated by the green circle. The software then shows a target on the tip of one of the fingers of the cartoon hand (red circle). Following a three second countdown, the user is given an instruction to point and tries to touch the fingertip on their target hand, which is hidden by the polygon. Participants were instructed to refrain from directly looking at their own target hand. The tracking algorithm robustly tracks both fingertips and determines when the pointing finger stops moving, measuring the pointing error to assess proprioceptive ability.</p>
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<p>Pointing error calculation. (<b>A</b>) Example output from MediaPipe. The orange lines connect the landmarks returned by MediaPipe when the fingers are fully extended. We defined pointing error as the distance between the fingertips in the frontal plane (blue line). (<b>B</b>) Results from a simple experiment where the participants kept the distance between their fingers constant but moved their hands away from the camera by sliding backward on a rolling chair. The pixel-based pointing error (blue) decreased as the individual rolled back from the camera, as did apparent hand size, measured in pixels (orange line). The pixel-based pointing error (blue) has been multiplied by six to better show the decrease in distance. Dividing pixel-based pointing error by <math display="inline"><semantics> <mrow> <mi>h</mi> <mi>a</mi> <mi>n</mi> <msub> <mrow> <mi>d</mi> <mi>s</mi> <mi>i</mi> <mi>z</mi> <mi>e</mi> </mrow> <mrow> <mi>p</mi> <mi>x</mi> </mrow> </msub> </mrow> </semantics></math> produced a constant pointing error (green) that can be scaled to centimeters based on the calibration photos in (<b>C</b>). (<b>C</b>) An example calibration photo of participant’s hand lying on top of graph paper in order to calculate <math display="inline"><semantics> <mrow> <mi>h</mi> <mi>a</mi> <mi>n</mi> <msub> <mrow> <mi>d</mi> <mi>s</mi> <mi>i</mi> <mi>z</mi> <mi>e</mi> </mrow> <mrow> <mi>c</mi> <mi>m</mi> </mrow> </msub> </mrow> </semantics></math>.</p>
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<p>Graphical summary of the different tasks tested in Experiment 1.</p>
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<p>Examples of persons post stroke performing the pointing task. In Experiment 2, participants who had had a stroke sometimes could not extend the fingers of their target (hemiparetic) hand and were instructed to point to different landmarks on their hand depending on their capability. (<b>A</b>) Participant pointing to the fingertips while holding a foam pillow against the chest. (<b>B</b>) Participant pointing to the PIP joint while using an arm sling to hold his arm in a fixed position during the duration of the experiment. (<b>C</b>) Participant pointing to the MCP joint and using an arm sling.</p>
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<p>(<b>A</b>) Experimental setup for measuring finger proprioceptive error using the Crisscross assessments. For Crisscross, the FINGER robot moved the index and middle fingers in a crossing movement and participants were instructed to press a button with their other hand when they perceived them to be overlapped. The gray rectangle indicates the location of the opaque plastic divider used during the assessment to block the hand from view. (<b>B</b>) Example trajectories for the metacarpophalangeal (MCP) joint of the index (blue) and middle (black) fingers during Crisscross.</p>
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<p>Experiment 1 results. In this experiment we evaluated the pointing error of unimpaired young (<span class="html-italic">n</span> = 22) and older (<span class="html-italic">n</span> = 18) individuals in different tasks. (<b>A</b>) Two-dimensional representation of the target hand (in black) showing the mean and standard deviation across participants of the pointing endpoint (in colors). The plotted data are from the young group. (<b>B</b>) Pointing error for each task (black: mean and SD for younger participants, dark red: mean and SD for older participants). Colored points show the pointing error for individual users.</p>
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<p>Pointing error as a function of different factors in Experiment 1. (<b>A</b>) Visual condition (ANOVA, <span class="html-italic">p</span> &lt; 0.001). (<b>B</b>) Real or fake target hand (<span class="html-italic">p</span> &lt; 0.001). (<b>C</b>) Age (<span class="html-italic">p</span> = 0.005). (<b>D</b>) Distance from the target hand to the body (<span class="html-italic">p</span> &lt; 0.001). The error bars represent the standard deviation (SD) of the pointing errors.</p>
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<p>Further Analysis of Pointing Error from Experiment 1. (<b>A</b>) The effect of target hand conditions (real and fake) and visual condition (full, partial, and blindfolded), <span class="html-italic">p</span> &lt; 0.001 (<b>B</b>) The effect of visual condition (full, partial, and blindfolded) and age (young and older), <span class="html-italic">p</span> = 0.05. (<b>C</b>) The effect of target hand (real and fake) and age (young and older), <span class="html-italic">p</span> = 0.002. (<b>D</b>) The effect of visual condition (full, partial, and blindfolded) and age (young and older) for the real hand, <span class="html-italic">p</span> = 0.59. (<b>E</b>) The effect of visual condition (full, partial, and blindfolded) and age (young and older) for the fake hand, <span class="html-italic">p</span> = 0.09, with additional lines showing the effects of task order. (<b>F</b>) The effect of distance (target hand close to the body vs. target hand extended out from the body) and age (older and young), <span class="html-italic">p</span> &lt; 0.001. The error bars represent the standard deviation (SD) of the pointing errors.</p>
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<p>Results from Experiment 2. Proprioceptive pointing error was higher in persons who had experienced a stroke and was correlated with an independent, robot-based measure of their finger proprioception. (<b>A</b>) The pointing errors from Task 2 comparing the older and stroke groups. The stroke group had a significantly larger pointing error compared to the older group (<span class="html-italic">p</span> &lt; 0.001). The error bars represent the standard deviation (SD) of the pointing errors. (<b>B</b>) OpenPoint pointing error was moderately correlated with the Crisscross finger proprioception error angular error. Each scatter point represents a participant.</p>
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19 pages, 6343 KiB  
Article
Colletotrichum gloeosporioides Swiftly Manipulates the Transcriptional Regulation in Citrus sinensis During the Early Infection Stage
by Siyu Zhang, Xinyou Wang, Wei Zeng, Leijian Zhong, Xiaoyong Yuan, Zhigang Ouyang and Ruimin Li
J. Fungi 2024, 10(11), 805; https://doi.org/10.3390/jof10110805 - 20 Nov 2024
Viewed by 253
Abstract
Citrus spp. represent an economically important fruit tree crop worldwide. However, molecular mechanisms underlying the interaction between citrus and the Colletotrichum gloeosporioides remain largely unexplored. In this study, we analyzed the physiological and transcriptomic changes in Citrus sinensis at different stages of incubation [...] Read more.
Citrus spp. represent an economically important fruit tree crop worldwide. However, molecular mechanisms underlying the interaction between citrus and the Colletotrichum gloeosporioides remain largely unexplored. In this study, we analyzed the physiological and transcriptomic changes in Citrus sinensis at different stages of incubation with C. gloeosporioides. The results indicated that C. gloeosporioides infection rapidly triggered necrosis in the epicarp of C. sinensis fruits, decreased the total flavonoid contents, and suppressed the activity of catalase, peroxidase, and superoxide dismutase enzymes. Upon inoculation with C. gloeosporioides, there were 4600 differentially expressed genes (DEGs) with 1754 down-regulated and 2846 up-regulated after six hours, while there were only 580 DEGs with 185 down-regulated and 395 up-regulated between six and twelve-hours post-inoculation. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that the DEGs, which exhibited consistent up-regulation, were associated with metabolic processes and stress responses. Through Weighted Gene Co-Expression Network Analysis, 11 key genes have been identified that could potentially play a role in the transcriptional regulation of this process, including the transcription factor bHLH189. Furthermore, the infection of C. gloeosporioides had a notable effect on both the flavonoid metabolism and the metabolic pathways related to reactive oxygen species. Our findings help to understand the interaction between citrus and C. gloeosporioides and unveil how new insights into how C. gloeosporioides circumvents citrus defense mechanisms. Full article
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<p>The fruit phenotype changes of <span class="html-italic">Citrus sinensis</span> after inoculation with <span class="html-italic">Colletotrichum gloeosporioides</span> at different stages. (<b>A</b>) Control samples. (<b>B</b>) Samples of 6 h post-inoculation. (<b>C</b>) Samples of 12 h post-inoculation. (<b>D</b>) Samples of 24 h post-inoculation. The white dashed lines and black dashed circles indicate the inoculation regions.</p>
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<p>The alterations in physiological indicators of <span class="html-italic">Citrus sinensis</span> fruit after inoculation with <span class="html-italic">Colletotrichum gloeosporioides</span> at different stages. (<b>A</b>) Content of total flavonoid. (<b>B</b>) Catalase (CAT) activity. (<b>C</b>) Peroxidase (POD) activity. (<b>D</b>) Superoxide dismutase (SOD) activity. The letter ‘a’ ‘b’ ‘c’ on the bar denote a statistically significant discrepancy with a <span class="html-italic">p</span>-value inferior to 0.05.</p>
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<p>Analysis of differentially expressed genes (DEGs) and their distribution on chromosomes of <span class="html-italic">Citrus sinensis</span> during <span class="html-italic">Colletotrichum gloeosporioides</span> infection. (<b>A</b>) DEGs in <span class="html-italic">C. sinensis</span> fruits at various incubation stages of <span class="html-italic">C. gloeosporioides</span>. (<b>B</b>) Distribution of DEGs between 6 h post-inoculation and control samples on each chromosome of <span class="html-italic">C. sinensis</span>. (<b>C</b>) Distribution of DEGs between 12 h post-inoculation and control samples on each chromosome of <span class="html-italic">C. sinensis</span>. (<b>D</b>) Distribution of DEGs between 12 h and 6 h post-inoculation on each chromosome of <span class="html-italic">C. sinensis</span>. Cg0h, Cg6h, and Cg12h refer to control samples and samples of 6- and 12 h post-inoculation with <span class="html-italic">C. gloeosporioides</span>, respectively. Log2FC represents the log2 of the fold change in the expression value.</p>
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<p>Cluster and GO enrichment analysis of DEGs of <span class="html-italic">Citrus sinensis</span> during <span class="html-italic">Colletotrichum gloeosporioides</span> infection. (<b>A</b>) Venn diagram of DEGs across ‘Cg6h vs. Cg0h’, ‘Cg12h vs. Cg0h’, and ‘Cg12h vs. Cg6h’. (<b>B</b>) Expression profiles of DEGs. (<b>C</b>) Cluster analysis of DEGs. (<b>D</b>) GO enrichment analysis of DEGs in cluster 1. (<b>E</b>) GO enrichment analysis of DEGs in cluster 6.</p>
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<p>KEGG enrichment analysis of up-regulated DEGs of <span class="html-italic">Citrus sinensis</span> during <span class="html-italic">Colletotrichum gloeosporioides</span> infection. (<b>A</b>) KEGG enrichment analysis of DEGs in cluster 1. (<b>B</b>) KEGG enrichment analysis of DEGs in cluster 6.</p>
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<p>Identifying key modules of gene sets from <span class="html-italic">Citrus sinensis</span> associated with <span class="html-italic">Colletotrichum gloeosporioides</span> infection by WGCNA. (<b>A</b>) Cluster genes into various modules. (<b>B</b>) The correlation analysis between modules and incubation stages of <span class="html-italic">C. gloeosporioides</span>. The numbers in the rectangular columns show the correlation coefficient and <span class="html-italic">p</span> value. The enlarged labels indicate significant modules. The correlation analysis between module membership and gene significance in (<b>C</b>) green module, (<b>D</b>) red module, (<b>E</b>) blue module, and (<b>F</b>) grey module.</p>
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<p>Co-expression networks of each significant module. (<b>A</b>) Green module. (<b>B</b>) Red module. (<b>C</b>) Blue module. (<b>D</b>) Grey module. Cg6h and Cg12h indicate samples of 6- and 12 h post-inoculation with <span class="html-italic">C. gloeosporioides</span>, respectively. The core genes were annotated in the networks.</p>
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<p>Expression profiles of differential expressed flavonoid metabolism-associated genes of <span class="html-italic">Citrus sinensis</span> during <span class="html-italic">Colletotrichum gloeosporioides</span> infection. Cg0h, Cg6h, and Cg12h refer to control samples and samples of 6- and 12 h post-inoculation with <span class="html-italic">C. gloeosporioides</span>, respectively. The numbers following Cg0h, Cg6h, and Cg12h indicate different biological replicates. Red arrows indicate genes that are up-regulated, while blue arrows represent genes that are down-regulated. The numbers beside the arrows indicate the number of DEGs. PAL, phenylalanine ammonia lyase. C4H, cinnamate-4-hydroxylase. 4CL, 4-coumarate:CoA ligase. CHS, chalcone synthase. CHI, chalcone isomerase. F3H, flavanone 3-hydroxylase.</p>
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<p>Expression profiles of differential expressed ROS metabolism-associated genes of <span class="html-italic">Citrus sinensis</span> during <span class="html-italic">Colletotrichum gloeosporioides</span> infection. Cg0h, Cg6h, and Cg12h refer to control samples and samples of 6- and 12 h post-inoculation with <span class="html-italic">C. gloeosporioides</span>, respectively. The numbers following Cg0h, Cg6h, and Cg12h indicate different biological replicates. Red arrows indicate genes that are up-regulated, while blue arrows represent genes that are down-regulated. The numbers beside the arrows indicate the number of DEGs. RBOH, NADPH oxidase. SOD, superoxide dismutase. CAT, catalase. PrxR, peroxiredoxin. Trx, thioredoxin. APX, ascorbate peroxidase. MDAR, monodehydroascorbate reductase. DHAR, dehydroascorbate reductase. GLR, glutaredoxin.</p>
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<p>Modules of <span class="html-italic">Colletotrichum gloeosporioides</span> swiftly manipulate the transcriptional regulation in <span class="html-italic">Citrus sinensis</span> during the early infection stage. The infection by <span class="html-italic">C. gloeosporioides</span> hinders both ROS scavenging and flavonoid metabolism within <span class="html-italic">C. sinensis</span>. This may be due to either the defensive response of <span class="html-italic">C. sinensis</span> being triggered by the infection, disrupting the ROS scavenging and regulation of flavonoid metabolism, or the secretion of effectors by <span class="html-italic">C. gloeosporioides</span> into the plant cells, directly or indirectly affecting the ROS scavenging and regulation of flavonoid metabolism. CW, cell wall. PM, plasma membrane. h, haustorium. n, nuclear.</p>
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20 pages, 4455 KiB  
Article
Forecasting Raw Material Yield in the Tanning Industry: A Machine Learning Approach
by Ismael Cristofer Baierle, Leandro Haupt, João Carlos Furtado, Eluza Toledo Pinheiro and Miguel Afonso Sellitto
Forecasting 2024, 6(4), 1078-1097; https://doi.org/10.3390/forecast6040054 - 20 Nov 2024
Viewed by 375
Abstract
This study presents an innovative machine learning (ML) approach to predicting raw material yield in the leather tanning industry, addressing a critical challenge in production efficiency. Conducted at a tannery in southern Brazil, the research leverages historical production data to develop a predictive [...] Read more.
This study presents an innovative machine learning (ML) approach to predicting raw material yield in the leather tanning industry, addressing a critical challenge in production efficiency. Conducted at a tannery in southern Brazil, the research leverages historical production data to develop a predictive model. The methodology encompasses four key stages: data collection, processing, prediction, and evaluation. After rigorous analysis and refinement, the dataset was reduced from 16,046 to 555 high-quality records. Eight ML models were implemented and evaluated using Orange Data Mining software, version 3.38.0, including advanced algorithms such as Random Forest, Gradient Boosting, and neural networks. Model performance was assessed through cross-validation and comprehensive metrics, including Mean Absolute Error (MAE), Root Mean Squared Error (RMSE), Mean Squared Error (MSE), and Coefficient of Determination (R2). The AdaBoost algorithm emerged as the most accurate predictor, achieving impressive results with an MAE of 0.042, MSE of 0.003, RMSE of 0.057, and R2 of 0.331. This research demonstrates the significant potential of ML techniques in enhancing raw material yield forecasting within the tanning industry. The findings contribute to more efficient forecasting processes, aligning with Industry 4.0 principles and paving the way for data-driven decision-making in manufacturing. Full article
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<p>Initial methodological steps.</p>
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<p>Configuration of parameters used in each model.</p>
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<p>Method developed to generate the prediction model.</p>
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<p>Flowchart of the relationship between leather condition and type of PO.</p>
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<p>Removal of outliers before and after using the IQR rule.</p>
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<p>Filtering the samples based on the filters applied.</p>
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<p>Modeling in Orange software.</p>
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11 pages, 3628 KiB  
Article
Facile Preparation of High-Performance Polythiophene Derivative and Effect of Torsion Angle Between Thiophene Rings on Electrochromic Color Change
by Qingfu Guo, Chao Sun, Yiran Li, Kaoxue Li and Xishi Tai
Molecules 2024, 29(22), 5477; https://doi.org/10.3390/molecules29225477 - 20 Nov 2024
Viewed by 266
Abstract
The electrochromic phenomenon of conducting polymer is mainly dominated by the π-π* band transition. The π conjugation is influenced by the coplanarity between polymer units, deviations from which can lead to an increased ionization potential and band gap values. In order to investigate [...] Read more.
The electrochromic phenomenon of conducting polymer is mainly dominated by the π-π* band transition. The π conjugation is influenced by the coplanarity between polymer units, deviations from which can lead to an increased ionization potential and band gap values. In order to investigate the effect of plane distortion angle on electrochromic color in the main chain structure of polymerization, high-performance poly(3,3′-dimethyl-2,2′-bithiophene) (PDMeBTh) with a large plane distortion angle is successfully synthesized in boron trifluoride diethyl etherate (BFEE) by the electrochemical anodic oxidation method. The electrochemical and thermal properties of PDMeBTh prepared from BFEE and ACN/TBATFB are compared. The electrochromic properties of PDMeBTh are systematically investigated. The PDMeBTh shows a different color change (orange-yellow in the neutral state) compared to poly (3-methylthiophene) (light-red in the neutral state) due to the large torsion angle between thiophene rings of the main polymer chain. The optical contrast, response time, and coloring efficiency (CE) of the prepared PDMeBTh are also studied, which shows good electrochromic properties. For practical applications, an electrochromic device is fabricated by the PDMeBTh and PEDOT. The color of the device can be reversibly changed between orange-yellow and dark blue. The light contrast of the device is 27% at 433 nm and 61% at 634 nm. The CE value of the device is 403 cm2 C−1 at 433 nm and 577 cm2 C−1 at 634 nm. The constructed device also has good open circuit memory and electrochromic stability, showing good potential for practical applications. Full article
(This article belongs to the Section Macromolecular Chemistry)
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<p>(<b>A</b>) LSV curves of DMeBTh in (a) BFEE and (b) ACN/TBATFB; (<b>B</b>) CVs of DMeBTh in BFEE.</p>
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<p>CV curves of PDMeBTh in (<b>A</b>,<b>B</b>) ACN/TBATFB and (<b>C</b>,<b>D</b>) concentrated sulfuric acid under scan rates of 25, 50, 100, 150, 200, and 250 mV s<sup>−1</sup>; the PDMeBTh was prepared in (<b>A</b>,<b>C</b>) BFEE and (<b>B</b>,<b>D</b>) ACN/TBATFB.</p>
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<p>SEM images of the PDMeBTh prepared in (<b>A</b>) BFEE and (<b>B</b>) ACN/TBATFB; TG (a) and DTG (b) curves of the PDMeBTh prepared in (<b>C</b>) BFEE and (<b>D</b>) ACN/TBATFB.</p>
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<p>(<b>A</b>) Spectroelectrochemistry of the PDMeBTh in ACN/TBATFB under different potentials (V): (a) –1.0, (b) −0.6, (c) −0.4, (d) −0.2, (e) 0.0, (f) 0.2, (g) 0.3, (h) 0.4, (i) 0.5, (j) 0.6, (k) 0.7, and (l) 0.8; (<b>B</b>) the molecular structure and photographs of PDMeBTh in oxidation and reduction states; (<b>C</b>,<b>D</b>) the timed absorption spectra of PDMeBTh at 450 nm and 740 nm.</p>
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<p>(<b>A</b>) The structure of the constructed ECD and its color at different voltages; (<b>B</b>) the spectroelectrochemistry of PDMeBTh/PEDOT device under different voltages (V): (a) –1.0, (b) −0.8, (c) −0.6, (d) −0.4, (e) −0.2, (f) 0.2, (g) 0.4, (h) 0.6 (i) 0.8, (j) 1.0, (k) 1.2, (l) 1.4, (m) 1.6, (n) 1.8, and (o) 2.0; (<b>C</b>,<b>D</b>) the timed absorption spectra of the ECD at 433 nm and 634 nm.</p>
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<p>Open circuit memory of ECD at (<b>A</b>) 433 nm and (<b>B</b>) 634 nm, with applied potentials of −1.0 V and +2.0 V.</p>
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<p>(<b>A</b>,<b>B</b>) The timed absorption spectra of the ECD for 1–2 cycles and 1000–1001 cycles at 433 nm and 634 nm; (<b>C</b>) the redox stability of the ECD.</p>
Full article ">Scheme 1
<p>The structure of 3,4′-dimethyl-2,2′-bithiophene (head-to-head) and 3,3′-dimethyl-2,2′-bithiophene (head-to-tail).</p>
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