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Search Results (1,986)

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Keywords = food imaging

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15 pages, 3454 KiB  
Article
Three-Dimensional Characterization of Potatoes Under Different Drying Methods: Quality Optimization for Hybrid Drying Approach
by Yinka Sikiru, Jitendra Paliwal and Chyngyz Erkinbaev
Foods 2024, 13(22), 3633; https://doi.org/10.3390/foods13223633 - 14 Nov 2024
Viewed by 255
Abstract
The quality evaluation of processed potatoes is vital in the food industry. In this study, the effect of three different drying methods on the post-processing quality of potatoes utilizing 4, 8, 12, and 16 h of freeze drying (FD), infrared drying (ID), and [...] Read more.
The quality evaluation of processed potatoes is vital in the food industry. In this study, the effect of three different drying methods on the post-processing quality of potatoes utilizing 4, 8, 12, and 16 h of freeze drying (FD), infrared drying (ID), and oven drying (OD) was investigated. The impact of the drying methods on the potato’s microstructure was analyzed and quantified using 3D X-ray micro-computed tomography images. A new Hybrid Quality Score Evaluator (HQSE) was introduced and used to assess the Quality Index (QI) and Specific Energy Consumption Index (SECI) across various drying methods and durations. Mathematical models were developed to predict the optimal drying method. FD showed significantly higher (p < 0.05) colour retention, rehydration ratio, and total porosity, with minimal shrinkage, although it had higher energy consumption. ID had the shortest drying time, followed by OD and FD. The optimization showed that for FD, the optimal time of 5.78 h increased QI by 9.7% and SECI by 30.6%. The mathematical models could accurately predict the QI and SECI under different drying methods, balancing quality preservation with energy efficiency. The findings suggest that a hybrid drying system could optimize potato quality and energy consumption. Full article
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<p>Flow chart of the image processing and analysis.</p>
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<p>The morphological changes in potatoes post drying by freeze drying (FD), infrared drying (ID), and oven drying (OD): (<b>a</b>)—top view of 3D images of potatoes after 3 mm penetration, showing changes in pores after drying; (<b>b</b>)—isometric view of potato samples dried for 16 h with the transparency of the fresh sample reduced to 3% and the dried sample to 97%.</p>
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<p>A comparative analysis of potato drying dynamics: (<b>a</b>) changes in total porosity with time across different drying methods; (<b>b</b>) the effect of different drying methods on the rehydration ratio of dried potato; (<b>c</b>–<b>e</b>) drying kinematics curves of potato under three drying methods.</p>
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<p>Quality and energy consumption analysis of potato drying processes: (<b>a</b>) changes in the quality index; (<b>b</b>) specific energy consumption index with time across different drying methods; (<b>c</b>) Pareto fronts of drying methods for optimizing quality and energy efficiency in potato drying; and a stacked bar graph illustrating the composite quality score for potatoes subjected to (<b>d</b>) freeze drying, (<b>e</b>) infrared drying, and (<b>f</b>) oven drying across various drying times. Each segment represents the weighted contribution of individual quality parameters—colour change, hardness, total porosity, area and volume shrinkage ratio, rehydration ratio, moisture ratio, and drying rate—to the composite quality index for each drying method.</p>
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<p>Comparative validation of predictive models against measured data showcasing (<b>a</b>) freeze drying; (<b>b</b>) infrared drying; (<b>c</b>) oven drying methods. Each subplot illustrates the correlation between the predicted quality index (<b>d</b>–<b>f</b>), QI, and specific energy consumption index, SECI, against their respective experimental outcomes.</p>
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17 pages, 1181 KiB  
Article
Do Consumers’ Perceived Attributes and Normative Factors Affect Acceptance Behavior Towards Eco-Friendly Self-Driving Food Delivery Services? The Moderating Role of Country Development Status
by Kyuhyeon Joo, Heather Markham Kim and Jinsoo Hwang
Sustainability 2024, 16(22), 9918; https://doi.org/10.3390/su16229918 - 14 Nov 2024
Viewed by 205
Abstract
The advent of self-driving technology marks a significant milestone in the evolution of modern transportation and logistics services. More importantly, self-driving food delivery services are expected to play a significant role in environmental protection by operating on batteries instead of the traditional gasoline. [...] Read more.
The advent of self-driving technology marks a significant milestone in the evolution of modern transportation and logistics services. More importantly, self-driving food delivery services are expected to play a significant role in environmental protection by operating on batteries instead of the traditional gasoline. The current study examines the relationship between perceived attributes, image, normative factors, and behavioral intentions in the context of eco-friendly self-driving food delivery services. The study deepens the framework by identifying the moderating role of country development status. The study gathered samples from 313 panels in South Korea, a developed country, and 315 respondents in Mongolia, a developing country. The results of the South Korean dataset showed that two types of perceived attributes, perceived innovativeness and perceived risk significantly affect image, which in turn leads to the formation of behavioral intentions. Normative factors, such as subjective norms and personal norms, also positively affect behavioral intentions, and subjective norms increase personal norms. The results of the Mongolian dataset indicated that all paths are statistically supported. Lastly, the moderating role of the country development status was found in the relationship between (1) perceived innovativeness and perceived risk, (2) subjective norms and personal norms, and (3) subjective norms and behavioral intentions. Full article
(This article belongs to the Special Issue Sustainable Consumption and Circular Economy)
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<p>Proposed conceptual model. Notes: H = Hypothesis, the normal arrows present the hypotheses regarding causal relationships, and the bold arrows present the hypotheses regarding moderating effects.</p>
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<p>Standardized theoretical path coefficients. Notes: NFI = Normed fit index, CFI = Comparative fit index, TLI = Tucker–Lewis index, RMSEA = Root mean square error of approximation. Unmarked values are for Korean consumers, underlined values are for Mongolian consumers, and * <span class="html-italic">p</span> &lt; 0.05.</p>
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<p>Screenshots from the video. Source: Lucchetti [<a href="#B108-sustainability-16-09918" class="html-bibr">108</a>].</p>
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13 pages, 3187 KiB  
Article
Enhancing Medium-Chain Fatty Acid Delivery Through Bigel Technology
by Manuela Machado, Eduardo M. Costa, Sara Silva, Sérgio C. Sousa, Ana Maria Gomes and Manuela Pintado
Gels 2024, 10(11), 738; https://doi.org/10.3390/gels10110738 - 14 Nov 2024
Viewed by 225
Abstract
This study presents the development and characterization of medium-chain fatty acid (MCFA)-loaded bigels, using coconut oil as the MCFA source. The bigels exhibited high oil binding capacity, ranging from 87% to 98%, effectively retaining MCFAs within the matrix, with lauric acid (C12) being [...] Read more.
This study presents the development and characterization of medium-chain fatty acid (MCFA)-loaded bigels, using coconut oil as the MCFA source. The bigels exhibited high oil binding capacity, ranging from 87% to 98%, effectively retaining MCFAs within the matrix, with lauric acid (C12) being the main component detected within the bigels at 178.32 ± 0.10 mg/g. Physicochemical analysis, including FTIR and scanning electron microscopy, confirmed stable fatty acid incorporation and a cohesive, smooth structure. The FTIR spectra displayed O-H and C=O stretching vibrations, indicating hydrogen bonding within the matrix, while the SEM images showed uniform lipid droplet distribution with stable phase separation. Thermal stability tests showed that the bigels were stable for 5 days at 50 °C, with oil retention and structural integrity unchanged. Rheological testing indicated a solid-like behavior, with a high elastic modulus (G′) that consistently exceeded the viscous modulus (G″), which is indicative of a strong internal structure. In simulated gastrointestinal digestion, the bigels achieved significantly higher MCFA retention than the pure oil, particularly in the gastric phase, with recovery percentages of 38.1% for the bigels and 1.7% for the oil (p < 0.05), suggesting enhanced bioavailability. Cell-based cytotoxicity assays showed low cytotoxicity, and permeability testing in a co-culture Caco-2/HT29-MTX model revealed a controlled, gradual MCFA release, with approximately 10% reaching the basolateral side over 6 h. These findings highlight MCFA-loaded bigels as a promising platform for nutraceutical applications; they provided stability, safety, and controlled MCFA release, with significant potential for functional foods aimed at enhancing fatty acid bioavailability. Full article
(This article belongs to the Section Gel Processing and Engineering)
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Graphical abstract

Graphical abstract
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<p>(<b>A</b>): FTIR spectra of bigels and their components (coconut oil, geleol, CMC, and tween 80). (<b>B</b>): Identification of the major functional groups in oil and bigel FTIR spectra.</p>
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<p>The microstructure of bigels using SEM technology.</p>
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<p>Impact of temperature on bigel’s MCFA amount (<b>A</b>) and oil binding capacity (<b>B</b>) during the 5 days of storage at 50 °C. ns means no significant differences (<span class="html-italic">p</span> &gt; 0.05), * means significant differences (<span class="html-italic">p</span> &lt; 0.05), and *** means significant differences (<span class="html-italic">p</span> &lt; 0.001) and **** means significant differences (<span class="html-italic">p</span> &lt; 0.0001).</p>
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<p>Rheological properties of bigels during thermal stability (T0—day 0, T1—day 1, T2—day 2, T3—day 3, T4—day 5); (<b>A</b>) elastic modulus G′; (<b>B</b>) viscous modulus G″; (<b>C</b>) complex viscosity η*; (<b>D</b>) instantaneous viscosity η′.</p>
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<p>Recovery percentages after gastrointestinal tract: saturated (<b>A</b>), monounsaturated (<b>B</b>), polyunsaturated (<b>C</b>), and medium-chain fatty acids (<b>D</b>). The release profile of MCFAs during gastrointestinal tract (<b>E</b>). * means significant differences at (<span class="html-italic">p</span> &lt; 0.05), ** means significant differences (<span class="html-italic">p</span> &lt; 0.01), *** means significant differences (<span class="html-italic">p</span> &lt; 0.001) and **** means significant differences (<span class="html-italic">p</span> &lt; 0.0001).</p>
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<p>Effect of digested oil and bigel upon Caco-2 and HT29-MTX metabolism. ns means no significant differences (<span class="html-italic">p</span> &gt; 0.05). The dotted line represents the 30% cytotoxicity limit, as defined by the ISO 10993-5 [<a href="#B26-gels-10-00738" class="html-bibr">26</a>].</p>
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<p>(<b>A</b>): Membrane stability as TEER (%) and (<b>B</b>): MCFA permeability over 6 h. ns means no significant differences, * means significant differences (<span class="html-italic">p</span> &lt; 0.05) and *** means significant differences (<span class="html-italic">p</span> &lt; 0.001).</p>
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<p>Schematic representation of bigel production.</p>
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12 pages, 643 KiB  
Article
The Dynamic Impacts of Public Perceptions of Fast-Food Products with Nutrition Facts on Fast-Food Consumption
by Po-Lin Pan, Manu Bhandari and Li Zeng
Sustainability 2024, 16(22), 9913; https://doi.org/10.3390/su16229913 - 14 Nov 2024
Viewed by 448
Abstract
Although most American eat at a fast-food restaurant 1 to 3 times a week, they would realize that fast food consumption is highly associated with chronic diseases and generates negative impacts on their health. As fast-food consumers become more health-conscious, fast-food brands strive [...] Read more.
Although most American eat at a fast-food restaurant 1 to 3 times a week, they would realize that fast food consumption is highly associated with chronic diseases and generates negative impacts on their health. As fast-food consumers become more health-conscious, fast-food brands strive to build a more health-oriented image on their fast-food products. Thus, this study proposes a conceptual model that aims to examine direct and indirect impacts of consumers’ BMI, self-efficacy, perceived brand trust, and brand commitment on their fast-food consumption. An online survey using Amazon Mechanical Turk is conducted with a total of 484 female and 380 male participants included in the final analysis. Results show that the mediating effects of self-efficacy, brand trust in, and brand commitment with the fast-food product with nutrition facts are significantly generated on consumers’ fast-food consumption. Moreover, indirect effects are found on consumers’ fast-food consumption via the nexus of their self-efficacy, brand trust, and brand commitment. The study also offers practical insights into the impact of health-conscious consumers’ brand perceptions on their fast-food consumption. Full article
(This article belongs to the Special Issue Food, Supply Chains, and Sustainable Development—Second Edition)
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<p>Conceptual model with self-efficacy, brand trust, and brand commitment as mediators.</p>
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<p>Statistical model with self-efficacy, brand trust, and brand commitment as mediators. Note: ** <span class="html-italic">p</span> &lt; 0.01; * <span class="html-italic">p</span> &lt; 0.05.</p>
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14 pages, 6740 KiB  
Article
Detection of Rice Leaf Folder in Paddy Fields Based on Unmanned Aerial Vehicle-Based Hyperspectral Images
by Shanshan Feng, Shun Jiang, Xuying Huang, Lei Zhang, Yangying Gan, Laigang Wang and Canfang Zhou
Agronomy 2024, 14(11), 2660; https://doi.org/10.3390/agronomy14112660 - 12 Nov 2024
Viewed by 350
Abstract
Pest infestations significantly impact rice production and threaten food security. Remote sensing offers a vital tool for the non-destructive, rapid detection of rice pests. Existing studies often focus on laboratory conditions at the leaf level, limiting their applicability for precise pesticide application. Therefore, [...] Read more.
Pest infestations significantly impact rice production and threaten food security. Remote sensing offers a vital tool for the non-destructive, rapid detection of rice pests. Existing studies often focus on laboratory conditions at the leaf level, limiting their applicability for precise pesticide application. Therefore, this study aimed to develop a method for detecting rice pests (rice leaf folders) in paddy fields based on unmanned aerial vehicle (UAV) hyperspectral data. Firstly, a UAV imaging system collected hyperspectral images of rice plants in both the jointing and heading stages. A total of 222 field plots for investigating rice leaf folders was established during these two periods. Secondly, 23 vegetation indices were calculated as candidates for identifying rice pests. Then, hyperspectral data and field investigation data from the jointing stage were used to construct a machine learning (extreme gradient boosting, XGBoost) algorithm for detecting rice pests. The results showed that the XGBoost model exhibited the best performance when eight vegetation indices were considered as the selected input features for model construction: the Red-edge Normalized Difference Vegetation Index (red-edge NDVI), Structure Insensitive Pigment Index (SIPI), Enhanced Vegetation Index (EVI), Atmospherically Resistant Vegetation Index (ARVI), Soil-Adjusted Vegetation Index (SAVI), Red-edge Chlorophyll Index (CIred-edge), Pigment-Specific Simple Ratio680 (PSSR680), and Carotenoid Reflectance Index700 (CPI700). The training and testing accuracies reached 87.46% and 86%, respectively. Furthermore, the heading stage application confirmed the model’s feasibility. Thus, the XGBoost model with input features of eight vegetation indices provides an effective and reliable method for detecting rice leaf folders, supporting real-time, precise pesticide use in rice cultivation. Full article
(This article belongs to the Section Pest and Disease Management)
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<p>Experimental site of the paddy field.</p>
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<p>Technical flow of this study.</p>
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<p>Spectral reflectance of healthy rice and infested rice: (<b>A</b>) 28<sup>th</sup> Sept.; (<b>B</b>) 25<sup>th</sup> Oct.</p>
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<p>The contribution ranking of different features.</p>
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<p>The accuracy for 10-fold cross-validation in the model training process.</p>
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<p>Spatial distribution of rice pest infestations: (<b>A</b>) 28<sup>th</sup> Sept.; (<b>B</b>) 25<sup>th</sup> Oct.</p>
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20 pages, 10441 KiB  
Article
Proto-DS: A Self-Supervised Learning-Based Nondestructive Testing Approach for Food Adulteration with Imbalanced Hyperspectral Data
by Kunkun Pang, Yisen Liu, Songbin Zhou, Yixiao Liao, Zexuan Yin, Lulu Zhao and Hong Chen
Foods 2024, 13(22), 3598; https://doi.org/10.3390/foods13223598 - 11 Nov 2024
Viewed by 545
Abstract
Conventional food fraud detection using hyperspectral imaging (HSI) relies on the discriminative power of machine learning. However, these approaches often assume a balanced class distribution in an ideal laboratory environment, which is impractical in real-world scenarios with diverse label distributions. This results in [...] Read more.
Conventional food fraud detection using hyperspectral imaging (HSI) relies on the discriminative power of machine learning. However, these approaches often assume a balanced class distribution in an ideal laboratory environment, which is impractical in real-world scenarios with diverse label distributions. This results in suboptimal performance when less frequent classes are overshadowed by the majority class during training. Thus, the critical research challenge emerges of how to develop an effective classifier on a small-scale imbalanced dataset without significant bias from the dominant class. In this paper, we propose a novel nondestructive detection approach, which we call the Dice Loss Improved Self-Supervised Learning-Based Prototypical Network (Proto-DS), designed to address this imbalanced learning challenge. The proposed amalgamation mitigates the label bias on the most frequent class, further improving robustness. We validate our proposed method on three collected hyperspectral food image datasets with varying degrees of data imbalance: Citri Reticulatae Pericarpium (Chenpi), Chinese herbs, and coffee beans. Comparisons with state-of-the-art imbalanced learning techniques, including the Synthetic Minority Oversampling Technique (SMOTE) and class-importance reweighting, reveal our method’s superiority. Notably, our experiments demonstrate that Proto-DS consistently outperforms conventional approaches, achieving the best average balanced accuracy of 88.18% across various training sample sizes, whereas the Logistic Model Tree (LMT), Multi-Layer Perceptron (MLP), and Convolutional Neural Network (CNN) approaches attain only 59.42%, 60.38%, and 66.34%, respectively. Overall, self-supervised learning is key to improving imbalanced learning performance and outperforms related approaches, while both prototypical networks and the Dice loss can further enhance classification performance. Intriguingly, self-supervised learning can provide complementary information to existing imbalanced learning approaches. Combining these approaches may serve as a potential solution for building effective models with limited training data. Full article
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Graphical abstract

Graphical abstract
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<p>Figures of the imbalanced food products in the datasets: (<b>a</b>–<b>d</b>) samples from the chenpi dataset, (<b>e</b>,<b>f</b>) samples from the coffee bean dataset, and (<b>g</b>,<b>h</b>) samples from the Chinese herbs dataset.</p>
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<p>Figures of the imbalanced food products in the datasets: (<b>a</b>–<b>d</b>) samples from the chenpi dataset, (<b>e</b>,<b>f</b>) samples from the coffee bean dataset, and (<b>g</b>,<b>h</b>) samples from the Chinese herbs dataset.</p>
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<p>Training process of Proto-DS using spectral prototypical contrastive learning and fine-tuning with Dice loss to improve the prototypical network. For simplicity, we use the coffee bean dataset as an example. Blue line: data flow of unknown new incoming samples. Red line:the data flow of the positive samples (majority class). Yellow line: the data flow of the negative samples (minority class).</p>
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<p>The prototypical network with various available samples for training. The blue color indicates that the object is labeled as an authentic sample, while the green color indicates that the object is labeled as counterfeit. The light blue and light green data points denote the training samples in the embeddings. whereas the dark blue and dark green data points indicate the prototypes for authentic <math display="inline"><semantics> <msub> <mi mathvariant="bold-italic">c</mi> <mn>1</mn> </msub> </semantics></math> and counterfeit <math display="inline"><semantics> <msub> <mi mathvariant="bold-italic">c</mi> <mn>2</mn> </msub> </semantics></math>, respectively. The white circle indicates the unknown test data, while the dashed line represent the distance to the prototype vectors.</p>
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<p>The test process of Proto-DS. Light grey box: unknown new incoming samples during testing. Yellow box: training for Arabica coffee beans (majority class). Orange box: training for Robusta coffee beans (minority class).</p>
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<p>Class distributions for the coffee bean and Chinese herb datasets; dark blue denotes the majority class, light blue denotes the minority class, and percentage indicates the imbalance rate for the specific imbalance setting. Please note that the Chenpi dataset contains multiple minority classes, which we represent using different light blue colors.</p>
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<p>Visualisation spectra of each dataset. The straight lines are the averaged spectra among the particular classes, while the shaded area indicates the standard deviation of each class.</p>
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<p>Comparison with other state-of-the-art competitors in terms of balanced accuracy (B.Acc), M.F score (Macro-F score), macro-AUROC (M.AUROC), and macro-average precision (M.AP). <b>Proto-DS</b>: proposed method; <b>Conv-W</b>: CNN with class-reweighted cross entropy loss; <b>Conv-S</b>: CNN with SMOTE; <b>MLP-W</b>: MLP with class-reweighted cross entropy loss; <b>MLP-S</b>: MLP with SMOTE; <b>LMT-S</b>: logistic model tree with SMOTE and Adaboost.</p>
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<p>Comparison with state-of-the-art competitors in terms of sensitivity and specificity. Each figure summarizes the class-wise performance for all algorithms, while the rows corresponding to the different datasets.</p>
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<p>Results of the ablation study comparing different components in terms of balanced accuracy (B.Acc), M.F score (M.F1-score), macro-AUROC (M.AUROC), and macro-average precision (M.AP): <b>Proto-DS</b>, proposed method (blue straight line); <b>w/o D</b>, without applying Dice loss (blue dashed line); <b>w/o SSL</b>, without applying self-supervised learning (red straight line); <b>w/o SSL + D</b>, without self-supervised pretraining or Dice loss (red dashed line).</p>
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<p>Results of the ablation study comparing different components in terms of sensitivity (Sens.) and specificity (Spec.): <b>Proto-DS</b>, proposed method; <b>w/o D</b>, without applying Dice loss <b>w/o SSL</b>, without applying self-supervised learning; <b>w/o SSL + D</b>, without self-supervised pretraining or Dice loss.</p>
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<p>Visualization of the proposed model’s pixel-level probability for the corresponding class on various datasets. (<b>a</b>) Robusta (<b>b</b>) Arisaema (<b>c</b>) 5-Year-old Chenpi (<b>d</b>) 10-Year-old Chenpi (<b>e</b>) 15-Year-Old Chenpi. The rows represent particular samples from the minority class, while the columnsrepresent the raw image (Ground Truth), proposed method with all components (Proto-DS), and Proto-DS without particular components (w/o D, w/o SSL., w/o SSL + D). Brighter pixels indicate high probability on the corresponding class, while <b>darker pixels</b> indicate low probability on the corresponding class.</p>
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<p>Two-dimensional visualization of the learned feature space for the chenpi dataset with multiple settings: the rowsrepresent various minority training sample sizes (5/10/15/20), while the columns represent the proposed method with all components (Proto-DS) or without particular components (w/o S., w/o F. + S., w/o SSL + S., w/o SSL + F. + S.).</p>
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<p>Two-dimensional visualization of the learned feature space for the chenpi dataset with multiple settings: the rowsrepresent various minority training sample sizes (5/10/15/20), while the columns represent the proposed method with all components (Proto-DS) or without particular components (w/o S., w/o F. + S., w/o SSL + S., w/o SSL + F. + S.).</p>
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<p>Two-dimensional visualization of the learned feature space for the coffee bean dataset with multiple settings: the rows represent various minority training sample sizes (5/10/15/20), while the columns represents the proposed method with all components (Proto-DS) or without particular components (w/o S., w/o F. + S., w/o SSL + S., w/o SSL + F. + S.).</p>
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<p>Two-dimensional visualization of the learned feature space for the Chinese herbs dataset with multiple settings: the rows represent various minority training sample sizes (5/10/15/20), while the columns represent the proposed method with all components (Proto-DS) or without particular components (w/o S., w/o F. + S., w/o SSL + S., w/o SSL + F. + S.).</p>
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15 pages, 4389 KiB  
Article
Visualization of Moisture Content, Reducing Sugars, and Chewiness in Bread During Oral Processing Based on Hyperspectral Imaging Technology
by Xiaoyu Tian, Qin Fang, Xiaorui Zhang, Shanshan Yu, Chunxia Dai and Xingyi Huang
Foods 2024, 13(22), 3589; https://doi.org/10.3390/foods13223589 - 10 Nov 2024
Viewed by 572
Abstract
This study evaluated the differences in oral processing and texture perception of breads with varying compositions. The research investigated the dynamic changes in moisture content (MC), reducing sugars (RSs), and chewiness of the bolus formed from white bread (B0) and 50% whole-wheat bread [...] Read more.
This study evaluated the differences in oral processing and texture perception of breads with varying compositions. The research investigated the dynamic changes in moisture content (MC), reducing sugars (RSs), and chewiness of the bolus formed from white bread (B0) and 50% whole-wheat bread (B50) during oral processing. Hyperspectral imaging (HSI) combined with chemometric methods was used to establish quantitative prediction models for MC, RSs, and chewiness, and to create visual distribution maps of these parameters. The results showed that B0 had a higher moisture content and a faster hydration rate than B50 during the initial stages of oral processing, indicating greater hydrophilicity and ease of saliva wetting. Additionally, the uniformity of moisture distribution in the bolus of B0 was higher than that of B50. B50 exhibited significantly lower RSs content and poorer distribution uniformity compared to B0. The primary differences in chewiness between the two types of bread were observed during the early stages of oral processing, with B50 requiring more chewing effort initially. This study demonstrated that HSI technology can effectively monitor and elucidate the compositional changes in food particles during oral processing, providing new insights into bread texture perception and offering a scientific basis for improving bread processing and texture. Full article
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<p>Schematic representation of the oral processing of bread, measurement of physical and chemical properties, and hyperspectral images acquisition.</p>
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<p>Changes in moisture content (MC), reducing sugars (RSs), and chewiness during bread oral processing.</p>
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<p>Spectral profile of bread at different stages of oral processing. (<b>A</b>) Average raw spectral at different stages of oral processing. (<b>B</b>) Spectral preprocessed by SG algorithm. (<b>C</b>) Spectral preprocessed by GF algorithm. (<b>D</b>) Spectral preprocessed by normalize algorithm.</p>
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<p>Correlation between predicted and measured values for moisture content (MC), reducing sugars (RSs), and chewiness. The solid line represents the ideal prediction line, while the dashed line indicates the linear regression fit. (<b>A</b>) MC prediction, (<b>B</b>) RSs prediction, and (<b>C</b>) chewiness prediction.</p>
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<p>Distribution map of moisture content (MC), reducing sugars (RSs), and chewiness of two types of bread at different stages of oral processing.</p>
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<p>Correlation matrix of moisture content (MC), reducing sugars (RSs), chewiness, contrast MC, contrast RSs, and contrast chewiness during bread oral processing. * <span class="html-italic">p</span> ≤ 0.05, ** <span class="html-italic">p</span> ≤ 0.01.</p>
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16 pages, 1593 KiB  
Article
Effectiveness of Three Front-of-Pack Food Labels in Guiding Consumer Identification of Nutrients of Concern and Purchase Intentions in Kenya: A Randomized Controlled Trial
by Shukri F. Mohamed, Caroline H. Karugu, Samuel Iddi, Veronica Ojiambo, Caliph Kirui and Gershim Asiki
Nutrients 2024, 16(22), 3846; https://doi.org/10.3390/nu16223846 - 10 Nov 2024
Viewed by 467
Abstract
Background: Front-of-pack-labels (FOPLs) on packaged foods provide essential information to help consumers make informed dietary choices. However, evidence on their effectiveness, particularly in low- and middle-income countries like Kenya, is limited. Objective: This study assessed the effectiveness of three FOPLs in [...] Read more.
Background: Front-of-pack-labels (FOPLs) on packaged foods provide essential information to help consumers make informed dietary choices. However, evidence on their effectiveness, particularly in low- and middle-income countries like Kenya, is limited. Objective: This study assessed the effectiveness of three FOPLs in helping consumers identify nutrients of concern in packaged food products and influencing their purchase intention in Kenya. Methods: A total of 2198 shoppers from supermarkets in Nairobi, Mombasa, Kisumu, and Garissa were randomized into three groups: Red and Green Octagon label (RG), Red and Green Octagon with icons (RGI), and Black Octagon Warning label (WL). In the control phase, participants were shown unlabeled images of packaged foods, followed by questions. In the experimental phase, the same images were presented with one assigned FOPL, and participants responded again to the same set of questions. Differences in correct identification of nutrients of concern and changes in purchase intention were analyzed using frequency tables and Chi-Square tests, while modified Poisson regression assessed FOPL effectiveness. Results: FOPLs significantly improved correct nutrient identification and reduced the intention to purchase unhealthy foods, with the WL proving most effective. Conclusions: These findings highlight the potential of FOPLs, particularly the WL, as an effective regulatory tool for promoting healthier food choices in Kenya. Full article
(This article belongs to the Section Nutritional Policies and Education for Health Promotion)
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<p>Three front-of-pack-labels tested in Kenya.</p>
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<p>Study Flow Chart.</p>
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<p>Proportions of correct identification of nutrients of concern and unhealthiness of products.</p>
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<p>Reduced intention to purchase unhealthy foods by FOPL labels.</p>
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28 pages, 18631 KiB  
Article
Analysis of Paddy Field Changes (1989–2021) Using Landsat Images and Flooding-Assisted MLC in an Urbanizing Tropical Watershed, Vientiane, Lao PDR
by Iep Keovongsa, Atiqotun Fitriyah, Fumi Okura, Keigo Noda, Koshi Yoshida, Keoduangchai Keokhamphui and Tasuku Kato
Sustainability 2024, 16(22), 9776; https://doi.org/10.3390/su16229776 - 9 Nov 2024
Viewed by 693
Abstract
Paddy fields are essential for food security and sustaining global dietary needs, yet urban expansion often encroaches on agricultural lands. Analyzing paddy fields and land use/land cover changes over time using satellite images provides critical insights for sustainable food production and balanced urban [...] Read more.
Paddy fields are essential for food security and sustaining global dietary needs, yet urban expansion often encroaches on agricultural lands. Analyzing paddy fields and land use/land cover changes over time using satellite images provides critical insights for sustainable food production and balanced urban growth. However, mapping the paddy fields in tropical monsoon areas presents challenges due to persistent weather interference, monsoon-submerged fields, and a lack of training data. To address these challenges, this study proposed a flooding-assisted maximum likelihood classification (F-MLC) method. This approach utilizes accurate training datasets from intersecting flooded paddy field maps from the rainy and dry seasons, combined with the Automated Water Extraction Index (AWEI) to distinguish natural water bodies. The F-MLC method offers a robust solution for accurately mapping paddy fields and land use changes in challenging tropical monsoon climates. The classified images for 1989, 2000, 2013, and 2021 were produced and categorized into the following five major classes: urban areas, vegetation, paddy fields, water bodies, and other lands. The paddy field class derived for each year was validated using samples from various sources, contributing to the overall accuracies ranging from 83.6% to 90.4%, with a Kappa coefficient of between 0.80 and 0.88. The study highlights a significant decrease in paddy fields, while urban areas rapidly increased, replacing 23% of paddy fields between 1989 and 2021 in the watershed. This study demonstrates the potential of the F-MLC method for analyzing paddy fields and other land use changes over time in the tropical watershed. These findings underscore the urgent need for robust policy measures to protect paddy fields by clearly defining urban expansion boundaries, prioritizing paddy field preservation, and integrating these green spaces into urban development plans. Such measures are vital for ensuring a sustainable local food supply, promoting balanced urban growth, and maintaining ecological balance within the watershed. Full article
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<p>Location map of the study area.</p>
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<p>Referenced daily rainfall: 1989 (<b>a</b>), 2000 (<b>b</b>), 2013 (<b>c</b>), 2021 (<b>d</b>), and rice crop calendar in Laos (<b>e</b>) modified from FAO [<a href="#B65-sustainability-16-09776" class="html-bibr">65</a>].</p>
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<p>Methodological flowchart for detecting paddy fields and other classes.</p>
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<p>The initial paddy field maps for 1989 (<b>a</b>); 2000 (<b>b</b>); 2013 (<b>c</b>), and 2021 (<b>d</b>).</p>
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<p>The ground-truth data samples in the MHR watershed from field survey in 2022 and 2023.</p>
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<p>Spatial explanatory factors, road distance (m) for 2010 (<b>a</b>) and 2021 (<b>b</b>), and population density (person/km<sup>2</sup>) for 2015 (<b>c</b>) and the projection for 2020 (<b>d</b>).</p>
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<p>Classified LULC maps of the MHR watershed in 1989, 2000, 2013, and 2021.</p>
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<p>Change in Areas of LULC Classes in the MHR Watershed from 1989 to 2021.</p>
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<p>Spatial comparison and percent coverage: (<b>a</b>) 2000, (<b>b</b>) 2013, and (<b>c</b>) 2021 show spatial overlay of paddy fields between F-MLC and MAF’s maps; (<b>a1</b>–<b>c1</b>) provide detailed zoom-ins of the spatial overlay; (<b>a2</b>–<b>c2</b>) indicate the percent coverage of both maps in the watershed.</p>
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<p>Conversion trends of paddy fields in the MHR watershed (PF—paddy fields, UB—urban area, VE—vegetation, WB—water bodies, OL—other land (<b>left</b>). Demand and harvested rice (<b>right</b>).</p>
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<p>The boundary of the permitted areas for economic and urban development in Vientiane lies within the MHR watershed. “PF” refers to the paddy field areas.</p>
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<p>Observed points and Google Earth imagery: (<b>a1</b>–<b>c1</b>) show the zoomed-in sections of the classified image from 2021. (<b>a2</b>) shows that fallow paddy fields along the roadside were incorrectly classified as active paddy fields. (<b>b2</b>,<b>c2</b>) demonstrate the presence of rainwater storage ponds on paddy fields, as captured by Google Earth imagery.</p>
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<p>Detail of paddy field changes in peri-urban areas. The upper panels display imagery from Google Earth Pro for the clearest and most relevant time periods. The lower panels show the paddy fields affected by urban expansion from 2000 to 2021.</p>
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<p>Spatial conversion of paddy fields (PF) from 1989 to 2021: (<b>a</b>,<b>b</b>) provide zoomed-in views of PF conversion into urban areas near major roads.</p>
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<p>Band composition for identifying flooding/transplanting tone over the study area in July 1989.</p>
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29 pages, 2820 KiB  
Article
Investigating the Synergistic Effects of Carvacrol and Citral-Edible Polysaccharide-Based Nanoemulgels on Shelf Life Extension of Chalkidiki Green Table Olives
by Konstantinos Zaharioudakis, Constantinos E. Salmas, Nikolaos D. Andritsos, Areti A. Leontiou, Dimitrios Moschovas, Andreas Karydis-Messinis, Eleni Triantafyllou, Apostolos Avgeropoulos, Nikolaos E. Zafeiropoulos, Charalampos Proestos and Aris E. Giannakas
Gels 2024, 10(11), 722; https://doi.org/10.3390/gels10110722 - 8 Nov 2024
Viewed by 793
Abstract
Modern bioeconomy and sustainability demands lead food technology in the development of novel biobased edible food preservatives. Herein, the development and characterization of novel polysaccharide (xanthan gum and kappa-carrageenan)-based nanoemulgels (NGs) enhanced with essential oil derivatives; pure citral (CT); pure carvacrol (CV); and [...] Read more.
Modern bioeconomy and sustainability demands lead food technology in the development of novel biobased edible food preservatives. Herein, the development and characterization of novel polysaccharide (xanthan gum and kappa-carrageenan)-based nanoemulgels (NGs) enhanced with essential oil derivatives; pure citral (CT); pure carvacrol (CV); and various CT:CV ratios (25:75, 50:50, and 75:25) are presented. The obtained NGs are applied as active edible coatings for extending the shelf life of Protected Designation of Origin (PDO) green table olives of Chalkidiki. The zeta potential demonstrated the high stability of the treatments, while light scattering measurement and scanning electron microscopy images confirmed the <100 nm droplet size. EC50 indicated high antioxidant activity for all the tested samples. The fractional inhibitory concentration (FIC) confirmed the synergistic effect of NG with a CT:CV ratio at 50:50 against Staphylococcus aureus and at CT:CV ratios 25:75 and 75:25 against E. coli O157:H7. NG coatings with CT:CV ratios at 50:50 and at 25:75 effectively controlled the weight loss at 0.5%, maintained stable pH levels, and preserved the visual quality of green olives on day 21. The synergistic effect between CT and CV was confirmed as they reduced the spoilage microorganisms of yeasts and molds by 2-log [CFU/g] compared to the control and almost 1 log [CFU/g] difference from pure CT and CV-based NGs without affecting the growth of beneficial lactic acid bacteria crucial for fermentation. NGs with CT:CV ratios at 50:50 and at 25:75 demonstrated superior effectiveness in preventing discoloration and maintaining the main sensory attributes. Overall, shelf life extension was achieved in 21 compared to only 7 of the uncoated ones. Finally, this study demonstrates the potential of polysaccharide-based NGs in mixtures of CT and CV for the shelf life extension of fermented food products. Full article
(This article belongs to the Special Issue Design, Fabrication, and Applications of Food Composite Gels)
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<p>Dynamic light scattering data and SEM images of the materials NGCT, NGCV, NGCTCV_50/50, NGCTCV_25/75, and NGCTCV_75/25.</p>
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<p>Time-killing assay of NGs against <span class="html-italic">S aureus</span> (<b>left</b>) and <span class="html-italic">E coli</span> (<b>right</b>).</p>
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<p>Evaluation of yeast and mold bacteria populations on olives during the 21-day storage period.</p>
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<p>LAB growth of olives during 21-day storage period.</p>
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<p>Visible effect of NGs on moisture control compared to the uncoated sample on Days 7, 14, and 21.</p>
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<p>Schematic presentation of the NG preparation steps.</p>
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17 pages, 1127 KiB  
Article
Effect of Adding Winemaking By-Product on the Characteristics of Petit Suisse Cheese Made with A2A2 Milk and Probiotic
by Cláudia Moreira Santa Catharina Weis, Márcia Miss Gomes, Bárbara Geremia Vicenzi, Giovanna Alexandre Fabiano, Jean de Oliveira Lopes, Patrícia Daniele da Silva dos Santos, Luciano Tormen, Oscar Oliveira Santos, Rosangela Maria Neves Bezerra, Adriane Elisabete Costa Antunes, Larissa Canhadas Bertan, Giselle Nobre Costa and Ricardo Key Yamazaki
Fermentation 2024, 10(11), 570; https://doi.org/10.3390/fermentation10110570 - 8 Nov 2024
Viewed by 501
Abstract
By-products generated in the winemaking industry contain compounds with health-promoting properties, which can be reintroduced into the food production chain. This study evaluated the use of a by-product from the industrial processing of grapes as an ingredient in the manufacture of Petit Suisse [...] Read more.
By-products generated in the winemaking industry contain compounds with health-promoting properties, which can be reintroduced into the food production chain. This study evaluated the use of a by-product from the industrial processing of grapes as an ingredient in the manufacture of Petit Suisse cheese, made with A2A2 milk and the addition of the probiotic Bifidobacterium animalis subsp. lactis HN019. Two Petit Suisse formulations were made in three independent batches: a control formulation without the addition of the by-product (F0) and a formulation containing 10% of the by-product (F1). The proximate composition of the cheeses was characterized on the first day after manufacturing them. The addition of the by-product led to an increase in ash, lipids, and carbohydrates and a reduction in moisture and protein contents. The physicochemical characterization and the texture profile analysis showed no changes throughout the product’s shelf life. The probiotic counts remained abundant (~eight log CFU/g) in both formulations with no changes seen throughout the shelf life period. Scanning electron microscopy images showed the added bacteria had typical structures. No differences were observed in the fatty acid profiles of the formulations, and both exhibited a total of 18 fatty acids, including saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), and polyunsaturated fatty acids (PUFAs). Additionally, the by-product conferred antioxidant activity to the F1 formulation. The addition of the by-product in fresh cheese may be an interesting approach in regards to the processing technology used, its microbiological safety, and its nutritional value. The use of A2A2 milk and a probiotic culture thus enhanced the Petit Suisse cheese, resulting in a healthier product. Full article
(This article belongs to the Special Issue Food Wastes: Feedstock for Value-Added Products: 5th Edition)
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<p>Manufacture of Petit Suisse.</p>
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<p>Scanning electron micrographs of the products. (<b>A</b>) F0 at 8000× magnification; (<b>B</b>) = F0 at 15,000× magnification; (<b>C</b>) = F1 at 8000× magnification; (<b>D</b>) = F1 at 15,000× magnification.</p>
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27 pages, 4071 KiB  
Review
Advances in Emerging Non-Destructive Technologies for Detecting Raw Egg Freshness: A Comprehensive Review
by Elsayed M. Atwa, Shaomin Xu, Ahmed K. Rashwan, Asem M. Abdelshafy, Gamal ElMasry, Salim Al-Rejaie, Haixiang Xu, Hongjian Lin and Jinming Pan
Foods 2024, 13(22), 3563; https://doi.org/10.3390/foods13223563 - 7 Nov 2024
Viewed by 643
Abstract
Eggs are a rich food source of proteins, fats, vitamins, minerals, and other nutrients. However, the egg industry faces some challenges such as microbial invasion due to environmental factors, leading to damage and reduced usability. Therefore, detecting the freshness of raw eggs using [...] Read more.
Eggs are a rich food source of proteins, fats, vitamins, minerals, and other nutrients. However, the egg industry faces some challenges such as microbial invasion due to environmental factors, leading to damage and reduced usability. Therefore, detecting the freshness of raw eggs using various technologies, including traditional and non-destructive methods, can overcome these challenges. As the traditional methods of assessing egg freshness are often subjective and time-consuming, modern non-destructive technologies, including near-infrared (NIR) spectroscopy, Raman spectroscopy, fluorescence spectroscopy, computer vision (color imaging), hyperspectral imaging, electronic noses, and nuclear magnetic resonance, have offered objective and rapid results to address these limitations. The current review summarizes and discusses the recent advances and developments in applying non-destructive technologies for detecting raw egg freshness. Some of these technologies such as NIR spectroscopy, computer vision, and hyperspectral imaging have achieved an accuracy of more than 96% in detecting egg freshness. Therefore, this review provides an overview of the current trends in the state-of-the-art non-destructive technologies recently utilized in detecting the freshness of raw eggs. This review can contribute significantly to the field of emerging technologies in this research track and pique the interests of both food scientists and industry professionals. Full article
(This article belongs to the Section Food Engineering and Technology)
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<p>The basic composition of edible parts of an egg [<a href="#B24-foods-13-03563" class="html-bibr">24</a>].</p>
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<p>A scheme for measuring egg quality parameters of the thick egg white [<a href="#B59-foods-13-03563" class="html-bibr">59</a>].</p>
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<p>Schematic diagram of NIR spectroscopy components for non-destructive detection of egg quality.</p>
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<p>Schematic diagram of Raman spectral analysis for non-destructive detection of external and internal parameters of egg.</p>
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<p>Block scheme of dielectric spectroscopy [<a href="#B22-foods-13-03563" class="html-bibr">22</a>].</p>
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<p>Direct measurement of the fluorescence spectra of egg samples with a laboratory fluorescence acquisition device [<a href="#B119-foods-13-03563" class="html-bibr">119</a>].</p>
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<p>Egg image acquiring system based on machine vision. Source: the authors in the study by Guanjun et al. [<a href="#B85-foods-13-03563" class="html-bibr">85</a>].</p>
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<p>Schematic diagram of hyperspectral imaging technology as non-destructive testing of egg quality.</p>
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22 pages, 46624 KiB  
Article
Autonomous Extraction Technology for Aquaculture Ponds in Complex Geological Environments Based on Multispectral Feature Fusion of Medium-Resolution Remote Sensing Imagery
by Zunxun Liang, Fangxiong Wang, Jianfeng Zhu, Peng Li, Fuding Xie and Yifei Zhao
Remote Sens. 2024, 16(22), 4130; https://doi.org/10.3390/rs16224130 - 5 Nov 2024
Viewed by 481
Abstract
Coastal aquaculture plays a crucial role in global food security and the economic development of coastal regions, but it also causes environmental degradation in coastal ecosystems. Therefore, the automation, accurate extraction, and monitoring of coastal aquaculture areas are crucial for the scientific management [...] Read more.
Coastal aquaculture plays a crucial role in global food security and the economic development of coastal regions, but it also causes environmental degradation in coastal ecosystems. Therefore, the automation, accurate extraction, and monitoring of coastal aquaculture areas are crucial for the scientific management of coastal ecological zones. This study proposes a novel deep learning- and attention-based median adaptive fusion U-Net (MAFU-Net) procedure aimed at precisely extracting individually separable aquaculture ponds (ISAPs) from medium-resolution remote sensing imagery. Initially, this study analyzes the spectral differences between aquaculture ponds and interfering objects such as saltwater fields in four typical aquaculture areas along the coast of Liaoning Province, China. It innovatively introduces a difference index for saltwater field aquaculture zones (DIAS) and integrates this index as a new band into remote sensing imagery to increase the expressiveness of features. A median augmented adaptive fusion module (MEA-FM), which adaptively selects channel receptive fields at various scales, integrates the information between channels, and captures multiscale spatial information to achieve improved extraction accuracy, is subsequently designed. Experimental and comparative results reveal that the proposed MAFU-Net method achieves an F1 score of 90.67% and an intersection over union (IoU) of 83.93% on the CHN-LN4-ISAPS-9 dataset, outperforming advanced methods such as U-Net, DeepLabV3+, SegNet, PSPNet, SKNet, UPS-Net, and SegFormer. This study’s results provide accurate data support for the scientific management of aquaculture areas, and the proposed MAFU-Net method provides an effective method for semantic segmentation tasks based on medium-resolution remote sensing images. Full article
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<p>Study area (A represents the coastal aquaculture area of Qingduizi Bay, Zhuanghe City, Liaoning Province; B represents the coastal aquaculture area north of Maya Island, Pulandian District, Dalian City; C represents the coastal aquaculture area of Yingkou City, east of Liaodong Bay; and D represents the Calabash Island Changshan Temple Bay coastal aquaculture area).</p>
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<p>True colour images of the Yingkou area in January, April, July, and October.</p>
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<p>Spectral analysis charts of the Yingkou area in January, April, July, and October (the green line in <a href="#remotesensing-16-04130-f003" class="html-fig">Figure 3</a> represents the spectral values of the aquaculture ponds across the 13 bands, the red line represents the spectral values of the saltwater fields across the 13 bands, and the orange line represents the spectral values of the embankments across the 13 bands).</p>
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<p>Extraction results for saltwater fields using DIAS with different thresholds.</p>
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<p>Adaptive attention U-Net (MAFU-Net).</p>
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<p>Median-enhanced adaptive fusion module (MEA-FM).</p>
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<p>Visual comparison results of different network models on the CHN-LN4-ISAPs-9 dataset are presented. Among them, (<b>a</b>–<b>h</b>) depict various areas, with the first two columns showing the dataset images and the labeled images, while the subsequent eight columns display the extraction results from different models for each scene. (The black areas represent backgrounds, the white areas represent aquaculture areas, the red elliptical areas indicate misidentified water body regions, the yellow rectangular areas indicate misidentified saltwater fields and other land feature regions, the blue rectangular areas indicate misidentified fallow aquaculture ponds, the orange areas indicate omissions, and the green areas indicate edge adhesion).</p>
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<p>Locations of the verification areas (a represents the Zhangxia Bay coastal aquaculture area in Dalian, Liaoning Province; b represents the Pulandian Bay coastal aquaculture area in Dalian, Liaoning Province; c represents the Taiping Bay coastal aquaculture area in Dalian, Liaoning Province; d represents the southern coastal aquaculture area in Jinzhou, Liaoning Province).</p>
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15 pages, 2351 KiB  
Article
Pesticide Residue Coverage Estimation on Citrus Leaf Using Image Analysis Assisted by Machine Learning
by Adarsh Basavaraju, Edwin Davidson, Giulio Diracca, Chen Chen and Swadeshmukul Santra
Appl. Sci. 2024, 14(22), 10087; https://doi.org/10.3390/app142210087 - 5 Nov 2024
Viewed by 463
Abstract
Globally, the agricultural industry has benefited from using pesticides to minimize crop losses. Nevertheless, the indiscriminate overuse of pesticides has led to significant risks associated with a detrimental impact on the environment and human health. Therefore, emerging concerns of pesticide residue found in [...] Read more.
Globally, the agricultural industry has benefited from using pesticides to minimize crop losses. Nevertheless, the indiscriminate overuse of pesticides has led to significant risks associated with a detrimental impact on the environment and human health. Therefore, emerging concerns of pesticide residue found in crops, food, and livestock are a pressing issue. To address the above challenges, there have been many efforts made towards implementing machine learning to enable precision agricultural practices to reduce pesticide overuse. As of today, there are no guiding digital tools available for citrus growers to provide pesticide residue leaf coverage analysis after foliar applications. Herein, we are the first to report software assisted by lightweight machine learning (ML) to determine the Kocide 3000 and Oxytetracycline (OTC) residue coverage on citrus leaves based on image data analysis. This tool integrates a foundational Segment Anything Model (SAM) for image preprocessing to isolate the area of interest. In addition, Kocide 3000 and Oxytetracycline (OTC) residue coverage analysis was carried out using a specialized Mask Region-Based Convolutional Neural Network (CNN). This CNN was pre-trained on the MS COCO dataset and fine-tuned by training with acquired datasets in laboratory and field conditions. The developed software demonstrated excellent performance on both pesticides’ accuracy, precision, and recall, and F1 score metrics. In summary, this tool has the potential to assist growers with the decision-making process for controlling pesticide use rate and frequency, minimizing pesticide overuse. Full article
(This article belongs to the Section Agricultural Science and Technology)
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<p>Blueprint of the sample compartment design constructed using Onshape.</p>
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<p>Digital images of a citrus leaf with OTC showing the preprocessing of images with the bounding box region of interest within the red box and the output of one segmented image. Source image (<b>Left</b>) with bounding box highlighting the region of interest, and segmented image (<b>Right</b>). Simulated erosion and deposition results in a higher intensity of photoluminescence towards the center of the leaf (<b>Left</b>) due to inherent curvature. Note the debris displayed outside the segmented area (<b>Right</b>), which can result in false positives, hence justifying the need for segmentation and isolation of the region of interest for training the model.</p>
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<p>Diagram of the proposed convolutional neural network (CNN) structure for pesticide residue image analysis.</p>
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<p>(<b>A</b>) Schematic representation of the pesticide deposition on the tree leaves after foliar spray. (<b>B</b>) Calibration curve of OTC at different concentrations based on green-colored coverage area in the digital images from (<b>D</b>) in relation to the leaf surface. (<b>C</b>) Calibration curve of Kocide 3000 at different copper concentrations based on blue-colored coverage area in the digital images from (<b>E</b>) in relation to the leaf surface. (<b>D</b>) Digital images of citrus leaves under UV light exposure showing the OTC residue coverage area. (<b>E</b>) Digital images of citrus leaves under white light exposure showing the Kocide 3000 residue coverage area.</p>
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<p>Overview of the image preprocessing method developed using a leaf spray with OTC under laboratory conditions. (<b>A</b>) Digital image used as a source after bounding box region of interests is determined.(<b>B</b>) collection of segmentation masks generated with the implemented SAM. SAM generates every possible segmentation mask based on the number of objects it can detect in the image. Only the most prominent masks featuring the desired object in focus are selected, in this case the first three masks generated as ranked by SAM.</p>
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<p>Overview of the data processing by the developed Mask R-CNN using a leaf spray with OTC under laboratory conditions. The left image in the red box represents the mask generated by Mask R-CNN, the middle image in the blue box represents the originally segmented image, and the right image in the yellow box represents the superimposed image by combining the previous images. The mask generated by the Mask R-CNN provides a close estimate to the percentage of pesticide coverage through pixel-wise estimation. The superimposed image (right) conveys an overall showcase of both the area and the deposition patterns displayed by the subject.</p>
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29 pages, 3002 KiB  
Review
Detecting Plant Infections: Prospects for Chlorophyll Fluorescence Imaging
by Alyona Grishina, Oksana Sherstneva, Sergey Mysyagin, Anna Brilkina and Vladimir Vodeneev
Agronomy 2024, 14(11), 2600; https://doi.org/10.3390/agronomy14112600 - 4 Nov 2024
Viewed by 462
Abstract
Phytopathogens are a significant challenge to agriculture and food security. In this regard, methods for the early diagnosis of plant diseases, including optical methods, are being actively developed. This review focuses on one of the optical diagnostic methods, chlorophyll fluorescence (ChlF) imaging. ChlF [...] Read more.
Phytopathogens are a significant challenge to agriculture and food security. In this regard, methods for the early diagnosis of plant diseases, including optical methods, are being actively developed. This review focuses on one of the optical diagnostic methods, chlorophyll fluorescence (ChlF) imaging. ChlF reflects the activity of photosynthetic processes and responds subtly to environmental factors, which makes it an excellent tool for the early detection of stressors, including the detection of pathogens at a pre-symptomatic stage of disease. In this review, we analyze the peculiarities of changes in ChlF parameters depending on the type of pathogen (viral, bacterial, or fungal infection), the terms of disease progression, and its severity. The main mechanisms responsible for the changes in ChlF parameters during the interaction between pathogen and host plant are also summarized. We discuss the advantages and limitations of ChlF imaging in pathogen detection compared to other optical methods and ways to improve the sensitivity of ChlF imaging in the early detection of pathogens. Full article
(This article belongs to the Section Pest and Disease Management)
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<p>A common scheme of registration of chlorophyll fluorescence in pulse-modulated mode. A, A<sup>−</sup>—oxidized and reduced electron acceptor, respectively; AL—actinic light; LHC—light-harvesting complex; ML—measuring light; PSII—photosystem II; SP—saturation pulse.</p>
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<p>Images of PVX-GFP-infected tenth tobacco leaf at various DPIs: PVX-GFP—fluorescent images (λex 460 nm and λem 500–540 nm); RGB—RGB images; Φ<sub>PSII</sub>—Φ<sub>PSII</sub> images taken 60 s after the AL was switched on; NPQ—NPQ images taken 40 s after the AL was switched on; 6 DPI corresponds to the first day of PVX-GFP detection in the studied leaf. The pseudocolor scale is shown at the bottom. Tobacco plants were inoculated into the 4th leaf. Infection of the 10th leaf is a result of systemic spread of the virus. The virus contained GFP fluorescent protein that made it possible to determine its localization with high accuracy. Cited from [<a href="#B53-agronomy-14-02600" class="html-bibr">53</a>].</p>
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<p>An example of Arabidopsis leaf infected with GFP-labeled <span class="html-italic">P. syringae</span> on one side of the leaf (upper side) and with wild-type <span class="html-italic">P. syringae</span> on the other site of the leaf (lower side), both in concentration 1 × 10<sup>7</sup> CFU mL<sup>−1</sup>. An image of an inoculated leaf is shown. The fluorescence signal is detected after 3, 20, and 24 h by exciting with either 450 nm (imaging of GFP-labeled pathogen) (<b>A</b>–<b>C</b>) or 365 nm (<b>D</b>–<b>F</b>) (imaging of phenolic fluorescence). Quantum efficiency of PSII (<b>G</b>–<b>I</b>) and maximum efficiency of PSII (F<sub>v</sub>/F<sub>m</sub>) (<b>J</b>–<b>L</b>) are shown for the same time points. The false color scale is given on the right. Cited from [<a href="#B34-agronomy-14-02600" class="html-bibr">34</a>].</p>
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<p>Chlorophyll fluorescence parameters in wheat cultivar CM42 inoculated with two pathotypes <span class="html-italic">P. striiformis f.</span> sp. <span class="html-italic">Tritici</span> CYR32 and V26. An image of an inoculated leaf is shown. F<sub>v</sub>/F<sub>m</sub>, maximum efficiency of Ф<sub>PSII</sub> photochemistry (<b>A</b>). NPQ, non-photochemical quenching (<b>B</b>). Φ<sub>PSII</sub>, quantum yield of PSII electron transport (<b>C</b>). qP, photochemical quenching (qP = (F<sub>m</sub>’ − F<sub>t</sub>’)/(F<sub>m</sub>’ − F<sub>0</sub>)) (<b>D</b>). Quantitative values (±SD) are shown below each fluorescence images. CK, un-inoculated wheat plants. Values 24–120 hpi represent 24, 48, and 120 h post-inoculation. Cited from [<a href="#B39-agronomy-14-02600" class="html-bibr">39</a>].</p>
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<p>Dynamics of ChlF parameters during the progression of diseases caused by pathogens of different types. Color intensity reflects the degree of impact. The dotted line shows the control values of the parameters. The red dot shows the moment of infection. Curves reflect the patterns of change but not quantitative values. The curves are based on the analysis of the results of works [<a href="#B30-agronomy-14-02600" class="html-bibr">30</a>,<a href="#B31-agronomy-14-02600" class="html-bibr">31</a>,<a href="#B33-agronomy-14-02600" class="html-bibr">33</a>,<a href="#B34-agronomy-14-02600" class="html-bibr">34</a>,<a href="#B36-agronomy-14-02600" class="html-bibr">36</a>,<a href="#B37-agronomy-14-02600" class="html-bibr">37</a>,<a href="#B38-agronomy-14-02600" class="html-bibr">38</a>,<a href="#B40-agronomy-14-02600" class="html-bibr">40</a>,<a href="#B43-agronomy-14-02600" class="html-bibr">43</a>,<a href="#B44-agronomy-14-02600" class="html-bibr">44</a>,<a href="#B45-agronomy-14-02600" class="html-bibr">45</a>,<a href="#B50-agronomy-14-02600" class="html-bibr">50</a>,<a href="#B51-agronomy-14-02600" class="html-bibr">51</a>,<a href="#B53-agronomy-14-02600" class="html-bibr">53</a>,<a href="#B57-agronomy-14-02600" class="html-bibr">57</a>,<a href="#B58-agronomy-14-02600" class="html-bibr">58</a>,<a href="#B59-agronomy-14-02600" class="html-bibr">59</a>,<a href="#B63-agronomy-14-02600" class="html-bibr">63</a>,<a href="#B65-agronomy-14-02600" class="html-bibr">65</a>,<a href="#B66-agronomy-14-02600" class="html-bibr">66</a>,<a href="#B67-agronomy-14-02600" class="html-bibr">67</a>].</p>
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<p>Pathways of influence of pathogens on ChlF parameters.</p>
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<p>Influence of PVX on the time course of the quantum yield of PSII (Φ<sub>PSII</sub>) (n = 25): Φ<sub>PSII</sub> images obtained from PVX-GFP-infected tenth tobacco leaf at different times after actinic light (AL) was switched on. The color scale bars indicate the Φ<sub>PSII</sub> value given in false colors. Cited from [<a href="#B53-agronomy-14-02600" class="html-bibr">53</a>].</p>
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