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Search Results (422)

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Keywords = Sub-Saharan area

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21 pages, 1269 KiB  
Article
Human–Wildlife Conflicts, Household Livelihood Security, and Conservation Support Among Residents Adjacent to the Maasai Mara National Reserve in Kenya
by Mercy Chepkemoi Chepkwony, Gasto Jerome Lyakurwa and Edwin Sabuhoro
Wild 2025, 2(1), 6; https://doi.org/10.3390/wild2010006 - 27 Feb 2025
Viewed by 239
Abstract
In sub-Saharan Africa, protected areas are located in close proximity to communities, leading to frequent negative encounters between humans and wildlife. This is coupled with increased pressure from communities seeking park resources to support their livelihoods. To address this, land use plans have [...] Read more.
In sub-Saharan Africa, protected areas are located in close proximity to communities, leading to frequent negative encounters between humans and wildlife. This is coupled with increased pressure from communities seeking park resources to support their livelihoods. To address this, land use plans have been designed around protected areas. For example, in Kenya, wildlife conservancies have been created out of private and community lands. The aim of these conservancies is to contribute to improved livelihoods, reduce human–wildlife conflicts, and increase support for conservation. However, limited research exists on how the conservancies have achieved their aims among local communities. This study focuses on the Maasai Mara region in Kenya and examines how livelihoods and human–wildlife conflicts influence support for conservation among conservancy members. Data were gathered from 411 households adjacent to the reserve through a survey conducted in June and July 2024. The findings indicate a high incidence of human–wildlife conflict and improved security in health and education only. Despite this, there is high satisfaction with the conservancy model and strong support for conservation. Although these findings highlight the significance of conservancies in mitigating human–wildlife conflict, enhancing livelihoods, and garnering conservation support, more attention is needed for food and financial security. Full article
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<p>A Subset map of the Maasai Mara National Reserve and adjacent conservancies in Narok County, Kenya (Source: KWCA).</p>
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32 pages, 3036 KiB  
Article
Agricultural Productivity of Solar Pump and Water Harvesting Irrigation Technologies and Their Impacts on Smallholder Farmers’ Income and Food Security: Evidence from Ethiopia
by Mebratu Negera, Zeleke Agide Dejen, Dagmawi Melaku, Desalegn Tegegne, Muluken Elias Adamseged and Amare Haileslassie
Sustainability 2025, 17(4), 1486; https://doi.org/10.3390/su17041486 - 11 Feb 2025
Viewed by 952
Abstract
Irrigation plays a crucial role in enhancing food production, increasing land productivity, and improving the livelihoods of smallholder farmers in Sub-Saharan Africa (SSA). Solar pumps and water harvesting ponds have emerged as promising technologies for sustainable agriculture for smallholders in SSA and beyond. [...] Read more.
Irrigation plays a crucial role in enhancing food production, increasing land productivity, and improving the livelihoods of smallholder farmers in Sub-Saharan Africa (SSA). Solar pumps and water harvesting ponds have emerged as promising technologies for sustainable agriculture for smallholders in SSA and beyond. The socio-economic impacts of these systems are less studied in the existing literature. This study examined the agricultural productivity of solar pump and water harvesting irrigation technologies and their impacts on income and food security among smallholder farmers in the Central Rift Valley, Lake Hawassa, and Upper Awash sub-basin areas in Ethiopia. Data were collected from 161 farming households that were selected randomly from woredas where solar pump and water harvesting pond irrigation systems had been implemented. The sample size was determined using the power calculation method. Bio-physical observation and measurements were also conducted at field levels. The benefit–cost ratio (BCR) and net water value (NWV) from the use of solar pump and water harvesting pond irrigations were analyzed to assess the viability of these systems. The household food consumption score (HFCS) and household dietary diversity score (HDDS) were calculated to measure food security, while the revenue from crop production was used to measure crop income. An endogenous switching regression model was applied to address the endogeneity nature of the adoption of the irrigation technologies. The counterfactual analysis, specifically the Average Treatment Effect on the Treated (ATT), was used to evaluate the impacts of the irrigation technologies on income and food security. Results indicate that the ATT of crop income, HFCS, and HDDS are positive and statistically significant, illustrating the role of these irrigation systems in enhancing smallholder farmers’ welfare. Moreover, smallholder farmers’ solar pump irrigation systems were found to be economically viable for few crops, with a BCR greater than 1.0 and an NWV ranging from 0.21 to 1.53 USD/m³. It was also found that bundling agricultural technologies with solar pump irrigation systems leads to enhanced agricultural outputs and welfare. The sustainable adoption and scale-up of these irrigation systems demand addressing technical and financial constraints, as well as input and output market challenges. Full article
(This article belongs to the Section Economic and Business Aspects of Sustainability)
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<p>Location of the study area and six target woredas (districts).</p>
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<p>Sample size determination using the power calculation analysis.</p>
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<p>(<b>a</b>) Typical shallow hand-dug well as a water source, (<b>b</b>) solar panel and elevated tank for water storage.</p>
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<p>Area proportion of crops irrigated by solar pumps in the study area.</p>
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<p>BCR for solar pumped irrigation by financing scheme.</p>
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<p>Agricultural technology bundles and their impacts on BCR and net water value. Note: C = intercropping, P = row planting, R = crop rotation, I = improved seed/seedlings, F = chemical fertilizer, O = organic fertilizer, and A = agrochemicals.</p>
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<p>Overall adequacy of irrigation water by irrigated crops.</p>
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<p>Overall adequacy of irrigation water supply under solar pumps by financing scheme.</p>
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<p>Typical water harvesting ponds: (<b>a</b>) large and unlined, (<b>b</b>) small and lined.</p>
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15 pages, 719 KiB  
Article
Relating Workaholism to Job Stress: Serial Mediating Role of Job Satisfaction and Psychological Capital of Nurses in Angola
by Rosa Lutete Geremias
Nurs. Rep. 2025, 15(2), 43; https://doi.org/10.3390/nursrep15020043 - 27 Jan 2025
Viewed by 796
Abstract
Background/Objectives: Previous studies conducted in sub-Saharan African countries have concentrated on examining the challenges of nursing training and the organizational commitment of healthcare professionals, with little attention paid to exploring the mechanisms that contribute to reducing nurses’ job stress. Consequently, the present [...] Read more.
Background/Objectives: Previous studies conducted in sub-Saharan African countries have concentrated on examining the challenges of nursing training and the organizational commitment of healthcare professionals, with little attention paid to exploring the mechanisms that contribute to reducing nurses’ job stress. Consequently, the present study addresses a significant gap in the literature by offering an overview of the factors contributing to understanding job stress among nurses in Angola. This study aimed to analyze the direct and indirect relationship between workaholism and job stress with job satisfaction and psychological capital mediating this relationship. Methods: Using the quantitative methodology with a cross-sectional design, a questionnaire was administered to 340 nurses (172 men and 168 women). Results: The results confirmed that workaholism is positively related to job stress and that job satisfaction and psychological capital serially mediate the relationship between workaholism and job stress. Conclusions: These findings highlight the importance of fostering job satisfaction and psychological capital by establishing favorable work environments and promoting nurses’ physical and emotional well-being. In addition, these results may encourage healthcare leaders to create well-designed break areas for nurses to take restorative breaks. Full article
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<p>Hypothesized model.</p>
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<p>Final model. ** <span class="html-italic">p</span> &lt; 0.01 and *** <span class="html-italic">p</span> &lt; 0.001.</p>
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16 pages, 1011 KiB  
Review
The Otoacoustic Emissions in the Universal Neonatal Hearing Screening: A Scoping Review Update on the African Data (2004 to 2024)
by Stavros Hatzopoulos, Ludovica Cardinali, Piotr Henryk Skarzynski and Giovanna Zimatore
Children 2025, 12(2), 141; https://doi.org/10.3390/children12020141 - 27 Jan 2025
Viewed by 720
Abstract
Background: The reported data on African universal neonatal hearing screening (UNHS) practices tend to be quite scarce, despite the developments in hearing screening the last two decades. The objective of this systematic review was (a) to identify the most recent (in a 20-year [...] Read more.
Background: The reported data on African universal neonatal hearing screening (UNHS) practices tend to be quite scarce, despite the developments in hearing screening the last two decades. The objective of this systematic review was (a) to identify the most recent (in a 20-year span) literature information about NHS/UNHS programs in Africa and (b) to provide data on the procedures used to assess the population, the intervention policies, and on the estimated prevalence of congenital hearing loss with an emphasis on bilateral hearing loss cases. Methods: Queries were conducted via the PubMed, Scopus, and Google Scholar databases for the time window of 2004–2024. The mesh terms used were “OAE”, “universal neonatal hearing screening”, “congenital hearing loss”, “well babies”, and “Africa”. Only research articles and review papers were considered as good candidates. The standard English language filter was not used, to identify information from non-English-speaking scientific communities and groups. Results: Data from 15 papers were considered, reflecting the neonatal hearing practices of nine African states. No country-wide NHS programs were reported. The various screening realities are implemented within big urban centers, leaving the residents of rural areas unassisted. For the latter, proposals based on tele-medicine protocols have been suggested. The data on HL prevalence are also incomplete, but the available data refer to rates from 3 to 360 subjects per 1000. These data cannot be taken at face value but within the small sample size context in which they were acquired. Regarding the causes of HL, very few data have been reported; consanguinity is the most attributed factor, at least in the Sub-Saharan African states. For the majority of the programs, no data were reported on hearing loss prevalence/incidence or on any strategies to restore hearing. Conclusions: The information on the African neonatal hearing screening are quite scarce, and it is an urgent need to convince audiologists from the African localized programs to publish their hearing screening data. Full article
(This article belongs to the Special Issue Hearing Loss in Children: The Present and a Challenge for Future)
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Graphical abstract
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<p>Flow diagram of literature search, according to PRISMA criteria (<a href="http://www.prisma-statement.org/" target="_blank">http://www.prisma-statement.org/</a>, accessed 30 July 2024), with the steps followed in the manuscript selection procedure. After the application of the selection criteria, the initial 74 manuscripts were reduced to 15. Note: the “wrong outcome” voice implies that certain manuscripts were erroneously selected, for example providing data on onchocerciasis-associated epilepsy (OAE) and not on otoacoustic emissions.</p>
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<p>Political map of Africa showing the countries, in green, where NHS activities have been reported in the literature. These African states are reported alphabetically in <a href="#children-12-00141-t002" class="html-table">Table 2</a>.</p>
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32 pages, 1731 KiB  
Article
Towards Sustainable Municipal Solid Waste Management: An SDG-Based Sustainability Assessment Methodology for Innovations in Sub-Saharan Africa
by Julia Weißert, Kristina Henzler and Shimelis Kebede Kassahun
Waste 2025, 3(1), 6; https://doi.org/10.3390/waste3010006 - 17 Jan 2025
Cited by 1 | Viewed by 1122
Abstract
In sub-Saharan Africa, municipal solid waste management faces significant challenges, including inadequate infrastructure, increasing waste generation, and limited resources, leading to severe environmental and public health issues. Innovations in waste management are essential to address these pressing problems, as they can enhance efficiency, [...] Read more.
In sub-Saharan Africa, municipal solid waste management faces significant challenges, including inadequate infrastructure, increasing waste generation, and limited resources, leading to severe environmental and public health issues. Innovations in waste management are essential to address these pressing problems, as they can enhance efficiency, reduce pollution, and promote sustainable practices while fostering sustainable development. To select sustainable and contextually relevant solutions, it is vital to investigate their potential sustainability impacts based on the Sustainable Development Goals (SDGs) beforehand and to involve local stakeholders in the innovation process. Besides, engaging stakeholders increases community buy-in and fosters collaboration, leading to more effective and sustainable outcomes. This paper develops and applies a sustainability assessment methodology for innovations in municipal solid waste management systems in sub-Saharan Africa, with a case study in Ethiopia. The proposed methodology emphasizes the importance of involving local stakeholders in the SDG-based indicator assessment and offers suggestions for a data collection strategy. The case study on a composting process in Bishoftu Town demonstrates that stakeholder participation in selecting innovations positively influences the outcomes. However, the analysis indicates mixed effects of the innovation in the three sustainability dimensions, highlighting areas for optimization. Consequently, the presented method can support the innovation process of municipal solid waste management systems, fostering sustainable municipal development. Full article
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<p>LCSA methodology for MSWMSs in SSA adapted from Wang et al. [<a href="#B19-waste-03-00006" class="html-bibr">19</a>].</p>
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<p>Iteration of processes needed for the LCSA methodology.</p>
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<p>GWP of baseline and comparison scenario.</p>
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<p>Assessment results.</p>
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<p>Indicator set [<a href="#B19-waste-03-00006" class="html-bibr">19</a>,<a href="#B24-waste-03-00006" class="html-bibr">24</a>,<a href="#B25-waste-03-00006" class="html-bibr">25</a>,<a href="#B28-waste-03-00006" class="html-bibr">28</a>,<a href="#B75-waste-03-00006" class="html-bibr">75</a>,<a href="#B76-waste-03-00006" class="html-bibr">76</a>,<a href="#B77-waste-03-00006" class="html-bibr">77</a>,<a href="#B78-waste-03-00006" class="html-bibr">78</a>,<a href="#B79-waste-03-00006" class="html-bibr">79</a>,<a href="#B80-waste-03-00006" class="html-bibr">80</a>,<a href="#B81-waste-03-00006" class="html-bibr">81</a>].</p>
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57 pages, 5169 KiB  
Systematic Review
Systematic Synthesis of Knowledge Relating to the Hydrological Functioning of Inland Valleys in Sub-Saharan Africa
by Akominon M. Tidjani, Pierre G. Tovihoudji, Pierre B. Irénikatché Akponikpe and Marnik Vanclooster
Water 2025, 17(2), 193; https://doi.org/10.3390/w17020193 - 12 Jan 2025
Viewed by 940
Abstract
The potential of inland valleys to enhance food security and improve agricultural resilience to climate change in Africa is constrained by a limited understanding of their hydrological functioning and inadequate water management. In order to synthesize knowledge on hydrological responses in inland valley [...] Read more.
The potential of inland valleys to enhance food security and improve agricultural resilience to climate change in Africa is constrained by a limited understanding of their hydrological functioning and inadequate water management. In order to synthesize knowledge on hydrological responses in inland valley areas, this work reviewed 275 studies from tropical Sub-Saharan Africa (SSA). Data from the literature search were collected from Scopus™, ScienceDirect™, Web of Science™, Google Scholar™, and doctoral theses repositories such as ZEF, HAL, and Theses.fr, covering studies published from the inception of these databases through 31 May 2023. Our approach involved, firstly, a bibliometric analysis of all papers to gain insights into research trends and interests. Secondly, we performed a quantitative synthesis of results from 66 studies examining stream flows in a set of 79 inland valleys to better understand factors that govern runoff dynamics in these environments. Correlative analyses and clustering methods were applied to identify potential links between runoff and watershed physical parameters. The findings highlight the varied responses of inland valleys over both time and space, influenced by a combination of catchment drivers. The correlation matrices between hydrological indices and physical parameters indicate a strong relationship among runoff and a range of parameters, of which the most significant are rainfall (R2 = 0.77) and soil silt content (R2 = 0.68). Challenges in accurately spatializing information related to potential determining components of the water cycle, such as groundwater dynamics and soil moisture, seem to have limited the exploration of interactions between river flow, soil moisture, and groundwater. Future works should prioritize the development of accurate and user-friendly hydrological models that balance complexity and data availability to enhance the understanding of inland valley behavior at fine scales and consolidate food security in Africa. Full article
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<p>Screening sheet of the document classification and selection procedure (according to PRISMA 2020 flow diagram for new systematic reviews, which included searches of databases and registers only). * Consider, if feasible to do so, reporting the number of records identified from each database or register searched (rather than the total number across all databases/registers). ** If automation tools were used, indicate how many records were excluded by a human and how many were excluded by automation tools.</p>
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<p>Temporal dynamics of inland valley hydrology research in Africa.</p>
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<p>Distribution of studies on hydrological issues in inland valleys areas in Africa. Red numbers are the number of studies per country and blue dots represent experimental sites used for quantitative synthesis. In background, the climate map according the Köppen classsification.</p>
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<p>Network of collaborations between researchers working on the hydrological functioning of inland valleys; ‘*’ refers to the name of the most important collaborator of the cluster.</p>
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<p>Rainfall–runoff relations according to time scale in inland valleys: (<b>a</b>) at event scale, (<b>b</b>) at annual scale, and (<b>c</b>) at multi-year scale. Bsh, Aw, and Cwb, respectively, refer to dry, tropical, and temperate climatic groups according to the Köppen classification.</p>
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<p>Runoff coefficients according to time and climate scales in inland valleys: (<b>a</b>) at event scale, (<b>b</b>) at annual scale, and (<b>c</b>) at multi-year scale. Circles refer to individual observations.</p>
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<p>Correlation matrix between physical parameters and hydrological indices in inland valley areas at annual scale. Acronyms means: area (A), minimum elevation (Emin), total relief (TR), slope of flow axis (Sriver), topographic wetness index (TWI), percentage of upstream drainage area (Udra), mean clay content in catchment surface (Cl-Csf), mean sand content in catchment surface (Sa-Csf), mean silt content in catchment surface (Si-Csf), mean clay content in catchment at a depth of 2 m (Cl-C2m), average of absolute depth of bedrock in catchment (Dbed), annual normalized difference vegetation index (NDVI), tree cover (TC), non-tree vegetation area (NTVeg), non-vegetated area (NVA), annual rainfall (Pa), annual actual evapotranspiration (AET), annual total runoff (Qa), and annual total runoff coefficient (Cra). ** <span class="html-italic">p</span> &lt; 0.05, * <span class="html-italic">p</span> &lt; 0.1.</p>
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<p>Correlation matrix between physical parameters and hydrological indices in inland valleys areas at multi-year scale. Acronyms means: area (A), minimum elevation (Emin), total relief (TR), slope of flow axis (Sriver), topographic wetness index (TWI), percentage of upstream drainage area (Udra), mean clay content in catchment surface (Cl-Csf), mean sand content in catchment surface (Sa-Csf), mean silt content in catchment surface (Si-Csf), mean clay content in catchment at a depth of 2 m (Cl-C2m), average of absolute depth of bedrock in catchment (Dbed), mean annual normalized difference vegetation index (NDVImean), mean tree cover (TCmean), non-tree vegetation area (NTVmean), non-vegetated Area (NVAmean), mean annual rainfall (Pmean), mean annual actual evapotranspiration (AETmean), mean total runoff (Qamean), mean runoff coefficient (Crmean). ** <span class="html-italic">p</span> &lt; 0.05, * <span class="html-italic">p</span> &lt; 0.1.</p>
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<p>Scatter plots of runoff coefficients according to land cover factors in inland valleys at annual scale.</p>
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<p>Annual runoff simulations with model based on physical characteristics of inland valleys (<b>a</b>) at calibration and (<b>b</b>) at validation.</p>
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<p>(<b>a</b>) Comparison between observed and simulated discharge at annual scale in inland valleys, (<b>b</b>) residual analysis of observed vs. simulated runoff according to modeling studies in inland valleys.</p>
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14 pages, 571 KiB  
Article
Using a Hand-Held Icterometer to Screen for Neonatal Jaundice: Validation, Feasibility, and Acceptability of the Bili-RulerTM in Kumasi, Ghana
by Ashura Bakari, Ann V. Wolski, Benjamin Otoo, Rexford Amoah, Emmanuel K. Nakua, Jacob Jacovetty, Elizabeth Kaselitz, Sarah D. Compton and Cheryl A. Moyer
Int. J. Environ. Res. Public Health 2025, 22(1), 96; https://doi.org/10.3390/ijerph22010096 - 12 Jan 2025
Cited by 1 | Viewed by 743
Abstract
Background: Neonatal jaundice (NNJ) remains a leading cause of newborn mortality in much of sub-Saharan Africa. We sought to examine the validity of using a hand-held icterometer as a screening tool to determine which newborns need further assessment. Additionally, we sought to assess [...] Read more.
Background: Neonatal jaundice (NNJ) remains a leading cause of newborn mortality in much of sub-Saharan Africa. We sought to examine the validity of using a hand-held icterometer as a screening tool to determine which newborns need further assessment. Additionally, we sought to assess the feasibility of its use among mothers. Methods: We recruited and trained healthcare workers at one large district hospital in Ghana to use a hand-held icterometer known as the Bili-RulerTM. We recruited mothers of 341 newborns aged 0 to 2 weeks at the same hospital. Mothers watched a standardized training video, after which they blanched the skin of the newborn’s nose and compared it with the yellow shades numbered one to six on the icterometer. Each newborn was also assessed with a transcutaneous bilirubin meter (TCB). Research assistants and health care workers screened the same newborns, recorded their scores separately, and were blinded to each other’s readings. In the second phase of this study, we recruited 100 new mothers to take the Bili-Ruler home with them, instructing them to check their newborns twice daily. We interviewed them 1–2 weeks later to determine the acceptability and feasibility of its use. Results: Out of 341 newborns screened, 20 had elevated TCB indicative of hyperbilirubinemia. Healthcare workers’ Bili-Ruler ratings had a strong and significant correlation with TCB scores, as did the ratings of researchers and mothers. When comparing Bili-Ruler scores against TCB, sensitivity across all three raters was 80% (95% CI 75.6–84.3), specificity ranged from 61.1% (healthcare providers) to 66.7% (researchers), positive predictive value ranged from 11.4% (healthcare providers) to 13.0% (researchers), and negative predictive value was 98.0% or higher across all raters. Area under the ROC curve ranged from 0.71 for healthcare providers to 0.73 for researchers. Mothers AUC was 0.72. In terms of acceptability and feasibility, the Bili-Ruler was widely accepted by the mothers and family. In total, 98% of mothers reported using it, and 90.8% used it 3 or more days in the first week after birth. Moreover, 89.8% used it more than twice per day. Conclusions: A hand-held, low-tech icterometer is an important potential mechanism for improving early jaundice identification in low-resource settings. Further studies using larger sample sizes with a higher prevalence of hyperbilirubinemia are warranted. Full article
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<p>Hand-held Bili-Ruler<sup>TM</sup> for monitoring jaundice; newborn’s nose is lightly blanched with the Bili-Ruler, and blanched skin is viewed through the hole in the center of the color swatch for color comparison. A 3 or higher is indicative of the need for additional screening.</p>
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<p>Area under the ROC curves for each type of rater’s Bili-Ruler score when compared to TCB score.</p>
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16 pages, 1914 KiB  
Article
Co-Infection of Culex tarsalis Mosquitoes with Rift Valley Fever Phlebovirus Strains Results in Efficient Viral Reassortment
by Emma K. Harris, Velmurugan Balaraman, Cassidy C. Keating, Chester McDowell, J. Brian Kimble, Alina De La Mota-Peynado, Erin M. Borland, Barbara Graham, William C. Wilson, Juergen A. Richt, Rebekah C. Kading and Natasha N. Gaudreault
Viruses 2025, 17(1), 88; https://doi.org/10.3390/v17010088 - 11 Jan 2025
Viewed by 1003
Abstract
Rift Valley fever phlebovirus (RVFV) is a zoonotic mosquito-borne pathogen endemic to sub-Saharan Africa and the Arabian Peninsula which causes Rift Valley fever in ruminant livestock and humans. Co-infection with divergent viral strains can produce reassortment among the L, S, and M segments [...] Read more.
Rift Valley fever phlebovirus (RVFV) is a zoonotic mosquito-borne pathogen endemic to sub-Saharan Africa and the Arabian Peninsula which causes Rift Valley fever in ruminant livestock and humans. Co-infection with divergent viral strains can produce reassortment among the L, S, and M segments of the RVFV genome. Reassortment events can produce novel genotypes with altered virulence, transmission dynamics, and/or mosquito host range. This can have severe implications in areas where RVFV is endemic and convolutes our ability to anticipate transmission and circulation in novel geographic regions. Previously, we evaluated the frequency of RVFV reassortment in a susceptible ruminant host and observed low rates of reassortment (0–1.7%). Here, we tested the hypothesis that reassortment occurs predominantly in the mosquito using a highly permissive vector, Culex tarsalis. Cells derived from Cx. tarsalis or adult mosquitoes were co-infected with either two virulent (Kenya-128B-15 and SA01-1322) or a virulent and attenuated (Kenya-128B-15 and MP-12) strain of RVFV. Our results showed approximately 2% of virus genotypes isolated from co-infected Cx. tarsalis-derived cells were reassortant. Co-infected mosquitoes infected via infectious bloodmeal resulted in a higher percentage of reassortant virus (2–60%) isolated from midgut and salivary tissues at 14 days post-infection. The percentage of reassortant genotypes isolated from the midguts of mosquitoes co-infected with Kenya-128B-15 and SA01-1322 was similar to that of mosquitoes co-infected with Kenya-128B-15 and MP-12- strains (60 vs. 47%). However, only 2% of virus isolated from the salivary glands of Kenya-128B-15 and SA01-1322 co-infected mosquitoes represented reassortant genotypes. This was contrasted by 54% reassortment in the salivary glands of mosquitoes co-infected with Kenya-128B-15 and MP-12 strains. Furthermore, we observed preferential inclusion of genomic segments from the three parental strains among the reassorted viruses. Replication curves of select reassorted genotypes were significantly higher in Vero cells but not in Culex—derived cells. These data imply that mosquitoes play a crucial role in the reassortment of RVFV and potentially contribute to driving evolution of the virus. Full article
(This article belongs to the Special Issue Emerging Highlights in the Study of Rift Valley Fever Virus)
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<p>In vitro co-infection of <span class="html-italic">Cx</span>. <span class="html-italic">tarsalis</span> cells (CxTxR2) with two strains of RVFV results primarily in recovery of parental Kenya-128B-15 virus strain with low frequency of RAVs detected. CxTxR2 cells were co-infected at 0.1 MOI with RVFV Kenya-128B-15 and MP-12 (<b>A</b>) or Kenya-128B-15 and SA01-1322 (<b>B</b>). Virus supernatant was collected at 3 days post-infection (dpi) and virus was plaque purified for genotyping analysis to determine segmental composition. Results are based on one independent experiment.</p>
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<p>Rift Valley fever virus co-infection in <span class="html-italic">Cx. tarsalis</span> produces viral genotypes representing parental and reassortant strains across mosquito midgut and salivary gland tissue. Adult female mosquitoes were provided an RVFV infectious bloodmeal containing either Kenya-128B-15 and MP-12 or Kenya-128B-15 and SA01-1322. At 14 dpi, midgut (dark blue) and salivary glands (light blue) were dissected from female mosquitoes (n = 30) and pooled into tissue-specific tubes. The virus was isolated from tissue-specific homogenates and genotyped to determine segmental composition (<b>A</b>). Percent genotyped virus on the <span class="html-italic">y</span>-axis with segmental composition on the <span class="html-italic">x</span>-axis. The number of each genotype detected over the total number of plaques analyzed from the midgut and salivary gland tissues of Kenya-128B-15 and MP-12 (<b>B</b>) Kenya-128B-15 and SA01-1322 (<b>C</b>) co-infected mosquitoes are shown. Data are representative of two independent experiments.</p>
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<p>Analysis of segmental composition in isolated RAV genotypes from infected <span class="html-italic">Cx. tarsalis</span> reveals patterns of reassortment present in the majority or minority of isolated viruses. Raw counts of the L-, M-, or S -segment recovered from <span class="html-italic">Cx. tarsalis</span> midgut and salivary gland tissue were totaled and grouped by co-infection with either Kenya-128B-15 and MP-12 (<b>A</b>) or Kenya-128B-15 and SA01-1322 (<b>B</b>).</p>
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<p>Growth curves of frequently detected reassortant RVFV genotypes isolated from co-infected <span class="html-italic">Cx. tarsalis</span> compared to parental strains reveals comparable or decreased overall growth curves across cell types. Growth kinetics of parental and RAVs (Kenya-128B-15<sub>LS</sub>:MP-12<sub>M</sub> and Kenya-128B-15<sub>S</sub>:MP-12<sub>LM</sub>) in Vero MARU (<b>A</b>) and CxTxR2 (<b>B</b>) cells. An MOI of 0.01 was used for infection and supernatant collected post-infection. The mean growth curve for each reassortant strain was compared to parental using a two-sample <span class="html-italic">t</span>-test. Data with <span class="html-italic">p</span> &lt; 0.05 were considered significant. All data are indicative of two independent experiments.</p>
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<p>Reassortant viruses generated by co-infection between Kenya-128B-15 and SA01-1322 replicated to higher titers than parental strains in Vero MARU cells but not CxTxR2 cells. Growth curves of the parental strains and the two most frequently recovered RAVs isolated from co-infected <span class="html-italic">Cx. tarsalis</span> were analyzed in Vero MARU (<b>A</b>) and CxTxR2 (<b>B</b>) cells. Each viral strain was inoculated into respective cell lines at an MOI of 0.01 and supernatant collected post-infection. The mean growth curve for each reassortant strain was compared to parental using a two-sample <span class="html-italic">t</span>-test. Data with <span class="html-italic">p</span> &lt; 0.05 were considered significant. All data are indicative of two independent experiments.</p>
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34 pages, 2377 KiB  
Systematic Review
The Impact of Onchocerciasis Elimination Measures in Africa: A Systematic Review
by Policarpo Ncogo, Christine Giesen, María Jesús Perteguer, Maria P. Rebollo, Rufino Nguema, Agustín Benito and Zaida Herrador
Trop. Med. Infect. Dis. 2025, 10(1), 7; https://doi.org/10.3390/tropicalmed10010007 - 26 Dec 2024
Viewed by 882
Abstract
Background: Onchocerciasis, or river blindness, is one of the neglected tropical diseases (NTDs) that the WHO has set out to eliminate. To reach this elimination target, a number of challenges must be met, and the essential measures set out in the road map [...] Read more.
Background: Onchocerciasis, or river blindness, is one of the neglected tropical diseases (NTDs) that the WHO has set out to eliminate. To reach this elimination target, a number of challenges must be met, and the essential measures set out in the road map for NTDs 2021–2030 must be implemented. More than 99% of infected people live in 31 countries in sub-Saharan Africa. Our objective was to assess the impact of onchocerciasis interventions in Africa. Methodology: A systematic peer review of the existing literature following the PRISMA guidelines was performed between November 2021 and April 2022. We selected studies on onchocerciasis control measures in Africa since the implementation of the first Onchocerciasis Control Programme (OCP) measures in 1974. All scientific articles indexed in the PubMed, Scopus, Embase, and CENTRAL databases written in Spanish, English, French, German, and Portuguese were considered. The study protocol was registered in the PROSPERO database. Results: A total of 63 articles met the inclusion criteria and were finally selected. Publications were found from 19 out of 31 African endemic countries. The main intervention retained in the different published studies was mass distribution of ivermectin (n = 51). According to our results, 11 African countries have managed to interrupt transmission of onchocerciasis in at least one area in the country; 11 countries have not achieved this goal, while 1 country has managed to eliminate the disease, but it has resurged. Conclusions: Control interventions showed a positive impact on the fight against onchocerciasis, demonstrating that these activities are effective. Nevertheless, they were not sufficient to achieve the proposed goals for a variety of reasons. Therefore, different aspects should be considered in order to fulfil the targets proposed by the WHO to be reached in 2030. Full article
(This article belongs to the Section Neglected and Emerging Tropical Diseases)
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<p>PRISMA flow chart. * Consulted databases and registers were PubMed, Scopus, Embase, and CENTRAL. ** No automation tools were used.</p>
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<p>Published articles by African endemic countries. <b>Above</b>: Countries with studies assessing only CDTI. <b>Middle</b>: Countries assessing only vector control strategies. <b>Below</b>: Countries assessing mixed methods. Countries where no studies were performed are represented in grey.</p>
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<p>Published articles by African endemic countries. <b>Above</b>: Countries with studies assessing only CDTI. <b>Middle</b>: Countries assessing only vector control strategies. <b>Below</b>: Countries assessing mixed methods. Countries where no studies were performed are represented in grey.</p>
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<p>Published articles by African endemic countries. <b>Above</b>: Countries with studies assessing only CDTI. <b>Middle</b>: Countries assessing only vector control strategies. <b>Below</b>: Countries assessing mixed methods. Countries where no studies were performed are represented in grey.</p>
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<p>Intervention outcomes by country. Some studies were performed in certain regions of the countries. However the whole country shape is highlighted for better visibility. Countries where no studies were performed are represented in grey.</p>
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19 pages, 2274 KiB  
Review
Urban Forestry in Sub-Saharan Africa: Challenges, Contributions, and Future Directions for Combating Climate Change and Restoring Forest Landscapes
by Sawaba Ale, Kossi Adjonou, Kossi Novigno Segla, Kossi Komi, Jean-Bosco Benewinde Zoungrana, Coffi Aholou and Kouami Kokou
Sustainability 2025, 17(1), 24; https://doi.org/10.3390/su17010024 - 24 Dec 2024
Viewed by 953
Abstract
Since the 1960s, the countries of sub-Saharan Africa have been affected by an urban crisis due to the demographic explosion in cities and the resulting land pressure. These issues have resulted in the proliferation of spontaneous housing areas, which call the future of [...] Read more.
Since the 1960s, the countries of sub-Saharan Africa have been affected by an urban crisis due to the demographic explosion in cities and the resulting land pressure. These issues have resulted in the proliferation of spontaneous housing areas, which call the future of urban vegetation and its degree of inclusion in city planning into question. To analyze the current scientific knowledge on the development of urban landscapes, particularly in sub-Saharan Africa, a literature review on urban forestry covering the period of 1998–2022 was carried out in order to better guide future research works towards a greater consideration of urban forestry and its contribution to combating the adverse effects of climate change. Out of the 110 scientific papers selected, 50 were considered relevant because of their direct link with urban forestry. Based on the analysis of these papers, a classification of the articles according to five themes was carried out. These themes are the management of green spaces in urban areas, the functions of trees in an urban environment, the development of urban forestry, the characterization of urban tree flora, and the financing of urban forestry. Thus, the “function of trees in an urban environment and the characterization of urban tree flora” are the most addressed issues, being raised in 42% and 28% of articles, respectively. “Financing urban forestry” is practically not addressed (2%). The analysis of the 50 selected articles reveals a significant geographic concentration of urban forestry research. Countries such as South Africa, Kenya, and Ghana are more represented in the literature, while vast regions in Central and Western Africa (e.g., the Democratic Republic of Congo, Chad, and Niger) are the object of minimal or no documented research with regard to urban forestry. Future research should address these underrepresented themes, as they are crucial for the long-term sustainability of urban forestry projects. Particularly, research on financing mechanisms is essential to unlocking sustainable funding sources for urban forestry, a critical barrier to its expansion. Full article
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<p>Map of Regions of Sub-Saharan Africa. Source: USDA, Economic Research Service.</p>
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<p>The synthesis of selection processes used to select the articles included in the analysis.</p>
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<p>Themes addressed in urban forestry research in sub-Saharan Africa.</p>
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<p>Topics covered in the literature by country. Legend: Management of green spaces in urban areas (GEVMU), Functions of trees in urban areas (FAMU), Development of urban forestry (DFU), Characterization of urban tree flora (CFAU) et Financing urban forestry (FFU).</p>
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<p>Articles published on urban forestry by country.</p>
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<p>Urban forestry articles by year.</p>
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22 pages, 13685 KiB  
Article
An Interdisciplinary Analysis of the Late Islamic Cemeteries Within the Cathedral (15th–18th Century CE) and the Ruins of Adulis (Mid-19th–Early 20th Century CE), Massawa, Eritrea: Funerary Architecture, Funerary Rituals, Burial Rites, and Bioarcheological Data Identifying Late Islamic Graves in Central Eastern Eritrea
by Omar Larentis, Nelly Cattaneo, Paolo Lampugnani, Susanna Bortolotto, Emanuele Zappa, Andrea Gregorini, Yotam Gezae, Tsegai Medin, Ilaria Gorini and Serena Massa
Heritage 2025, 8(1), 1; https://doi.org/10.3390/heritage8010001 - 24 Dec 2024
Viewed by 748
Abstract
Systematic studies on Late Islamic cemeteries that integrate architectural, ritual, and biological aspects remain relatively rare, particularly in Islamic countries or regions with an active Muslim presence. Typically, available research focuses more on epigraphic and artistic features. Since 2018, excavations at the Cathedral [...] Read more.
Systematic studies on Late Islamic cemeteries that integrate architectural, ritual, and biological aspects remain relatively rare, particularly in Islamic countries or regions with an active Muslim presence. Typically, available research focuses more on epigraphic and artistic features. Since 2018, excavations at the Cathedral and surveys in the ruins of the city of Adulis (Massawa, Eritrea) have uncovered 326 Muslim graves: six dating to the 15th and 18th centuries CE and 320 attributed to the mid-19th to the early 20th century CE. These discoveries have enabled, for the first time in Eritrea, a detailed scientific study of the biological characteristics of the human remains, the funerary architecture, and the burial rites of Muslim communities from the 15th to the early 20th century. The skeletal remains were analyzed through taphonomic and anthropological studies. The graves were categorized into various typologies based on their structural features, while evidence of funerary rituals was documented as indicative elements of the burial practices adopted. These findings were further enriched by ethnographic sources and collaborative work with local communities, who acted as custodians of the historical memory and traditions of the region. The results provide insights into a historical period of Eritrea that is otherwise poorly documented. More broadly, they contribute to the understanding of the history of the Horn of Africa, a region with limited data from funerary and archaeological contexts. The Muslim cemeteries of Adulis represent the most extensively investigated Muslim burial site in recent years, providing critical insights into the historical Muslim presence in the region. These findings also allow researchers to hypothesize the succession of various communities that chose Adulis as a central location for interring their deceased, reflecting its enduring significance as a focal point for cultural and ritual practices across different periods. Full article
(This article belongs to the Section Archaeological Heritage)
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<p>(<b>a</b>) Location of the archaeological site of Adulis (Longitude: 39.6602053, Latitude: 15.262725), Zula Bay, situated between the villages of Afta and Zula in the Foro district, south of Massawa. (<b>b</b>) Excavations in the city have uncovered numerous secular buildings, such as the “palace” excavated by Richard Sundström and religious structures like the Northern Urban Church in Sector 2, the so-called “Eastern Church” in Sector 4, the church known as “The English Church”, now referred to as the Cathedral, and an elitarian building close to the Cathedral in Sectors 3–6. Graphic elaboration by Nelly Cattaneo.</p>
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<p>Orthoimage of the Cathedral after the 2023 excavation campaign. Foundations of structures, outlined with lines, rendered for better clarity. Excavated tombs (SU 6039, 6041, 6116b, 6124) are highlighted in blue, with probable tombs (SU 6126, 6127) in light blue with perimeter markings.</p>
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<p>(<b>a</b>) SU 6116b during excavation. (<b>b</b>) SU 6124 in the last part of the excavation. (<b>c</b>) DEM of area SU 6116b during excavation obtained with photogrammetry. (<b>d</b>) DEM of area SU 6124 in the last part of the excavation obtained with photogrammetry. Dashed lines connect the same burial.</p>
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<p>Textured 3D models obtained with photogrammetry of (<b>a</b>,<b>c</b>) SU 6116b during excavation and (<b>b</b>,<b>d</b>) SU 6124 in the last part of the excavation. Dashed lines connect the same burial.</p>
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<p>Tomb SU 6124. (<b>a</b>–<b>e</b>) The various excavation phases, presented in chronological order, highlight the tomb within the nave, delineated by two schist slabs vertically embedded in the ground marking the pit boundaries. (A) A platform, just a few centimeters above the cut, was formed by flat-lying schist slabs placed horizontally on the ground ((<b>a</b>), white rectangle). (<b>e</b>,<b>f</b>) Adjacent to the southern vertical slab, a thick lens of soil rich in charcoal was uncovered ((<b>e</b>), white rectangle). Arrows sign the North.</p>
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<p>(<b>a</b>) Southern schist slab of tomb SU 6124, showing an area rich in carbonaceous material with a darker color (indicated by a white rectangle) and the location where the cowry shell was found (marked with a white circle). (<b>b</b>) Top and bottom views of the cowry shell, displaying its perforation. (<b>c</b>,<b>d</b>) Local ceramic fragments recovered from the slate slab platform near the tomb.</p>
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<p>(<b>a</b>) During the survey, a Zula village elder demonstrated the construction technique of Muslim tombs. In this example, two lines drawn in the sand represent the trench and the lateral chamber carved at the trench’s base to house the deceased. (<b>b</b>,<b>c</b>) For each tomb, data such as size, funerary architecture, and the presence of ritual elements were recorded.</p>
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<p>(<b>a</b>) Tomb of a prominent figure within the Zula community characterized by elaborate architecture. (<b>b</b>–<b>f</b>) Nearby graves, especially those close to more complex structures, exhibit prestigious funerary architecture. These are typically rectangular or ellipsoidal in shape and rise significantly above ground level, surrounded by perimeter walls made of flat-laid shale slabs.</p>
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<p>(<b>a</b>) Circular tomb constructed with roughly hewn basalt blocks. (<b>b</b>,<b>c</b>) Circular tomb surrounded by squared basalt blocks. (<b>d</b>) Tomb identifiable by the white quartz pebbles. (<b>e</b>) Probable female tomb demarcated by squared basalt blocks. (<b>f</b>) Tomb damaged by erosion from the Haddas River.</p>
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<p>The tomb point-shape file on the satellite imagery. In red, Bet Khalifa’s Clan cemetery; in orange, Scheik Mahmoud’s Clan cemetery; and in yellow, the other graves. North is at the top of the figure.</p>
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16 pages, 1226 KiB  
Article
House Mice in the Atlantic Region: Genetic Signals of Their Human Transport
by Sofia I. Gabriel, Jonathan J. Hughes, Jeremy S. Herman, John F. Baines, Mabel D. Giménez, Melissa M. Gray, Emilie A. Hardouin, Bret A. Payseur, Peter G. Ryan, Alejandro Sánchez-Chardi, Rainer G. Ulrich, Maria da Luz Mathias and Jeremy B. Searle
Genes 2024, 15(12), 1645; https://doi.org/10.3390/genes15121645 - 21 Dec 2024
Viewed by 869
Abstract
Background/Objectives: The colonization history of house mice reflects the maritime history of humans that passively transported them worldwide. We investigated western house mouse colonization in the Atlantic region through studies of mitochondrial D-loop DNA sequences from modern specimens. Methods: We assembled a dataset [...] Read more.
Background/Objectives: The colonization history of house mice reflects the maritime history of humans that passively transported them worldwide. We investigated western house mouse colonization in the Atlantic region through studies of mitochondrial D-loop DNA sequences from modern specimens. Methods: We assembled a dataset of 758 haplotypes derived from 2765 mice from 47 countries/oceanic archipelagos (a combination of new and published data). Our maximum likelihood phylogeny recovered five previously identified clades, and we used the haplotype affinities within the phylogeny to infer house mouse colonization history, employing statistical tests and indices. From human history, we predefined four European source areas for mice in the Atlantic region (Northern Europe excluding Scandinavia, Southern Europe, Scandinavia, and Macaronesia) and we investigated the colonization from these source areas to different geographic areas in the Atlantic region. Results: Our inferences suggest mouse colonization of Scandinavia itself from Northern Europe, and Macaronesia from both Southern Europe and Scandinavia/Germany (the latter likely representing the transport of mice by Vikings). Mice on North Atlantic islands apparently derive primarily from Scandinavia, while for South Atlantic islands, North America, and Sub-Saharan Africa, the clearest source is Northern Europe, although mice on South Atlantic islands also had genetic inputs from Macaronesia and Southern Europe (for Tristan da Cunha). Macaronesia was a stopover for Atlantic voyages, creating an opportunity for mouse infestation. Mice in Latin America also apparently had multiple colonization sources, with a strong Southern European signal but also input from Northern Europe and/or Macaronesia. Conclusions: D-loop sequences help discern the broad-scale colonization history of house mice and new perspectives on human history. Full article
(This article belongs to the Collection Feature Papers in ‘Animal Genetics and Genomics’)
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<p>Summary phylogenetic tree for all house mouse haplotypes under consideration, highlighting the particular source areas for Atlantic colonization associated with each of the five previously named clades (B–F) found in the region. The outgroups and the haplotypes that could not be attributed with confidence to a previously named clade had their branches colored black. The naming of the geographic areas follows the convention in this paper. See <a href="#app1-genes-15-01645" class="html-app">Figures S1 and S2</a> for the full tree (including branch support) and further explanation. The phylogeny is based on our new sequences (<a href="#app1-genes-15-01645" class="html-app">Table S1</a>) and previously published sequences (<a href="#app1-genes-15-01645" class="html-app">Text S2</a>). Particular sequences of importance for interpretation (see <a href="#sec3dot2-genes-15-01645" class="html-sec">Section 3.2</a> below) are presented in <a href="#app1-genes-15-01645" class="html-app">Table S3</a> (with subsidiary information in <a href="#app1-genes-15-01645" class="html-app">Table S2</a>).</p>
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<p>Inferred colonization history of house mice in the Atlantic region from the four defined source areas (Northern Europe, Southern Europe, Scandinavia, Macaronesia) to the five defined colonized areas (North Atlantic, North America, Latin America, South Atlantic, Sub-Saharan Africa). Solid arrows indicate the main routes of colonization. For Latin America, there is a major signal of mouse colonization from Southern Europe but also data suggesting at least partial derivation from Macaronesia and/or Northern Europe (shown with dashed arrows). For the South Atlantic islands, the clade analysis indicates the pre-eminence of Northern Europe in mouse colonization, but multi-location haplotypes support the involvement of Macaronesia and Southern Europe as well (dotted arrows). The most indicative clades present in the source and colonized areas for each of these linkages are shown within circles. There are insufficient data to infer the colonization history of the Caribbean by house mice. The coloring used here does not relate to the coloring in the phylogenetic trees. AP: Azores, CV: Cabo Verde, FK: Falkland (Malvinas) Islands, FO: Faroe, GL: Greenland, GP: Guadeloupe, GS: South Georgia, IC: Canary Islands, IS: Iceland, MQ: Martinique, TA: Tristan da Cunha, XG: Gough Island, XM: Madeira, XP: Marion Island.</p>
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23 pages, 2131 KiB  
Article
Exploring the Feasibility of Sorghum Farming in South Africa Using Garrett’s Ranking Technique
by Zamaswazi Nkosi, Nyankomo Marwa and Olawale Olufemi Akinrinde
Agriculture 2024, 14(12), 2348; https://doi.org/10.3390/agriculture14122348 - 20 Dec 2024
Viewed by 836
Abstract
The potential for sorghum to be the driver of economic development in Sub-Saharan economies, including South Africa, is enormous. However, there has been a notable decline in the production, use, and consumption of sorghum due to the changes in preferences and lifestyle of [...] Read more.
The potential for sorghum to be the driver of economic development in Sub-Saharan economies, including South Africa, is enormous. However, there has been a notable decline in the production, use, and consumption of sorghum due to the changes in preferences and lifestyle of its consumers in Southern Africa. While existing and extant studies have studied the benefits and impacts of sorghum on the African economy, there is an inadequate understanding of the feasibility of sorghum farming in South Africa using Garrett’s ranking technique. As a result, this study explores the feasibility of sorghum farming in the Nyoni area of KwaZulu Natal, South Africa. Hence, a survey was conducted with a sample size of 150 respondents that were purposively selected. An interview schedule was also used to collect primary data, and the data were analyzed using Garrett’s ranking technique. The results of this study indicated that edaphic factors, climatic factors, volatile markets, information transfers, education, drought resistance variety, access to credit, and technological advancement were critical in making sorghum farming feasible in the Nyoni area of KwaZulu Natal. Therefore, this study recommends that there is an overarching need to explore the feasibility of sorghum farming, starting at a smaller scale until a more stable state of marketability and potential profitability has been established. Full article
(This article belongs to the Section Agricultural Economics, Policies and Rural Management)
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<p>Sorghum production areas in South Africa [<a href="#B25-agriculture-14-02348" class="html-bibr">25</a>].</p>
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<p>Sorghum yields in selected countries, including South Africa. Source: SAGov, Agriculture, Land Reform, and Rural Development.</p>
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<p>Gender of the respondents.</p>
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<p>Job of the respondents.</p>
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<p>Age of the respondents.</p>
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<p>Work/farming experience of the respondents.</p>
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30 pages, 1608 KiB  
Article
Does Adopting the Bean Technology Bundle Enhance Food Security and Resilience for Smallholder Farmers in Ethiopia?
by Enid Katungi, Endeshaw Habte, Paul Aseete and Jean Claude Rubyogo
Sustainability 2024, 16(24), 10914; https://doi.org/10.3390/su162410914 - 12 Dec 2024
Cited by 1 | Viewed by 997
Abstract
The analysis of the differential impacts of multiple improved technologies has largely accounted for selective adoption, considering either the full application of a bundle or its individual components. The impacts of adopting agricultural technology bundles on household welfare are less understood when considering [...] Read more.
The analysis of the differential impacts of multiple improved technologies has largely accounted for selective adoption, considering either the full application of a bundle or its individual components. The impacts of adopting agricultural technology bundles on household welfare are less understood when considering a partial adoption of either the entire bundle or its individual components on a portion of crop area. We assess simultaneous adoption and the impacts of multiple improved technologies promoted as a bundle and recommended for legume intensification systems for smallholder farmers in Ethiopia. We use DNA fingerprinting data to precisely identify our key treatment—“adoption of improved bean varieties”—in this study. Using an endogenous multivariate treatment effects model, we found significant positive impacts of adopting bundled interventions on agricultural incomes and household food security but vulnerability to food insecurity persists for many households. We find that growing improved varieties with fertilizers increased household agricultural revenue, allowing for more legume consumption and enhancing their likelihood of achieving adequate food consumption and food security outcomes; however, the vulnerability to food insecurity of the adopters remains high due to pre-existing resource degradation issues. Given the similarity in production contexts in Sub-Saharan Africa, our results provide perspective for similar development interventions. We use the results of our analysis to discuss potential policy implications and programs to support technological intensification among smallholder farmers. Full article
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<p>Distribution of outcome variables by adoption category: (<b>a</b>) per capita food expenditure; (<b>b</b>) consumption score; (<b>c</b>) quantity of food in storage at the end of the season; (<b>d</b>) agricultural income; (<b>e</b>) share of household budget spent on food; (<b>f</b>) household food insecurity assessment score.</p>
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<p>Distribution of outcome variables by adoption category: (<b>a</b>) per capita food expenditure; (<b>b</b>) consumption score; (<b>c</b>) quantity of food in storage at the end of the season; (<b>d</b>) agricultural income; (<b>e</b>) share of household budget spent on food; (<b>f</b>) household food insecurity assessment score.</p>
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14 pages, 2867 KiB  
Article
Non-Invasive Malaria Detection in Sub-Saharan Africa Using a DNA-Based Sensor System
by Trine Juul-Kristensen, Celine Thiesen, Line Wulff Haurum, Josephine Geertsen Keller, Romeo Wenceslas Lendamba, Rella Zoleko Manego, Madeleine Eunice Betouke Ongwe, Birgitta Ruth Knudsen, Eduardo Pareja, Eduardo Pareja-Tobes, Rodrigo Labouriau, Ghyslain Mombo-Ngoma and Cinzia Tesauro
Sensors 2024, 24(24), 7947; https://doi.org/10.3390/s24247947 - 12 Dec 2024
Viewed by 1073
Abstract
Malaria poses a serious global health problem, with half the world population being at risk. Regular screening is crucial for breaking the transmission cycle and combatting the disease spreading. However, current diagnostic tools relying on blood samples face challenges in many malaria-epidemic areas. [...] Read more.
Malaria poses a serious global health problem, with half the world population being at risk. Regular screening is crucial for breaking the transmission cycle and combatting the disease spreading. However, current diagnostic tools relying on blood samples face challenges in many malaria-epidemic areas. In the present study, we demonstrate the detection of the malaria-causing Plasmodium parasite in non-invasive saliva samples (N = 61) from infected individuals by combining a DNA-based Rolling-circle-Enhanced-Enzyme-Activity-Detection (REEAD) sensor system with a chemiluminescence readout that could be detected with an in-house-developed affordable and battery-powered portable reader. We successfully transferred the technology to sub-Saharan Africa, where the malaria burden is high, and demonstrated a proof of concept in a small study (N = 40) showing significant differences (p < 0.00001) between malaria-positive individuals (N = 33) and presumed asymptomatic negative individuals (N = 7) all collected in Gabon. This is the first successful application of the REEAD sensor system for the detection of malaria in saliva in a high-epidemic area and holds promise for the potential future use of REEAD for malaria diagnosis or surveillance based on non-invasive specimens in sub-Saharan Africa. Full article
(This article belongs to the Special Issue Recent Advances in Sensors for Chemical Detection Applications)
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<p>The REEAD sensor system. (<b>A</b>) The advantage of using pTOP1 as a biomarker for the detection of <span class="html-italic">Plasmodium</span> infections. Each parasite contains a high number of pTOP1 enzymes that each generate multiple DNA products without being consumed in the process. (<b>B</b>) The top panel shows the sequence and structure of the pTOP1-specific DNA substrate with the primer annealing site shown in blue. Cleavage–ligation by pTOP1 converts the substrate to a closed circle that is hybridized to a glass slide and amplified by RCA in the presence of (i) dNTPs with biotin-conjugated dCTPs for chemiluminescence readout (<b>left lower panel</b>) or (ii) without modified dNTPs followed by hybridization to fluorescently labeled probes for readout in a fluorescence microscope (<b>right lower panel</b>).</p>
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<p>Detection of pfTOP1 with chemiluminescence REEAD. (<b>A</b>,<b>B</b>) Bar charts showing the results of analyzing 1, 0.5, 0.25, 0.125, 0.0625, 0.03125 ng/µL pfTOP1 spiked in saliva by REEAD using the fluorescence readout detected using a microscope (<b>A</b>) or the chemiluminescence readout detected using a commercial CCD camera (<b>B</b>). As controls, samples without pfTOP1 (one with and one without saliva) were included. As a positive control, a sample with 1 ng/µL pfTOP1 without saliva was used. The identity of the samples is indicated below the bar charts. The experiments were performed in triplicates (indicated by each dot). To compensate for slide-to-slide variations, the signals obtained by either the microscope or chemiluminescence readout were normalized to the average of the samples with saliva and 0 ng/µL pfTOP1 and plotted as mean +/− standard deviation (SD). (<b>C</b>) Graphic depiction of the results obtained when testing the effect of removing unreacted DNA substrate before chemiluminescence readout. The identity of the samples is shown below the graph. Each experiment was repeated four to six times (indicated by dots). To compensate for slide-to-slide variations, the chemiluminescence REEAD signals were normalized to the average intensity of samples without pfTOP1 without exonuclease digestion (Exo) and plotted as mean +/− SD.</p>
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<p>Detection of <span class="html-italic">Plasmodium</span> in clinical saliva samples. (<b>A</b>) (<b>Left panel</b>) Graphical depiction of chemiluminescence REEAD results obtained when measuring extracts from two saliva samples from confirmed malaria positives and two saliva samples from presumed negative individuals prepared by 2–5 vortex repetitions with glass beads. The average of the results from two individual experiments is shown by horizontal lines. (<b>Right panel</b>) Raw data obtained with a CCD camera. (<b>B</b>) The results were obtained by analyzing 30 saliva samples from confirmed malaria-positive individuals and 31 saliva samples from presumed malaria negatives using chemiluminescence REEAD. The average of the results is shown by horizontal lines. Statistics are shown in <a href="#app1-sensors-24-07947" class="html-app">Supplementary Materials S1A</a>. To compensate for slide-to-slide variations, the chemiluminescence REEAD signals were normalized to the average of the signals obtained by analyzing negative samples vortexed 2 times (<b>A</b>) or to the average of the signals obtained by analyzing negative samples (<b>B</b>).</p>
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<p>Comparison of CCD camera and portable chemiluminescence reader (VPCIReader). (<b>A</b>) Left panel, schematic showing the construction of the chemiluminescence VPCIReader. Right panel, photo of the VPCIReader. (<b>B</b>,<b>C</b>) Bar charts showing the results of analyzing titrations of test DNA circles (diluted 0 to 32 times and indicated) by capturing the results of chemiluminescence REEAD by a commercially available CCD camera (<b>B</b>) or by the developed chemiluminescence VPCIReader. The readings of each of the three individual experiments are shown by dots. The sample marked “Neg” contains non-circularized DNA with a sequence matching the test DNA circles. To compensate for slide-to-slide variations, the chemiluminescence REEAD signals were normalized to the “Neg” sample and plotted as mean +/− SD. **** = <span class="html-italic">p</span> &lt; 0.0001, ordinary one-way ANOVA. “E” refers to an empty well only containing 5′-Amine REEAD primer.</p>
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<p>Detection of <span class="html-italic">Plasmodium</span> in clinical saliva samples by using VPCIReader. The results obtained by analyzing 33 saliva samples from confirmed malaria-positive individuals and 7 saliva samples from presumed malaria negatives using chemiluminescence REEAD. The average of the results is shown by horizontal lines. Statistics are shown in <a href="#app1-sensors-24-07947" class="html-app">Supplementary Materials S1B</a>. To compensate for slide-to-slide variations, the chemiluminescence REEAD signals were normalized to a well only containing 5′-Amine REEAD primer.</p>
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