Mass Spectrometry and Stable Isotopes in Nutritional and Pediatric Research
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About this ebook
A guide for scientists, pediatricians and students involved in metabolic studies in pediatric research
- Addresses the availability of modern analytical techniques and how to apply these techniques in metabolic studies
- Covers the whole range of available mass spectrometric techniques used for metabolic studies including Stable Isotope Methodology
- Presents the relevance of mass spectrometry and stable isotope methodology in pediatric research covering applications in Nutrition, Obesity, Metabolic Disorders, and Kidney Disorders
- Focuses on the interactions between nutrients and the endogenous metabolism within the body and how these factors affect the health of a growing infant
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Mass Spectrometry and Stable Isotopes in Nutritional and Pediatric Research - Henk Schierbeek
List of Contributors
Saskia J.H. Brinkmann
Department of Surgery
VU University Medical Center Amsterdam
Amsterdam
The Netherlands
Nikki Buijs
Department of Surgery
VU University Medical Center Amsterdam
Amsterdam
The Netherlands
Willemijn E. Corpeleijn
Department of Pediatrics
AMC
University of Amsterdam
Amsterdam
The Netherlands
Femke Maingay-de Groof
Department of Pediatrics
NoordWest Ziekenhuisgroep
Alkmaar
The Netherlands
Marita de Waard
Department of Pediatrics
VU University Medical Center
Amsterdam
The Netherlands
Hans Demmelmair
Division of Nutrition and Metabolic Diseases
Hauner Children's Hospital
Ludwig Maximilian University Munich
Munich
Germany
Margot Fijlstra
Department of Pediatrics
Beatrix Children's Hospital
Groningen University Institute for Drug Exploration (GUIDE)
University of Groningen
Groningen
The Netherlands
and
Department of Pediatrics
AMC
University of Amsterdam
Amsterdam
The Netherlands
Sander F. Garrelfs
Department of Pediatric Nephrology
Emma Children's Hospital/Academic Medical Center
University of Amsterdam
Amsterdam
The Netherlands
Jean-Philippe Godin
Analytical Sciences
Nestlé Research Center
Lausanne
Switzerland
Hidde H. Huidekoper
Department of Pediatrics
Center for Lysosomal and Metabolic Diseases
Erasmus Medical Center-University Hospital
Rotterdam
The Netherlands
and
Department of Pediatrics
Division of Metabolic Disorders
Academic Medical Center
University of Amsterdam
Amsterdam
The Netherlands
Berthold Koletzko
Division of Nutrition and Metabolic Diseases
Hauner Children's Hospital
Ludwig Maximilian University Munich
Munich
Germany
Stefanie M.P. Kouwenhoven
Department of Pediatrics
VU University Medical Center
Amsterdam
The Netherlands
Elvira Larqué
Department of Physiology
University of Murcia
Murcia
Spain
Gerdien C. Ligthart-Melis
Center for Translational Research in Aging and Longevity
Department of Health and Kinesiology
Texas A&M University
College Station
TX
USA
Gregorio P. Milani
Department of Pediatrics
Ca' Granda Ospedale Maggiore Policlinico
University of Milan
Milan
Italy
Michiel J.S. Oosterveld
Department of Pediatric Nephrology
Emma Children's Hospital/Academic Medical Center
University of Amsterdam
Amsterdam
The Netherlands
Dirk-Jan Reijngoud
Department of Pediatrics
Beatrix Children's Hospital
Groningen
The Netherlands
and
Center for Liver
Digestive and Metabolic Diseases
University of Groningen
University Medical Center Groningen
Groningen
The Netherlands
Denise Rook
Department of Pediatrics
Erasmus Medical Center
University Hospital Rotterdam
The Netherlands
Henk Schierbeek
Department of Pediatrics
AMC
University of Amsterdam
Amsterdam
The Netherlands
Chris H.P. van den Akker
Department of Pediatrics
AMC
University of Amsterdam
Amsterdam
The Netherlands
Johannes B. van Goudoever
Department of Pediatrics
AMC
University of Amsterdam
Amsterdam
The Netherlands
and
Department of Pediatrics
VU University Medical Center
Amsterdam
The Netherlands
Dewi van Harskamp
Department of Pediatrics
AMC
University of Amsterdam
Amsterdam
The Netherlands
Margriet Veldhorst
Department of Pediatrics
VU University Medical Center
Amsterdam
The Netherlands
Henkjan J. Verkade
Department of Pediatrics
Beatrix Children's Hospital
Groningen
The Netherlands
Ronald J.A. Wanders
Department of Pediatrics
Division of Metabolic Disorders
Academic Medical Center
University of Amsterdam
Amsterdam
The Netherlands
and
Department of Clinical Chemistry
Laboratory Genetic Metabolic Diseases
Academic Medical Center
University of Amsterdam
Amsterdam
The Netherlands
Frits A. Wijburg
Department of Pediatrics
Division of Metabolic Disorders
Academic Medical Center
University of Amsterdam
Amsterdam
The Netherlands
Introduction
High-precision mass spectrometric analyses are gaining popularity in many scientific disciplines, including metabolic kinetic studies in nutrition and pediatrics. Innovations in mass spectrometry and tracer administration techniques have made mass spectrometers the instruments of choice for the analysis of isotopic compounds. Techniques for measurements of deuterium and ¹⁸O, as well as for ¹³C isotopic analysis, have progressed. In particular, the coupling of liquid chromatography with isotope ratio mass spectrometry (LC-IRMS) has introduced new, highly sensitive analysis opportunities and opened new avenues for nutritional and pediatric research. An increasing number of researchers that use LC–IRMS in metabolic research have indicated the robustness of this technique; however, LC-IRMS is suitable for only ¹³C-isotopic measurements due to the lack of an existing LC interface for the introduction of other elements into the IRMS. A major challenge for the future, therefore, is the development of a technique that will enable the measurement of all common elements.
Although novel techniques have been developed and existing techniques have been improved, there are still new experimental disciplines left to uncover. The coupling of LC to IRMS was a major step toward further unraveling metabolic kinetics; this innovation was made feasible by the direct measurement of carbon isotopes in a wide range of low-molecular-weight compounds and macromolecules, ranging from naturally abundant to highly enriched samples. Strength of LC–IRMS lies in the straightforward analysis of underivatized components; its main drawbacks are the relatively low sensitivity (nanogram range) and its restriction to only ¹³C-isotopic samples. The low sensitivity can be a problem when measuring components in low concentrations, such as vitamins and hormones, or when samples are small, for example, in preterm infants or small rodents. Improvements in the sensitivity and robustness of LC–MS/MS systems have opened up new possibilities for studying macromolecules, such as peptides, hormones, vitamins, and small proteins, but a wide range of applications must still be developed in several disciplines using this technique.
Also, recently developed techniques, such as infrared spectroscopy for the measurement of isotopically labeled compounds, are gaining popularity in many biomedical applications. The most important advantages of these new techniques, relative to IRMS, are their low costs and simplicity. Novel developments for these instruments are based on the wavelength-scanned cavity ring down spectroscopy (WS-CRDS analyzer). These instruments are as precise as IRMS but use less sample (e.g., when measuring ¹³C values in CO2). This technique requires little or no sample preparation, the analysis time is short (a few minutes), and minimal skill is needed to operate the machines; however, these instruments still need to be thoroughly tested in biomedical research applications.
Even with the advances made thus far, there are still many topics in metabolic kinetic studies that have yet to be elucidated. However, the growing availability and decreasing costs of stable isotopes will make it increasingly possible to broadly explore human metabolic kinetics worldwide.
The aim of this book is to present the relevance of mass spectrometry and stable isotope methodology in nutritional and pediatric research. Applications for the use of stable isotopes with mass spectrometry cover carbohydrate, fat, protein, and specific amino acid metabolism, energy expenditure, and the synthesis of specific peptides and proteins.
The main focus of these studies is on the interactions between nutrients, endogenous metabolism within the body, and how these factors affect the health of a growing infant. Considering that the early imprinting of metabolic processes has huge effects on metabolism (and thus functional outcome) later in life, research in this area is important and is advancing rapidly.
The book should be a guideline for scientists, analytical chemists, biochemists, clinical chemists, and pediatricians, as well as for medical graduate students and lecturers involved in metabolic studies in life sciences.
This book shows the availability of modern analytical techniques and how to apply these techniques in practice and covers the entire range of available mass spectrometric techniques used for metabolic studies.
The chapters show applications of study models as well as provide detailed information about tracer administration, sampling, the selected analytical techniques, and calculations.
List of Abbreviations
Chapter 1
Mass Spectrometry Techniques for In Vivo Stable Isotope Approaches
Jean-Philippe Godin¹ and Henk Schierbeek²
¹Analytical Sciences, Nestlé Research Center, Lausanne, Switzerland
²Department of Pediatrics, University of Amsterdam, Amsterdam, The Netherlands
1.1 Introduction
Interest in the use of light-stable isotopes (i.e., Carbon-13 or ¹³C; Nitrogen-15 or ¹⁵N; deuterium or ²H; Oxygen-18 or ¹⁸O) has become widespread over the past 20 years in archeology [1], climatology [2], biochemistry [3], geochemistry [4], forensics [5, 6], and food adulteration [7, 8]. These various scientific domains share a striking commonality, which is the use of similar analytical approaches to look at the level of light-stable isotopes in various chemical components and matrices, either at natural abundance or after tracer incorporation. Among these domains, particularly in nutritional and pediatric studies, the combination of modern mass spectrometry (MS) and light-stable isotopes has been very effective for studying the effect of diet and disease on protein, carbohydrate, lipid, and energy metabolism. In vivo assessment of specific pathways using stable isotopes is unique and offers powerful insights about metabolic pathways and changes in metabolic fluxes in clinical studies.
In practice, once the nutritional hypothesis is defined, the clinical investigator needs to find an adequate model that can compensate for the metabolic complexity of the in vivo processes. It becomes obvious that the isotopic data generated has to be combined with physiological inputs, which results in information that characterizes metabolic changes and individual needs (i.e., from pregnancy [9] to elderly women [10]). As with studies in adults or in pregnant women, in pediatric studies, light-stable isotopes are used to study various metabolisms (i.e., carbohydrate, protein, lipids, and energy) [11, 12]. However, pediatric studies are limited by several parameters, such as (1) ethical and technical constraints around collecting biological fluids (i.e., breath, plasma, saliva, urine, and feces) especially in neonates and infants; (2) the low amount of biomaterial collected; (3) the invasiveness of the methods (the study protocol must be non- or semi-invasive, limiting kinetic studies and accessibility to tissues); and (4) difficulty recruiting and convincing parents to enroll their infants, limiting the number of subjects per study and increasing the pressure on the analytical precision of the method used. Consequently, the biological samples are precious and the choice of analytical technique/method is crucial. Both must be integrated in the clinical workflow from the beginning, to design fit-for-purpose analytical stable isotope approaches to deliver the clinical outcome with the expected precision to detect an effect.
The information obtained with stable isotopes in metabolic studies provides meaningful insights compared to a simple concentration measurement in blood. Briefly, stable isotope tracers allow the calculation of metabolic fluxes between organs and give a dynamic view on metabolism rather than a static one as measured by analyte concentrations [13]. For example, these tracers enable quantification of the sum of a dynamic process of several physiologic mechanisms such as carbohydrate absorption and digestion, hepatic glucose production by the liver, peripheral tissue uptake (i.e., muscle, gut, and brain), and other biochemical pathways such as the glycolysis/oxidation. To glean deeper scientific insights and decipher small effects of nutrients or to characterize phenotypes (i.e., lean, obese with or without type 2 diabetes [14]), stable isotopes offer a unique tool for better understanding glucose homeostasis compared to glycemic response [15, 16].
MS is the most versatile and comprehensive analytical technique that can be used to tackle multiple scientific questions in several fields, including physics, pharmaceutical sciences, medicine, environmental sciences, and nutrition (to mention a few). Modern MS is a common tool that is used in many laboratories, but the number of teams able to examine the incorporation and dilution of light-stable isotopes for pediatric and nutritional studies is limited. With the increased recognition of the unique metabolic information gathered from the use of light-stable isotope tracer methods in metabolic studies, MS instruments have become the field's workhorse. In parallel to MS, other techniques, such as nuclear magnetic resonance (NMR) [17], magnetic resonance spectroscopy [18], Fourier transform infrared red spectroscopy [19], or cavity ring-down spectroscopy [20] are also used to measure light-stable isotopes in various in vivo applications, but these techniques are less common. Typically, these instruments do not achieve the sensitivity and precision that can be obtained with MS instruments.
We focus here on modern MS approaches that enable us to examine light-stable isotope levels in organic molecules, in particular isotope ratio mass spectrometry (IRMS) and modern (organic) MS. The diversity of peripherals such as gas chromatography (GC), liquid chromatography (LC), or elemental analyzer (EA) hyphenated to MS instruments illustrates the variety of molecules to analyze. The molecules of interest in nutritional and pediatric studies are mostly amino acids, simple carbohydrates, lipids (such as cholesterol, fatty acids, and triglycerides), urea, ammonia, water, organic acids, glycerol, breath CO2, and macromolecules such as proteins and DNA.
The goal of this chapter is to provide a general overview and summary of the capabilities of various MS techniques in combination with light-stable isotopes for in vivo assessment of metabolic fluxes. It is neither a historical overview nor is it a detailed instrumental and methodological summary of all the isotopic techniques used for nutritional and pediatric studies.
1.2 Nomenclature for Light-Stable Isotope Changes
1.2.1 Natural Abundance
Many chemical elements have more than one isotope. Molecules and ions with different isotopes of the same chemical element possess slightly different physical and chemical properties. Light-stable isotopes occur naturally at abundances of approximately 1.11% for ¹³C, 0.37% for ¹⁵N, 0.20% for ¹⁸O, and 0.015% for ²H. However, isotope ratios are not constant on earth and can vary depending on the location on earth. There are some exchanges between the ocean, biosphere, and lithosphere due to kinetic and equilibrium isotope effects, leading to subtle but significant variations in nature [2]. Isotopic fractionation between light and heavy isotopes occurs when chemical reactions are not completed or when multiple products are formed, and those isotopes are unevenly distributed among the reactants and products. Isotopic fractionations can be quantitatively predicted only when the mass balances, kinetics, and equilibrium isotope effects associated with all the relevant reactions are well described [21]. For isotopic analysis, isotopic fractionation is a critical parameter to look at during chemical reactions. Rieley discussed this effect and showed that mass balance equations can be used to obtain the true isotopic abundance [22].
In plants, during photosynthesis, metabolized products become relatively depleted in ¹³C compared to environmental CO2. A variation of the ¹³C/¹²C ratio in different plant species is observed. On the one hand, there are plants (i.e., cereal grains, rice, sugar beets, and beans) that only use the three-carbon pathway (C3-plants) for carbon fixation, and they have a ¹³C/¹²C ratio (expressed as δ¹³C) of about −28‰ VPDB (Vienna Pee Dee Belemnite). On the other hand, C4-plants (i.e., corn, millet, sugar cane, and many grasses) also use C4 carbon fixation and are more enriched in ¹³C. Their ¹³C/¹²C ratio (δ¹³C) is about −13‰ VPDB [23].
In clinical studies, the variation of natural isotopic abundances due to diet can lead to subtle variations that may increase the variability of the study results. It is therefore recommended that during a clinical study with stable isotopes, subjects should follow clear instructions about diet and lifestyle [24].
1.2.2 Tracer
In the last few decades, the use of light-stable isotopes was preferred to radioisotopes for biomedical and metabolic studies, as they lacked radiation emission and are safer to handle. This is particularly relevant for the pediatric population, where the use of radioisotopes is extremely limited for safety reasons. Several different stable isotope tracers can be safely administered to children. For example, [¹⁵N]-glycine and [1-¹³C]-leucine were simultaneously administered in preterm infants for measuring whole-body protein turnover [25, 26]. Cogo et al. infused [¹³C]-palmitic acid and [²H3]-leucine for 3 h and [²H5]-glycerol for 5 h to measure protein turnover and lipolysis in critically ill children who were 10 years old [27]. This concept of multiple tracer administration is only achievable if the samples are analyzed with MS or NMR instruments.
As defined by Wolfe and Chinkes, a tracer is a compound that is chemically and functionally identical to the naturally occurring compound of interest (tracee) but is distinct in some way that enables detection
[28]. ¹³C and ¹⁵N tracers are commonly employed to trace amino acids, whereas, by design, lipids and small carbohydrates can be artificially enriched with ¹³C, deuterium, or both. Therefore, many components labeled with light-stable isotopes (i.e., tracers) have been produced and are now commercially available. Deuterium-labeled tracers are generally the cheapest of the light-stable isotope tracers. The major drawback, however, is that deuterium atoms are labile (i.e., exchangeable with unlabeled and surrounding hydrogen atoms). Deuterium-labeled water (heavy water) is an excellent tracer for measuring total body water (and body composition) and, when associated with 18-Oxygen (²H2¹⁸O), allows for the assessment of total energy expenditure (TEE) [29–31], among other applications. Although there is a widespread use of the double-labeled water method, the availability of water enriched with ¹⁸O at 10 at% or 98 at% (as isotopic purity) is low due to its limited worldwide production, making it very expensive (about 10 times higher than deuterium-enriched water). Furthermore, the reactivity of oxygen with many other components makes it very challenging to manufacture ¹⁸O tracers.
1.2.3 Isotopic Ratio and Isotopic Enrichment Measurements
Of note, there is no single expression of isotopic enrichment in metabolic studies, as reported by Wolfe and Chinkes [28]. Expressions will vary with the mass spectrometers used (IRMS instruments vs organic mass spectrometers), the level of variation in the isotopes, and the metabolic models used to assess the final clinical outcomes.
1.2.3.1 Delta Notation Measured by Isotope Ratio Mass Spectrometry
The abundances of isotopic ratios, such as ¹³C/¹²C, ¹⁸O/¹⁶O, ²H/¹H, and ¹⁵N/¹⁴N, are always measured relative to the isotope ratio of a specifically selected reference material. The reference standard materials are VPDB for carbon [32], Vienna Standard Mean Ocean Water (VSMOW or VSMOW2) for oxygen and hydrogen, and laboratory air for nitrogen [33]. Since these primary reference materials are quite limited or do not exist anymore, other easily accessible international stable isotope reference materials are also commercially available from the International Atomic Energy Agency (IAEA, Vienna, Austria) in different isotopic values.
δ values are unitless numbers such as the isotope ratios itself, but due to the small differences measured, δ values are usually expressed in parts per thousand, per mil, or ‰ (equation 1.1).
1.1
equationwhere R is the ratio between the minor (heavier) isotope of the element to the major (lighter) isotope (i.e., ¹³C/¹²C).
Of note, most organic components at natural abundance are depleted in the heavy isotope form relative to the reference standard, leading to negative δ values.
In some metabolic applications that use labeled water (i.e., ²H2O) to measure body composition or use double-labeled water (i.e., ²H2¹⁸O) to assess total energy expenditure, the parts per million (ppm) unit is also reported. In this case, the transformation is as follows (equation 1.2):
1.2
equationwhere δ²H is the per mil ²H with respect to the international reference VSMOW or VSWOW2. The factor 0.00015576 is the ²H/¹H ratio of VSMOW [34].
1.2.3.2 Expressions of Isotopic Enrichment
In metabolic studies, once the tracer has been administered, the tracer-to-tracee ratio (TTR) is commonly used to report the isotopic enrichment. Alternative units reported in peer-reviewed papers are atom percent excess (APE, %) or molar percent excess (MPE, %). These units represent the amount of tracer as a ratio of the sum of tracer and tracee. As described by Wolfe and Chinkes [28], the tracer and tracee are indistinguishable from a metabolic point of view but distinguishable by using MS, measuring different isotopologues (i.e., components differing only in their isotopic composition such as [1-¹³C]-leucine vs [1-¹²C]-leucine). TTR is calculated based on mass spectrometer data using the following formula (equation 1.3):
1.3 equation
where rsa is the ratio of tracer/tracee in the sample (after administration of the tracer), rbk is the ratio of tracer/tracee in a background sample (before administration of the tracer), "A is a skew correction factor that varies with the isotope, and
n" is the number of labeled atoms. For the ¹³C tracer, A is 0.0111, whereas for the ¹⁵N tracer, A is 0.0037, as A is equal to the natural abundance of the element.
Finally, TTR can also be transformed into MPE or into APE using equations (1.4) and (1.5):
1.4 equation
1.5
equationwhere Ctotal is the total number of carbons in the molecule of interest and Clabeled is the number of carbons labeled in the molecules.
The APE and MPE expressions are similar when no extra carbons are added to the compound of interest as in liquid chromatography–isotope ratio mass spectrometry (LC–IRMS). However, in gas chromatography–combustion isotope ratio mass spectrometry (GC–C-IRMS), the compounds are mostly derivatized, implying that the additional carbon needs to be taken into account to obtain the enrichment of the intact molecule [35].
Of course, there are other possible transformations of isotopic enrichments that can be used in specific metabolic models. As example, for measuring the fractional synthesis rate (FSR) or the absolute fractional synthesis (ASR) in muscle after infusion of a stable isotope tracer (i.e., with ¹³C6-phenylalanine), the isotopic ratio of phenylalanine extracted from muscle biopsy, as measured by IRMS (i.e., δ¹³C, ‰), can be transformed into a TTR value using equation (1.6):
1.6
equationwhere δsa is the isotopic abundance (IRMS data) of the sample [36].
To calculate the isotopic enrichment using gas chromatography–mass spectrometry (GC–MS) or liquid chromatography–mass spectrometry (LC–MS), the baseline unlabeled sample and labeled samples (after administration of the tracer) are subtracted (as described by Wolfe) or can be assessed using a mathematical matrix of mass isotopomer distribution, as reported by Fernandez et al. [37], to determine the true isotopomer distribution.
1.2.3.3 Normalization of Isotopic Ratio Expressed with δ Unit
In order to calibrate raw δ values to international references so that interlaboratory comparisons can be carried out, it is crucial to transform raw δ values (data from the IRMS instrument) into normalized δ values for accurate and comparable isotopic determination. In this context, a specific protocol (known as isotopic normalization) needs to be put in place during isotopic analysis. The requirements for isotope normalization have increased dramatically not only with the commercialization of new technology to compare technique performance but also due to the broad types of applications and the increasing number of laboratories that can carry out isotopic analysis. Paul et al. described different approaches to normalize isotopic ratios [38]; normalization with two or more certified standards produces less errors than normalization carried out with only one. In most metabolic tracer studies, isotopic normalization is not mandatory (but advised), since an excess of isotopic enrichment (see Section 1.2.3.2) is the appropriate way to express results.
1.3 Mass Spectrometry Techniques
The basic principle of MS is to produce ions from organic molecules, to separate these ions by their mass-to-charge ratio (m/z), and to detect them qualitatively and quantitatively by their respective m/z and abundance. As schematically represented in Figure 1.1, different options exist to measure light-stable isotopes with MS.
Illustration depicting Typical elements (i.e., separation mode, ion source, analyzer, and detector) used to measure light-stable isotopes in metabolic studies.Figure 1.1 Typical elements (i.e., separation mode, ion source, analyzer, and detector) used to measure light-stable isotopes in metabolic studies.
1.3.1 Isotope Ratio Mass Spectrometry
The measurement of natural isotopic abundances and tiny variations of isotopic enrichments in organic molecules requires a very specific technique known as IRMS. The isotope ratio mass spectrometer, initially developed by Nier, is based on a multicollector magnetic sector mass spectrometer [39]. The theory and practice of IRMS are reviewed in detail elsewhere [40, 41] and will not be reviewed here. Briefly, the isotope ratio mass spectrometer is made of several modules, such as a tight-electron impact ion source, a magnetic sector, and several Faraday cups to simultaneously monitor several ions. To determine small differences in isotopic ratios, parameters such as sensitivity, signal stability, and counting statistics are key parameters that enable high-precision measurements [42]. The IRMS device, or the so-called gas-IRMS,
is designed to measure the isotope ratio of light-stable isotopes, such as ¹³C, ¹⁵N, ¹⁸O, ³⁴S, and ²H, of organic molecules that were previously transformed into gases, such as CO2, N2, CO, SO2, and H2. Continuous-flow-IRMS is the most common approach (as opposed to the dual isotope system with off-line conversion of organic molecules), due to the ease of sample transformation. Several interfaces are used to produce these gases. High-precision isotopic analysis of solid and liquid bulk samples is achieved using an EA or thermal conversion-elemental analyzer (TC/EA) coupled to an IRMS device for measurement of the ¹³C, ¹⁵N, ²H, and ¹⁸O isotopes, whereas GC and LC conjugated to an IRMS device allow for measurement of the isotopic ratio of specific compound(s) after chromatographic separation.
1.3.1.1 Bulk Stable Isotope Analysis
Bulk analysis of ¹⁵N, first demonstrated by Preston and Owens in 1983, is based on bulk isotopic analysis [43]. Its principle is straightforward since the bulk sample (i.e., powder or liquid) is weighed in a tin capsule that is introduced into a heated combustion interface through an autosampler (i.e., a carousel). Within the heated furnace, the organic bulk material is transformed into gases (i.e., CO2 and N2). These gases are carried out in a flow of helium gas stream and introduced into a heated reduction furnace where nitrous oxides are converted into N2 (Figure 1.2). Then, any excess O2 and water are removed before introducing the helium stream into the IRMS ion source. By design, the EA-IRMS measures ¹³C and ¹⁵N isotopic abundances. The isotopic precision of EA-IRMS, expressed as standard deviations (SD) of δ, is lower than 0.3‰ for ¹³C and ¹⁵N isotopes for sample amounts greater than 50 nmol of an element, or an amount of nitrogen (as urinary urea and ammonia after adequate processing) from 30 to 150 µg.
Schematic of an elemental analyzer for EA-IRMS coupling.Figure 1.2 Schematic representation of an elemental analyzer for EA-IRMS coupling. Source: Muccio and Jackson [44]. Reproduced with permission of Royal Society of Chemistry.
To examine the ²H and ¹⁸O isotopic ratios of bulk samples, the oldest approach was based on the cryodistillation of biological samples to produce H2 gas, followed by a reduction with catalyzers (i.e., zinc and platinum), whereas for ¹⁸O isotope determination, the produced CO gas was equilibrated overnight with unlabeled CO2 present in the water solution. These processes were time consuming and required large volumes of sample. However, in the 2000s, a new commercial system became available to both measure isotopes with smaller amounts of material and utilize an automated system. In this case, the organic material was not combusted but quantitatively pyrolyzed (at 1420 °C in a glassy carbon reactor within a TC/EA) to produce H2 and CO gases that were introduced into the ion source of the IRMS device through a helium stream as the carrier gas. Technically, the ability to measure the ²H/¹H ratio in a helium (He; m/z 4) stream was challenging, due to the large He peak in the ion source. There is a little overlap of this high abundant peak onto the m/z 3 Faraday cup collector. Because of the high intensity of the helium peak in comparison to the intensity of the ²H/¹H peak, this contributed significantly. The solution was to add a retardation lens into the m/z 3 Faraday cup collector. Moreover, H3+ is formed in the ion source, caused by the reaction H2+ + H2 → H3+ + H•. This also contributes to the ²H/¹H peak but can be accounted for by the so-called H3+ factor. Practically speaking, the H3+ factor needs to be assessed daily to obtain precise and accurate isotopic ratios [40]. In this case, the IRMS device is equipped with such specific collectors and is able to accurately measure both ²H and ¹⁸O isotopes (Figure 1.3). Interestingly, the system allows for the simultaneous detection of both isotopes in the same run, limiting the final volume drawn from the patient and increasing the analytical throughput (typical run time is lower than 6 min per sample). The isotopic precision of the TC/EA-IRMS is about 2.0‰ for δ²H and 0.3‰ for δ¹⁸O. This system is particularly relevant in pediatric studies, where only a small volume of biological fluid (i.e., urine, blood, or saliva) is available.
Illustration depicting TC-EA/IRMS chromatogram with H2 Q1 and C18O peaks after injection of water sample.Figure 1.3 Typical TC-EA/IRMS chromatogram with H2 and CO peaks after injection of water sample.
Finally, a third bulk stable isotope analysis (BSIA) approach was developed for breath ¹³CO2 isotopic enrichment. Analytically, this is accomplished by a combination of headspace sampling and loop injection onto a GC column capable of resolving different gases, such as CO2 and N2, connected to an IRMS device (GC-IRMS). In these conditions, the combustion furnace is off. The analytical measurement per se is very straightforward and the isotopic precision is lower than 0.3‰ for δ¹³C. This method, known as the ¹³C-breath test [45], allows for the determination of specific clinical outcomes, such as the presence of Helicobacter pylori after ingestion of labeled urea or measurements of fat digestion and gastric emptying [46–48].
1.3.1.2 Compound-Specific Isotopic Analysis
One common feature of BSIA and compound-specific isotopic analysis (CSIA) is the use of helium as a carrier gas to transport the targeted gases (i.e., CO2, N2, H2, and CO). However, with CSIA, a chromatographic separation of the targeted compound is carried out prior to the transformation of the organic molecules into gases. The separation can be performed either by GC or LC.
1.3.1.2.1 Compound-Specific Isotopic Analysis with Gas Chromatography–Isotope Ratio Mass Spectrometry
This approach was first coined isotope ratio monitoring-GCMS
by Matthews and Hayes [49], but today is named continuous-flow-isotope ratio mass spectrometry (CF-IRMS). One of the first technical considerations of CSIA by GC is to reliably convert online organic molecules into gases while maintaining the chromatographic separation and resolution achieved on the GC column. Combustion interfaces (for ¹³C and ¹⁵N applications) used after GC separation were developed in the early 1980s, whereas pyrolysis furnace applications (for ²H and ¹⁸O) were built in the 1990s (Table 1.1). In contrast to IRMS, which is a highly specialized mass spectrometer, the GC system used for GC-IRMS coupling is a standard commercial and generic instrument. Most GC methods are applicable to isotopic measurements in terms of analytical conditions, with helium (He) as the carrier gas.
Table 1.1 Typical light-stable isotopes used in metabolic studies and characteristics of IRMS instruments hyphenated to gas chromatography for measuring light-stable isotopes
Source: Sessions [40]. Reproduced with permission of John Wiley and sons.
a Sensitivity expressed in nanomole of the analyzed element injected to get a precision close to the value listed in this table.
Principle of Gas Chromatography Combustion Isotope Ratio Mass Spectrometry
For measuring either the ¹³C or ¹⁵N isotopic ratios of selected components, GC–C-IRMS fits the purpose. Briefly, after adequate derivatization of polar compounds, the derivatized components are injected into a capillary gas chromatographic column with an autosampler. Individual compounds are carried by a helium stream and separated chromatographically according to their volatility and their interaction with the stationary phase. Then, the helium carrier introduces the compounds into a combustion furnace. This consists of a ceramic tube, typically with an inner diameter of 0.5 mm, with metal wires (CuO/NiO/Pt), which is heated to 940 °C, where each compound is converted into CO2, water, and nitrogen oxide (NOx) gases. In order to get rid of these NOx gases, a reduction furnace (heated to 650 °C and containing Cu and Pt wires) is installed in series, where nitrogen oxide gases are transformed into N2O and NO2. Water is removed by a Nafion® water trap, and finally a small fraction of the gases (in the helium stream) is introduced into the IRMS ion source (Figure 1.4). The remainder of the gas stream is diverted to the atmosphere via a split. By design, the IRMS can only accept a maximum of 0.4 mL/min of helium carrier gas [41].
Schematic of GC interface for GC-C-IRMS coupling.Figure 1.4 Schematic representation of GC interface for GC–C-IRMS coupling. Source: Muccio and Jackson [44]. Reproduced with permission of Royal Society of Chemistry.
Principle of Gas Chromatography Pyrolysis Isotope Ratio Mass Spectrometry
For measuring deuterium and Oxygen-18 in compounds after a chromatographic separation, a pyrolysis furnace is used instead of a combustion furnace. The pyrolysis furnace is heated to 1400 °C [50]. At this temperature, organic components are transformed into H2 and CO gases when oxygen is present. The high temperature for pyrolysis requires a high-purity Al2O3 (alumina) reactor tube. At such a temperature, alumina tubes are sensitive and leaks may develop over time. Within gas chromatography–pyrolysis isotope ratio mass spectrometry (GC–P-IRMS), alumina tubes have to be replaced more often than reactors used in combustion systems (for ¹³C or ¹⁵N). In addition, many users and suppliers recommend conditioning the pyrolysis reactor from time to time with injections of organic solvent or via backflushing with CH4/He gas. This likely prevents deposits of carbon inside the alumina tubes, which decreases its efficiency [51]. One additional difference with the combustion interface is the absence of Nafion membranes, since pyrolysis of organic compounds does not produce water. Of note, halogen atoms induce contaminants for the pyrolysis process and memory effects generated may impact the accuracy and precision of deuterium isotopic measurements produced by GC–P-IRMS [52].
Sample Preparation and Gas Chromatography Separation
The fundamental aspect of GC is to separate the different components and to provide baseline chromatographic resolution between peaks [53]. This is of paramount importance for precisely and accurately measuring isotope ratios, such as in doping applications [54]. High-resolution chromatographic columns may separate molecules with different isotope contents, meaning that a component with one deuterium or one Carbon-13 atom will be chromatographically separated from its nonlabeled counterpart. This factor can be a real chromatographic issue when complex matrices are analyzed; therefore, adequate peak integration and background signals are important parameters to consider [55]. In such circumstances, fast GC–C-IRMS [56] and two-dimensional GC-IRMS [57, 58] may not only improve chromatography separation but also may have other challenges for isotopic abundance determination. There are only a few papers reporting such approaches, and none were metabolic or pediatric studies.
For high-precision isotopic analysis in biological fluids (i.e., plasma, urine, and saliva) or tissue (i.e., muscle), regardless of the targeted isotopes, several critical steps need to be considered. The most important ones are sample preparation and gas chromatographic separation, although isotopic standardization and processing are also critical [59]. Sample preparation may sound trivial for many analytical chemists using classical mass spectrometers, but for high-precision isotopic analysis, due to the low specificity of IRMS and the possible isotopic fractionation that may occur, these steps require careful attention. Meier-Augenstein [53] provided an extensive review of the conditions for ¹³C and ¹⁵N isotopic analysis by GC with detailed protocols. Briefly, a typical GC sample preparation protocol implies (1) an isolation step(s) of the targeted component from complex matrices (i.e., plasma, muscle), (2) the choice of an internal or external standard with a known isotopic ratio, and (3) a derivatization step to ensure that the targeted molecule is volatile. During these steps, it is important to pay close attention to isotopic mass discrimination so as to avoid isotopic fractionation of the target component [22]; if not, this may affect the accuracy and precision of the approach and increase the error in the final results. Although isotopic fractionation is not a major issue for an in vivo tracer approach (as opposed to areas such as forensic sciences), careful attention to the choice of the reagents of derivatization is recommended in order to obtain reliable, robust chromatographic conditions and to limit the isotopic dilution from large and unnecessary atoms from the derivative itself. For example, silylation reagents are very popular in GC–MS for derivatization of polar groups, such as hydroxyl, amino, and thiol groups. However, for GC–C-IRMS, silylation reagents that add trimethylsilyl (TMS) or tert-butyl-dimethylsilyl (tBDMS) groups bring a large number of additional carbons into the targeted component, affecting the final isotopic ratio of the targeted derivatized component. It may also produce siliceous deposits on the oxidation catalyst, reducing its surface area and gas flow through the combustion reactor [60]. For amino acid isotopic analysis, optimization of the derivatization conditions is important, as described by Corr et al. [61], and various derivatives have been used. Of note, most of them were applied for ¹³C and ¹⁵N isotopes, a few for deuterium, and none for ¹⁸O. The absolute isotopic abundance of free plasma ¹⁵N amino acids varies, either due to the sample preparation method (via ion-exchange chromatography) and/or due to amino acid metabolism, which affects the ¹⁵N/¹⁴N isotopic ratios (i.e., deamination and transamination, as discussed by Metges and Petzke [62]). When deuterated labels are used, loss of one or more isotopic labels due to hydrolysation techniques is very common. One of the most popular derivatizing techniques for amino acids is using an alkyl chloroformate reagent. Several papers have described these techniques [63–68]. For small carbohydrates, different analytical strategies were developed to optimize the sample preparations. For example, Jackson et al. proposed a common derivative suitable for both GC–MS and GC–C-IRMS, allowing for precise measurements of deuterium and ¹³C atoms with a glucose alkylboronate derivative [69]. Other analytical strategies, such as online solid-phase microextraction with GC–C-IRMS, were also optimized to reduce isotopic fractionation and applied in a clinical study that looked at acetate and butyrate isotopic enrichments in plasma samples [70].
1.3.1.2.2 Compound-Specific Isotopic Analysis with Liquid Chromatography–Isotope Ratio Mass Spectrometry
For decades, several developments have attempted to couple LC and IRMS (LC–IRMS) [71]; however, the technical challenge was to quantitatively transform, without any isotopic discrimination, organic molecules solubilized in a buffer (mostly organic with reverse-phase chromatography) into CO2 gas and to then extract the gas from a liquid into a helium stream that was introduced into the ion source of the IRMS. Progress was only achieved in 2004 when a commercial interface was developed that facilitated LC coupling with IRMS (LC–IRMS) [72].
The interface to couple the LC and IRMS, proposed by Thermo Finnigan (named LC Isolink®) or Elementar (named LiquiFace®), is based on the chemical wet oxidation of organic molecules to produce CO2 gas in aqueous solution within a heated reactor (at 99 °C), where phosphoric acid, sodium peroxodisulfate, and the LC eluent are mixed together. Then, the inorganic eluent containing CO2 is carried through a separation unit where CO2 gas is extracted and selectively transferred into a helium stream. Water is removed from the gas bypassing through two Nafion membranes, and finally the He stream enriched with CO2 gas is introduced into the IRMS ion source (Figure 1.5). By design, some level of oxygen is also introduced into the ion source, leading to some detrimental effects on the lifetime of the filament and on isotopic accuracy and precision [73, 74]. A new interface has been described recently, which has the ability to measure both ¹³C and ¹⁵N isotopes. It is based on a modified high-temperature combustion total organic carbon analyzer and the proof of concept was reported by measuring caffeine samples [75].
Schematic of LC interface for LC-IRMS coupling.Figure 1.5 Schematic representation of LC interface for LC–IRMS coupling. Source: Muccio and Jackson [44.]. Reproduced with permission of Royal Society of Chemistry.
Compared to GC–C-IRMS, the LC–IRMS interface is less versatile in terms of potentially analyzable isotopes, since only the ¹³C/¹²C isotopic ratio can be routinely determined. On the other hand, LC–IRMS is unique in that it has two modes of isotopic analysis: one via an LC column to separate components to obtain their individual ¹³C/¹²C ratios and a second mode of isotopic analysis named flow-injection analysis (FIA) [76]. This mode of isotopic analysis is for both low-molecular-weight and high-molecular-weight components (i.e., albumin, insulin) [77], and is straightforward and similar to EA-IRMS for hydrosoluble molecules (solubilized in aqueous nonorganic buffer). The main advantage of FIA-IRMS, compared to EA-IRMS, is its sensitivity. The amount of material required for analysis is a 100 times less.
By design, LC–IRMS is only suitable for high-precision isotopic analysis of hydrosoluble molecules, such as amino acids, volatile fatty acids, alcohols, some phenolic acids, some simple carbohydrates, nucleotides, peptides, and proteins [78]. To analyze these components, the key features of the analytical methods must be compliant with the following analytical constraints: (1) only inorganic buffers can be used; (2) only water-soluble components can be analyzed; (3) the total flow rate (i.e., LC column plus the flow rate of acid and oxidant) must be lower than 700 µL/min; and (4) the acidic mobile phase achieves more efficient CO2 extraction from the liquid.
One theoretical advantage of LC versus GC is the sample preparation method, which is simpler in LC. With