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HPF
Catalog No.
C3384
HPF is a stable fluorescent reagent for detecting high levels of reactive oxygen species (ROS).
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HPF(Hydroxyphenyl fluorescein) is a cell-permeable aromatic amino-fluorescein derivative that has little intrinsic fluorescence.HPF reacts with intracellular peroxynitrite through hydroxyl radicals, generating intense green fluorescence. It offers high specificity and stability: oxidation occurs only in the presence of highly reactive oxygen species (hROS) such as hydroxyl radicals, peroxynitrite, and hROS generated by peroxidase/H₂O₂ systems. HPF does not react with hypochlorite ions, nitric oxide, hydrogen peroxide, superoxide, or other oxidants. Upon oxidation, HPF converts to the highly fluorescent molecule fluorescein, with excitation/emission peaks at 490/515 nm, making the direct detection of highly reactive biological radicals simple and intuitive. HPF can be used in fluorescence microscopy, high-throughput imaging systems, fluorescence plate readers, or flow cytometry.
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- 2. CHEN Chao, WANG Yi, et al. "Bifunctional nanozyme activities of layered double hydroxide derived Co-Al-Ce mixed metal oxides for antibacterial application." Journal of Oceanology and Limnology. Mar. 21, 2020.
- 3. Wang J, Wang Y, et al. "Exploring the bactericidal performance and application of novel mimic enzyme Co4S3." J Colloid Interface Sci. 2020;561:327–337. PMID: 31771873
- 4. YiWangacd, ChaoChenabcd, et al. "Bifunctionalized novel Co-V MMO nanowires: Intrinsic oxidase and peroxidase like catalytic activities for antibacterial application." Applied Catalysis B: Environmental Available online 5 October 2019, 118256. PMID: 31666990
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 424.4 |
| Cas No. | 359010-69-8 |
| Formula | C26H16O6 |
| Synonyms | Hydroxyphenyl fluorescein; 3'-p-(Hydroxyphenyl) fluorescein |
| Solubility | ≤20mg/ml in ethanol; 20mg/ml in DMSO; 20mg/ml in dimethyl formamide |
| Chemical Name | 3'-hydroxy-6'-(4-hydroxyphenoxy)-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one |
| SDF | Download SDF |
| Canonical SMILES | OC(C=C1)=CC=C1OC2=CC3=C(C=C2)C4(C(C=CC=C5)=C5C(O4)=O)C6=CC=C(O)C=C6O3 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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