An Ethnomedicinal Survey of Medicinal Plants used by Folk Medicinal Practitioners of different villages in Tangail district, Bangladesh to treat Jaundice and Allergy

International Conference On Updates on Natural Products in Medicine and Healthcare Systems 06 July, 2013 At CSS Ava Center, 82 Rupsha Strand Road, Khulna-9100, Bangladesh Organized by: Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh Funded by: INSPIRE, British Council In Association with University of Wolverhamton, England, UK Proceedings of International Conference on Updates on Natural Products in Medicine and Healthcare Systems ISBN: 978-984-33-7625-1 (web version) ISBN: 978-984-33-7626-8 (Print version) Formatted and Compiled by: Abdullah Mahmud-Al-Rafat & Apurba Majumder Cover and Graphic Design Ahmad Ullah Copyright @ Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh International Advisory Committee  Prof Satyajit D Sarker, University of Wolverhampton, Wolverhampton, UK (Chair)  Dr Lutfun Nahar, De Montfort University, Leicester, UK (Co-chair)  Prof Pulok Mukherjee, Jadavpur University, Kolkata, India  Prof Runner Majinda, University of Botswana, Gaborone, Botswana  Prof Abdu Razag Auzi, Tripoli University, Libya  Prof Robert Nash, Phytoquest Ltd, Aberystwyth, UK  Prof Alexander I. Gray, University of Strathclyde, Glasgow, UK  Prof Marcel Jaspars, University of Aberdeen, Aberdeen, UK  Prof Muraleedharan G. Nair, Michigan University, USA  Dr Randolph Arroo, De Montfort University, Leicester, UK  Prof Emidio Da Cunha, Departamento de Farmácia e Biologia, Universidade Estadual da Paraíba, Brazil  Dr Khozirah Saari, University Putra Malaysia (UPM), Selangor, Malaysia  Dr. Rehana Afrin, Tokyo Institute of Technology, Tokyo, Japan  Prof Abbas Delazar, Tabriz University of Medical Sciences, Tabris, Iran  Dr Hossein Nazemiyeh, Tabriz University of Medical Sciences, Tabris, Iran  Dr Omar Janneh, Coventry University, Coventry, UK  Prof Virginia Lanzotti, Università degli Studi di Napoli Federico II , Naples, Italy  Prof Wieslaw Oleszek, Institute of Soil Science and Plant Cultivation Pulawy, Poland. 1 International Conference Updates on Natural Products in Medicine and Healthcare Systems International Conference Organizing Committee       Prof Dr. Mohammad Fayek Uzzaman, Hon‟ble Vice-Chancellor, Khulna University, KhulnaChief Patron Prof Dr. Sheikh Md. Enayetul Babar, Head, Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-Patron Prof. Dr. Khondoker Moazzem Hossain, Biotechnology Discipline, Khulna University, Khulna -Member and Genetic Engineering Prof. Dr. S M Mahbubur Rahman, Biotechnology and Genetic Engineering Discipline,Khulna University, Khulna -Member Dr. Md. Morsaline Billah, Associate Professor, Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh -Convener. 2 International Conference Updates on Natural Products in Medicine and Healthcare Systems Message Convener International Advisory Committee It is a great pleasure and honour to welcome you all to the international conference, „Updates on Natural Products in Medicine and Health Care Systems„, sponsored by the INSPIRE programme from the British Council, to be held in Khulna, Bangladesh. This conference builds on the successes of national seminars and workshops on mangrove plants from the Sundarbans, held in Khulna University, during the past couple of years. The main objective of this day-long international conference is to evaluate and update the knowledge on the contributions of natural products, plants and plant-products from the Sundarbans for example, to medicine and healthcare. With participation of renowned as well young natural products scientists from India, UK, and all parts of Bangladesh, I am sure that this conference will provide a unique platform to exchange views on advances in research in natural products especially in relation to promoting plant-based medicines and primary healthcare. The conference will also highlight the current demands and market situation of plant-based medicinal products in Bangladesh, and offer some pointers as to how to improve the quality of such products. It is expected that several new approaches, e.g., nano-science, chemometrics, principal component analysis, modern extraction technologies, and bioassay developments, will be at the heart of several oral and poster presentations. I wish all participants a stimulating, thought-provoking and an enjoyable conference. Prof Satyajit D Sarker, B. Pharm (Hons) M. Pharm Ph.D FHEA Professor of Pharmacy Editor-in-Chief, Phytochemical Analysis & Department of Pharmacy University of Wolverhampton, United Kingdom. 3 International Conference Updates on Natural Products in Medicine and Healthcare Systems Message Convener International Conference Organizing Committee In recent years, the medical value and industrial stimulus have provided by the availability of a number of plant-based drugs, isolated from plant materials of oriental folk-medicine. It is likely that there remains the possibility of discovering novel compounds with significant and valuable pharmacological properties to be isolated from plant materials, and that, incidentally, clues to some of them may still be found in the folk-medicine of indigenous people. In particular, the rich and comparatively uninvestigated abundant flora of South Asia especially Bangladesh require careful and systematic investigation, and it is gratifying to see that collaborative efforts are underway among the institutions of South-South and North-South cooperation. This international conference is an outome of research partnership between Khulna University, Bangladesh and University of Wolverhampton, United Kingdom. We hope that everyone will find everyone's contribution not only in the respective session of their preferences but also the other sessions would fulfill everyone's expectation. However, with respect to the initially foreseen sessions, we have been obliged to introduce several changes, that reflect the number of contributions submitted in the various subfields of natural product chemistry, and thus your present scientific interests. We hope the conference will contribute to maintain active scientific discussions through exchange of ideas, theories, methodologies, expertises and the recommendations during the sessions and aside. The conference could not be organized without the financial support of International Strategic Partnerships in Research and Education (INSPIRE) project, British Council. We would like to thank British Council for the contribution towards success of the conference. We are also indebted to the International Conference Organizing Committee, student volunteers, other committees and the chairs of the sessions as well as to members of CSS Ava Center, Khulna especially Md. Mottasim Billah, for their respective and really appreciated contributions. We wish all the participants an excellent conference and a pleasant stay in Khulna. Dr. Md. Morsaline Billah Biotechnology and Genetic Engineering Discipline Khulna University, Khulna-9208, Bangladesh. 4 International Conference Updates on Natural Products in Medicine and Healthcare Systems Message Head Biotechnology and Genetic Engineering Discipline Khulna University, Khulna-9208, Bangladesh It is my privilege and honour to invite all of you to the upcoming International Conference on Updates on Natural Products in Medicine and Healthcare Systems which will be held from 06 July in Khulna, Bangladesh. The conference is first of its kind in Biotechnology and Genetic Engineering Discipline, probably in Khulna University as well. Therefore, we have the pleasure to arrange something meaningful and effective towards the rational use of plants with medicinal values, scientific exploitation and exploration towards plant-based medicinal systems and knowledge driven drug discovery using modern techniques. I hope the participants will not only foster excellent discussions and exchanges on the technical challenges of medicinal systems based on natural products, but also address the issues related to integration of traditional medicine into modern medicine. The conference is expected to provide a number of high quality scientific contributions in the form of plenary, oral and poster presentations. I am sure that we will have an excellent conference which will provide sound information on natural products, technological progress in the field of plant-based drug development, reliability and challenges of traditional medicine. Moreover, it is your chance to establish networks! Be our guest in Khulna, a beautiful city in the south west of Bangladesh, close to the Sundarbans (the largest mangrove forest and home of Royal Bengal Tigers) and the Bay of Bengal. I send you a warm welcome and I am looking forward to seeing you all in the conference. Sheikh. Md. Enayetul Babar, PhD Professor and Head, Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, Bangladesh. 5 International Conference Updates on Natural Products in Medicine and Healthcare Systems Program Schedule 8.30-9.30 Inaugural Session 9.30-10.00 Tea Break 10.00-10.40 Thematic Lecture on Recent Contributions of Natural Products in Medicine and Healthcare Systems by Prof A B M Faroque, Department of Pharmaceutical Technology, University of Dhaka, Bangladesh. Technical Session I Parallel Session 1 Conference Room1 Chair: Prof Satyajit D Sarker, Deputy Head, Department of Pharmacy, School of Applied Sciences, University of Wolverhampton, Wolverhampton, UK. 10.45-11.15 Plenary Lecture1: Professor Arup Mukherjee, Ph.D., Head, Division of Pharmaceutical & Fine Chemicals Technology, Department of Chemical Technology, University of Calcutta, India “ Plant Drug Nanomedicines for Pharmacokinetic Development and Commercialization”. 11.15-11.45 Plenary Lecture2: Prof Sitesh C Bachar, Department of Pharmaceutical Technology, University of Dhaka, Dhaka, Bangladesh “Importance and Impacts of Medicinal Plant Cultivation in Bangladesh”. 6 International Conference Updates on Natural Products in Medicine and Healthcare Systems 11.45-12.15 Plenary Lecture3: Dr Mukhlesur Rahman, Programme leader in Pharmaceutical Science, School of Health, Sports and Bioscience University of East London Stratford Campus, London, UK "Antibacterial lead compounds from medicinal plants" . 12.15-12.40 Oral Presentation1: Dr. Mohammad Salim Hossain, Chairman, Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bnagladesh “Prostaglandin J 2; Natural Bioactive Lipid Modules Adipocyte Inflanmmation”. 12.40-13.00 Oral Presentation2: Dr. Md. Moklesur Rahman Sarker, Chairman, Department of Pharmacy, Faculty of Science, “Immunostimulating Primeasia University, activity of Ayurvedic Dhaka, preparations Bangladesh Saribadi, Anantamool Salsa, Mrittunjoy and Saribadyarista in vitro”. Parallel Session 2 Conference Room 2 Chair: Prof M. S. K. Choudhuri, Department of Pharmacy, Jahangirnagar University, Dhaka, Bangladesh. 10.45-11.15 Plenary Lecture1: Prof Pulok Mukherjee, Director, School of Natural Product Studies , Department of Pharmaceutical Technology, Jadavpur University,Kolkata, India “Globalization of traditional medicine (TM) – Safety, efficacy, therapeutic and clinical evidences.” 7 International Conference Updates on Natural Products in Medicine and Healthcare Systems 11.15-11.45 Plenary Lecture2: Dr Subhash C Mondal, Pharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University, India “Scope of drug discovery and the role of Stress in the Formation of Lead Molecules in Medicinal Plants”. 11.45-12.15 Plenary Lecture3: Dr Emdad Hossain, Professor, Pharmacy College, Azamgarh, Uttar Pradesh, India “Anti-anxiety activity of methanol extract of Dregea volubilis leaves”. 12.15-12.40 Oral Presentation1: Md. Harun Al Rashid, Pharmacognosy and Phytotherapy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, India “BERBERINE LOADED NANOPARTICLES: AN IMPLICATION FOR ANTIMICROBIAL THERAPY ‟‟. 12.40-13.00 Oral Presentation2: Dr. M Mizanur Rahman, Department of Biotechnology and Genetic Engineering, Islamic University, Bangladesh “A Comprehensive Ethnopharmacological and Phytochemical Update Review on Terminalia arjuna”. 13.00-14.00 8 Prayer Break, Lunch and Poster Session International Conference Updates on Natural Products in Medicine and Healthcare Systems Technical Session II Parallel Session 1 Conference Room1 Chair: Prof Bidyut K Datta, Department of Pharmaceutical Technology, University of Dhaka, Dhaka, Bangladesh. 14.00-14.30 Plenary Lecture 4: Dr. Begum Rokeya, Professor & Chief Research Officer, Department of Pharmacology, BIRDEM, Dhaka, Bangladesh “Medicinal plants in combating the emerging threats of Type 2 Diabetes”. 14.30-15.00 Plenary Lecture 5: Dr. Achintya Saha, Department of Chemical Technology, University of Calcutta, Kolkata, India “Contraceptive property assessments of standardized formulations of root extract and isolated molecule of Piper betle”. 15.00-15.20 Oral Presentation 1: Mehdi Bin Samad, Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh “Investigation on antidiabetic action of aqueous extract of Aloe vera on Normal and Streptozotocin Induced Type 2 Diabetic Rats”. Technical Session III Chair: Prof Dr. J M A Hannan, Head, Department of Pharmacy, North South University, Dhaka, Bangladesh. 15.20-15.50 Invited Lecture 1: Dr. Asish Kumar Das, Associate Professor, Pharmacy Discipline, Khulna University, Khulna, Bangladesh “Macrophage-derived protein kinase C-δ is a key molecule for pancreatic β-cell destruction in streptozotocininduced diabetes”. 9 International Conference Updates on Natural Products in Medicine and Healthcare Systems 15.50-16.10 Oral Presentation 1: Fahad Hussain, Department of Pharmacy, Noakhali Science and Technology University (NSTU), Sonapur, Noakhali, Bangladesh “In vitro Thrombolytic Effects of Roots of Four Medicinal Plants Available in Bangladesh”. Parallel Session 2 Conference Room2 Chair: Prof Abu Shara Shamsur Rouf, Chairman, Department of Pharmaceutical Technology, University of Dhaka, Dhaka, Bangladesh. 14.00-14.30 Plenary Lecture 4: Prof Dr. M Aftab Uddin, Chairman, Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh "Systemic evolution of traditional medicine".. 14.30-15.00 Plenary Lecture 5: Prof M. S. K. Choudhuri, Department of Pharmacy, Jahangirnagar University, Dhaka, Bangladesh. 15.00-15.20 Oral Presentation 1: Hasan Imam, Lecturer, Department of Pharmacy, School of Science, Primeasia University, Dhaka, Bangladesh “Alpha Amylase Enzyme Inhibitory and Anti-inflammatory Effect of Lawsonia inermis”. 15.20-15.40 Oral Presentation 2: Md. Mahmodul Islam, Department of Pharmacy, Noakhali Science and Technology University, Sonapur 3814, Noakhali, Bangladesh “JATROPHA GOSSYPIIFOLIA: A POTENTIAL CARDIO PROTECTIVE PLANT”. 10 International Conference Updates on Natural Products in Medicine and Healthcare Systems 15.40-16.00 Oral Presentation 3: Zahid Hossain, Managing Director, ZAP Natural Product Ltd. Bangladesh “An unexplored Treasure of Spirulina and Medicinal Herbs”. 16.00-16.30 Prayer Break, Poster Session and Tea Break Technical Session IV Chair: Prof Dr. K M Hossain, Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, Bangladesh. 16.30-17.00 Plenary Lecture 1: Prof A B M Faroque, Department of Pharmaceutical Technology, University of Dhaka, Bangladesh “Traditional Medicine for an Affordable and Positive Global Health”. 17.00-17.30 Plenary Lecture 2: Prof Satyajit D Sarker, Deputy Head, Department of Pharmacy, School of Applied Sciences, University of Wolverhampton, Wolverhampton, UK “Our interaction with „Sweet Sultan‟ and others to date”. 17.30-18.00 11 Concluding Session and Discussion International Conference Updates on Natural Products in Medicine and Healthcare Systems Plenary Presentation Traditional medicine for an affordable and positive global health. Professor A B M Faroque* Former Dean, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh & President, Bangladesh Pharmaceutical Society Abstract There is a tremendous development in last 100 years in the health care sector. Many new drugs have been discovered and some of them have brought almost a revolution in treating human ailments. Thus sufferings of the ailing people have lessened, longevity has increased and overall health indices have improved. But accessibility and affordability of the poor to medicine is still a major issue to be solved globally. There is no single solution or magic bullet towards these questions because these issues are not only complicated, but also complex. Against this backdrop place and utility of Traditional Medicine (TM) has emerged as a complementary vehicle towards affordable healthcare to many un-served or underserved poor population across the world, especially in the developing and least developed countries. According to WHO (2004), the average per capita spending on pharmaceuticals in the developed countries is nearly $400, but that in developing countries is only about $4. Even when someone gets infected with HIV/AIS, a patient in the developed country spends an average of $300 per annum, while a similar patient in the developing country can spend only $5. These examples indicate that it is now high time to integrate TM to modern medicine which will deliver a holistic synergy leading to an affordable, available, effective and safe healthcare for all citizens of the world. But TM has deficiencies too, majors of which are (i) lack of validation, (ii) lack of standardization, (iii) lack of integration into the health care infrastructures, (iv) lack of appropriate policies, (v) lack of regulatory control over the misuse and abuse etc. Earlier it was widely believed that TMs are very economic to use and that is the main reason behind TM‟s popularity amongst the poor. But current trends that have been set by some of the traditional medical men and TM manufacturers, reflects that in many a cases TMs are (i) much costly than the modern medicine counterparts, (ii) much misused and abused due to illegitimate practices by both the prescribers and the manufacturers, and (iii) even in many cases modern active pharmaceutical ingredients are being added to „popular‟ TMs without any declaration in the label. These bottlenecks must be removed must be removed for the sake of integration of TMs into more meaningful and positively responsible horizons of national and global health in future days. Keywords: Traditional medicine; Global health; Developing countries; Pharmaceuticals. 12 International Conference Updates on Natural Products in Medicine and Healthcare Systems Plenary Presentation Contraceptive property assessments of standardized formulations of root extract and isolated molecule of Piper betle Plaban Bhattacharya, Arup Mukherjee, Achintya Saha*1 1 Department of Chemical Technology, University of Calcutta, 92, A.P.C. Road, Kolkata-700009, India Abstract Rapidly expanding population and practice of unsupervised prolonged steroidal contraceptive usage are the leading cause of a number of life threatening disease conditions. The present study has been carried out to evaluate the ethno-contraceptive use of roots of Piper betle (PBR), followed by toxicity studies. The hydro-ethanolic extract of PBR loaded in 2-hydroxypropylbeta-cyclodextrin (BCD) was administered in female rats from day 1 to 7 of pregnancy. PBRBCD (1:1, w/w) formulation exhibited optimum contraceptive potency at the dose level of 250 mg/kg p.o. It showed impeded estrogenic and progestational activities and prolonged the length of estrus cycle, altered release of gonadal hormones and gonadotropins. Detailed pharmacological analyses followed by chronic toxicity studies were performed to predict the reversible nature of the developed phytopharmaceutical. Pharmacometabonomics analyses by 1H NMR revealed the favorable serum lipid profiling of the developed formulation over the ethnomedicinal preparation. The histological and biochemical estimations detected the reversible contraceptive potential. Bioactivity guided fractionation was carried out for identification of active constituents present in PBR. Piperolactam A (PL) is isolated from the ethyl acetate fraction of PBR. PL-BCD complex showed 100% anti-implantation activity at 5 mg/kg/day for first three days of pregnancy (n=10). PL might be useful as a promising lead compound of next generation female contraceptive. Keywords: Contraceptive activity, Piper betle, pharmacometabonomics, piperolactam A, toxicity studies. 13 International Conference Updates on Natural Products in Medicine and Healthcare Systems Plenary Presentation Systemic evolution of traditional medicine M. Aftab Uddin, Ph.D Professor and Chairman, Department of Genetic Engineering and Biotechnology, University of Dhaka Abstract Natural products are organic compounds that are formed by living systems, which have some biological effect within the cell or organism that is responsible for their production or on other organisms. In the history of medication, it is the natural products that were the only healing substances for health and healthcare, in nutrition, and prevention of diseases. The first use of medicinal plants for healthcare or to cure illness or diseases is not exactly known to anybody. But it is sure that the search of some remedy for some discomfort is a patrimony and observation of self-medicative behavior in animals, where sick animal utilize certain plants that they usually ignore, over past decades that attributed to whole human race about medicinal plants and which we shall know and preserve. In reality, animal behaviorists, ecologists, pharmacologists, anthropologists, geochemists, and parasitologists have all contributed to this truly multi-faceted discipline. From different literature it is quite clear that the discovery of medicinal plants were mainly based on serendipity. Most times the discoveries were simply results of the search of new types of food to maintain good state of health by trial and error. In searching food Man found the poisons, the allergens, and other produced somewhat different effects such as they increase the sweat, they defecate with greater extent, eliminate joint pain, and other discomfort effect, cure for fevers and headaches etc., etc. The first written text on the use of medicinal plants is about 4000 years old Sumenian clay tablet that records remedies of many illnesses. Herbalism is thought to have started some 60,000 years back. By 400 years B.C., Hippocrates, the father of medicine, who tackled medicinal plants in a more scientific manner than ever before? The long and the oldest history of Indian medicinal system are well established with written records going back around 3000 years, known as Ayurveda, has left written reference from 800 A.C., where they describe 800 species. Currently more than 6,000 plant species are known and used in Indian Systems of Medicine (Ayurveda, Siddah, Unani and Homeopathy in addition to modern Medicine). Natural products of plants, terrestrial and marine animals, and microorganism derived preparations were being used by these systems. It is the purpose of this paper to provide the most recent information an updates on natural products in medicine and healthcare system. Keywords: Natural products; Ayurveda; Unani; Homeopathy. 14 International Conference Updates on Natural Products in Medicine and Healthcare Systems Plenary Presentation Plant Drug Nanomedicines for Pharmacokinetic Development and Commercialization Professor Arup Mukherjee*, Ph.D Head, Division of Pharmaceutical & Fine Chemicals Technology, Department of Chemical Technology, University of Calcutta, 92 A.P.C.Road, Kolkata- 700009, India. Abstract The progress in human history is intricately linked to the scientific utilization of natural resources. Human health is one area in which, substantive knowledge in plant drugs remained enormously rewarding through the ages. Modern molecular interpretation for plant medicines however could only be established in 1806, when, Friedrich Sertürner a pharmacist, isolated the bioactive principle morphine, from Papaver somniferum. A long list of purified plant drug molecules was since developed. Some of the more recent examples in that line include, Taxol, against cancer from Taxus brevifolia and Artemisinin against multi-drug resistant falciparum malaria from Artemisia annua. In most cases though, the bioactivities for known chemical entities of plant origin are well established but, their therapeutic applications are marred due to chemical incompatibilities and pharmacokinetic limitations. Taxol for example is water insoluble and need a solubilizer cremophor EL, which in itself causes severe adverse reactions. Artemisinin, in addition has a very low biological half life. Nanomedicine is a converging discipline with unbound potentials in the health care sector. Robust scientific studies have already established that particles in nanosize can modulate biological distribution in vivo or be targeted based on surface chemistry. We have used a variety of techniques to develop different plant bioactive nanomedicines. Andrographolide for example was nanoparticulated in cationic modified Poly (d,l-lactic-co-glycolic acid), PLGA for applications in cancer chemotherapy. Quercetin conjugated in gold nanoparticle was devised as a chemotherapeutic in drug resistant visceral leishmaniasis. Silymarin flavonolignans drug delivery devices were developed to mitigate irreversible paracetamol hepatotoxicity. Mysticism around nomedicines is now only historic and several pharmaceuticals including those from plant bioactives are in market place. „Abraxane‟ a cremophore free albumin based taxol nanoparticles is one successful anticancer agent in market. Polyethylene glycol conjugated artemisinin nanosystems were also developed for enhanced solubility and sustained release. It is likely that a slow down in drug discovery that is seen in recent past can only be rejuvenated from the low cost nanomedicine developments from different natural known chemical entities. Keywords: Taxol; Artemisin; Nanomedicine; Bioactive compound. 15 International Conference Updates on Natural Products in Medicine and Healthcare Systems Plenary Presentation Medicinal plants in combating the emerging threats of Type 2 Diabetes Begum Rokeya*, Ph.D Professor & Chief Research Officer, Department of Pharmacology, BIRDEM, 122 Kazi Nazrul Islam Avenue, Dhaka-1000, Bangladesh. Abstract Diabetes mellitus is now a major public health problem in the developed as well as developing countries. The International Diabetes Federation predicts that the number of people living with diabetes will rise from 366 million in 2011 to 552 million by 2030. The burden of Type 2 diabetes is enormous due to increasing prevalence, chronic course, disabling complications and high treatment cost. Diabetes management studies show that conventional antidiabetic agents like sulfonylureas are the least durable agents followed by metformin and thiazolidinediones. Therefore, search for improved antidiabetic drug has been continued. Over the last several years the incretin-based therapies have got significant importance although they are very much expensive. Therefore, search for improved drug(s) against diabetes has remained a major goal among the biomedical researchers. Since approach of the scientists for synthesis of these drugs has not yet brought expected results, many of them in recent years, are focusing their attention on natural compounds to find, at least a lead, for antidiabetic agents. It is well known that Bangladesh and neighboring countries have a rich tradition in indigenous herbal medicine. A number of medicinal plants and their formulations are being used for the treatment of diabetes in the traditional system as well as in ethnomedicinal practices which needs to be scientifically evaluated. Following a standardized approach collaborative studies between BIRDEM and Chemistry Department of Dhaka University are being conducted on plant materials for more than two decades. So far 84 plants from Bangladesh and neighboring countries have been screened for antidiabetic properties. Screening results showed that majority of these plants have got significant blood glucose lowering effect, although they differed in the nature of the animal models and prandial states. Some of the active extracts were tested for long-term glucose and lipid lowering effects. A number of sub-fractions and compounds isolated from the active extracts were tested on pancreatic islets and beta cells for insulin releasing effects. Inhibition of carbohydrate absorption through gastrointestinal tract in rats by inhibiting disaccharidase activities, effects on GI motility and inhibition of platelet aggregation indicated the presence of various principle(s) in hypoglycemic plant extracts. Based on the obtained results controlled clinical trials have been performed with a couple of plants. .Keywords: Medicinal plant; Type 2 Diabetes; Ehtnomedicine; Antidiabetic agent. 16 International Conference Updates on Natural Products in Medicine and Healthcare Systems Plenary Presentation Anti-anxiety activity of methanol extract of Dregea volubilis leaves Emdad Hossain*1, Dilip Tiwari1, Rishikant Tripathi1, Subhash C. Mandal2, Jayanta Kumar Gupta2 1 Pharmacy College, Azamgarh, Uttar Pradesh 276128, India. 2 Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India. Abstract Advancement in modern science and technology has enhanced the quality of human life. At the same time, very fast life pattern with stressful condition all through the day has created a lot enormous stress in modern life, has resulted in the incidence of variety of psychiatric disorders like anxiety. These are recurrently accompanied by somatic symptoms such as tachycardia, dizziness, palpitations, sweating, nausea, irritability, insomnia, and impaired concentration. Severe side effects and addiction liabilities associated with long term administration of widely prescribed synthetic drugs have aroused the attention of researchers towards natural resources. Dregea volubilis (Family: Asclepiadaceae) is extensively used for insomnia as traditional medicine in different parts of India. Anti-anxiety activity study was done with methanol extract of Dregea volubilis leaves (MEDV) using light-dark model and elevated plus maze model as experimental protocol. Four groups of Wistar rat containing six animals in each group were taken for the study in each model. MEDV at two dose levels (200 and 300 mg/kg body weight) as test drug and diazepam at dose 4 mg/kg body weight as standard drug were administered orally. Vehicle was given to the control group. MEDV in both doses significantly enhanced the time spent in light chamber in a dose dependent fashion (p<0.001) in light-dark model as well as it enhanced the number of entries and time spent in the open arms significantly (p<0.001) in elevated plus maze model which were comparable with the standard. It can be concluded that the leaves extract possess significant anti-anxiety activity and the study will help for utilization of MEDV in different herbal preparation. Keywords: Herbal medicine; anti-anxiety; Dregea volubilis. 17 International Conference Updates on Natural Products in Medicine and Healthcare Systems Plenary Presentation Antibacterial lead compounds from medicinal plants Md Mukhlesur Rahman* School of Health, Sports and Bioscience, University of East London, Stratford Campus, Water Lane, London E 15 4LZ, United Kingdom. e-mail: m.rahman@uel.ac.uk, Tel: +44-2072234299 Abstract In the era of antibiotic resistance, natural products notably plants, microbes and marine organisms continue to be an important source of lead compounds for drug discovery. Indigenous medicinal plants from different countries are under-exploited for antibacterial drug discovery whilst more than 80% of the population in Asia and Africa depend on medicinal plants for the primary health care. The overall aim of the plenary lecture is to provide a brief update of my own research in natural products chemistry. The methods used in the bioassay directed isolation and identification of lead compounds involved extraction, initial fractionation and further purification followed by identification. Each of the plants was Soxhlet extracted sequentially with hexane, chloroform and methanol. Based on activity, the extracts were further fractionated by VLC followed by purification by a range of chromatographic techniques including gel filtration, column chromatography, preparative TLC and HPLC. The compounds were identified by spectroscopic techniques including MS and NMR (both 1D and 2D). The antimicrobial activity was determined by microdilution titre assay. Extensive phytochemical investigation on the above plants led to the isolation and identification of a wide range of compounds- terpenes, steroids, lignan, simple phenolics, flavonoids and isoflavonoids including some new compounds. Among the compounds, a new isoflavone, 4',5,7-trihydroxy-8-(1,1-dimethylprop-2-enyl)-isoflavone showed highest activity against Staphylococcus aureus. Its MIC was found to be 1.57 g/ml (0.005 mol). In conclusion, the isolation of potent antimicrobial agents has reasonably justified the medicinal uses and inspires scientists for further research. Keywords: Antibiotic investigation. 18 resistance; Lead compound; Medicinal plant; Phytochemical International Conference Updates on Natural Products in Medicine and Healthcare Systems Plenary Presentation Globalization of traditional medicine (TM) – Safety, efficacy, therapeutic and clinical evidences Pulok K Mukherjee, M. Pharm, PhD, FRSC Director, School of Natural Product Studies, Dept. of Pharmaceutical Technology, Jadavpur University, Kolkata-700032, India. Abstract Globalization of traditional medicine (TM) is necessary for health care with assessment of its safety, efficacy, therapeutic and clinical evidences. New technology and science has developed many techniques and systems to raise the traditional medicine compounds for global existence. Establishment of global and/or regional regulatory harmonization is necessary for its development and promotion through scientific validation. Traditional medicines are mostly indigenous to a country or region. It has developed through daily experiences and mutual relationships between people and nature. For thousands of years they have cured diseases and prevented illness, thereby maintaining health. The huge secondary metabolite resources of medicinal plants- their phyto-constituents are widely respected for their unique chemical and biological features, and are gaining global acceptance because they offer natural ways to treat diseases and promote healthcare. The global health survey in 1970, boost up the use of ancient systems of medicine for better healthcare. Scientists around the world are searching for medicinal plants as alternative medicine and their potential in health care. The development of natural products requires the confluence of modern techniques and integrated approaches related to their research in various fields of science through International coordination and cooperation. When adopted outside of its traditional culture, traditional medicine is often called complementary and alternative medicine. It is a fact that inappropriate use of traditional medicines or practices can have negative or dangerous effects and emphasis is being given on its scientific validity to ascertain the efficacy and safety of the practices and medicinal plants used in this system. To combat with the growing market demand, there is urgency to expeditiously utilize and scientifically validate more medicinally useful plants globally – which needs globalizing local knowledge and localizing global technologies in this area through international collaboration and co-operation. Keywords: Traditional medicine; Healthcare system; Therapeutics; Natural products. 19 International Conference Updates on Natural Products in Medicine and Healthcare Systems Plenary Presentation Our interaction with „Sweet Sultan‟ and others to date Satyajit D. Sarker1* and Lutfun Nahar2 1 Department of Pharmacy, University of Wolverhampton, MA Building, Wulfruna Street, Wolverhampton WV1 1LY, West Midlands, United Kingdom. 2 Faculty of Science, Liverpool John Moores University, James Pearsons Building, Byrom Street, Liverpool L3 3AF, United Kingdom. Abstract „Sweet Sultan‟ (Centaurea moschata L.) belongs to the genus Centaurea (family: Asteraceae alt. Compositae), which comprises ca. 600 species of herbaceous thistles and thistle-like flowering plants. Most of the Centaurea species are native to the Old World, but also cultivated elsewhere. Centaurea moschata and many other species of this genus are wellknown for their medicinal properties, and folklore uses for the treatment of several ailments including cancer, diabetes, diarrhoea, hypertension, malaria, rheumatism and tumour. A variety of secondary metabolites, belonging to the classes of alkaloids, flavonoids, lignans, sesquiterpenes and simple phenolics, with potential anticancer activities, particularly against colon cancer cell-lines, have been reported from different Centaurea species. The presentation will present an overview of our work on C. moschata and related species of this genus to date, with particular emphasis on compounds with potential anti-colon cancer activity. Keywords: Centaurea; Centaurea moschata; Asteraceae; CaCo-2; colorectal cancer. ___________________ *Address from 1 October 2013: School of Pharmacy and Biomolecular Sciences, Faculty of Pharmacy, Liverpool John Moores University, James Pearsons Building, Byrom Street, Liverpool L3 3AF, United Kingdom 20 International Conference Updates on Natural Products in Medicine and Healthcare Systems Plenary Presentation Importance and Impacts of Medicinal Plant Cultivation in Bangladesh. Sitesh C Bachar* *Professor, Department of Pharmaceutical Technology, University of Dhaka, Dhaka -1000, Bangladesh. Abstract Bangladesh has a long tradition of indigenous herbal medicinal systems based on the rich local plant diversity which is considered as a very important component of the primary health care system. And more than 75% of its population is getting their primary health care through Ayurved, Unani, Homeopath, Herbal and other traditional medicine systems. A good number of such traditional manufacturers are using about 550 medicinal plants from various sources. And only 10 percent of these are available in Bangladesh and rest 90 percent is being imported from broad. A medicinal plants cultivation in the rural area of northern part of Bangladesh at Monga affected areas especially in the small strips of land in the homestead or village road side of marginal or low income people which made them economically self sufficiency creating impacts in environment and increasing social values and integrity. Keywords: Herbal medicine; Medicinal plants; Ayurved; Unani; Homeopath. 21 International Conference Updates on Natural Products in Medicine and Healthcare Systems Plenary Presentation Scope of drug discovery and the role of Stress in the Formation of Lead Molecules in Medicinal Plants Subhash C Mandal* *Pharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700032, India. Abstract Natural products have been long regarded as mainstay of drug discovery programmes. India from its rich valuable source of traditional system “Ayurveda” offers an array of drug discovery leads to be useful for the treatment of several ailments. Diabetes has spread its toxic tentacles all through and every sections of the society. Recent failures in the discovery process of new chemical entities have made natural product researchers to look into history of herbal drug use for search of leads. Reverse pharmacology approach forms the major weapon of search of anti-diabetic leads. Long documented/undocumented use of different unexplored medicinal plants by the ethnic groups forms the first step in the search of anti-diabetic lead. Expectation of low toxicity for humans from plants used traditionally can be easily accepted as these plants have been thoroughly tested owing to their use since ages. With the increasing hectic lifestyle which has made the pathogenesis of diabetes so complex. Modern medicine attempts to use a single compound to hit single target of a particular pathway for combating the related disease which may not prove to be a long-lasting management strategy. On the other hand, traditional medicines exert synergistic effects due to its galaxy of phytoconstituents hitting multiple targets and thus acting through multi-mechanistic pathway. Henceforth, drug discovery spectrum from traditional medicine has now two basic objectives a) Discovery of new chemical entities from plants mentioned in literature of traditional systems of medicine and b) Development of standardized polyherbal formulations based on traditional medicines formulae. However, to gain global acceptance and better therapeutic management for such metabolic disorders it is essential that such drug discovery approaches be integrated with modern biology to evolve a holistic system biology approach for the management of diabetes. Through this integration, drug discovery from Ayurveda can make a strong impact on the western world. Traditional medicine has a long history of serving mankind all over the world. Phytochemicals obtained from traditional medicinal plants are presenting an exhilarating panorama for the expansion of modern therapies to combat against knotty diverse ailments. Amongst most precious medicines from phytosources are analgesic agents like morphine and codeine from Papaver sominiferum, antimalarial agents like quinine and antiarrythmic agent like quinidine from the bark of Cinchona species, antitumor agents namely vincristine, vinblastine from Catharanthus roseus, taxol from taxus species, hepatoprotective agents mainly silymarin from Silybum marianum and antidiabetic agent like gymnemic acid from Gymnema sylvestre still playing important role in orthodox medicine. As the interest in medicinal plants mainly resides on its secondary metabolites as health aid the research on secondary metabolite biogenesis, isolation, preclinical and clinical studies has been accelerated in recent years. The local environment sets the agenda of plant growth and yields in a given habitat and regulates the performance of individuals. Production of active ingredients in medicinal plants also gets influenced by the environment factors. Samples from various agroclimatic conditions collected at different stages of plant growth and covering the various plant parts needs to be investigated for obtaining the best out of a medicinal plants. Keywords: Lead Molecules; Drug discovery; Ayurveda; Anti-diabetic. 22 International Conference Updates on Natural Products in Medicine and Healthcare Systems Plenary Presentation Traditional Medicine in Newer Drug Delivery Systems Sukalyan Kumar Kundu1,2 1 2 Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, Bangladesh. Research Fellow, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India. Abstract The most primitive ground of treatment is governed by the use and/or practice of traditional medicine, which is actually of different preparations or formulations from the plant, animal and/or mineral origin available through our surroundings. Even in the present world the use of phytomedicines is quite popular and thereby widespread in the most of the population around the world. This is an integral part of the healthcare system in the developing and underdeveloped countries of the world. Interestingly, many developed countries are also inclining to the use of different traditional medicines alongside with their most popular synthetic/allopathic treatment regime. Though traditional medicines ensures patient‟s compliance due to their different attributes like natural origin, less toxicity, more availability, ease of preparation, no regulatory barriers, etc., they often cause harm to health for their improper and unregulated use. In many occasions we find the aqueous extract of different plants and/or plant parts like roots, leaves, barks, etc., are more beneficial for the different ailment purposes. For the application of traditional medicine we mostly prefer either oral or topical route. The polar constituents extracted from the natural origin are often larger in molecular size and thus show retarded transport into the targeted organ due to lipid-bilayer conformation of biological membrane. On the other hand, the non polar constituents of the plant origin show decreased solubility in the aqueous body fluid and thereby also cause limited bioavailability on oral ingestion resulting in poorer efficacy. For these reasons, sometimes we use larger doses of plant extracts or actives for availing the desired effect, which in turn cause toxic and/or lethal effects in some cases. Hence, it is very much necessary to develop some newer drug delivery techniques through which the drug could be delivered more precisely and unaffectedly to the target tissue for producing better effect avoiding the unwanted effects at maximum level. Liposome, phytosome, ethnosome, nanoparticles, implants, transferosomes, microemulsions, proniosomes, etc. are some newer drug delivery systems that have been found very much promising for delivering different kinds of plant extracts quite successfully by oral/topical route to overcome their poor bioavailability in conventional formulations. Among these, liposomes are useful for delivering both hydrophilic and hydrophobic drugs, whereas phytosomes are designed for delivering hydrophilic drug molecules mostly. For the topical application ethnosomes, transferosomes and microemulsions show a great promise. In the better stability purpose and controlled/site specific delivery of drugs nanoparticles and proniosomes provide a superior standard. For a long time, traditional medicines were not considered for novel formulations due to the insufficiency on the scientific justification and processing difficulties, like extraction, identification, proper time selection, standardization, etc. But modern research evolving the way of newer formulations with the plant extracts and actives put forward an enthusiastic aspect for the healthcare system with the traditional medicine. Especially the critical traditional drugs like anticancer, antiasthmatic, antihypertensive, antidiabetic, etc., have a great future to be explored under the newer drug delivery systems. Keywords: Traditional medicine; drug delivery; nanoparticles; bioavailability. 23 International Conference Updates on Natural Products in Medicine and Healthcare Systems Invited Lecture Macrophage-derived protein kinase C-δ is a key molecule for pancreatic β-cell destruction in streptozotocin-induced diabetes. Asish Kumar Das1, Hiroyuki Mizuguchi2, Masashi Hattori2, Yoshiaki Kitamura3, Noriaki Takeda3, Hiroyuki Fukui2 1 Pharmacy Discipline, Khulna University, Khulna, Bangladesh. 2 Department of Molecular Pharmacology and 3Otolaryngology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan. Abstract Accumulating evidence suggests that protein kinase C-δ (PKC-δ) is involved in pancreatic -cell destruction in diabetes, in which activation of PKC-δ expressing in -cells up-regulates proinflammatory cytokines including IL-1 , TNF-α, and IFN- , and these cytokines induce apoptosis in -cells. However, little is known about the mechanism underlying this. Recently, we demonstrated that expression level of histamine H1 receptor (H1R) gene affects the severity of allergy symptoms. We also showed the involvement of PKC-δ signaling in the H1R upregulation. We explored natural resources for anti-allergic compounds that suppress H1R upregulation and identified some compounds including quercetin. Quercetin inhibited PKCδ signaling by the inhibition of PKCδ Tyr 311 phosphorylation, which is necessary for enzyme activation. These findings led us to the hypothesis that quercetin is useful to alleviate diabetic symptoms. In this study, we investigated the effect of quercetin on -cell destruction in streptozotocin (STZ)-induced diabetic model mice. Oral administration of quercetin (50 mg/kg) alleviated STZ-induced diabetic symptoms. Quercetin treatment suppressed -cell destruction caused by STZ. PKCδ positive macrophages were infiltrated into pancreatic islet in response to STZ treatment. A PKCδ inhibitor, rottlerin suppressed phorbol-12-myristate-13-acetate-induced up-regulation of IL-1 expression in peritoneal macrophages. In conclusion, we have demonstrated that treatment with STZ causes infiltrationof PKC฀-positive macrophages into pancreatic islet and activation of PKCδ signaling in macrophages increases cytokine expression and causes apoptosis in -cells. Quercetin alleviated diabetic symptoms by suppression of cytokine expression through the inhibiton of PKCδ signaling. Our data suggest that macrophage-derived PKCδ is the key molecule for pancreatic -cell destruction in STZ-induced diabetes and this finding may contribute to the clinical application of the compounds targeted for PKCδ signaling as new therapeutic agents for diabetes. Keywords: Diabetes; protein kinase C-δ; anti-allergic compounds; pancreatic streptozotocin-induced diabetes. 24 International Conference Updates on Natural Products in Medicine and Healthcare Systems -cell; Oral Presentation In vitro thrombolytic effects of roots of four medicinal plants available in Bangladesh. Fahad Hussain*1, Md. Ariful Islam1, Latifa Bulbul1, Md. Mizanur Rahman Moghal1, Mohammad Salim Hossain1 1 Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali 3814, Bangladesh. Abstract Thrombus formation inside the blood vessels obstructs blood flow through the circulatory system leading hypertension, stroke to the heart, anoxia and so on. Thrombolytic drugs are widely used for the management of cerebral venous sinus thrombosis patients but all of these have certain limitations. Medicinal plants and their components possessing antithrombotic activity have been reported before; however, plants that could be used for thrombolysis has not been reported so far. This study's aim was to evaluate the thrombolytic potential of selected plants‟ root extracts. An in vitro thrombolytic model was used to check the clot lysis effect of four methanol soluble roots extracts viz., Acacia nilotica, Justicia adhatoda, Azadirachta indica and Lagerstroemia speciosa along with Streptokinase as a positive control and water as a negative control. The roots extracts of A. nilotica, L. speciosa, A. indica, and J. adhatoda showed 15.1%, 15.49%, 21.26% and 19.63% clot lysis respectively at 5mg extract/ml NaCl solution concentration. The reference Streptokinase showed 47.21% and 24.73% clot lysis for 30,000 I.U. and 15,000 I.U. concentrations, respectively where 0.9% normal saline showed 5.35% clot lysis. The selected plants‟ roots extracts possess marked thrombolytic properties that could lyse blood clots in vitro; however, in vivo clot dissolving properties and active components responsible for clot lysis are yet to be discovered. Keywords: Thrombolytic potential, Acacia nilotica, Justicia adhatoda, Azadirachta indica, Lagerstroemia speciosa. 25 International Conference Updates on Natural Products in Medicine and Healthcare Systems Oral Presentation Berberine loaded nanoparticles: an implication for antimicrobial therapy Md. Harun Al Rashid1, Vivekananda Mandal2, Subhash C Mandal1, S. K. Mishra3, S. Sahoo3 and S.K. Sahoo4 1 Pharmacognosy and Phytotherapy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700032, India. 2 Department of Pharmacognosy, School of Pharmaceutical Sciences, Guru Ghasidas Central University, Bilaspur-495009, India. 3 University Department of Pharmaceutical Sciences (UDPS), Utkal University, Bhubaneswar751004, India. 4 Institute of Life Sciences, Nalco Square, Bhubaneswar-751023, India. Abstract Berberine is the isoquinoline alkaloid, chiefly obtained from Berberis aristata DC (familyBerberidaceae). It is reported to possess anticancer, antidiarrhoeal, anti-inflammatory, antidiabetic, hepatoprotective, antimicrobial and antioxidant activities. The main objective of this work is to evaluate the antimicrobial activity of berberine and its nanoparticles. In the present investigation, berberine was isolated from the stem of B. aristata. The isolated berberine was purified and characterized. Berberine loaded nanoparticles were prepared by GMO-base polymeric method and then characterized. The berberines as well as its nanoparticles were screened for their possible antimicrobial activity against selected bacterial and fungal strains by disc diffusion assay method. The results of antimicrobial screening revealed that the berberine and its nanoparticles are endowed with promising anticancer and antimicrobial properties. In most of the cases, the nanoparticles of berberine exhibited higher antibacterial and antifungal activities than the native berberine. Therefore, it is concluded that the berberine and its nanoparticles are potent antimicrobial agents against the selected bacterial and fungal strains. The development of nanoparticles of berberine is quite beneficial as evidenced from the enhancement of its antimicrobial activity. Keywords: Berberis aristata DC; Nanoparticles; Antibacterial & Antifungal activity. 26 International Conference Updates on Natural Products in Medicine and Healthcare Systems Oral Presentation Alpha amylase enzyme inhibitory and anti-inflammatory effect of Lawsonia inermis Hasan Imam1*, Nasir Uddin Mahbub1, Md. Forhad Khan1, Humayera Kabir Hana1, and Md. Moklesur Rahman Sarker1 1 Department of Pharmacy, School of Science, Primeasia University, HBR Tower, Kamal Ataturk Avenue, 9 Banani C/A, Dhaka- 1213, Bangladesh. Abstract Inflammatory pain is commonly associated to diabetic patients. Postprandial glucose level reduction by inhibiting the α-amylase, an enzyme relating to carbohydrate metabolism, is a realistic approach in diabetic patient. We focused two things in our study, one was invitro αamylase inhibition and another was reduction of inflammatory pain in mouse by the methanolic leaf extracts of Lawsonia inermis. The leaves were collected, identified and methanolic extracts were prepared. Assay was done by following the chromogenic DNSA method, and enzymatic activity was calculated contracting a maltose standard curve. Acarbose (10µg/ml) was used as a positive control. Acetic acid induced writhing test is started by intra peritoneal administration of 0.6% acetic acid (0.45ml/mouse) 30 min later. It causes a painful inflammatory effect. The painful sensation of mouse, which was manifested as abdominal writhe, was counted after the acetic acid injection. Statistical analysis was performed using Microsoft Excel 2007 and data were compared with unpaired two tailed t-test where p value threshold was used ≤0.05 to indicate a statistical significant. L. inermis extracts showed 29.63; 53.28; 60.97; 48.12 and 57.83 % reduction of enzymatic activity at 5; 7.5; 10; 12.5 and 15µg/ml concentrations of L. inermis extract, respectively. Untreated one was considered as 100% enzymatic activity. Acarbose, standard drug for α-amylase enzyme inhibition, at 10µg/ml concentration, reduced the enzymatic activity by 76.30% compared to untreated sample. In acetic acid induced writhing test, showed 16.33,14, 11.66, 11 times mean writhing at the doses of 200, 300, 400, and 500 mg/kg of the extract, respectively. Diclofenac, as a positive control, that showed 5 mean writhing at a dose 20 mg/kg, where as 0.9% saline showed 20.33 times mean writhing. The LD50 value of the aqueous extract of L. inermis was found quite high, 894 mg/kg in mice. These pharmacological properties can attribute the folkloric use of the plant in the management and control of hyperglycemia and arthritic pain. Further investigation in human is essential to reveal its therapeutic potential. Keywords: Alpha amylase; Lawsonia inermis; anti-inflammatory effect; chromogenic DNSA method. 27 International Conference Updates on Natural Products in Medicine and Healthcare Systems Oral Presentation Jatropha gossypiifolia: a potential cardio protective plant Md. Mahmodul Islam1, Md. Ariful Islam1 1 Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali 3814, Bangladesh. Abstract This study was undertaken to apply standard pharmacological and phytochemical methods in order to establish scientific evidence of traditionally used medicinal plant‟s pharmacological use and isolate the active constituents that may offer a local natural source of a novel therapeutic agent. Coronary obstructive diseases having thrombotic obstruction at the artery are a major cause of death and disability. All available thrombolytic agents still suffer significant shortcomings, including large therapeutic doses and limited fibrin specificity. An improved efficacy and fibrin specificity of thrombolytic therapy are now on demand. Euphorbiaceae plants show variety of secondary metabolites including thrombolytic compounds. Therefore the aim of present study was to screen methanolic extract of J. gossypiifolia leaves for their clot lysis property by using an In Vitro clot lysis model. In thrombolytic activity investigation, streptokinase was used as standard and 0.9% NaCl as negative control. This study was approved by Noakhali Science and Technology University and regulated under the Medical Institution Section at September, 2012. With informed consent form, human blood, collected from volunteers (n = 10) at University Medical Institution, immediately transferred into pre-weighed microcentrifuge tube, mixed with CaCl2 and incubated at 37°C for 45 minutes. After clot formation, serum was removed and clot weight was determined. Plant extracts were added to tubes with clots and incubated at 37ºC for 90 minutes. Remaining fluid was removed to weight. The difference was expressed as % clot lysis as mean ± SEM. Statistical comparisons were made using one-way ANOVA with Dunnett-t test. Investigation shows that at 2.5mg/mL concentration, J. gossypiifolia exhibits highest thrombolytic activity (17.25%), where 20mg/mL showed the least (7.723%). Standard streptokinase showed 47.218% of clot lysis at 30,000I.U and 0.9% NaCl shows 5.353% of clot lysis. As the leaves of J. gossypiifolia, possess Glycosides, Tannins, Phytosterols and Phenols like phytochemical constituents. This authoritative investigation may have significant implications in cardiovascular health and may designate the possibility of developing novel thrombolytic compounds from J. gossypiifolia, as it contains various phytoconstituents. Further studies are afoot to isolate and characterize the responsible compounds for thrombolytic activity. Keywords: Jatropha gossypiifolia; thrombolytic; streptokinase; phytoconstituents. 28 International Conference Updates on Natural Products in Medicine and Healthcare Systems Oral Presentation Immunostimulating activity of Ayurvedic preparations Saribadi, Anantamool Salsa, Mrittunjoy and Saribadyarista in vitro Md. Moklesur Rahman Sarker1, 2*, and M Shahabuddin Kabir Choudhuri3 1 Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Tsushima-naka, Kita-ku, Okayama 700-8530, Japan 2 Department of Pharmacy, Faculty of Science, Primeasia University, HBR Tower, 9 Banani C/A, Dhaka-1213, Bangladesh 3 Department of Pharmacy, Faculty of Biological Science, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh Abstract A healthy immune system by its self-surveillance mechanism prevents all sorts of infectious (bacterial, viral, fungal, etc.), and eliminate/destroy cancer cells as soon as they appear. The normal defensive capacity of the immune system is impaired by many factors and in many conditions, such as, diabetes, cancer, AIDS, stresses, chronic insomnia, aging, lack of exercise, poor nutrition, prolonged use of antibiotics, etc. Immunity is drastically fall in patients receiving chemotherapy and radiotherapy. Under those immune-compromised conditions external immunostimulants may play a significant role for the up-regulation of the immune system. The interest in herbal medicines is growing because of its low toxicity, no side-effects, and diversifying sources of new therapeutic agents. This study aimed to evaluate the immunostimulating potential of four traditional Ayurvedic preparations Saribadi (SDI), Anantamool Salsa (ANS), Mrittunjoy (MTJ) and Saribadyarista (SRT), by measuring IgM productions and splenocytes proliferations in vitro. BALB/c female mice whole splenocytes were treated with 0, 0.25, 0.5, 0.75, 1, 1.5, 2, 3, and 4% (v/v) of each preparation, and the cells were subcultured at 37°C for 120 hours. IgM productions in supernatants and proliferations of cells were determined by enzyme-linked immunosorbant assay (ELISA) and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenylterazolium bromide (MTT) assays, respectively. SDI, ANS, MTJ and SRT at a concentration of 0.25-1.50 % (v/v) significantly augmented IgM productions. The highest enhancement of IgM productions by SDI, ANS, MTJ and SRT were observed at the doses of 0.25, 1.50, 0.50 and 1.00%, respectively, which promoted IgM productions 3.76, 3.23, 3.29 and 4.12 times than that of control. Those preparations moderately stimulated the proliferations of cultured splenocytes as well. The IgM productions of those preparations were not retarded by the treatment of cells with polymixin B. Thus, the study demonstrates that the IgM production ability of the preparations was not due to the presence of bacterial endotoxin (LPS) rather owing to the active substance(s) in its preparations. Therefore, we can conclude that the Ayurved preparations Saribadi, Anantamool Salsa, Mrittunjoy and Saribadyarista may be useful in strengthening immune responses in case of insufficient or impaired immunity. Keywords: Immune response; bacterial endotoxin (LPS); Saribadi; Anantamool Salsa; Mrittunjoy; Saribadyarista. 29 International Conference Updates on Natural Products in Medicine and Healthcare Systems Oral Presentation Investigation on antidiabetic action of aqueous extract of Aloe vera on normal and Streptozotocin induced type 2 diabetic rats. Mehdi Bin Samad2*, Ashraf Ul Kabir1, Ninadh Malrina D‟Costa1, Mohammad Rajib Jahan2, Farjana Akther2, JMA Hannan1, 2 1 Department of Pharmacy Practice, North South University, Dhaka-1229, Bangladesh. 2 Department of Pharmaceutical Sciences, North South University, Dhaka-1229, Bangladesh. Abstract Diabetes is a widely prevalent endocrine disorder worldwide. In previous studies, Aloe vera has been shown to possess antidiabetic activity. Here, we aimed at discovering the underlying mechanism of its antidiabetic action. The study was carried out in accordance to recommendations of the “International Association for the study of Pain”. To evaluate the ability of Aloe Vera to lower acute glucose load and basal glucose we carried out the “Glucose tolerance test”, “Fasting Blood Glucose” respectively. The effect of sucrose absorption from various segments of the gut (the stomach, the upper 20 cm, middle, and lower 20 cm of the small intestine, the cecum, and the large intestine.) was determined by “Six Segment Test”. An “Intestinal Perfusion” technique was used to study the effect of Aloe vera on intestinal absorption of glucose in 36 h fasted non-diabetic rats. Finally, alteration of “GUT motility” was determined to see the duration food content would be in the intestines for sugar in food to be absorbed. Groups of data were compared using unpaired Student‟s t test and Mann–Whitney U test where appropriate. Where data were collected over a number of time-points, analysis was based on repeated measures ANOVA, with Bonferroni adjustment. . One-way ANOVA was performed and pair-wise comparisons to the control group made using Dunnett‟s test. Differences were considered significant at P<0·05. Administration of Aloe vera extract (0·5 g/kg) to normal and type 2 diabetic rats significantly (P<0.05) improved oral glucose tolerance. It also lowered the basal glucose level in the rats. Total remaining unabsorbed sucrose in the gastrointestinal tract of non-diabetic and type 2 diabetic rats, following oral sucrose loading (2·5 g/kg body weight) was not changed significantly by Aloe vera. However, the extract significantly (P<0.05) suppressed the intestinal glucose absorption in both non-diabetic and type 2 diabetic rats. The extract also increased (P<0.05) gastrointestinal motility in non-diabetic rats. The present findings indicate that the Aloe vera exerts hypoglycemic effects mediated through inhibition of carbohydrate absorption. This was achieved by reduction of sugar absorption and by increased GUT motility. Key Words: Intestinal Perfusion, Six Segment Test, Gut Motility, Antidiabetic, Aloe Vera. 30 International Conference Updates on Natural Products in Medicine and Healthcare Systems Oral Presentation Prostaglandin J2; Natural Bioactive Lipid Modules Adipocyte Inflanmmation Mohammad Salim Hossain1,*, Abu Asad Chowdhury2, Mitsuo Jisaca3, Tsutomu Nagaya3, Kazushige Yokota3 1 Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali3814, Bnagladesh. 2 Department of Pharmaceutical Chemistry, Dhaka University, Dhaka-1000, Bangladesh. 3 Department of Life Science and Biotechnology, Shimane University, 1060 Nishikawatsu-cho, Matsue, Shimane 690-8504, Japan. Abstract Obesity includes the changes in the functions of adipocytes, such as the onset of insulin resistance and adipocyte inflammation. Involving the arachidonate-cyclooxygenase (COX) pathway, prostaglandin (PG) D2 can be produced in adipocytes and dehydrated to J2 series of PGs including 15-deoxy-12,14-PGJ2 (15d-PGJ2) and 12-PGJ2, which serve as pro-adipogenic prostanoids through the activation of PPAR. Macrophage infiltration into adipose tissue increases with obesity, a condition associated with low-grade inflammation and insulin resistance. Monocyte chemoattractant protein-1 (MCP-1) is known as an inflammatory cytokines. However, regulation of MCP-1 expression through COX pathway during the life stage of adipocytes has not been studied well. Here we studied the role of COX inhibitors in adipogenesis and also aimed at the investigation of MCP-1 expression during adipogenesis with a vision to explore whether the J2 series prostaglandins have pervasive effect on expression of proinflammatory cytokines in cultured adipocytes. 3T3-L1 cells were grown, differentiated and maturated in suitable culture media along with or without different treatment ( Aspirin, prostaglandins). the end of maturation of adipocytes, total RNA was extracted and analyzed for the expression of adipgenic genes. Our results revealed that COX inhibitors negatively regulate the adipogenesis in cultured adipocytes. MCP-1 was highly expressed during the maturation phase of adipogenesis. Furthermore, expression of MCP-1 was regulated by the treatment with either of 12-PGJ2 or 15d-PGJ2. Although 12-PGJ2 was slightly less potent than 15d-PGJ2, each of these PGJ2 series rescued effectively the gene expression of MCP-1 that was attenuated in the presence of aspirin. Our findings indicate that adipocyte inflammation is regulated by MCP-1 expression through the COX pathway in cultured adipocytes. Keywords: 3T3-L1 cells; adipogenesis; monocyte chemoattractant protein-1; prostaglandin J2 series. 31 International Conference Updates on Natural Products in Medicine and Healthcare Systems Oral Presentation A comprehensive ethno-pharmacological and phytochemical update review on Terminalia arjuna. Fatema Binte Hafiz1, Nayeem Md. Towfique1, Monokesh Kumer Sen1, S. N. Sima2 and M Mizanur Rahman1‫٭‬ 1 Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh. 2 Department of Botany, Rajshahi University, Rajshahi, Bangladesh. Abstract To summarize the ethno-pharmacological uses and phytocemical constituents of Terminalia arjuna belongs to the family of Combretaceae and used in various indigenous system of medicine of Bangladesh. This review has been conducted to pile up information that is available in different scientific literatures. From the results, it reveals that wide numbers of phytochemical constituents have been isolated from the plant e.g. arjunin, arjunetin, gallic acid, terminic acid, pyrocatechols, luteolin, -Sitosterol, calcium, magnesium, zinc, copper and these possess multiple pharmacological and medicinal activities such as anti-mutagenic, anti-bacterial, antiviral, anti-oxidant, anti-inflammatory, anti-atherosclerotic, anti-diabetic etc. and various other important medicinal properties. The present comprehensive update review is therefore, an effort to give detailed information on phytochemical and pharmacological studies of T. arjuna and this work stimulates the researchers for further research on the potential use of this medicinal plants having pharmaceutical potential. Keywords: Terminalia arjuna; phytochemical; ethno-pharmacological; scientific literatures. 32 International Conference Updates on Natural Products in Medicine and Healthcare Systems Oral Presentation An unexplored Treasure of Spirulina and Medicinal Herbs Zahid Hossain* Managing Director & CEO, ZAP NATURAL PRODUCT LTD, House No. 10(ga), Road No.2, Shamoli, Dhaka-1207, Bangladesh. email: zahid.hossain1@gmail.com Abstract Zap Natural Product Ltd. (ZAP) is a major producer, processor and exporter of Spirulina and Medicinal Herbs in Bangladesh. The company is keen to develop an enduring relation with reputed herbal Medicine and Nutraceuticals companies as their trusted and consistent raw material and semi finished custom made product supplier. The company also offers the possibility of adopting and modifying industrial requirements in terms of quality assurance for the provision of the best natural ingredients for further processing and manufacturing. Keywords: ZAP; Natural ingredients; Spirulina; Medicinal Herbs. 33 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Preclinical blood chemistry safety profile studies of “Amlapittantaka Ras” after chronic administration to male Sprague-Dawley rats Unsuara Ferdous, Md Afaz Uddin*, Sarder Arifuzzaman, Ishtiaque Ahmed Chowdhury, Maksud Hassan and M. S. K. Choudhuri Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Abstract Amlapittantaka Ras (APT) is an Ayurvedic preparation used as a traditional medicine in the treatment of hyperacidity in the rural population. To find out the toxicological characteristic of APT, it was administered chronically to the male rats at a dose of 400 mg/kg. After 27 days of chronic administration of the APT preparation the following toxicological changes were noted. In the male rats there was decrease in the Total protein (5.19 % decr.) content in the plasma which was not significantly (p=0.372) different from their corresponding control values. In the male rats there was negligible decrease in the triglyceride level (4.84 % decr.) (p=0.916) and VLDL (4.84 % decr.) (p=0.916). Whereas negligible increase in the total cholesterol (2.32 % incr.) (p=0.615) and LDL (4.57 % incr.) (p=0.621) was noted. Though not statistically significant (p=0.151) yet prominent increase was noted in the case of HDL (48.34 % incr.) content in the plasma. None of the changes were statistically significantly different from their corresponding control values. The decrease in Total cholesterol /HDL ratio (19.13 % decr.) (p=0.390) and the decrease in LDL/HDL ratio (28.33 % decr.) (p=0.206) were not statistically significantly different from their corresponding control values. APT increased the bilirubin level (29.20% incr.) which was not statistically significant (p=0.661). There was an increase in the creatinine (60.60% incr.) content in plasma in the APT treated male rats which was not statistically significant yet it was noticeable (p=0.089). On the contrary, a decrease of urea level (19.09% decr.) in the plasma was noted in comparison to their control group, the decrease was not statistically significant though it was noticeable too (p=0.094). The increase in Urea / Creatinine ratio (113.41 % incr) was statistically significant (p=0.019). APT caused 9.67% decrease in plasma uric acid which was not statistically significant (p=0.720). Keywords: Ayurvedic medicine; Amlapittantaka Ras; Rat, Blood chemistry;Lipid profile; Liver function; Kidney function. 34 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Preclinical blood chemistry safety profile studies of “Echchabhedii Rosh” after chronic administration to male Sprague-Dawley rats Mohammod Jahidul Islam, Swagata Sarker*, Projjal Kanti Biswas, Tahmina Akter, Paritosh Chakma and M. S. K. Choudhuri Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Abstract Echchabhedii Rosh (EBR) is an Ayurvedic preparation used as a traditional medicine in the treatment of ascites in the rural population. To find out the toxicological characteristic of EBR, it was administered chronically to the male rats at a dose of 400 mg/kg. After 28 days of chronic administration of the EBR preparation the following toxicological changes were noted. In the study, the total protein content in the plasma was decreased (7.99 % decr.) in the EBR treated male rats. The decrease in total protein was not statistically significant yet it was noticeable (p=0.073). Interestingly, the albumin content was increased (14.87 % incr.) and the globulin content was decreased (29.05 % decr.) though not significantly in both the cases (respectively p=0.483 and p=0.410) in EBR treated male rats. In the male rats there was all throughout decrease in the triglyceride level (47.00 % decr.), total cholesterol (24.03 % decr.), VLDL (52.11 % decr.) and LDL (28.17 % decr.). The only exception was an increase in HDL (239.05 % incr.) content in the plasma. After chronic administration of EBR the triglyceride level was 47.00 % (p=0.229) decreased in male rats group which was not statistically significant. In this investigation statistically significant (p=0.016) decrease of plasma total cholesterol level in the EBR treated male rats was observed in comparison to control. Also a decrease in the VLDL and LDL content in the plasma was noted. Statistically insignificant decreases were observed in case of VLDL (52.11% decr.) (p=0.157) and though not significant yet noticeable decrease was observed in case of LDL (28.17% decr.) (p=0.054) from their corresponding control, whereas 239.05% increase of HDL level was noticed though which was not statistically significant (p=0.259). After chronic administration of Echchabhedii Rosh (EBR) to the male rats an increase of bilirubin level (280.0 % incr.) in the plasma was noted in comparison to their control group which was not statistically significant but yet it was noticeable (p=0.055). Also a statistically insignificant though noticeable decrease in the urea (21.34% decr.) content in plasma was noted (p=0.077). EBR treated male rats demonstrated 50.60 % increase in plasma uric acid content which was not statistically significant (p=0.102) though it was noticeable. In the male rats there was a statistically insignificant 14.87 % increase in the sGPT (p=0.483) and also a statistically insignificant 10.05 % decrease in the sGOT activity in the plasma (p=0.685). Keywords: Ayurvedic medicine; Echchabhedii Rosh;Rat; Blood chemistry; Lipid profile; Liver function; Kidney function. 35 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Preclinical blood chemistry safety profile studies of “Chaturmukh Ras” after chronic administration to male Sprague-Dawley rats Sarmin Jahan1, Nayma Abedin*1, Shemonty Hasan Punnyah1, Ferdousi Akter1, Abdullah Hel Kafee2 and M. S. K. Choudhuri1 1 Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. 2 Department of Pharmacy. North South University, Dhaka, Bangladesh. Abstract Chaturmukh Ras (CTM) is an Ayurvedic preparation used as a traditional medicine in the treatment of epilepsy in the rural population. To find out the toxicological characteristic of CTM, it was administered chronically to the male rats at a dose of 400 mg/kg. After 27 days of chronic administration of the CTM preparation the following toxicological changes were noted. In the male rats there was decrease in the Total protein (9.23 % decr.) content in the plasma which was though not statistically significantly different from their corresponding control values yet it was noticeable (p=0.068). In the male rats there was statistically significant decrease in the total cholesterol (18.85 % decr.) (p=0.013) and in the case of LDL (31.04 % decr.) though the decrease was not statistically significant, yet it was noticeable (p=0.097). Whereas increase was noted in the triglyceride level (4.96 % incr.) (p=0.919), VLDL (23.13 % incr.) (p=0.618) and HDL (81.09 % incr.) (p=0.194) content in the plasma. None of the increase in the triglyceride level, VLDL and HDL content were significantly different from their corresponding control values. The decrease in Total cholesterol /HDL ratio (69.67 % decr) was statistically significant (p=0.020) but the decrease in LDL/HDL ratio (80.69 % decr.) though not statistically significant yet it was noticeable (p=0.061). CTM increased bilirubin level (89.20% incr.) in the plasma which was not statistically significant (p=0.399). There was increase in the creatinine (23.97% incr.) (p=0.518) content in plasma in the CTM treated male rats. On the contrary, a negligible decrease of urea level (1.04% decr.) (p=0.920) in the plasma was noted in comparison to their control group. Overall an increase in Urea / Creatinine ratio (36.59 % incr) (p=0.395) was noted. None of these changes were statistically significantly different from their corresponding control values. CTM did not reveal any change in the plasma uric acid content. Keywords: Ayurvedic medicine; Chaturmukh Ras; Rat; Blood chemistry; Lipid profile; Liver function; Kidney function. 36 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Preclinical blood chemistry safety profile studies of “Naradiya Mahalaksmi Bilas” after chronic administration to male Sprague-Dawley rats S J Sarah Muneem, Md Mahmudur Rahman Mamun*, Musfira Musfi, Pritam Saha Podder, Masnun Ali Antora and M. S. K. Choudhuri Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Abstract Naradiya Mahalaksmi Bilas (NMB) which is an Ayurvedic preparation is used as „traditional medicine‟ in the treatment of „sinusitis‟ amongst the rural population in India, Bangladesh, Srilanka, Nepal, Bhutan and Pakistan. With an objective to examine the toxicological characteristic of NMB, it was administered chronically up to 43 days to the male rats at a dose of 400 mg/kg. After 43 days of chronic administration of the NMB preparation to the male rats the following toxicological changes were noted. The study shows the Total Protein percentage (0.04 % incr.) in the NMB treated male rats ,which indicates that there was almost no change in the Total Protein. The albumin content was very highly significantly (p=0.001) decreased (34.33 %) and the globulin content was very highly significantly (p=0.001) increased (35.91 %) in NMB treated male rats. The decrease (50.76%) in the Albumin / Globulin ratio was statistically very highly significantly different from their corresponding control values (p=0.001). There was all over decrease in the triglyceride level (20.00 % decr.), total cholesterol (4.51 % decr.), LDL (81.06 % decr.) and HDL (33.13 % decr.) in the NMB treated male rats. Increase in plasma VLDL (90.48 % incr.) level was only an exceptional occurrence. Chronic administration of NMB decreased the triglyceride level by 20.00 % in male rats group which was statistically insignificant (p=0.349). At the same time, a decrease in the LDL and HDL content in the plasma was also noted. Significant decrease were observed in case of LDL (81.06% decr.) (p=0.014) and statistically insignificant yet noticeable (p=0.088) decrease were observed in case of HDL (33.13% decr.) from their corresponding control values , whereas 90.48% increase of VLDL level was noticed, which was not statistically significant (p=0.164). The increase in Total cholesterol /HDL ratio (81.64 % incr.), though, not statistically significant yet it was noticeable (p=0.087) and the decrease in LDL/HDL ratio (56.19 % decr.) was not statistically significant (p=0.248). A highly significant increase in the plasma urea level (23.64%) was noted (p=0.001). The increase in Urea/Creatinine ratio (26.91 % incr.) was statistically insignificant but noticeable (p=0.104). Keywords: Ayurvedic medicine; Naradiya Mahalaksmi Bilas; Rat; Blood chemistry; Lipid profile; Liver function ; Kidney function. 37 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Preclinical blood chemistry safety profile studies of “Pradarantak Ras” after chronic administration to male Sprague-Dawley rats Ishtiaque Ahmed Chowdhury*1, Sabrina Mahjabeen2, Rubaiyat-E-Tasneem Islam2, Soniya Akter1, Muslima Khanam1 and M. S. K. Choudhuri1 1 Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. 2 Department of Pharmacy, North South University, Dhaka, Bangladesh. Abstract Pradarantak Ras (PDR) is an Ayurvedic preparation used as a traditional medicine in the treatment of leucorrhoea in the rural population. To find out the toxicological characteristic of PDR, it was administered chronically to the male rats at a dose of 400 mg/kg. After 43 days of chronic administration of the PDR preparation the following toxicological changes were noted. In the study, there was almost no change in the Total protein (0.34 % decr.) in the PDR treated male rats. Obviously the decrease in the Total protein (p=0.938) was not significantly different from their corresponding control values. Interestingly, the albumin content was significantly (p=0.037) decreased (13.92 %) and the globulin content was insignificantly (p=0.577) increased (5.18 %) in PDR treated male rats. The decrease (13.48%) in the Albumin / Globulin ratio was not significantly different from their corresponding control values (p=0.373). In the male rats there was all throughout decrease in the triglyceride level (87.21 % decr.), total cholesterol (22.57 % decr.), VLDL (87.93 % decr.), LDL (28.48 % decr.) and HDL (6.30 % decr.) content in the plasma. After chronic administration of PDR the triglyceride level was 87.21 % (p=0.001) decreased in male rats group which was statistically very highly significant. In this investigation though not statistically significant yet noticeable (p=0.082) decrease of plasma total cholesterol level in the PDR treated male rats was observed in comparison to control. Also a decrease in the VLDL and LDL content in the plasma was noted. Very highly significant decrease were observed in case of VLDL (87.93% decr.) (p=0.001) and statistically insignificant decrease were observed in case of LDL (28.48% decr.) (p=0.211) from their corresponding control, whereas only 6.30% decrease of HDL level was noticed which was not statistically significant (p=0.701). The decrease in both Total cholesterol /HDL ratio (11.39 % decr.) (p=0.527) and in LDL/HDL ratio (45.84 % decr.) (p=0.833) was not statistically significantly different from their corresponding control values. After chronic administration of PDR to the male rats a statistically significant (p=0.032) decrease of bilirubin level (78.01% decr.) in the plasma was noted in comparison to their control group. There was an increase in the plasma creatinine (4.61%) in the PDR treated male rats, though this increase was not significant (p=0.658). On the contrary, a very highly significant increase in the urea (43.09%) content in plasma was noted (p=0.001). The increase in Urea / Creatinine ratio (57.09 % incr) was statistically very highly significant (p=0.001). Keywords: Ayurvedic medicine; Pradarantak Ra;, Rat; Blood chemistry; Lipid profile; Liver function; Kidney function. 38 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Preclinical blood chemistry safety profile studies of “Brihat Bangesvar Ras” after chronic administration to male Sprague-Dawley rats Tangila Afrin, M Rakib Hasan*, Naushad Abedin, Santosh Chaudhary, Md Al Foyjul Islam and M. S. K. Choudhuri Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Abstract Brihat Bangesvar Ras (BBG) is an Ayurvedic preparation used as a traditional medicine in the treatment of haematuria in the rural population. To find out the toxicological characteristic of BBG, it was administered chronically to the male rats at a dose of 400 mg/kg. After 27 days of chronic administration of the BBG preparation the following toxicological changes were noted. In the male rats there was decrease in the Total protein (10.04 % decr.) content in the plasma which was not significantly (p=0.278) different from their corresponding control values. In the male rats there was all throughout increase in the triglyceride level (359.64 % incr.), total cholesterol (3.93 % incr.), VLDL (178.30 % incr.) and HDL (5.11 % incr.). The only exception was a decrease in LDL (18.69 % decr.) content in the plasma. After chronic administration of BBG the triglyceride level was prominently (359.64 %) increased in male rats group, although it was not statistically significant (p=0.164). In this investigation also statistically insignificant (p=0.635) increase of plasma total cholesterol level in the BBG treated male rats was observed in comparison to control. Also an increase in the VLDL and HDL content in the plasma was noted. Insignificant increases were observed in both VLDL (178.30% incr.) (p=0.263) and HDL (5.11% incr.) (p=0.895) in comparison to their corresponding control, whereas 18.69% decrease of LDL level was noticed which was not statistically significant (p=0.185). The decrease in Total cholesterol /HDL ratio (25.31 % decr.) (p=0.346) and the decrease in LDL/HDL ratio (46.63 % decr.) (p=0.194) were not statistically significantly different from their corresponding control values. BBG decreased the bilirubin level (35.80% decr.) which was not statistically significant (p=0.377). There was increase in the creatinine (26.54% incr.) (p=0.484) content in plasma in the BBG treated male rats. On the contrary, a decrease of urea level (12.36% decr.) (p=0.336) in the plasma was noted in comparison to their control group. Overall an increase in Urea / Creatinine ratio (53.66 % incr) (p=0.216) was noted. BBG caused 26.34% decrease in plasma uric acid which was not statistically significant (p=0.248). Keywords: Ayurvedic medicine; Brihat Bangesvar Ras; Rat; Blood chemistry; Lipid profile; Liver function; Kidney function. 39 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Preclinical blood chemistry safety profile studies of “Sarivadi Vati” after chronic administration to male Sprague-Dawley rats Nushrat Zahan1, Fariha Tasnim*2, Mst Reshma Khatun1, Salma Ahmed1, Md Towhidul Islam Tarun1 and M. S. K. Choudhuri1 1 Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. 2 Department of Pharmacy, North South University, Dhaka, Bangladesh. Abstract Sarivadi Vati (SBD) is an Ayurvedic preparation used as a traditional medicine in the treatment of otitis media in the rural population. To find out the toxicological characteristic of SBD, it was administered chronically to the male rats at a dose of 400 mg/kg. After 43 days of chronic administration of the SBD preparation the following toxicological changes were noted. In the study, the total protein content in the plasma was increased (4.89 % incr.) in the SBD treated male rats. The increase in total protein was not significant (p=0.138). Interestingly, the albumin content was statistically highly significantly (p=0.003) decreased (16.94 % decr.) and the globulin content was highly significantly (p=0.006) increased (44.29 % incr.) in SBD treated male rats. The decrease (37.03%) in the Albumin /Globulin ratio was statistically highly significantly different from their corresponding control values (p=0.003). In the male rats there was all throughout decrease in the triglyceride level (7.95 % decr.), total cholesterol (21.03 % decr.), VLDL (7.95 % decr.) and LDL (44.30 % decr.), with the exception with HDL (7.63 % incr.) content in the plasma. After chronic administration of SBD the triglyceride level was 7.95 % (p=0.592) decreased in male rats group which was not statistically significant. In this investigation statistically significant (p=0.018) decrease (21.03 % decr.) of plasma total cholesterol level in the SBD treated male rats was observed in comparison to control. Statistically insignificant decrease was observed in case of VLDL (7.95% decr.) (p=0.592), on the contrary insignificant increase were observed in case of HDL (7.63% incr.) (p=0.638) from their corresponding control, whereas only 44.30% decrease of LDL level was noticed which though not statistically significant, yet it was noticeable (p=0.093). The decrease in Total cholesterol /HDL ratio (23.29 % decr.) (p=0.049) and the decrease in LDL/HDL ratio (30.63 % decr.) (p=0.015) were both statistically significantly different from their corresponding control values. After chronic administration of Sarivadi Vati (SBD) to the male rats a decrease of bilirubin level (32.75% decr.) in the plasma was noted in comparison to their control group which was not statistically significant (p=0.484). There was a statistically insignificant increase in the creatinine (4.76% incr.) (p=0.611) content in plasma in the SBD treated male rats. On the contrary, a statistically very highly significant (p=0.001) decrease of urea level (39.52% decr.) in the plasma was noted in comparison to their control group. The decrease in Urea / Creatinine ratio (46.28 % decr) was statistically significant (p=0.032). Keywords: Ayurvedic medicine; Sarivadi Vati; Rat; Blood chemistry; Lipid profile; Liver function; Kidney function. 40 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Preclinical blood chemistry safety profile studies of “Tamra Bhasma” after chronic administration to male Sprague-Dawley rats Paritosh Chakma1, Mohsina Mukti*2, Rubina Akhter1, Maksud Hassan1, Sudip Kumar Dam1 and M. S. K. Choudhuri1 1 Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. 2 Department of Pharmacy, North South University, Dhaka, Bangladesh. Abstract Tamra Bhasma (TMR), an widely used Ayurvedic preparation as a traditional medicine in the treatment of anemia in the rural population of bangladesh. In this study our objective is to find out the toxicological characteristic of TMR and it was administered chronically to the male rats at a dose of 400 mg/kg. After 46 days of chronic administration of the TMR preparation the following toxicological changes were noted. In the study, the total protein content in the plasma was decreased (16.37 %) in the TMR treated male rats. The decrease in total protein was statistically highly significant (p=0.004). On the contrary, the albumin content was significantly (p=0.026) increased (41.74 %) in TMR treated male rats. In the male rats though there was a negligible decrease noted in the triglyceride level (1.67 % decr.) (p=0.964) and prominent decrease in the VLDL (34.45 % decr.) (p=0.333) content in the plasma, the decreases in both of the cases were not statistically significant. Whereas statistically highly significant increase in the total cholesterol (149.52 % incr.) (p=0.002), and in the case of LDL though not significant yet noticeable increase (58.11 % incr.) (p=0.094) was noted, besides in the case of HDL no statistical increase in HDL (14.66 % incr.) (p=0.435) content in the plasma was noted. The increase in Total cholesterol /HDL ratio (145.12 % incr) was statistically highly significant (p=0.007) and the increase in LDL/HDL ratio (72.08 % incr.) was statistically highly significant (p=0.008). After chronic administration of Tamra Bhasma (TMR) to the male rats an increase of bilirubin level (10.07% incr.) in the plasma was noted in comparison to their control group which was not statistically significant (p=0.820). There was an decrease in the plasma creatinine (11.76% decr.) in the TMR treated male rats, and this decrease was not significant (p=0.216). TMR caused about 31.65% decrease in plasma uric acid which was statistically significant (p=0.032). On the other hand, there is a statistically highly significant increase in the sGOT activity (47.73% incr.) (p=0.005) and a statistically very highly significant increase in the sGPT activity (72.37% incr.) (p=0.001) in the plasma was noted. Whereas there was a decrease in the ALK-Pase activity (4.42% decr.) (p=0.717) which was not statistically significantly different from the control male rats. A statistically significant increase in the LDH activity (63.02% incr.) (p=0.021) in the plasma was noted. Keywords:Ayurvedic medicine; Tamra Bhasma; Rat; Blood chemistry; Lipid profile; Liver function; Kidney function. 41 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Preclinical blood chemistry safety profile studies of “Praval Bhasma” after chronic administration to male Sprague-Dawley rats Kamrun Nahar, Jannatul Fardous*, Nishat Afrose, Kaniz Fatema, Arjyabrata Sarker and M. S. K. Choudhuri Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Abstract Praval bhasma (PBL) is an Ayurvedic preparation used as a traditional medicine in the treatment of osteoporosis in the rural population. To find out the toxicological characteristic of PBL, it was administered chronically to the female rats at a dose of 400 mg/kg. After 52 days of chronic administration of the PBL preparation the following toxicological changes were noted. In the female rats there was increase in the Total protein (10.20 % incr.) and the Globulin (37.93 % incr.) content in the plasma. There was almost no change in the Albumin (1.36 % incr.). None of the increase in the Total protein (p=0.170), in the Albumin (p=0.881) and the Globulin (p=0.207) content were significantly different from their corresponding control values. The decrease (35.66%) in the Albumin / Globulin ratio was not significantly different from their corresponding control values (p=0.200). In the female rats there was all throughout increase in the triglyceride level (14.26 % incr.), total cholesterol (77.27 % incr.), VLDL (14.26 % incr.) and LDL (232.78 % incr.). After chronic administration of PBL the triglyceride level was 14.26 % (p=0.190) increased in female rats group which was not statistically significant. In this investigation statistically very highly significant (p=0.001) increase of plasma total cholesterol level in the PBL treated female rats was observed in comparison to control. Also an increase in the VLDL and LDL content in the plasma was noted. Statistically insignificant increase were observed in case of VLDL (14.26% incr.) (p=0.190) and highly significant increase were observed in case of LDL (232.78% incr.) (p=0.002) from their corresponding control. After chronic administration of Pravala Bhasma (PBL) to the female rats a increase of bilirubin level (27.53% incr.) in the plasma was noted in comparison to their control group which was not statistically significant (p=0.849). There was a decrease in the creatinine (46.50% decr.) content in plasma in the PBL treated female rats which was statistically highly significant (p=0.009). PBL caused about 512.91% increase in plasma uric acid which was statistically significant (p=0.038). In the female rats a noticeable increase in the sGOT activity (24.17% incr.) (p=0.079) and a noticeable increase in the sGPT activity (27.12% incr.) (p=0.066) in the plasma was noted though in both the cases they were not statistically significant but as mentioned before were noticeable in both of the cases. Whereas there was a statistically highly significant decrease in the ALK-Pase activity (28.45% decr.) (p=0.009) and a statistically very highly significant increase in the LDH activity (113.30% incr.) (p=0.001) in the plasma. Keywords: Ayurvedic medicine; Praval bhasma; Rat; Blood chemistry; Lipid profile; Liver function; Kidney function. 42 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Preclinical blood chemistry safety profile studies of “Banga Bhasma” after chronic administration to male Sprague-Dawley rats Sumon Kanti Chowdhury, Marjana Khalil*, Shuvagata Kahali, Md Asikur Rahman, Fanniha Islam Panthi and M. S. K. Choudhuri Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Abstract Banga Bhasma (BNG) is an Ayurvedic preparation used as a traditional medicine in the treatment of leucorrhoea in the rural population. To find out the toxicological characteristic of BNG, it was administered chronically to the female rats at a dose of 400 mg/kg. After 52 days of chronic administration of the BNG preparation the following toxicological changes were noted. In the study, the total protein content in the plasma was increased (3.88 %) in the BNG treated female rats. The increase in total protein was not significant (p=0.504). Interestingly, the albumin content was statistically insignificantly (p=0.346) increased (6.82 %) and the globulin content was insignificantly (p=0.318) decreased (59.02 %) in BNG treated female rats. The negligible (0.16%) increase in the Albumin / Globulin ratio was not significantly different from their corresponding control values (p=0.996). In the female rats there was all throughout increase in the triglyceride level (3.52 % incr.), total cholesterol (95.50 % incr.), VLDL (3.52 % incr.) and LDL (60.63 % incr.) content in the plasma. After chronic administration of BNG the triglyceride level was 3.52 % (p=0.631) increased in female rats group which was not statistically significant. In this investigation statistically very highly significant (p=0.001) increase of plasma total cholesterol level in the BNG treated female rats was observed in comparison to control. Also an increase in the VLDL and LDL content in the plasma was noted. Statistically insignificant increase was observed in both the cases of VLDL (3.52% incr.) (p=0.631) and LDL (60.63% incr.) (p=0.160) from their corresponding control. After chronic administration of BNG to the female rats an increase of bilirubin level (92.55% incr.) in the plasma was noted in comparison to their control group which was not statistically significant (p=0.354)There was a negligible decrease in the plasma creatinine (1.49%) in the BNG treated female rats, and this decrease was not significant (p=0.865). BNG caused 355.92% increase in plasma uric acid which was statistically very highly significant (p=0.001). In the female rats a negligible increase in the sGOT activity (0.66% incr.) (p=0.946) and a prominent increase in the sGPT activity (20.54% incr.) (p=0.162) in the plasma was noted though in both the cases they were statistically insignificant. Whereas there was a statistically significant decrease in the ALK-Pase activity (23.62% decr.) (p=0.023) and a statistically significant increase in the LDH activity (38.15% incr.) (p=0.028) in the plasma. Keywords: Ayurvedic medicine; Banga Bhasma; Rat; Blood chemistry; Lipid profile; Liver function; Kidney function. 43 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Preclinical hormonal profile studies of “Tryodashanga Guggulu” after chronic administration to male Sprague-Dawley rats M. Babul Akhter*1, Maksud Hassan2, Marjana Khalil2, Naushad Abedin2, Musfira Musfi2 and M. S. K. Choudhuri2 1 Government Unani and Ayurveda Medical College, Mirpur , Dhaka , Bangladesh. 2 Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Abstract Tryodashanga Guggulu (TR-G), a classical Ayurvedic preparation which is used in musculoskeletal disorders, was studied for its effect on different hormonal parameters after chronic administrations for 45 days to male Sprague-Dawley rats. There is a statistically very highly significant (p=0.001) elevated serum total T3 (Triodothyronine) level. [ 650.0 % elevation]. There is an [4.95 %] elevation in the Free fraction of T3 (Triodothyronine) level, the elevation though not significant yet it was prominent (p=0.490). There is a [5.51 %] lowering in the seum Total T4 level, the lowering though not significant yet it was prominent (p=0.393). There is a [5.24 %] lowering in the serum Free fraction of T4 level, the lowering though not significant yet it was prominent (p=0.352). There is a statistically significant (p=0.037) lowering of the serum TSH level. [54.17 % lowering]. There is an [30.68 %] elevated serum Cortisol level, the elevation though not significant yet it was prominent (p=0.120). There is a negligible [0.88 %] elevated serum Testosterone level, which was statistically not at all significant (p=0.977). There is an [57.48 %] elevated serum Progesterone level, the elevation though not significant yet it was prominent (p=0.129). There is a negligible [1.31 %] decrease in the serum Estradiol level, which was statistically not at all significant (p=0.919). There is a [5.70 %] decrease in the serum FSH level, the decrease though not significant yet it was prominent (p=0.403). There is an [34.05 %] elevated serum LH level, the elevation though not significant yet it was prominent (p=0.210). There is a statistically significant (p=0.026) lowering of the serum Prolactin level. [18.21 % lowered]. Keywords: Ayurvedic medicine; Tryodashanga Guggulu; Rat; Hormone profile; Thyroid hormone; Sex hormone. 44 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation A Clinical Study on the Efficacy and Safety of “Panchatikta Ghrita Guggulu” Shurab Hossain*1, Nazrin Sultana1, M Rakib Hasan2, Nayma Abedin2, Swagata Sarker2 and M. S. K. Choudhuri2 1 Government Unani and Ayurveda Medical College, Mirpur, Dhaka, Bangladesh. 2 Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Abstract Panchatikta Ghrita Guggulu (PTK-G) an Ayurvedic preparation used as a traditional medicine in the treatment of skin diseases in the rural population and is included in Bangladesh National Ayurvedic formulary. A clinical study was conducted to find out the efficacy in eczema. PTK-G was administered orally at a dose of 500 mg capsules thrice daily for a period of 6 weeks in a single blind, quasi random clinical study comprising of 20 patients (6 males and 14 females). Hematological, biochemical and symptomatic evaluation were done. PTK-G reduced itching and vesicle formation. A total absence of scaling, oozing, erythema and fissure were noted. Haematological findings reveal lowering of ESR, decrease in total count (TC) of WBC with a noteworthy decrease in lymphocyte and eosinophil count ((p=0.015)). Clinical chemistry revealed that total cholesterol was decreased ↓1γ.05%, which was not significant but it was noticeable (p= 0.100). LDL and TG were noticeably decreased (↓15.1β% and ↓15.56%), though they were not statistically significant (p=0.177 and p= 0.237). Blood urea, creatinine, uric acid, sGOT, sGPT, blood sugar and serum alkaline phosphatase level were decreased. Lowering of hemoglobin level was noted as side effect. Increase in itching and oozing were noted after the initial 3 to 4 days of administration which was subsided after the 2 nd week and subsequently onwards. Caution should be taken in anemia, Diabetes mellitus and during co-administration of any anticoagulant, blood thinning, anti-diabetic drugs or lipid lowering agents as it may potentiate or deplete the activity of these drugs or herbs respectively. Keywords: Ayurvedic medicine; Panchatikta Ghrita Guggulu; Clinical study; Blood chemistry; Lipid profile; Liver function; Kidney function. 45 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Study on the effect of chronic administration of Jirakadyarista different hematologic parameters in male Sprague-Dawley rats Fazle Noor Biswas*1, Mohammad Ali2, Md Sabbir Zihad2, Mohammad Jashim Uddin2 and M. S. K. Choudhuri3 1 Ahmed2,Md Borhan Uddin Department of Pharmacy.,University of Development Alternative, Dhaka, Bangladesh. 2 Department of Pharmacy, Jessore Science and Technology University, Ambottala, Jessore, Bangladesh. 3 Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Abstract Jirakadyarista (JRK), a classical Ayurvedic preparation which is used in puerperal disorders, was studied for its effect on different hematologic parameters after chronic administrations for 54 days to male Sprague-Dawley rats. There is a statistically significant (p=0.025) increase in the absolute count of Neutrophils of the male rat. [57.67 % increase]. There is an [29.41%] decrease in the percentage of Monocyte count of the male rat, the decrease though not significant yet it was noticeable (p=0.053).There is a statistically significant (p=0.021) increase in the total numbers in the red blood cells of the male rat. [8.10 % increase]. There is an [1.92 %] increase in the Hemoglobin content of the blood of the male rat, the increase though not significant yet it was prominent (p=0.649).There is a statistically highly significant (p=0.009) increase in the Hematocrit level of the blood of the male rat. [8.36% increase]..There is a statistically highly significant (p=0.007) decrease in the Mean corpuscular hemoglobin, a red cell index of the male rat. [5.20 % decrease].There is a statistically very highly significant (p=0.01) decrease in the Mean corpuscular hemoglobin concentration, a red cell index of the male rat. [5.56 % decrease].There is a statistically highly significant (p=0.003) increase in the red cell volume distribution width, a red cell index of the male rat. [7.66 % increase]. There was no change noticed in Erythrocyte sedimentation rate in blood from the male rat.There is an [ 20.00 %] prolongation of male rat cutaneous tail bleeding time, the increase though not significant yet it was noticeable (p=0.082). There is a [7.19%] decrease in the Platecrit value of the blood of the male rat, the decrease though not significant yet it was noticeable (p=0.093). Keywords: Ayurvedic; hematology; Jirakadyarista. 46 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Study on the effect of chronic administration of Jambadyarista on different hematologic parameters in male Sprague-Dawley rats Sabrina Mahjabeen1, Maruf ul Islam*2, Md Muzibar Rahman2, Sheikh Shahriar Rajib2, Mohammad Jashim Uddin2 and M. S. K. Choudhuri3 1 Department of Pharmacy. North South University, Dhaka, Bangladesh. 2 Department of Pharmacy, Jessore Science and Technology University, Ambottala, Jessore, Bangladesh. 3 Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Abstract Jambadyarista (JMB), a classical Ayurvedic preparation which is used in diabetes, was studied for its effect on different hematologic parameters after chronic administrations for 45 days to male Sprague-Dawley rat. There is a statistically very highly significant (p=0.000) increase in the absolute count of Monocytes of the male rat. [60% increase].There is an [52.75%] increase in the percentage of Neutrophil count of the male rat, the increase though not significant yet it was noticeable (p=0.068).There is a statistically significant (p=0.035) decrease in the percentage of Lymphocyte count of the male rat. [7.92 % decrease]. There is a statistically highly significant (p=0.002) decrease in the Mean corpuscular volume, a red cell index of the male rat. [2.32% decrease]. There is an [2.67%] increase in the mean platelet volume of the male rat, the increase though not significant yet it was noticeable (p=0.095).There is an [10.62%] increase in the Platecrit value of the blood of the male rat, the increase though not significant yet it was prominent (p=0.123).There is a statistically highly significant (p=0.002) increase in the platelet volume distribution width of the male rat [2.39 % increase]. Keyword: Ayurvedic; hematology;Jambadyarista. 47 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Study on the effect of chronic administration of Saraswatarista on different hematologic parameters in male Sprague-Dawley rats Mehedi Rahman*1, Nahid Hasan1, Sourobh Paul1, Nahida Akhter1, IninTaznin1 and M. S. K. Choudhuri2 1 Department of Pharmacy. North South University, Dhaka, Bangladesh. 2 Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Abstract Saraswatarista (BRS), a classical Ayurvedic preparation which is used in mental disorders, was studied for its effect on different hematologic parameters after chronic administrations for 54 days to male Sprague-Dawley rats. There is an [33.67%] increase in the number of white blood cell count of the male rat, the increase though not significant yet it was noticeable (p=0.071).There is a statistically highly significant (p=0.008) increase in the absolute count of Neutrophils of the male rat. [89.21 % increase]. There is a statistically highly significant (p=0.01) increase in the percentage of Neutrophil count of the male rat. [40.46% increase]. There is a statistically highly significant (p=0.01) decrease in the percentage of Lymphocyte count of the male rat. [7.63% decrease]. There is a statistically highly significant (p=0.005) increase in the Mean corpuscular volume, a red cell index of the male rat. [2.03 % increase]. There is a statistically significant (p=0.032) decrease in the Mean corpuscular hemoglobin, a red cell index of the male rat. [3.46 % decrease].There is a statistically highly significant (p=0.003) decrease in the Mean corpuscular hemoglobin concentration, a red cell index of the male rat. [5.16 % decrease].There is a statistically very highly significant (p=0.000) increase in the red cell volume distribution width, a red cell index of the male rat. [15.7 % increase]. There is an [25.71%] prolongation of male rat cutaneous tail bleeding time, the increase though not significant yet it was noticeable (p=0.063). Keyword: Ayurvedic; hematology; Saraswatarista. 48 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Study on the effect of chronic administration of Debdarbadyarista on different hematologic parameters in male Sprague-Dawley rats Mohammad Nurul Islam Hawlader1, Md Nahinul Islam*2, Afsana Tajmim2, Md Mohiuddin Alamgir2, Mohammad Jashim Uddin2 and M. S. K. Choudhuri3 1 Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. 2 Department of Pharmacy, Jessore Science and Technology University, Ambottala, Jessore, Bangladesh. 3 Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Abstract Debdarbadyarista (DVD), a classical Ayurvedic preparation which is used in infectious diseases of diabetic subjects, was studied for its effect on different hematologic parameters after chronic administrations for 54 days to male Sprague-Dawley rats. There is a statistically significant (p=0.046) increase in the absolute count of Neutrophils of the male rat with [42.66 % increase]. There is a statistically significant (p=0.046) increase in the Mean corpuscular volume, a red cell index of the male rat [1.33 % increase]. There is a statistically significant (p=0.019) decrease in the Mean corpuscular hemoglobin, a red cell index of the male rat. [4.02% decrease].There is a statistically very highly significant (p=0.000) increase in the Mean corpuscular hemoglobin concentration, a red cell index of the male rat. [5.02 % decrease].There is a statistically significant (p=0.004) increase in Erythrocyte sedimentation rate in blood from the male rat. [12.29 % increase]. There is an [30.00 %] prolongation of male rat cutaneous tail bleeding time, the increase though less significant yet it was noticeable (p=0.060). There is an [7.02 %] prolongation of whole blood clotting time in male rats, the increase though not significant yet it was noticeable (p=0.074). Keyword: Ayurvedic; hematology; Debdarbadyarista. 49 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Study on the effect of chronic administration of Khadirarista on different hematologic parameters in male Sprague-Dawley rats Shahin Perves*1, Nahida Akhter1, Faika Laz Banti1, Bijoy Chokroborty2, Md. Mohabbulla Mohib1 and M. S. K. Choudhuri3 1 Department of Pharmacy, North South University, Dhaka, Bangladesh. 2 Department of Pharmacy, University of Development Alternatives, Dhaka, Bangladesh 3 Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Abstract Khadirarista (KDR), a classical Ayurvedic preparation which is used in dermatological disorders, was studied for its effect on different hematologic parameters after chronic administrations for 45 days to male Sprague-Dawley rats. There is an [63.75%] increase in the absolute count of Monocytes of the male rat, the increase though not significant yet it was noticeable (p=0.055). There is an [33.33%] increase in the percentage of Monocyte count of the male rat, the increase though not significant yet it was noticeable (p=0.053). There is a [3.55 %] decrease in the Hemoglobin content of the blood of the male rat, the decrease though not significant yet it was prominent (p=0.154).There is a statistically significant (p=0.016) decrease in the Hematocrit level of the blood of the male rat. [6.22% decrease]. There is a statistically significant (p=0.03) increase in the Mean corpuscular hemoglobin concentration, a red cell index of the male rat. [3.00 % increase]. There is a statistically significant (p=0.028) increase in the number of platelet count of the male rat. [15.12% increase]. There is an [3.51 %] increase in the mean platelet volume of the male rat, the increase though not significant yet it was noticeable (p=0.071).There is a statistically highly significant (p=0.003) increase in the Platecrit value of the blood of the male rat. [21.86 % increase]. There is a statistically very highly significant (p=0.0) increase in the platelet volume distribution width of the male rat. [3.31% increase]. Keyword: Ayurvedic; hematology; Khadirarista. 50 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Study on the effect of chronic administration of Basakarista different hematologic parameters in male Sprague-Dawley rats Abdullah Hel Kafee*1, Mehedi Rahman1, Inin Taznin1, Shahin Perves1, Mohsina Rahman Anee1 and M. S. K. Choudhuri2 1 Department of Pharmacy, North South University, Dhaka, Bangladesh. 2 Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Abstract Basakarista( BSK), a classical Ayurvedic preparation which is used in respiratory disorders, was studied for its effect on different hematologic parameters after chronic administrations for 54 days to male Sprague-Dawley rats. There is a statistically significant (p=0.023) increase in the number of white blood cell count of the male rat. [43.55 % increase]. There is a statistically highly significant (p=0.003) increase in the absolute count of Neutrophils of the male rat. [108.60% increase]. There is a statistically significant (p=0.020) increase in the percentage of Neutrophil count of the male rat. [44.82 % increase]. There is a statistically significant (p=0.038) decrease in the percentage of Lymphocyte count of the male rat. [7.63 % decrease].There is a statistically highly significant (p=0.002) decrease in the percentage of Monocyte count of the male rat. [52.94% decrease]. There is a statistically highly significant (p=0.004) decrease in the Mean corpuscular hemoglobin, a red cell index of the male rat. [3.76 % decrease].There is a statistically highly significant (p=0.002) decrease in the Mean corpuscular hemoglobin concentration, a red cell index of the male rat. [6.52 % decrease].There is a statistically highly significant (p=0.003) increase in the red cell volume distribution width, a red cell index of the male rat. [14.78% increase]. There is an [7.91%] increase in Erythrocyte sedimentation rate in blood from the male rat, the increase though not significant yet it was prominent (p=0.500).There is a statistically significant (p=0.037) prolongation of male rat cutaneous tail bleeding time [37.14 % increase]. Keyword: Ayurvedic; hematology; Basakarista. 51 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Study on the effect of chronic administration of Ashokarista on different hematologic parameters in male Sprague-Dawley rats Mohsina Mukti*1, Fariha Tasnim1, Abdullah Hel Kafee1, Tamara Towshin Alam1, Nahid Hasan1 and M. S. K. Choudhuri2 1 Department of Pharmacy. North South University, Dhaka, Bangladesh. 2 Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Abstract Ashokarista (ASK), a classical Ayurvedic preparation which is used in menstrual disorders, was studied for its effect on different hematologic parameters after chronic administrations for 54 days to male Sprague-Dawley rats. There is a statistically highly significant (p=0.01) increase in the number of white blood cell count of the male rat. [97.45% increase]. There is a statistically significant (p=0.02) increase in the absolute count of Neutrophils of the male rat. [293.42 % increase]. There is a statistically significant (p=0.05) increase in the absolute count of Lymphocytes of the male rat. [59.90% increase]. There is a statistically very highly significant (p=0.000) increase in the percentage of Neutrophil count of the male rat. [95.42% increase].There is a statistically very highly significant (p=0.000) decrease in the percentage of Lymphocyte count of the male rat. [18.20% decrease].There is a statistically significant (p=0.027) decrease in the percentage of Monocyte count of the male rat. [37.27 % decrease].There is a statistically significant (p=0.039) decrease in the total numbers in the red blood cells of the male rat. [26.05% decrease].There is a statistically highly significant (p=0.002) decrease in the Hemoglobin content of the blood of the male rat. [22.54 % decrease].There is a statistically significant (p=0.036) decrease in the Hematocrit level of the blood of the male rat. [20.77% decrease]. There is an [33.24%] increase in the red cell volume distribution width, a red cell index of the male rat, the increase though not significant yet it was noticeable (p=0.059).There is a statistically highly significant (p=0.008) increase in Erythrocyte sedimentation rate in blood from the male rat. [48.13 % increase]. There is a statistically very highly significant (p=0.000) prolongation of male rat cutaneous tail bleeding time. [66.67% increase].There is a statistically very highly significant (p=0.000) prolongation of whole blood clotting time in male rats [18.13 % increase]. Keyword: Ayurvedic; hematology; Ashokarista. 52 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Ethnomedicinal Plants Used by folk medicinal Healers in Tangail District, Bangladesh for treatment of different ailments Al Amin*1, Md. Nazmul Hasan1 , Md. Arif Khan1, Kalyan Ghatak2, Aysha Ferdoushi1, Md. Morsaline Billah2 , Farah Sabrin1 1 Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Santosh, Tangail- 1902, Bangladesh. 2 Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh. Abstract Villagers and suburban dwellers generally rely on folk medicinal healers (Kavirajes) to treat different ailments. Various ethnomedicinal plants are used for diverse ailments, which remain undocumented as well as undiscovered as a source of novel active pharmaceutical ingredients. Our survey points out to the conservation of ethnomedicinal and ethnobotanical knowledge, which is being lost day by day and subsequent scientific validation of this knowledge. The survey was conducted using a semi-structured questionnaire. The information was collected from fifteen folk medicinal healers of six villages of Tangail (24 ο 15 ̕ 00 ̕ ̕ N, 89ο 55 ̕ 00̕ ̕ E) district. This ethnobotanical survey was carried out from April 2012 to June 2013. SPSS statistics was used to analyze the obtained data. One hundred and seven medicinal plants were found to be used as cure for fifty two ailments. Among them, twenty four plants were used for treating intestinal disorders, thirteen for respiratory diseases, fifteen for fever and rheumatic pains, twelve for diabetes, five for jaundice, twelve for male and female sexual disorder and so on. The most frequently utilized plant parts were the leaf (37%), root (13%), fruit (17%), seed (10%), Stem (8%). The medicinal plant usages by the traditional healers revealed that many ailments were treated by a single plant while many plants were used for treating a single ailment. It was also found that these healers did not have proper knowledge of appropriate dosages for the treatment of a particular ailment and it varied from healers to healers. Apart from these, the healers were not keen to provide any information and therefore, the tendency of healers not to disclose information puts the risk of eroding the wealth of ethnomedicinal knowledge over time. Keywords: Ethnobotany; intestinal disorder; respiratory disease; rheumatic pain. 53 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Phytochemical and Pharmacological Investigation of Xanthium indicum (leaf) Antara Ghosh1, Farjana Afrin1, Md. Ariful Islam1, Mohammad Salim Hossain1*, Md. Mizanur Rahman Moghol1 1 Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali3814, Bangladesh. Abstract Xanthium indicum (Koenig) belonging to the family Asteraceae, and has been used as flock medicine for many years. This study has been designed to investigate for preliminary Phytochemical screening and evaluation of their Pharmacological activities in various pharmacological models. like anthelmintic activity, brine shrimp lethality bioassay, in-vitro thrombolytic activity and antioxidant activity. The crude methanolic extract was screened for different phytochemicals. Then the crude extract is investigated for the pharmacological activity. Crude methanolic extract was administered to different model to screen the pharmacological activities. The anthelmintic assay was done which displayed significant activity against the Phertima prosthuma .The experiment showed very significant result compare with standard( Piperazine citrate). A preliminary toxicological investigation of methanolic extract performed by brine shrimp bioassay technique. The LC50 value of crude extract showed moderate result (1.345µg/ml) comparison with positive control vincristine sulphate (with LC 50 0.839 µg/ml). In evaluation of in vitro thrombolytic activity of methaolic crude extract through in-vitro clot lysis model, the extract induced slight (p> 0.05) clot lysis of human blood with 17. 52% at 2.5 mg/ml, 13.19 % at 5mg/mL ; 11.58% at10mg/mL and 9.11 % at 20 mg/ml compare to control where the standard thrombolytic drug Streptokinase (30,000 IU and 15000 IU) evoked a significant clot lysis activity 47.22 % and 24.73% clot lysis respectively. The antioxidant activity of the crude methanolic extract was observed by determining total phenolic content and1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays. Result calculated as slight in methanolic crude extract 19.75 µg/mg of gallic acid equivalent and shows very poor scavenging activity. Key words: Xanthium indicum,anthelmintic, Brine shrimp lethality. Thrombolytic, Phertima prosthuma, Antioxidant 54 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Development of a cost effective microwave extraction process to assess the factor-response relationship buried in the experimental data collected from the extraction process of potent bioactives by soft computing techniques Anup Kumar Das1, Vivekananda Mandal2, Subhash C. Mandal1* 1 Pharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India. 2 Institute of Pharmacy, Guru Ghasidas Central University, Bilaspur 495009, India. Extraction forms the very basic step in natural product drug discovery research. A poorly optimized and planned extraction methodology can jeopardize the entire mission. To provide a vivid picture of different chemometric tools and planning for process optimization and method development in extraction of botanicals with emphasis on microwave assisted extraction (MAE) of botanicals. Studies involving the applications of chemometric tools in combination with MAE of botanicals in presented here. While attempting to discover the significant extraction factors and then optimizing a response by fine tuning those factors, experimental design or statistical design of experiment (DoE) which is a core area of study in chemometrics has been used for statistical analysis and interpretations. In this poster, a brief explanation of the different aspects and methodologies related to MAE of botanicals that have been subjected to experimental design are reviewed here in particular along with some general chemometric tools and the steps involved in its practice is presented. A detailed study on various factors and responses involved during the optimization is also presented. It will ultrimately assist to get a better insight into the chemometric strategies of process optimization and method development which will in turn improve the decision making process in selecting influential extraction parameters. Key words: Microwave extraction; chemometrics; soft computing; method development; bioactives. 55 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Ethnomedicinal Survey of Plants for Diabetes Mellitus Care in Tangail District, Bangladesh Arif Khan1, Al Amin1, Nazmul Hasan1, Aysha Ferdoushi1, Morsaline Billah2, Farah Sabrin1* 1 Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh. 2 Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh. Abstract Ethnobotany is the scientific study of human interactions with plants and environment. This includes the study how traditional and modern cultures across the globe interact or have interacted with plants. The incidence of Diabetes is growing rapidly in many countries in the world and very common in developing countries like Bangladesh. Around 180 million people are afflicted with diabetes worldwide and the prevalence is expected to more than double by the year in 2030. Diabetes is a heterogeneous group of syndromes characterized by an elevation of blood glucose level caused by a relative or absolute deficiency of insulin. Diabetes can cause serious hyperglycemia if it is untreated, take the form of diabetic retinopathy, diabetic nephropathy, diabetic neuropathy and cardiovascular complications such as heart attack, kidney disease and renal failure. With diabetes being a widespread threat to humanity, plant kingdom plays an important role in diabetes ailments. Modern allopathic medicine has no known cure for the diabetic patients. Therefore the present ethobotananical survey was carried out among the folk medicinal practitioners of Tangail district in Bangladesh for documentation of the indigenous medicinal plants used by them for the treatment of diabetes as well as to find out scientific validation of their usages over the years. It was found that the folk medicinal practitioners used a number of medicinal plants to lower the level of blood glucose of the patients without any knowledge on the mechanism of action of these plants. Key words: Ethnobotany; Diabetes; Insulin; Plant kingdom; Novel drugs. 56 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Investigation on Hypoglycaemic action of ethanolic extract of Catharanthus roseus on Normal and Streptozotocin Induced Type 2 Diabetic Rats. Ashraf Ul Kabir1*, Mehdi Bin Samad2, Ninadh Malrina D‟Costa1, Arif Ahmed2, Khorrul Jannat2, JMA Hannan1, 2 1 Department of Pharmacy Practice, North South University, Dhaka-1229, Bangladesh. 2 Department of Pharmaceutical Sciences, North South University, Dhaka-1229, Bangladesh. Abstract Diabetes is a widely prevalent endocrine disorder worldwide. Catharanthus roseus has been shown to possess antidiabetic activity. In our study, we aimed at discovering the underlying mechanism of its antidiabetic action. The study was carried out in accordance to recommendations of the international Association for the study of Pain. To evaluate the ability of Catharanthus roseus to lower acute glucose load and basal glucose we carried out the “Glucose tolerance test”, “Fasting Blood Glucose” respectively. The effect of sucrose absorption from various segments of the gut (the stomach, the upper 20 cm, middle, and lower 20 cm of the small intestine, the cecum, and the large intestine.) was determined by “Six Segment Test”. An “Intestinal Perfusion” technique was used to study the effect of Catharanthus roseus on intestinal absorption of glucose in 36 h fasted non-diabetic rats. Finally alteration of “GUT motility” was determined to see how long the food content would be in the intestines for sugar in food to be absorbed. Groups of data were compared using unpaired Student‟s t test and Mann– Whitney U test where appropriate. Where data were collected over a number of time-points, analysis was based on repeated measures ANOVA, with Bonferroni adjustment. . One-way ANOVA was performed and pair-wise comparisons to the control group made using Dunnett‟s test. Differences were considered significant at P<0·05. Administration of Catharanthus roseus (0·5 g/kg body weight) to normal and type 2 diabetic rats significantly (P<0.01) improved oral glucose tolerance. Basal blood level remained unaffected. Total remaining unabsorbed sucrose in the gastrointestinal tract of non-diabetic and type 2 diabetic rats, following oral sucrose loading (2·5 g/kg body weight) was significantly (P<0.05) increased by Catharanthus roseus extract. The extract significantly (P<0.05) suppressed the intestinal glucose absorption in both nondiabetic and type 2 diabetic rats. The extract also increased gastrointestinal motility (P<0.01).The present findings indicate that the Catharanthus roseus exerts hypoglycemic effects mediated through inhibition of carbohydrate absorption and by shortening the GUT transit time of food substance. It probably does not have any insulin secretagogue action since basal glucose level remained unchanged. Keywords: Intestinal Perfusion; Six Segment Test; Gut Motility; Andidiabetic; Catharanthus roseus. 57 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Evaluation of antipyretic activity and thrombolytic activity of Desmodium Pulchellum Benth Asma Ahmed*, Mohsina Mukti, FarihaTasnim, Tanzila Manzur, Satabdi Dey Lopa, Md. Mahbubur Rahman Department of Pharmacy, North South University, Baridhara, Dhaka-1229, Bangladesh. Abstract Desmodium Pulchellum Benth(Fabaceae), used in variety of ailments in folk medicinal practices by the tribes of Malays, Deori in different regions of Philippines,Java (Indonesia), India, was investigated for its antipyretic andin vitro thrombolytic potential.Ethanolic root extract of Desmodium Pulchellum Benth was administered at the dose of 500 mg\kg (p.o) by suspending the extract in distilled water. Brewer's yeast suspension (10 mg/kg) was used as a fever inducing agent in this experiment. The clot lysis activity was assessed by addition of the test material to the pre-clotted blood and incubation for 90minutes at 37°C and was expressed % lysis of clot. The ethanolic root extract and its partitionates at concentration 10mg/ml were tested.The ethanolic root extract of Desmodium Pulchellum Benth exhibited statistically highly significant (p < 0.001) antipyretic effect γ hours after administration in Brewer‟s yeast induced pyrexia in Long Evansrats. Addition of 100µl standard streptokinase(SK), a positive control (30,000 I.U.), to the clots and subsequent incubation for 90 minutes at 37°C, showed 66.84% lysis of clot.At the same time, distilled water was treated as negative control which exhibited negligible lysis of clot (3.87%). In this study, the methanol soluble fraction exhibited highest thrombolytic activity (84.13%) which showed greater activity than standard. The ethanolic extract has exhibited lysis of 41.95%.However, other partitionates also showed significant thrombolytic activity such as hexane soluble fraction (49.89%) and aqueous soluble fraction (49.89%).These results show that ethanol extract ofDesmodium Pulchellum Benthhas antipyretic and thrombolytic activity. Keywords: Desmodium Pulchellum Benth; Antipyretic activity; Thrombolytic activity; Brewer's yeast. 58 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Essential oil composition, antioxidant and cytotoxic properties of Alstonia scholaris (L.) R. Br. Atiqur Rahman1*, Shah Alam Siddiqui1, M. Oliur Rahman2, Mohammad Ahsanul Akbar3, Rafiquel Islam1, M. A. Sattar4 1 Department of Applied Chemistry and Chemical Technology, Islamic University, Kushtia 7003, Bangladesh. 2 Department of Botany, University of Dhaka, Dhaka 1000, Bangladesh. 3 Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Dhaka 1000, Bangladesh. 4 Jessore Science and Technology University, Jessore, Bangladesh. Abstract In this study essential oil composition, antioxidant and cytotoxic properties of the flowers of Alstonia scholaris (L.) R. Br. was investigated. The chemical composition of the oil was analyzed by GC-MS. The antioxidant potential was evaluated using two separate methods, inhibition of free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide radicals scavenging activities assay. Furthermore, the total phenolic compound was determined and expressed as gallic acid equivalent of dry weight of sample. The cytotoxicity of the oil was also determined through the brine shrimp toxicity assay. Twenty-six compounds representing 92.84% of the total oil were identified. In the first case of antioxidant potential assay, the IC 50 (inhibitory concentration) value of the oil was 1β.1 ± 1.β μg/mL. Among the extracts, the strongest activity was exhibited by the methanol extract (IC50:15.36 ± 1.3). In the superoxide radicals scavenging activities assay, the oil showed the strongest activity (IC50: β0.8γ ± 0.γl μg/mL) and methanol extract displayed stronger activity (IC50: γ0.47 ± 0.6 μg/mL) than other extracts. The LC50 (LC, lethal concentration) value of β.56 ± 0.1γ μg/mL indicated that the oil was highly toxic. It is suggested that the active compounds of the Alstonia scholaris flower exhibit potential antioxidant and cytotoxic properties. These substances can be considered as a potential antioxidant and cytotoxic agents as well as imminent candidate for cancer therapy. Keywords: Alstonia scholaris (L.) R. Br.; Antioxidant; Cytotoxicity; Lethal concentration. 59 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Pharmacological Screening of Methanolic Extract of Pulp of Phyllanthus acidus Farjana Afrin1, Antara Ghosh1, Md. Ariful Islam1, Mohammad Salim Hossain1,*, Md. Mizanur Rahman Moghol1 1 Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali3814, Bangladesh Abstract Phyllanthus acidus a plant belonging to the family Euphorbiaceae is traditionally used to cure many diseases. This study describes some the therapeutic effects of the pulp of Phyllanthus acidus. Objective of this study was to evaluate the possible use of this plant as hypoglycemic, anti-diarrheal, analgesic and anesthetic agent. The pulp of Phyllanthus acidus was extracted with methanol, which was used for the observation of Hypoglycemic, anti-diarrheal, analgesic and anesthetic activities. The methanolic crude extract of Phyllanthus acidus (pulp) were screened for hypoglycemic activity using swiss-albino mice. A reputed hypoglycemic agent Glibenclamide was used as a positive control. The crude extract of Phyllanthus acidus was tested for anti-diarrheal, analgesic and anesthetic activities. The extract exhibited anti-diarrheal activity was determined by the method of castor oil induced diarrhea in mice using Loperamide as a positive control, analgesic activity by tail immersion method using Morphine as a positive control, anesthetic activity by thiopental sodium–induced sleeping time test. Our results showed that methanolic extract of the pulp of this plant is active for lowering the glucose levels in experimental animals. The extract also showed moderate anti dirrheal, analgesic and anesthetic properties. Keywords: Phyllanthus acidus; Hypoglycemic; Anti-diarrheal; Analgesic; Anesthetic. 60 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Classical versus Modern Approach: Promises in Antibiotics (cephalosporin) Development and Commercialization A.S.M. Homaun Kabir Chowdhury*, C.M. Sabbir Ahmed, Md. Lemon Hasan Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh. Abstract Cephalosporins are a class of -lactam antibiotics originally derived from the fungus Acremonium, which was previously known as "Cephalosporium". It would be beneficial if the development process of cephalosporin is well understood by thorough analysis (catechol and isoxazolidin cephalosporin) and its subsequent commercialization process in the realistic sense. Clear concepts of classical and modern approach and importance of modern process of cephalosporin development (phytopharmacy, raw material supply, identity test, house control, chemical approach, preclinical-clinical development, patent policy) and commercialization (optimistic way) could pave the way for more accurate treatment, sustainable and rational uses of medicines. Keywords: Cephalosporin; -lactam; Phytopharmacy; Catechol; Isoxazolidin; Chemical approach; Classical approach; Modern approach. 61 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Integration of High Throughput Omics Sciences and In-silico design approach for Novel Target Identification, Validation and Drug Design R. M. Imtiaz Karim Rony* Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh. Abstract Identification and validation of key biomolecules related to diseases has always been the first step towards Drug Designing. Although tradition approaches have already proved to be successful for a limited spectrum of diseases, distinguishing the best target for complex disease such as cancer, neurodegenerative or cardiac diseases, muscular dystrophies, etc is still somewhat daunting. Often these fatal diseases are governed by a complex network of interconnected biomolecules (proteins, RNAs) and their highly regulated interaction and functional profile makes target identification and validation even challenging. Over the years Revolutionary advancement has been achieved in the field of omics studies such as Genomics, Proteomic and Metabolomics and some other emerging fields such as systems biology. Recent works has already confirmed that integration of genomics and proteomics study with basic clinical sciences can tremendously aid to identify novel target for specific disease by comparing healthy and diseased sample in genomic, proteomic or metabolic level. System level analysis of these biomolecular network not only helps to narrow down key molecules related to the disease along with their interacting partners, they also assist to screen out the most suitable target for drug design based on interaction hierarchy. Simultaneously, in silico drug design approaches which utilize modern bioinformatics tools and detailed structural information of a wide array of biomolecules can drastically enhance drug design process. Undoubtedly these high throughput studies have already raised a great deal of hope and such multidisciplinary approaches will definitely render us with the most effective drugs to fight against all prevailing life threatening diseases in near future. Keywords: Omics Sciences; In-silico design; Novel Target Identification; Validation; Drug Design. 62 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation An ethnomedicinal Survey of medicinal plant usage by folk medicinal practitioners in different Villages at Nalitabari Upazilla, Sherpur District, Bangladesh Shahin Mahmud1, Md Masud Rana1, Aysha Ferdoushi1* 1 Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Santosh, Tangail -1902, Bangladesh. Abstract Folk medicinal practitioners, well known as Kavirajes, form the primary line of health-care among a substantial section of the rural and urban population of Bangladesh. They rely on administration of medicinal plants for treatment of diseases. Since the medicinal plants used by the Kavirajes can differ widely even between adjoining areas, it was the objective of the present study to conduct an ethnomedicinal survey among the selected Kavirajes of different villages at Nalitabari Upazilla situated in Sherpur district ,Bangladesh. Informed consent was obtained and interviews were conducted of the Kavirajes with the help of a semi-structured questionnaire and the guided field-walk method. Plant specimens as pointed out by them were collected and identified at the Bangladesh National Herbarium. It was observed that the Kavirajes of the selected areas used a total of 115 plants distributed into 68 families for treatment of various ailments. The Liliaceae family provided the largest number of species (6); followed by the Cucarbitaceae , Fabaceae , Zingiberaceae family (5); Rosaceae , Myrtaceae , Apiaceae (4) and other of the families contributed single plant. Of the total 160 uses found in the present survey, leaves formed the most frequently used plant part (26.87%), followed by Fruits (18.12%) ,seeds (16.25%) , roots (13.75%) ,stems (11.27%), flower( 6.23%) ,Bark ( 5%) and whole plant (2.6%). Most of cases, a single plant part was used for treatment of any given ailment. Most of the plants were used to treat common ailments such as respiratory tract problems, gastrointestinal disorders, fever and loss of hair, vomiting, menstrual problems, skin disorders, dental diseases, cracked foot, bleeding, insect bites and bone fracture. However, a number of plants were also used to treat more complicated ailments like cardiovascular disorders, hepatic disorders, mental disease, epilepsy, piles, leprosy, diabetes and cancer or tumors. Folk medicine in Bangladesh has a history of usage going back thousands of years thus possess considerable potential for further scientific research for discovery novel compounds that may serve as the source of effective modern drugs. Key words: Folk medicine; Medicinal plants; Ailment; Sherpur; Bangladesh. 63 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Antibacterial activity of Bifidobacteria isolated from infant feces against pathogenic bacteria. K. M. Sakib1, M. Mizanur Rahman1*, Himel Nahreen Khaleque 2, Md. Monzur Morshed 2 1 Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia- 7003, Bangladesh. 2 Industrial Microbiology Section, Institute of Food Science and Technology (IFST), BCSIR, Dhaka. Abstract Bifidobacteria are important constituents of the intestinal microflora of humans and animals. These organisms are the predominant group of bacteria in the normal intestinal flora of healthy breast-fed newborns where they constitute more than 95% of the total population. The objective of this study was to isolate, identify and characterize probiotic organisms from infant feces and determine their activity against common pathogens. Twenty two (22) isolates of bacteria from feces of breast-fed infants were characterized by phenotypic, morphological and biochemical characteristics to identify them as Bifidobacteria. Antibacterial activity of these isolates against pathogenic organisms was tested by the agar well diffusion method as well as by the modified agar diffusion method. Among these, four isolates were randomly chosen for further study. Three of the isolates, B7, B9 and B11 were identified as Bifidobacteria adolescentis on basis of their carbohydrate fermentation patterns. B5 could not be identified due to its variable fermentation patterns. All isolates showed inhibitory activity against a variety of pathogenic test organisms, with zones of inhibition being between 12.02 to 25.03 mm. The highest and lowest zones of inhibition were both by Isolate B11 against Escherichia coli (25.03 mm) and Pseudomonas aeruginosa (12.02mm), respectively. The results of this study suggest that the isolated indigenous Bifidobacteria can be a source of natural antimicrobial agents with potential applications in Food or Pharmaceutical industries. Keywords: Probiotic organisms, Bifidobacteria, Natural antimicrobial agents 64 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Studies of the Antidiarrheal and Antimicrobial activity of Bauhinia acuminate Nazma Akter Tithi1, Mohammad Mamun Ur Rashid2, Md. Robiul Hossain2, Md. Abul Barakat Fahad2, Mohsina Mukti3 1 Department of Pharmacy, University of Asia Pacific, Satmasjid Road, Dhanmondi, Dhaka1205, Bangladesh. 2 Department of Pharmacy, International Islamic University Chittagong, 154/A College Road, Chittagong-4203, Bangladesh. 3 Department of Pharmacy, North South University, Bashundhara, Dhaka-1229, Bangladesh. Abstract This study evaluated the antidiarrheal and the antimicrobial study of the plant, Bauhinia Acuminata (Fabaceae). The methanolic extract of the plant was used for the study. The study involved the antidiarrheal (castor oil induced diarrhea, magnesium sulphate induced enteropooling) tests on the Swiss albino mice and the antimicrobial test which includes diffusion susceptibility test against seven strains of bacteria amongst which six of them is diarrheacausing. The study suggested that methanolic extracts was safe up to 2000mg/kg. The crude methanolic extract of Bauhinia acuminata showed a marked antidiarrheal activity at dose of 200 mg/kg and 400mg/kg- body weight as compared to the standard antidiarrheal agent Loperamide (dose:1mg/kg-body weight). The result showed significant (P < 0.01) reduction in animal model in magnesium sulphate induced enteropooling. In castor oil induced diarrhea, it showed reduction in dose dependent manner. Anti-diarrheal activity was present in the methanolic extract (200mg/kg and 400mg/kg) which indicate that the crude drug acted by causing decreased intestinal motility. Any kind of antimicrobial activity was not shown by the methanolic extract of leaf of this plant, Bauhinia acuminata. Keywords: Bauhinia acuminate; Antidiarrheal activity; Antimicrobial activity; Enteropooling. 65 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Prospects of medicinal plants (Momordica dioica & Couroupita guinensis) in the management of type 2 diabetes Md. Mynul Hassan1*, Begum Rokeya2 1 Biotechnology & Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh. 2 Professor & Chief Research Officer, Department of Pharmacology, BIRDEM, 122 Kazi Nazrul Islam Avenue, Dhaka-1000, Bangladesh Abstract Diabetes mellitus is one of the common metabolic disorders and 2.8% of the population suffers from this disease throughout the world. It is a chronic disease that requires long-term medical attention to limit the development of its devastating complications and to manage them when they do occur. It is a disproportionately expensive disease. Due to lack of organized health care systems in developing countries like Bangladesh, people with this choronic disease are among the worst sufferers in the society. Recent WHO and IDF reports show that the incidence of diabetes is increasing at an epidemic rate specially in developing countries probably due to rapidly changing life-style, food habit and environmental condition. So far, it is well known that oral hypoglycemic agents like sulphonylureas, biguanides and thiozolidindiones are still the major players in the management of Type 2 diabetes. However, most of them are costly and have lots of side effects. So there is a need for better oral antidiabetic agents. Plants have got a vast potential to provide source materials for antidiabetic agents. However, a limited number of these plant species have been studied and validated scientifically. World‟s Ethnobotanical information about medicinal plants reports that almost 800 plants are used in the control of diabetes mellitus. There are many other plants which are not still scientifically evaluated but they have “folk medicine reputation”. Momordica dioica and Couropita guinensis have got folkloric reputation as anidiabetic plants. Therefore, the aim of our study is to explore the antidiabetic effects of Momordica dioica fruit extracts and Couropita guinensis leaf extracts in streptozotocin induced diabetic rats and also try to find out some bioactive compounds of these plants. Keywords: Momordica dioica; Couroupita guinensis; Type 2 diabetes; Streptozotocin. 66 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Study on anti-diabetic activity of methanolic (CH3OH) extract of Blumea lacera (local name: Bonomula) leaves on mice model. Md. Nazmul Hasan1*, Mohammad Habibur Rahman1, Zakirul Islam1, Md. Faruk Miya1, Mita Munshi1, Afroza Rahman Nishi1 1 Department of Genetic Engineering & Biotechnology, Faculty of Biological Science and Technology, Jessore Science & Technology University (JSTU), Jessore-7408, Bangladesh. Abstract The present study was designed to investigate the hypoglycemic activity of methanolic extract of Blumea lacera (Bonomula) leaves on swiss albino mice model. The mice were administered with the extract of B. lacera at the dose of 200mg/kg & 400mg/kg body weight respectively in different group. We use 4 groups of mice (switch albino) to perform the experiment, i.e; (Control, standard, 200mg/kg & 400mg/kg body weight) group. We administered water to the control group, and Glyzid (80mg tablet) as 10mg/kg body weight to the standard group. After 1hr. we administered glucose (dextrose) solution as 2gm/kg body weight of mice. Afte 1hr. of glucose administration, blood was collected from the mice, and the serum was used to measure „blood glucose level‟. Keywords: Blumea lacera; Anti-diabetic activity; Methanolic (CH3OH) extract; Mice model. 67 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation A study on the antioxidant, antimicrobial and preliminary cytotoxic activity of Acanthus ilicifolius and Clerodendrum inerme, two selected mangrove plants from the Sundarbans Md Niamat Hossain1*, Kazi Didarul Islam1, Md Emdadul Islam1, Farah Sabrin2, Md Morsaline Billah1 1 Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh. 2 Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh. Abstract Acanthus ilicifolius (Family: Acanthaceae) and Clerodendrum inerme (Family: Verbenaceae) are two widely distributed plants of the Sundarbans mangrove forest. The present study was performed to evaluate some of the selected bioactivities of these two plants. The extracts of the plants were evaluated for potential antioxidant, antimicrobial and preliminary cytotoxic properties, using the DPPH free radical scavenging assay, disc diffusion assay and brine shrimp lethality bioassay respectively. Among the extracts, the methanol extracts of both species showed best free radical scavenging activity and the IC50 values were 26.30, 104.71 and 100 µg/ml, respectively, for A.ilicifolius leaf, stem and C.inerme. The chloroform extract of C.inerme showed maximum activity against gram positive Staphylococcus epidermis with zone of inhibition 26 mm for 1000 µg/disc. A.ilicifolius leaf chloroform extract showed activity against S. epidermis and Salmonella typhi. Chloroformic extract of A.ilicifolius stem showed activity against S. epidermis and Vibrio cholerae while methanolic extract showed activity against V.cholerae. Chloroformic extract of C. inerme showed activity against Escherichia coli, S. typhi and S. epidermis and methanol extract against S. epidermis. In the cytotoxic assay, chloroformic extract of C. inerme showed lowest LC50 value of 9.66 µg/ml. The petroleum ether extract of C. inerme and methanolic stem extract of A.ilicifolius showed potential preliminary cytotoxic activity as indicated by LC50 values at the concentration of 13.33 µg/ml and 11.5 µg/ml respectively. Keywords: Acanthus ilicifolius; Clerodendrum inerme; Antioxidant; DPPH; Antimicrobial; Cytotoxicity. 68 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Investigation on Hypoglycaemic action of ethanolic extract of Psidium guajava on Normal and Streptozotocin Induced Type 2 Diabetic Rats. Ninadh Malrina D‟Costa1*, Ashraf Ul Kabir1, Mehdi Bin Samad2, Farjana Akther2, Arif Ahmed2, JMA Hannan1, 2 1 Department of Pharmacy Practice, North South University, Dhaka-1229, Bangladesh. 2 Department of Pharmaceutical Sciences, North South University, Dhaka-1229, Bangladesh. Abstract Diabetes is a globally prevalent endocrine disorder. The Psidium guajava has been shown to possess antidiabetic activity. In our study, we aimed at discovering the underlying mechanism of its antidiabetic action. The study was carried out in accordance to recommendations of the international Association for the study of Pain. To evaluate the ability of Psidium guajava in lowering acute glucose load and basal glucose, we carried out the “Glucose tolerance test”, “Fasting Blood Glucose” respectively. The effect of sucrose absorption from various segments of the gut (the stomach, the upper 20 cm, middle, and lower 20 cm of the small intestine, the cecum, and the large intestine.) was determined by “Six Segment Test”. An “Intestinal Perfusion” technique was used to study the effect of Psidium guajava on intestinal absorption of glucose in 36 h fasted non-diabetic rats. Finally alteration of “GUT motility” was determined to see how long the food content would be in the intestines for sugar in food to be absorbed. Groups of data were compared using unpaired Student‟s t test and Mann–Whitney U test where appropriate. Where data were collected over a number of time-points, analysis was based on repeated measures ANOVA, with Bonferroni adjustment. . One-way ANOVA was performed and pair-wise comparisons to the control group made using Dunnett‟s test. Differences were considered significant at P<0·05. Administration of Psidium guajava (0·5 g/kg body weight) to normal and type 2 diabetic rats did not improve oral glucose tolerance significantly (P=0.08). Basal blood glucose remained unchanged. Total remaining unabsorbed sucrose in the gastrointestinal tract of non-diabetic and type 2 diabetic rats, following oral sucrose loading (2·5 g/kg body weight) was significantly (P<0.05) increased by Psidium guajava extract (0·5 g/kg body weight). However, the extract did not suppress the intestinal glucose absorption. The extract did not cause significant increase in the gastrointestinal motility. The present findings indicate that the Psidium guajava probably exerts its hypoglycemic effects through inhibition of sucrose digestion, not by inhibition of glucose absorption. Currently, we are conducting separate study on the effects of Psidium guajava on intestinal disaccharidase enzyme. Keywords: Intestinal Perfusion; Six Segment Test; Gut Motility,;Andidiabetic;Psidium guajava. 69 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Acute toxicity studies and calculations of LD50 values of fruit extract of Neoalsomitra clavigera Nitin Agrawal 1*, Subhash C. Mandal 1, H. Lalhlemawia 2, Vivekananda Mandal2 1 Pharmacognosy and Phytotherapy Research Laboratory , Division of Pharmacognosy, Department of Pharmaceutical Technology , Jadavpur University , Kolkata -700032 , India. 2 Department of Pharmacy, Regional Institute of Paramedical and Nursing Sciences, Zemabawk, Aizawl , Mizoram-796017, India. 3 Department of Pharmacy, Guru Ghasidas University, Bilaspur -495009, India. Abstract The present study is quite new and the plant has lots of unexplored things which can be use full for treatment of various ailments. This study has been taken because no such study has reported till date. The objective of the study was to evaluate acute toxicity produced and to calculate median lethal dose (LD50) of methanol extract of dried ripe fruits of Neoalsomitra clavigera (Wall) Hutch. (Cucurbitaceae) in Wistar albino rats. This study was carried out according to Organization for Economic Co-operation and Development (OECD). The powdered drug was exposed to soxhlet extraction with methanol under control conditions of temperature. The animals were divided in to five groups (5 animals each group), the one group, out of five was treated as control group and the remaining four were treated as extract treated group and treated with 5, 50, 300 and 2000 mg/k.g body weight respectively. From first day of dosing, the animals were observed for physical changes, routine habit toxic effects and mortality up to 15 days. On 15th, the animals were sacrificed under excessive ether anesthesia and total blood was collected by puncturing posterior vena cava for serum biochemistry studies and organs like heart, liver, stomach, kidneys, spleen, and adrenal glands were dissected and exposed to histological studies. After statistical analysis, all the significant values were compared with control group. Simultaneously the LD50 value was calculated. The results of serum biochemical studies showed significant changes after going above to certain dose level and histopathological studies also showed significant differences. On the basis of these results the lethal dose was also calculated. On the basis of above study it is concluded that the drug is very lethal at very low doses. Keywords: Neoalsomitra clavigera; LD50 value; acute toxicity. 70 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation An Ethnomedicinal Survey of Medicinal Plants used by Folk Medicinal Practitioners of different villages in Tangail district, Bangladesh to treat Jaundice and Allergy Mohammad Nazmul Hasan1*, Al-amin1, Md. Arif Khan1, Aysha Ferdoushi1, Md. Morsaline Billah2, Farah Sabrin1 1 Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University. Santosh, Tangail -1902, Bangladesh. 2 Biotechnology Bangladesh. and Genetic Engineering Discipline, Khulna University, Khulna-9208, Abstract Jaundice and allergy are most common diseases among Bangladeshi population. The disease is widely also prevalent in both urban and rural regions in the country. In rural areas, these diseases sometimes lead to life-threatening critical situations. Different allopathic drugs were used to treat them, but play a little role in the managemnet of chronic illness. So the predominantly rural population of the Bangladesh usually relies on folk medicinal practitioners (Kavirajes) for treatment of this affliction. In this study, semi-structured questionnaire was employed to conduct surveys among folk medicinal practitioners of the different villages in Tangail district, Bangladesh. Informed consent was obtained from the practitioners prior to interviews. It was observed that the Kavirajes of the selected areas used a total of 16 plants distributed into 10 families for treatment of jaundice ailments. The Rutaceae family provided the largest number of species (3) and most of the families were contributed single plant. On the other hand of 10 plants weere distributed into 9 families for treatment of allergy ailments. The various plant parts were used to treat jaundice and allergy included whole plant, leaves, barks, roots, flowers and, fruits. The survey highlights the considerable differences in medicinal plant usage by Kavirajes of different villeges of tangail district. As such, these medicinal plants used by the Kavirajes need to be scientifically studied in a more rigorous manner, and efforts should be made to collect information from other Kavirajes in other district of Bangladesh for medication of such disease, which comprises of a number of ailments prevalent worldwide and suggests that considerable potential exists for modern scientific studies to be carried out on the medicinal plant species in the quest for better drugs. Key words: Jaundice; allergy; folk medicine; medicinal plants, Bangladesh. 71 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation In vitro evaluation of Thrombolytic and Membrane Stabilizing activities of fruits of Averrohoa bilimbi Pankaj Chandra Debnath1, Sayed Koushik Ahmed1, Mohammad Salim Hossain1,* Md. Ariful Islam1, Md. Mizanur Rahaman Moghol1 1 Department of Pharmacy, Noakhali Science & Technology University, Sonapur, Noakhali3814, Bangladesh Abstract Averrohoa bilimbi has extensive use in traditional medicines for different purposes. This study is used to evaluate some of these usages. Primary objective of this study is to investigate possible thrombolytic and Membrane Stabilizing activities of Averrohoa bilimbi. In vitro method was used for this study using human red blood cells collected from healthy volunteers. Dried powder of the plant was extracted with methanol and the concentrated methanolic extract was fractionated by the modified Kupchan partitioning method to provide pet-ether, ethyl acetate, chloroform and aqueous soluble fractions which were used for both tests. Streptokinase was used as standard in thrombolytic test whereas acetyl salicylic acid was for membrane stabilizing test. All fractions revealed significant thrombolytic activity when compared to control (distilled water) and standard (streptokinase). However, ethyl acetate fraction showed maximum thrombolytic activity with a value of 28.65% (P<0.01) lysis of blood cells while standard streptokinase exhibited 49.08% (P<0.001) lysis of clot. All fractions inhibited heat as well as hypotonic solution induced hemolysis of RBC in vitro. The methanolic extract inhibited 20.76% (P<0.001) and 37.71% (P<0.001) hemolysis of RBC caused by heat and hypotonic solution, while compared to 34.49% (P<0.001) and 77.52% (P<0.001) inhibition of hemolysis caused by standard acetyl salicylic acid, respectively. This plant may be a potential source of thrombolytic and membrane stabilizing principles. Keywords: Thrombolytic; Membrane Stability; Averrohoa bilimbi; Streptokinase. 72 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Extraction and Isolation of lead compounds from Solanum nigrum Linn. Partha Pratim Maiti1, Subhash C Mandal1, Anup K Das1, Nitin Agrawal1, Vivekananda Mandal2 1 Phrmacognosy and Phytotherapy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700 032, India. 2 Department of Pharmacy, Guru Ghasidasi University, Bilaspur, Chattisgarh, India. Abstract Plants are the living factories for the production of numerous secondary plants metabolites. These secondary plants metabolites are used in the treatment of various ailments. Solasodine, solanine, solasonin, solanidine etc are the leading bioactive compounds of solanum nigrum linnhave been extracted and isolated by various economical chromatographic techniques. The present study extraction and isolation of lead compounds from Solanum nigrum Linn has been taken because this study is not scientifically reported previously. The aim of the present study designed in the following like extraction of crude drug of Solanum nigrum by using various solvents. Phytochemical screening of various extracts of Solanum nigrum. Identification, isolation and purification of cardioprotective lead molecule development form the Solanum nigrum and its extracts. The shade-dried berries of Solanum nigrum were powdered and extracted by using solvents of increasing polarity. The extracts obtained was named as petrolium ether extract, benzene extract, chloroform extract, acetone extract, ethyl acetate extract, methanol extract, ethanol extract and water extract. The phytochemical investigation was done for confirmation of major phytochemicals groups like glycosides, alkaloids, carbohydrates, proteins etc. After screening the various extract obtained were exposed to fractionation and isolation by normal phase column chromatography from low polarity to high polarity of solvents. The collected column fractions were exposed to thin layer chromatography to identify the lead compounds and the isolated the following compounds SNA, SNB, SNC. The results of phytochemical analysis of different extract of Solanum nigrum was positive for alkaloids, carbohydrates, glycosides, tannis etc, The compounds isolated from different extract of Solanum nigrum belongs to alkaloids, steroidal alkaloid, glycoalkaloid, glycoside groups. The compounds isolated in this study are still in need to evaluate by pharmacological screening methods. Keywords: Solanum nigrum; Lead compound; cardioprotective; Phytochemical. 73 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Diabetes: Advances and Emerging opportunity in Diabetes Research C.M. Sabbir Ahmed ⃰ , Md. Lemon Hasan, A.S.M. Homaun Kabir Chowdhury Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh. Abstract Diabetes is now an established health concern worldwide and emerging as an epidemic world over. The management of diabetes is still a major challenge. This study has been conducted with the aim to figure out with general information on recent diabetic research network based on relevant research articles. Therapeutic herbal plants for diabetes treatment is most common and south Asia is the prominent user due to availability of herbal plants in this region. Anti diabetic compound extraction technique, acting mechanism study for drug development and commercialization can be area of research. Stem cells research has been reassuring in the field of diabetes treatment. Genetic factor are also responsible for diabetes. Polymorphism study of different genes may lead to know about contribution of gene in diabetes oriented disease. Recently identified hormone betatrophin opens a new door of research in diabetes treatment. Betatrophin induces pancreatic beta cell division and the rate of division increased 30 times in insulin-resistant mice. Drug development from this hormone can be excellent care for diabetes. Keywords: Diabetes; herbal plants; anti diabetic compounds; development; stem cells; polymorphism; betatrophin. 74 extraction technique; drug International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Antioxidant, antimicrobial and preliminary cytotoxic potential of Caesalpinia crista and Cynometra ramiflora from the Sundarbans Sadia Afrin1*, Farah Sabrin2, , Kazi Didarul Islam1, Md Emdadul Islam1, Md Morsaline Billah1 1 Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh. 2 Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Santosh, Tangail -1902, Bangladesh. Abstract In this study, two Mangrove climber Caesalpinia crista and Cynometra ramiflora from the same family (leguminosae) were selected based on their traditional uses. In order to find out the scientific background of such uses, the present study was performed to study some of the bioactivities (antioxidant, preliminary cytotoxicity and antimicrobial activity) of these plants. Medium polar solvent (chloroform) extract and polar solvent (methanol) extract of two plants were taken for the study. In vitro antioxidant potential was evaluated by DPPH free radical scavenging and ferric chloride reducing power assay. Preliminary cytotoxicity and antimicrobial activity were conducted by using brine shrimp lethality and disc diffusion bioassay respectively. Methanolic extract of C.ramiflora (stem) obtained higher free radical scavenging activity (IC50:21.677µg/ml) and also had a capability to reduce Fe3+ to Fe2+ with reduction activity ranged (82.697% – 245.454%). Methanolic extract of C.crista showed moderate results in both antioxidant assays. In preliminary cytotoxic assay, methanolic extract of C.ramiflora and C.crista were found to show potential activity and LC50 values of both plants were same (~4µg/ml). Chloroformic extracts of C. ramiflora(leaf and stem) showed best antimicrobial activity against Bacillus megaterium and Salmonella typhi. Chloroformic and methanolic extracts of C.crista showed significant activity against Streptococcus aureus and Staphylococcus epidermis respectively. This research signifies that the plant extracts which have significant antioxidant property may show cytotoxicity. Keywords: Caesalpinia crista; Cynometra ramiflora; antioxidant; cytotoxicity; antimicrobial. 75 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Isolation of Phytomarker Vasicine from Adhatoda vasica L and standardization of its market preparations. B. K. Sajeeb1, Subrata Vadra1, Uttam Kumar1 , Sajal K. Saha2 , Shimul Halder1 , A. S. S. Rouf1 , M. Aftab Uddin3 , Sitesh C. Bachar1* 1 Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka1000, Bangladesh. 2 Dept. of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh. 3 Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh. Abstract Traditional medicines derived from medicinal plants are used by about 60% of the world‟s population. Various types of macroscopic, microscopic, physical, chemical and microbiological analyses are recommended for quality control of herbal medicines by several regulatory authorities. Adhatoda vasica L. contains vasicine which acts as a. The present study reveals an improved method for the identification and quantification of vasicine from Adhatoda vasica L. Alkaloid vasicine and vasicinone were identified and isolated from the methanolic extract of the herb followed by column chromatography with R f values of 0.31 and 0.78 respectively. In HPLC the phytomarker vasicine exhibited 7.4 min retention time (RT) with mobile phase methanol : water in a ratio of 40: 60 with flow rate of 0.5ml/min. The compound vasicine was ascertained with spectral analysis FTIR and LC-MS/MS. Similarly the phytomarker vasicinone was also identified and ascertained with spectral analysis. The retention time of vasicinone was 10.6 min with same mobile system and flow rate. Five market preparations containing Adhatoda vasica L. were collected and subjected for analysis. Specific amount of the liquid preparation was taken from each and ten times diluted with distilled water. The solution was acidified by 1% citric acid solution. The acidified solution was then basified with ammonia (25% solution) followed by extraction with chloroform. The organic layer was separated and evaporated to dryness. Dried organic fraction was then ten times diluted with methanol and analyzed by HPLC with a solvent system containing methanol: water in a ratio of 40: 60 with flow rate of 0.5ml/min. The RT of major peak was found at 7.4 min, which confirmed the presence of vasicine in market preparations. The identification and quantitation of phytomarker vasicine in the market preparations was confirmed by comparing its retention time and peak area with standard vasicine (Germany). The amount of vasicine in five different market preparations of sample-1, sample-2, sample-3, sample-4 and sample -5 were determined by using a standard calibration curve of vasicine from concentration range 75 to γ00 μg/ml (R2=0.982). The five commercial products contained vasicine 3.83g, 1.76g, 2.17g, 3.13g and 1.08g in 100mL preparation respectively. Keywords: phytomarker vasicine; Adhatoda vasica L; FTIR; microbiological analyses. 76 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation HPLC profiling analysis and identification of polyphenols in Ranunculus sceleratus Linn. Sanjib Saha1*, Khirul islam1, Md. Anisuzzman1, Md. Morsaline Billah2 1 Pharmacy Discipline, Khulna University, Khulna-9208, Bangladesh. 2 Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh. Abstract Ranunculus sceleratus Linn. belonging to the family of Ranunculaceae is an annual plant, native to North America, Eurasia, and Asia, where it grows especially in wet and moist habitat. The aim of the present study was to identify polyphenols through high performance lipid chromatography (HPLC) profiling analysis. Ethanol extract of the aerial parts of R. sceleratus was prepared through hot extraction technique using Soxhlet extractor. HPLC technique was utilized to get chromatographic profile of the polyphenols present in the extract. HPLC profile of the extract was compared with the profile of the standard polyphenols. Polyphenols were identified by comparing the retention time of the compounds with the standards. Gallic acid, catechin, caffeic acid, vanillic acid, epicatechin, para-coumaric acid, rutin hydrate, ellagic acid, and quercetin hydrate were used as standard polyphenols. Caffeic acid, rutin hydrate, and quercetin hydrate were identified in the ethanol extract as compared with the HPLC profile of the standards. Retention time of these three polyphenols was 16, 21, and 25 min, respectively. HPLC profile analysis clearly demonstrated that the aerial part of R. sceleratus contains some polyphenols. Keywords: Ranunculus sceleratus, Ranunculaceae, HPLC, Polyphenols. 77 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Antifungal activity and chromatographic analysis of I. Sepiaria koenig ex. Roxb. In concern to the folklore claim Sayani Majumder1*, Ashok B K1, Nishteswar K1 1 Department of Dravyaguna, I.P.G.T. & R.A., Gujarat Ayurved University, India. Abstract In one of the Ayurvedic text it is mentioned that the root powder of the plant is administered in case of leucorrhoea and Candida albicans, yeast like fungal stain is generally responsible for this pathological leucorrhoea. By taking consideration of these points, the present study was carried out. This study was undertaken to know antifungal effect of methanolic extract of root of I.sepiaria against the fungal strain Candida albicans and HPTLC analysis also carried out. The whole plant of I. sepiaria was collected from the campus of Gujarat Ayurved University. The collected samples were authenticated by the Botanical Survey of India, Office of the Scientist„F‟, Central National Herbarium, Botanic Garden, Howrah, West Bengal, specimen No.CNH/104/2011/Tech.II/581. The roots were collected and extracted with methanol to assessed antifungal activity by agar well diffusion method. The different solvent extracts were subjected for chromatographical separation and confirmation of chemical compounds by using various solvents and spraying reagents under visible and UV light of long and short wavelengths by following standard procedures. The result shows that the root of I. sepiaria is having phytoconstituents like alkaloids, carbohydrates, glycosides, phenolic compounds etc and HPTLC study of the different extract showed the presence of spots at different Rf values. The methanolic extract of root was found to possess significant antifungal activity. The methanolic extract of root of I. sepiaria is having very good antifungal activity and this supports the use of root in the treatment of leucorrhoea. Further researches are needed to explore the phyto constituents particularly responsible for the observed activity profile. Keywords: Ipomea sepiaria, leucorrhoea, Candida albicans, HPTLC. 78 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Antioxidant potential of common fruity vegetables in Bangladesh Sheikh Julfikar Hossain 1,*, Mst. Shahina Sultana1, M. Abu Taleb1, M. Habibul Basar1, M. Golam Sarower2 and Sk. Amir Hossain1 1 Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh. 2 Fisheries & Marine Resource Technology Discipline, Khulna University, Khulna-9208, Bangladesh. Abstract The amount of phenolics, flavonoids, anthocyanins in ethanol extracts and antioxidant activity of both ethanol and lipophilic extracts of common fruity vegetables in Bangladesh were studied. Among the ethanol extracts of 15 fruity vegetables, M. oleifera had highest total polyphenol content (85.05 mg gallic acid equivalent (GAE)/g extract) followed by L. acutangula (61.74 mg GAE/g extract) and A. esculentus (48.92 mg GAE/g extract). L. acutangula had high content of flavonoids (14.46 mg (+)-catechin equivalent (CE)/g extract), which was almost similar to L. siceraria (13.67 mg CE/g extract) followed by A. esculentus (11.95 mg CE/g extract) and S. melongena (11.42 mg CE/g extract). Highest anthocyanins content was in F. hispida (2.22 μmol/g extract) followed by S. melongena (1.04 μmol/g extract). Ethanol extracts of A. esculentus, F. hispida, L. acutangula, L. siceraria and S. melongena exhibited high DPPH free radical scavenging activity with IC50 of 70.4, 64.9, 70.4, 64.9 and 94.γ μg/mL respectively, whereas for the same lipophilic extracts of F. hispida and S. melongena showed lowest (37 μg/mL) IC50 followed by M. oleifera (47.6 μg/mL), L. siceraria (57.5 μg/mL) and A. esculentus (6γ.γ μg/mL). These vegetables also showed high reducing powers, NO scavenging and total antioxidant capacity. Therefore, the top five potential fruity vegetables consist of both hydrophilic and lipophilic antioxidant(s), the order being F. hispida > M. oleifera > A. esculentus, L. acutangula > L. siceraria > and S. melongena. Keywords: fruity vegetables; Antioxidant potential; S. melongena; L. siceraria; L. siceraria. 79 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation A study based on the ethnomedicinal use of herbs of the Sundarbans mangrove forest Shormi Fouzder*, Md Niamat Hossain, Sadia Afrin, Kazi Didarul Islam, Md Emdadul Islam, Md Morsaline Billah Biotechnology & Genetic Engineering Discipline, Khulna University, Khulna-9208,Bangladesh. Abstract From ancient time man has been using plants as a potential mean of ailment for their various sorts of disease. The healing properties of certain plants were identified, noted and conveyed to the successive generations. We can see the ample evidences in many written documents preserved monument that contain ethnomedicinal use of different medicinal plants e.g. Ayurveda. Still man is searching for new source of natural products in plants for designing more effective drugs. The following study is based on the ethnomedicinal use of herbs of the Sundarbans mangrove forest. The entire study includes twenty four herbs of the Sundarbans mangrove forest while 6 plants have been reported to be vital depending on the number of compounds isolated and traditional medicinal uses. The plants are Caesalpinia crista, Derris trifoliate, Portulaca oleracea, Cassia tora, Acalphya indica , Euphorbia hirta. The objective of the study is to investigate the potential of these plants to be used as novel sources of drug and to inform the latest progress to the researchers for further work on these plants as a promising alternative. Keywords: Ethnomedicine; ayurveda; natural products; herbs; mangrove forest; traditional medicine;drug design. 80 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Study on antibacterial activity of common medicinal plants used to treat diabetes in bangladesh KM Taufiqul Arif1, Sheikh Julfikar Hossain1* 1 Nutraceuticals and Bioresource Technology Laboratory, Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh. Abstract Medicinal plants, besides having therapeutic values against diabetes mellitus may have other pharmacological actions. To compare antibacterial activity of antidiabetic plants in Bangladesh, methanol extracts of them were tested against six pathogenic bacteria. Among the extracts, Coccinia indica (leaves), Gymnema sylvestre (Bark), Swertia chirata (whole part) and Syzygium cumini (seed) showed strong inhibition against Salmonella typhi, S. paratyphi A, Staphylococcus aureus and Shigella dysenteriae in disc diffusion test at the concentration of 400 μg/disc. No extract inhibited the growth of Vibrio cholerae. The lowest IC50 value (5.47 mg/mL) was found in C. indica against S. paratyphi A. Since, C. indica, G. sylvestre, S. chirata and S. cumini were found potential, they had been fractionated into five fractions namely hexane, diethylether, chloroform, ethylacetate and methanol. Among the fractions, methanol fraction of S. cumini potentially inhibited the growth of all four bacteria at the concentration of 400 μg/disc, whereas, S. paratyphi A was inhibited by C. indica and S. typhi by both G. sylvestre and S. chirata. However, the methanol fractions showed lesser inhibition than that of methanol extracts against the bacteria which entails the idea of synergistic action. Since, methanol fractions of the four plants were the most potent, their IC50, MIC and MBC values were determined. Altogether, among the common antidiabetic plants, S. cumini had potent antidiabetic activity that might be used in treating both diabetes and bacterial infections. Keywords: Diabetes; Swertia chirata; Coccinia indica; pharmacological actions; Shigella dysenteriae 81 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Protective effect of standardized Wattakaka volubilis leaf petroleum ether cold macerated extract against experimentally induced diabetes in the rat. Velmani Gopal1*, Vivekananda Mandal2 and Subhash C. Mandal1 1 Pharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700032, India. 2 Institute of Pharmacy, Guru Ghasidas Central University, Bilaspur 495009, India. Abstract Wattakaka volubilis is widely used in Indian traditional medicine to treat pain, cough, fever, dyspepsia and diabetes. The aim of the study was to evaluate antidiabetic properties of extract of W. volubilis. The present study investigated the protective effect of W. volubilis leaf extract against Streptozotocin (STZ) induced diabetes in rats. The petroleum ether cold macerated extract (PEME) of W. volubilis was evaluated to quantify lupeol by HPTLC method. Male Wistar rats were divided into five groups (with six rats each) and fed at libitum: the control (0.9% saline), STZ treated rats with or without supplementary PEME of W. volubilis and metformin (50, 100 and 250 mg/kg body weight) for three weeks. The blood-glucose, α-amylase, alanine transaminase (ALT), alkaline phosphatase (ALP) and bilirubin levels were measured on 7, 14 and 21-day of PEME treatment on STZ treated rats. Antioxidant properties were assessed by estimating hepatic catalase and lipid peroxidation. Histopathological changes in the liver were examined with hematoxylin-eosin staining. Morphological changes in the liver were also examined with glutaraldehyde fixation. The PEME had 382.82 μg of lupeol, treatment of PEME in experimental rats by oral injections for 21 days showed reductions in the levels of serum biochemical markers. PEME treatment also significantly recovered catalase and decreased lipid peroxidation towards normal. Histopathology and scanning electron microscopy results showed that PEME administration suppressed the abnormal liver cellular degenerations in STZ treated rats. These results suggest that PEME has a protective effect over STZ-induced diabetes. Keywords: Wattakaka volubilis, PEME, Lupeol, STZ, Liver, Scanning Electron Microscopy. 82 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Investigation on Hypoglycaemic action of ethanolic extract of Beta vulgaris on Normal and Streptozotocin Induced Type 2 Diabetic Rats. M.M. Zahid Hassan1*, Ninadh Malrina D‟Costa1, Mehdi Bin Samad2, Ashraf Ul Kabir1 , Khorrul Jannat2, Mohammad Rajib jahan2, JMA Hannan1, 2 1 Department of Pharmacy Practice, North South University, Dhaka-1229, Bangladesh. 2 Department of Pharmaceutical Sciences, North South University, Dhaka-1229, Bangladesh. Abstract Diabetes is a widely prevalent endocrine disorder worldwide. The Beta vulgaris has been shown to possess antidiabetic activity. Here we aimed at elucidating the underlying mechanism of its antidiabetic action. The study was carried out in accordance to recommendations of the international Association for the study of Pain. To evaluate the ability of Beta vulgaris to lower acute glucose load and basal glucose, we carried out the “Glucose tolerance test”, “Fasting Blood Glucose” respectively. The effect of sucrose absorption from various segments of the gut (the stomach, the upper 20 cm, middle, and lower 20 cm of the small intestine, the cecum, and the large intestine.) was determined by “Six Segment Test”. An “Intestinal Perfusion” technique was used to study the effect of Beta vulgaris on intestinal absorption of glucose in 36 h fasted nondiabetic rats. Finally, alteration of “GUT motility” was determined to see the time food content would be in the intestines for sugar in food to be absorbed. Groups of data were compared using unpaired Student‟s t test and Mann–Whitney U test where appropriate. Where data were collected over a number of time-points, analysis was based on repeated measures ANOVA, with Bonferroni adjustment. . One-way ANOVA was performed and pair-wise comparisons to the control group made using Dunnett‟s test. Differences were considered significant at P<0·05. Administration of Beta vulgaris (0·5 g/kg body weight) to normal and type 2 diabetic rats significantly (P<0.01) improved oral glucose tolerance. It also significantly lowered the basal glucose in the test subjects. It did not significantly change the total remaining unabsorbed sucrose in the gastrointestinal tract of non-diabetic and type 2 diabetic rats, following oral sucrose loading (2·5 g/kg body weight). Beta vulgaris did not significantly suppress the intestinal glucose absorption in both non-diabetic and type 2 diabetic rats. However, the extract significantly (P<0.01) increased the gastrointestinal motility of both type 2 diabetic and nondiabetic rats. The present findings indicate that the Beta vulgaris does not exert its hypoglycemic effects by inhibition of absorption, rather, probably, through enhancing insulin secretion or sensitivity. Keywords: Intestinal Perfusion, Six Segment Test, Gut Motility, Antidiabetic, Beta Vulgaris. 83 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Ethno-medicinal plants of indigenous population of the Tripuri Mohsina Mukti1*, Bijoy Chakraborty2, Md. Reyad-ul- Ferdous1 1 Department of Pharmacy, North South University, Baridhara, Dhaka, Bangladesh. 2 Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka-1205, Bangladesh Abstract This study was carried out in order to determine various medicinal plants and formulations used amongst the indigenous people specifically known as Tripuri residing in the wholeRajbari district of Bangladesh. Tripuris people descended from their ancestor inhabitants of theTwipra Kingdom in North-East India and ultimately dispersed in Bangladesh. They are also said to belong to Bodo-Kachari ethnicity in the North-East India. It is also believed that Tripuris descended from Bodo group of people, who are considered to be the ancestors of the people of Assam (India), Myanmar and Thailand. The survey work was carried out by interviewing healers with the help of a structured questionnaire and the guided field-walk method. The number of Tripuri community healers interviewed was four amongst a small colony population of 227 people in the area.The community of Tripuri forms the third largest tribal group inhabiting the Chittagong Hill Tracts region of Bangladesh. Plants of various families were revealed to be used amongst which Amaranthaceae,Asparagaceae, Asteraceae,Rutaceae,Solanaceae are mentionable. Some of the medicinal plants used in the formulations and the ailments treated includesGlycosmispentaphylla (constipation),Smilax zeylanica (pain, arising from evil wind), Clerodendrumviscosum (helminthiasis, jaundice), Asparagus racemosa (hypertension),Daturametel (oedema, inflammation), Crateva Magna ( paralysis, severe pain, rheumatic pain),AchyranthesAspera (dysentery) (toothache), Sennatora (leucorrhea, physical weakness,frequent urination), Drynariaquercifolia ( blood dysentery),Vernoniapetula (crying of children when afraid). Their medicinal practices not only comprised of medicinal plants and other ingredients, but were also accompanied by activities such as incarnation. Thorough observation of medicinal plant uses of tribal community healers can reveal various bioactive compounds, as well as better utilization of natural resources. Keywords: Tripuri; Crateva Magna; Spilanthesacmella; Asparagus racemosa; Clerodendrum viscosum. 84 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation A Study on Hyperglycemic Activity of Methanolic and Ethanolic Extract of Lasia spinosa Leaves in Mice Mita Munshi1, Mohammad Habibul Islam1, Zakirul Islam1, Md.Faruque Miya1, Afroza Rahman Nishi1 , Sabrina Shah 1, Md.Nazmul Hasan*1,2 1 Deptarment of Genetic Engineering and Biotechnology, Faculty of Biological Science and Technology, Jessore Science and Technology University ,Jessore-7408, Bangladesh. 2 Faculty of Agriculture, George-August-Universitat Goettingen, Goettingen, Germany. Abstract Lasia spinosa , locally known as Kohila is a stout, marshy plant with a creeping spiny rhizome. The tender leaves and rhizomes are used as a vegetable and are recommended for a variety of diseases in ayurvedic medicine. The present study was carried out to determine the hyperglycemic effects of methanolic and ethanolic extract of leaves of L. spinosa plant in oral glucose tolerance tests in glibenclamide (standard) -induced on white swiss albino mice. The experiment was designed as a dose dependent manner (200 mg/kg and 400 mg/kg body weight ) for each extracts and the route of administration was oral gaveging . The serum blood glucose level was measured by glucometer after 120 minutes of gaveging of extract and standard . Maximum hyperglycemic activity was observed at both of the doses compare to the control. The extracts demonstrated a significant (p<0.001) hyperglycemic activity in mice and the methanolic extract showed the more activity than ethanol.The above mentioned findings indicate the at the leaves extract of Lasia spinosa possesses hyperglycemic effect that support its use in traditional treatment processes. Keywords: Hyperglycemic effect, Lasia spinosa, methanolic extract, ethanolic extract . 85 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Medicinal plants used amongst the Bagdi community healers in Rajbari district of Bangladesh Asma Ahmed1, Topi Rizan Alam1, Md. Naimul Islam2, Mohsina Mukti1* 1 Department of Pharmacy, North South University, Baridhara, Dhaka, Bangladesh. 2 Department of Pharmacy, International Islamic University Chittagong, 154/A College Road, Chittagong-4203, Bangladesh. Abstract This study was carried out in order to determine various medicinal plants and formulations used amongst the indigenous people specifically known as Bagdi residing in the RajbariSadar in Rajbari district of Bangladesh. Bagdis descended from people who natively speak languages from Dravidian language family and arefound scattered in different regions of the whole country.The survey work was carried out by interviewing healers with the help of a structured questionnaire and the guided field-walk method. A total of 53 medicinal plants and 20 formulations were mentioned to be used by the healers for treatment of various ailments. The identified plants belonged to 28 families which comprise aquatic and non- aquatic plants. TheLeguminosae and Nymphaeaceaefamilies contributed a maximum number of 5 plants and are followed by Acanthaceae family with 4 plants.Some of these non-aquatic plants included Aristolachia indica (snake bite),Oxalis Coniculata(poisonous animal bites including snakes, dogs, cats),Banlerialupulina,Elensineindica (inflammation), Calotropisgigantean (inflammation), Alocasiamacrrorrhiza (rheumatoid arthritis), Kalanchoepinnata (abdominal pain), Alestoniascholaris ( Jaundice), Mimosa diplotricha (Uterine problems,closure of uterine passage), Persicariaglabra (severe pain including rheumatic pain), Ruelliatuberosa( skin diseases), Madhucaindica ( cardio- tonic), Bacopamonniera ( any sort of CNS disorder), Alternantheraphiloxeroita ( hepatitis), Eclipta prostate (rheumatic pain, burning sensation during urination), Vitexnegundo ( diabetes, black fever), Sennaalata (scabies), AndrographisPaniculata (common cold, fever, cough), PandanusFoetidus (cough). The aquatic plants included Ipomeaaquatica (piles), Nymphaceastellata (piles), Achyranthesaspera(tonsillitis), Nypmphaceae Nohucali( constipation), Nymphaceae Pubescens (leucorrhea, physical weakness, micturition), Nelumbo nucifera ( Cardiac diseases, cardio-tonic,weakness), Euryale ferox ( impotency for women). Many of the above mentioned plants used in the various traditional medicinal practices as well as contributed to development of modern allopathic medicines today. Fruitful isolation of novel compounds urged by necessity may lead to the discovery of more efficacious drugs. Keywords: Medicinal plants ;Aristolachia indica; NymphaceaePubescens; Euryale ferox. 86 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Quantitative Determination of Gallic Acid in Herbal Plant Materials and Evaluation of Market Preparation Triphala and Amlaki Rasayan Containing it Jyotirmoy Sarker, Subrata K Bhadra, A S S Rouf , Sitesh C Bachar* Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh. Abstract The quality control parameter of herbal medicine has become an important safety concern worldwide. The regulatory authorities of the developed countries emphasize the establishment of the quality parameters as prerequisite for patient safety. Traditionally herbal medicines are practiced in our country. But there are no specific quality parameter established or practiced in our country. As an inception a reversed phase high performance liquid chromatography method has been developed for the estimation of gallic acid in herbal preparations containing the fruits of Phyllanthus emblica, Terminalia chebula and Terminalia bellirica. For doing this at first the amount of gallic acid in the three fruits has been determined and later the amount of gallic acid found in the market preparations (Triphala and Amlaki Rasayan) are compared with these. A C18 column (250mm × 4.6 mm i.d., 5 µ particle size) was used with mobile phase composed of acetronitrile : acetic acid (0.1 %) in a ratio of 20 : 80 at a flow rate of 1 mlmin -1. The UV detection was performed at 272 nm and the retention time of gallic acid was found 3.8 min (approx). According to the result the highest amount of gallic acid was observed in Terminalia bellirica ( 2.22 to 2.34%) and the lowest amount was found in Terminalia chebula (1.44 to 1.54 %). Most of the market preparations showed significant variation in gallic acid content. This analytical approach can be used as a quality control tool in all kinds of herbal medicine manufacturing industry for ensuring the quality of plant raw materials and the finished product as a routine practice. Keywords: Gallic Acid; Phyllanthus emblica; Terminalia chebula; Terminalia bellirica. 87 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation A Study on the Pharmacological Properties of the Sundarban Mangrove Trees Based on Traditional Uses Shaila Sharmin*, Md. Niamat Hossain, Sadia Afrin, Md.Emdadul Islam, Kazi Didarul Islam, Md. Morsaline Billah Biotechnology Bangladesh. and Genetic Engineering Discipline,Khulna University,Khulna-9208, Abstract Researchers showed their interest to uncover the mystery of sundarban plants that can survive on harsh environment years after years. Based on the historical success of natural products as valuable drugs and the urgent need for new drugs, a number of the Sundarbans mangrove medicinal plants have been evaluated for their pharmacological properties. This effort has been set out to compile and appraise the results on bioactive properties of Sundarban mangrove trees based on ethnobotanical uses and also summarize the bioactive compounds identified from mangrove trees. About 27 trees have been selected due to their ample uses in different diseases. From different literature searches, it has been found that 5 trees named Barringtonia racemosa, Pongamia pinnata,Crataeva religiosa, Pandanus tectorius and Ficus sp.are mostly used by the local people of the Sundarbans area. These trees possess novel compounds like bartogenic acid, pongapinnol, lupeol, stigmasterol and serrat-3-one etc. Due to the rising incidence of resistance to synthetic antibiotics and in light of the rising costs of medicines, the research proponents of this study aims to discover most efficient drugs with the end view of providing low cost of medications and the prevention of resistance. Keywords: Pharmacological properties; ethnobotanical use; Barringtonia racemosa; bartogenic acid; lupeol. 88 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Use of Medicinal Plants against snake venom to Treat Snake Bite by Folk Medicinal Practitioners in Barandra and Shamatat region of Bangladesh Md Nasir Ahmed¹, Md Nur Kabidul Azam²*, Umme Salma Zohora ³, Md Nazmul Hasan4 ¹ Tech B Herbal Solution, Kushtia, Bangladesh. ² Proximo Biotech & Consultant, Dinajpur, Bangladesh. ³Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Saver, Dhaka, Bangladesh. 4 Deptarment of Genetic Engineering and Biotechnology, Faculty of Biological Science and Technology, Jessore Science and Technology University ,Jessore-7408, Bangladesh Abstract Snake bite is a result of an unfortunate accidental interaction between a snake and a human victim. It is the single most important toxin-related injury, causing substantial mortality in many parts of the Africa, Asia, and the Americas. A World Health Organization-funded study estimated about 8,000 cases of snakebite with over 20% mortality in Bangladesh annually. A medically important land snake in Bangladesh includes the krait, cobras, and vipers. Medically important snake species are the ones that fall into one of the three categories: bite usually causes death or disability. Snake venom is highly modified saliva (Reptile Venom Research that is produced by special glands) of certain species of snakes. The glands which secrete the zootoxin are a modification of the parotid salivary gland of other vertebrates, and are usually situated on each side of the head below and behind the eye, encapsulated in a muscular sheath. The glands have large alveoli in which venom is stored before being conveyed by a duct to the base of channeled or ubular fangs, through which it is ejected. Snake venom consists of proteins, enzymes, substances with cytotoxic effects, neurotoxins, coagulants and anti-coagulants. The objective of this study was to conduct a survey amongst the local specialists in the Barandra ( Rajshahi & Rangpur division) and Shamatat (Khulna and Part of Barisal Divission) region of Bangladesh, to collect information on plants used to treat snake bite. Local Folk Medicinal Practitioners of the study area were selected randomly and interviewed with the help of translators to gather information on the knowledge and use of plants used as a remedy for snake bite. In-depth information regarding plants type, preparation of medicines, ailments for which they are used, dosages, and side effects if any, were obtained from the local specialists. All plants were photographed, collected, identified, and vouchers were stored at the Bangladesh National Herbarium. Information on twenty plants was obtained. The collected information indicates that the following plants are used against snake venom: Justicia adhatoda L., Sansevieria trifasciata, Agaricus albolutescens, Rauwolfia canescens L., Rauwolfia serpentine (L.) Benth ex Kurz, Eclipta prostrate L. Emilica sonchifolia (L.), Anogeissus latiofolia, Cajanus cajan (L.), Clitoria ternatea L., Couroupita guianensis, Leucas aspera (Wild.), Anisomeles malabarica (L.), Wissadula periplocifolia (L.), Ceropia peltata L., Piper longum L., Morinda citrifolia L., Solanum torvum Swartz, Curcuma longa L. These medicinal plants, because of their long history of usage present considerable potential for isolation of components, which can lead to discovery of efficacious anti-venom drugs. Keywords: Alternative medicine, Snake,Snake venom, Barandra, Shamatat region. 89 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation A study based on the ethnomedicinal use of Shrubs of the Sundarbans mangrove forest Nafia Nishat, Md. Niamat Hossain, Sadia Afrin, K.M. Didarul Islam, Md. Emdadul Islam, Md Morsaline Billah* Biotechnology and Bangladesh. Genetic Engineering Discipline, Khulna University, Khulna-9208, Abstract Mangrove plants are sources of important therapeutic agents for alleviating ailments. For this reason, local people depend on these plants for treatment. Here, efforts have been made to investigate the scientific literature of some of selective shrubs on the basis of traditional uses. This report presents some selective shrubby mangrove plants such as-Acrostichum aureum, Aegiceras corniculatum, Brownlowia tersa, Clerodendrum inerme and Dalbergia spinosa which show most ethnobotanical prospects among all the shrubby mangrove plants of the Sundarbans. Most of them have antinociceptive, antidiarrhoeal analgesic, anthelmintic, anti-inflammatory, antimicrobial, antipyretic, anti-spermicidal, anti-ulcerogenic, aphrodisiac, astringent, antioxidant and larvicidal etc bioactivities. Different groups of compounds including flavonoids, steroids, alkaloids, glycosides, quinones and phenolic compounds are isolated from these mangrove plants. Moreover, this report is a compilation of information regarding traditional uses, biological activity and isolated bioactive compound obtained from data of previous assessments. Keywords: Shrubby Mangroves; Ethnobotanical potential;Bioactivity; Bioactive compound. 90 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Studies of Adulteration in Dairy Products by RP-HPLC Method Md. Shahadat Hossain1, Md. Motiur Rahman1, Subrata Bhadra1 Dilshad Noor Lira1 and Abu Shara Shamsur Rouf1,* 1 Department of Pharmaceutical Technology, University of Dhaka, Dhaka-1000, Bangladesh. Abstract This research work has been carried out to detect the presence of and if present, to quantify caffeine, preservatives and antioxidants in dairy products using reversed phase High Performance Liquid Chromatography (HPLC) method. Three different analytical methods were used to evaluate the content of caffeine, five preservatives namely benzoic acid, methyl paraben, propyl paraben, butyl paraben and formaldehyde, and two antioxidants namely Butylated Hydroxyanisole (BHA) and Butylated Hydroxytoluene (BHT). The evaluation of caffeine, benzoic acid, propyl paraben, butyl paraben, BHA and BHT was performed using a C18 column (150 mm × 4.6 mm internal diameter, 5 μm particle size) with a gradient flow rate of acetonitrile and acidic solution of sulfuric acid of pH 2.3 from ratio (%v/v) of 15:85 to 80:20 at a flow rate of 2.0 mL/min and detecting wavelength of 265 nm. The retention times of caffeine, benzoic acid, propyl paraben, butyl paraben, BHA and BHT were about 3.6, 11.6, 13.0, 13.3, 13.6 and 16.7 minutes respectively. For the evaluation of methyl paraben, the same C 18 column was used with acetonitrile and acidic solution of sulfuric acid of pH 2.3 at a ratio (%v/v) of 18:82 as mobile phase and detection was performed at wavelength of 254 nm using flow rate of 2.0 mL/min. The retention time of methyl paraben was 6.8 minutes. Formaldehyde was evaluated with a precolumn derivatization with 2,4-dinitrophenylhydrazine. The derivative was then analyzed using another C18 column (β50 mm × 4.6 mm internal diameter, 5 μm particle size) with acetonitrile and water at a ratio (%v/v) of 45:55 as mobile phase and detected at the wavelength of 345 nm. The retention time of formaldehyde was 15.8 minutes. 41 marketed dairy products of home and abroad were collected from Dhaka, Bangladesh in 2013, and then investigated. None of those products was found to be labeled to contain any of benzoic acid, methyl paraben, propyl paraben, butyl paraben, formaldehyde, BHA or BHT. Caffeine was not also labeled but six chocolate milk products were labeled to contain cocoa powder which may be the source of caffeine. 17.1% of the products were exhibited benzoic acid positive. Among these, only 14.3% of the benzoic acid positive products were approved by the Joint FAO/WHO Expert Committee on Food Additives (JECFA). 14.6% of the products showed positive response to caffeine and all were below the tolerance limit (<200 ppm) set by FDA. None of the dairy products were found to contain methyl paraben, propyl paraben, butyl paraben, formaldehyde, BHA and BHT. The amount of the total benzoic acid and caffeine were between 41 ~ 6104 ppm and 5 ~ 13 ppm respectively. Keywords: Adulteration; dairy products; RP-HPLC; caffeine, preservatives. 91 International Conference Updates on Natural Products in Medicine and Healthcare Systems Poster Presentation Determination of Caffeine and Preservatives in Energy Drinks, Soft Drinks and Malt Beverages by RP-HPLC Method Samiul Islam1, Uttom Kumar1, Subrata Bhadra1, Md. Motiur Rahman1 and Abu Shara Shamsur Rouf1,* 1 Department of Pharmaceutical Technology, University of Dhaka, Dhaka-1000, Bangladesh. Abstract A precise, sensitive and versatile reversed-phase HPLC method was developed that allowed the separation and simultaneous determination of caffeine, benzoic acid, methyl paraben, propyl paraben, butyl paraben, butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). The separations were achieved by using a gradient flow rate of acetonitrile and acidic solution of sulfuric acid (pH 2.3) from ratio (% v/v) of 15:85 to 85:15. A Capcell Pak analytical C18 column (150 mm×4.6 mm, 5 μm particle size) at a flow rate of β.0 mL/min was used and detecting wavelength was 265 nm. Under these conditions, separation of these components were achieved in less than 18 min where analytical characteristic like linearity (Caffeine: 40-200 ppm; R2= 0.992 and Benzoic acid: 50-400 ppm; R2= 0.995) was evaluated. The developed method was applied to analyze 60 marketed food and beverage products (mainly domestic), comprising energy drinks (21), soft drinks (34) and malt beverages (5). The results showed 53.33% of marketed products were found to contain caffeine, among these 15.6% of caffeine containing products exceeded the tolerance limit of caffeine (> 200 ppm) set by the United States Food and Drug Administration (US FDA). Benzoic acid was found in 65% of marketed products; among these 10.3% of benzoic acid containing products exceeded the maximum allowable limit of benzoic acid (> 600 ppm) set by Joint FAO/WHO Expert Committee on Food Additives (JECFA). Keywords: Adulteration; energy drinks; RP-HPLC; caffeine, preservatives. 92 International Conference Updates on Natural Products in Medicine and Healthcare Systems