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2019, Think India Journal
Large number of population prefer the Ayurvedic or herbal medicines to treat the various ailments. Herbal medicines are available in three forms as raw plant material or processed plant material or medicinal herbal product but mixtures have several chemical components. Therefore, it is very important to know the specific component having potential for treatment. For qualitative and quantitative analysis of the herbs and herbal drugs, Highperformance thin layer chromatography (HPTLC) is one of the best techniques. Hence, a case study has performed to quantify vasicinone component from Adhatoda zeylanica.
Think India Journal, 2019
The present work is carried out on the basis of comparison of extraction of leaf chlorophylls viz. Chlorophyll-a, Chlorophyll-b and total chlorophyll and Carotenoids from cultivated some leguminous fodder crops by using 80% Acetone as extracting method (Arnon, 1949) and the study is concerned with the concentrations of leaf pigments found between the treated and control leguminous fodder crops. Keywords:Quantitative Measurement,Spectrophotometry, Chlorophylls, Carotenoids, Leguminous Fodder crops, Extraction, Solvent.
Think India Journal, 2019
The leaf samples of the plant Cajanus cajan L. were selected for the determination of phytochemical and proximate compounds. The results procured showed the presence of compounds viz., Crude protein, crude fat, crude fibre, carbohydrates, alkaloids, flavonoids, tannins and saponin. In proximate determination the results obtained, showed the presence of crude protein 19.9%, crude fat, 3.10%, crude fibre 8.15%, NFE 61.78%. The phytochemical viz., saponin and other flavonoids have been observed. A.O.A.C (1970) Methods were followed for the determination of proximate constituents. There were variations in the concentrations of, crude protein, crude fat, crude fibre, total ash, acid insoluble ash, nitrogen free extracts.
Dept of Botany, Deogiri College , 2020
Ravi Patil, Organizing Secretary, Emerging Trans Disciplinary Approaches in Biosciences 2019 (ETDAB2019)
ETDAB 2019 Abstract Book, 2019
ETDAB2019 E Abstract, Deogiri College, Aurangabad.
Think India Journal, 2019
The aim of the present investigation was to find out the effect of leaf litter compost prepared by aerobic (NADEP) and anaerobic (Bangalore) pit methods on Nutrient content in beet root (Beta vulgaris L.). The field experiment was conducted in research farm of Dr. Babasaheb Ambedkar Marathwada University, Aurangabad. The experiment was conducted in plots of size 150 x 150 cm with six treatments and four replications. The composts were used as NADEP, Bangalore method (compost), vermicompost and Dung treatment separately in comparison with recommended fertilizer (N40:P30:K30 Kg/ha) and absolute control. The beet root was sown by the method of ridges and furrows at the seed rate of 10 Kg/ha. The Nutrient content in beet root was estimated at 70 DAS (days after sowing). The sample of beet root was kept in oven for drying, grinded to a fine powder and it was used for the estimation of Nutrient content. The leaf litter composts increased the yield and nutrient content of beet root. Beet root are biennial plants grown as annuals and harvested for their swollen root tuber. The flesh with brown/purple skin, although yellow and white fleshed varieties are also available. While beet root is most commonly eaten as a cold, salad vegetable, pickled in vinegar and it also makes a delicious hot vegetable. Grated beet root may also be eaten raw. Beet root is utilized as a food as it is rich in nutrients like protein carbohydrates, vitamins and minerals etc. In the present investigation, application of cattle dung, NADEP compost and vermicompost increase total reducing sugar in the beet root as well as total plant. While significant reduction was observed after the application of fertilizer and many times it was less than the control. Leaf litter can be considered as a source of Agricultural waste which return directly to the soil after the formation of compost.
Think India Journal, 2019
ABSTRACT In this study, tuber extracts of Arisaema tortuosum were investigated for in-vitro antibacterial and antioxidant activity. The antibacterial properties were tested against five human pathogens such as Klebsiella pneumonae, Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Pseudomonas aeriginosa using two solvents viz methanol and acetone by well diffusion method. The maximum antibacterial activity was observed in methanolic extract against all tested pathogenic strains. The antioxidant potential of Arisaema tortuosum was determined by using three methods viz DPPH, HRSA and FRAP assay. The results obtained from these three methods revealed % free radical scavenging activity in methanolic extract. In FRAP, methanolic extract shown higher antioxidant power than that of acetone extract. The standard antioxidant BHA at the same concentration exhibited a good deal of free radical scavenging percentage.
The present paper deals with development and standardization of HPTLC method used for quantification of vasicine and vasicinone in Adhatoda vasica commonly known as Vasaka, a plant of the family Acanthaceae. Today, vasaka is the most widely known herb and widely used in the treatment for a variety of respiratory ailments including cough, bronchitis and asthma. Both vasicine and vasicinone are two major alkaloids of A. vasica, which are known to possess biological activities like respiratory stimulant, bronchodilator and hypotensive activity. Hence, a need to develop and standardize a chromatographic method for quantification of vasicine and vasicinone, for better standardization of Adhatoda vasica. An attempt has been made to quantify vasicine and vasicinone in A. vasica raw herbs, extracts and formulations by HPTLC method. The lowest detectable limit of vasicine and vasicinone was found up to 1 ng and 25 ng respectively with good resolution and separation of vasicine and vasicinone from other constituents of A. vasica. Further, recovery values of vasicine and vasicinone were found to be about 95-102 %, which shows the reliability and suitability of the method. The validated HPTLC method was found to be simple, reproducible, accurate and precise. The structure of isolated vasicine and vasicinone was characterized and confirmed by various advanced spectroscopic methods.
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