(PDF) Biofortification: an approach to eradicate micronutrient deficiency

Micronutrient deficiency also known as "hidden hunger" refers to a condition that occurs when the body lacks essential vitamins and minerals that are required in small amounts for proper growth, development and overall health. These deficiencies are particularly common in developing countries, where a lack of access to a varied and nutritious diet makes it difficult for people to get the micronutrients they need. Micronutrient supplementation has been a topic of interest, especially during the Covid-19 pandemic, due to its potential role in supporting immune function and overall health. Iron (Fe), zinc (Zn), iodine (I), and selenium (Se) deficiency in humans are significant food-related issues worldwide. Biofortification is a sustainable strategy that has been developed to address micronutrient deficiencies by increasing the levels of essential vitamins and minerals in staple crops that are widely consumed by people in affected communities. There are a number of agricultural techniques for biofortification, including selective breeding of crops to have higher levels of specific nutrients, agronomic approach using fertilizers and other inputs to increase nutrient uptake by crops and transgenic approach. The agronomic approach offers a temporary but speedy solution while the genetic approach (breeding and transgenic) is the long-term solution but requires time to develop a nutrient-rich variety.

TYPE Review 14 September 2023 10.3389/fnut.2023.1233070 PUBLISHED DOI OPEN ACCESS EDITED BY Monika Thakur, Amity University, India REVIEWED BY Saloni Sharma, National Agri-Food Biotechnology Institute, India Neetu Singh, Amity Centre for Biocontrol and Plant Disease Management Amity University Noida U.P, India Biofortification: an approach to eradicate micronutrient deficiency Avnee 1, Sonia Sood 2, Desh Raj Chaudhary 2, Pooja Jhorar 1 and Ranbir Singh Rana 1* 1 Department of Agronomy, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, India, Department of Vegetable Science and Floriculture, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, India 2 *CORRESPONDENCE Ranbir Singh Rana ranars66@gmail.com RECEIVED 01 June 2023 August 2023 PUBLISHED 14 September 2023 ACCEPTED 21 CITATION Avnee, Sood S, Chaudhary DR, Jhorar P and Rana RS (2023) Biofortification: an approach to eradicate micronutrient deficiency. Front. Nutr. 10:1233070. doi: 10.3389/fnut.2023.1233070 COPYRIGHT © 2023 Avnee, Sood, Chaudhary, Jhorar and Rana. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Micronutrient deficiency also known as “hidden hunger” refers to a condition that occurs when the body lacks essential vitamins and minerals that are required in small amounts for proper growth, development and overall health. These deficiencies are particularly common in developing countries, where a lack of access to a varied and nutritious diet makes it difficult for people to get the micronutrients they need. Micronutrient supplementation has been a topic of interest, especially during the Covid-19 pandemic, due to its potential role in supporting immune function and overall health. Iron (Fe), zinc (Zn), iodine (I), and selenium (Se) deficiency in humans are significant food-related issues worldwide. Biofortification is a sustainable strategy that has been developed to address micronutrient deficiencies by increasing the levels of essential vitamins and minerals in staple crops that are widely consumed by people in affected communities. There are a number of agricultural techniques for biofortification, including selective breeding of crops to have higher levels of specific nutrients, agronomic approach using fertilizers and other inputs to increase nutrient uptake by crops and transgenic approach. The agronomic approach offers a temporary but speedy solution while the genetic approach (breeding and transgenic) is the long-term solution but requires time to develop a nutrient-rich variety. KEYWORDS biofortification, hidden hunger, selective breeding, sustainable, vitamins and minerals Introduction Over 2 billion people worldwide suffer from micronutrient deficiency which has a negative impact on their health and socio-economic condition (1). The principal reason is the consumption of cereal-based food which provide enough calories but they are deficient in phytochemicals (minerals, vitamins, antioxidants, and fiber). These phytochemicals are essential for the normal growth and development of humans and their deficiencies can have Frontiers in Nutrition 01 frontiersin.org Avnee et al. 10.3389/fnut.2023.1233070 reduce malnutrition and improve public health, particularly in populations that rely heavily on a single staple crop for their daily caloric intake. It requires a one-time investment unlike supplements, reach malnourished poor population and provide better quality food without compromising yield (see Figure 2). This can be particularly important in developing countries where micronutrient deficiencies are common and can have serious health consequences. Iron, zinc, iodine and selenium deficiencies are the most common, which account for around 60% of iron, 30% of zinc and iodine and 15% of selenium deficiency (3). However, biofortification alone is not enough to eradicate malnutrition. It cannot provide such a high level of nutrients as through supplements or fortified food but they improve daily dietary intake of nutrients (4). In the context of climate change also, the anticipated drop in dietary micronutrients makes biofortification more important for vulnerable groups to maintain good health. The concept of simultaneously biofortifying crops with multiple essential micronutrients is an innovative and promising strategy to address widespread nutrient deficiencies and improve overall human nutrition. This approach, often referred to as “multi-nutrient biofortification” or “combinatorial biofortification,” aims to create crops that contain a balanced array of various essential vitamins and minerals. This strategy can provide a more holistic and comprehensive approach to combating malnutrition by addressing multiple nutrient deficiencies concurrently. By combining different nutrients in crops, their overall bioavailability and health benefits can be maximized. Multi-nutrient biofortified crops can encourage dietary diversity as people consume a wider range of nutrients from staple foods. In addition to cost savings from development to distribution and synergies through aggregated health effects, multi-nutrient biofortification can result in significantly higher market coverage by preventing competition between numerous single-nutrient biofortified varieties (5). 70.00% 60.00% 50.00% 40.00% 30.00% 20.00% 10.00% 0.00% Central African Republic North Korea Uganda Iraq Afghanistan Kenya Ethiopia Sudan Pakistan Bangladesh India Nigeria Philippines Myanmar Peru Sri Lanka Nepal China South Africa Iran United Arab Emirates percent population affected by malnutrition serious health consequences, including diminished cognitive degeneration in children, increased risk of infections and a range of other negative effects on physical and mental health. Green Revolution, which took place from the mid-20th century onwards, marked a significant shift in agricultural practices and policies, particularly in developing countries like India. During the revolution, the emphasis was shifted on boosting crop productivity, notably that of rice and wheat, which led to the domination of these two crops in the nation. The increased productivity ensured food security in the country but on the other hand, decreased bio-diversity resulted in a monotonous cereal-based diet and thus increased concern about nutritional security. The ever-growing population of India further worsens the problem of providing sufficient nutrients to all. Over 21.9% of the Indian population is living in extreme poverty with limited access to resources (2). With their poor purchasing power, they consume what they produce in their fields. In order to alleviate malnutrition and to attain nutritional security in the country, a second green revolution is therefore required, with a particular emphasis on the development of biofortified, nutrient-rich varieties. Staple crops, such as rice, wheat and maize are the main source of calories for a large proportion of the world’s population, particularly in low-income countries. These crops, however, often lack essential vitamins and minerals, which might result in micronutrient deficiency. Biofortification can help to address this problem more sustainably and economically by increasing the levels of essential vitamins and minerals in staple crops. It is the process of enrichment of bio-available concentration in edible portions of food and aims at providing nutrient-rich food to rural resource-poor people who do not have access to diversified food, supplements and industrially fortified food (see Figure 1). Biofortification can be a costeffective and sustainable way to address micronutrient malnutrition at the population level with an ultimate goal to Countries FIGURE 1 Different countries and their percentage of population suffering from malnutrition. Frontiers in Nutrition 02 frontiersin.org Avnee et al. 10.3389/fnut.2023.1233070 Approaches of biofortification promising avenue for increasing the concentration and bioavailability of essential nutrients in food crops, a strategy known as “nanofertilizer-assisted biofortification.” Because of the large increase in surface area and the NFs’ small size, plants can easily absorb the particles (8). Iron biofortification Iron deficiency is a common problem, particularly in developing countries and it can have serious health consequences such as anemia affecting over half the population of children under the age of five and pregnant women in India. Biofortification can help to address this issue by increasing the iron content of crops, which can in turn help to improve the iron intake of individuals who rely on these crops as a dietary staple. Iron is a vital nutrient for humans, it is required for proper body functioning but cannot be produced by the body and must be obtained from the diet. It is crucial for the production of red blood cells and the transportation of oxygen within the human body, supporting the immune system, providing energy and maintaining healthy skin, hair and nails. It is especially important for pregnant women, infants, and young children, as they have higher iron requirements. Anemia, weariness, and immune system impairment are just a few of the health issues that iron deficiency can cause, especially in impoverished nations where plant-based foods are the main source of Fe (9). Applying iron-rich fertilizers to the soil and proper soil management, such as maintaining the pH and nutrient balance of the soil can help to improve the uptake of iron by crops. It is important to note that the efficacy of these methods can vary based on the particular crop and growing circumstances. Comparing vegans to meat-eaters, the former should consume 1.8 times more of the recommended daily intake (RDA) of Iron than later (10). Table 1 shows some of the crops that are successfully agronomically biofortified with iron. FIGURE 2 Methods of biofortification (Agronomic, Breeding and Transgenic). Agronomic biofortification Agronomic biofortification refers to the process of enriching the nutritional value of crops through fertilization and soil management. It offers an efficient and timely solution that is the quickest and most affordable way to produce nutrient-dense food, albeit it only offers a short-term fix. The majority of crops can benefit from this very simple method of biofortifying with iron, zinc, iodine, and selenium. To boost the content of micronutrients in the plant’s edible parts, micronutrientcontaining organic/inorganic fertilizers or biofertilizers are applied to the plant by foliar or soil application. Micronutrient concentration depends on the source of fertilizer used, method and rate of fertilizer application, stage of application, and translocation of nutrients within plants. Due to variations in mineral mobility, mineral accumulation among plant species and soil compositions in the particular geographic region of each crop, the success of agronomical biofortification is highly variable (6). The effectiveness of agronomic biofortification has increased with the development of high specialized fertilizers with high nutrient uptake efficiency and greater nutrient translocation to the consumable sections of a crop plant, which include water-soluble fertilizers, chelated fertilizers and nanofertilizers (7). Nanoparticles, which are extremely small particles with unique properties due to their size and structure, have gained attention as potential tools for biofortification. Nanofertilizers (NFs), a subset of nanotechnology applications in agriculture, hold significant potential to revolutionize traditional methods of enhancing crop nutrient content and improving overall nutritional quality. By harnessing the unique properties of nanoparticles, NFs offer a Frontiers in Nutrition Zinc biofortification Zinc is a crucial micronutrient that is necessary for both plants and humans. It is involved in a wide range of physiological functions including immune response, protein and DNA synthesis, wound healing and involved in the metabolism of carbohydrates, proteins and fats. Zinc deficiency is a major public health problem affecting around 30% of the world’s population, making people more susceptible to issues including maternal mortality, DNA damage, growth retardation, changes in taste and smell, immunological dysfunction, and an increased risk of infections (23). Biofortification can assist to solve the issue of zinc deficiency and improve the nutritional value of crops, particularly in areas where deficiency is prevalent. Zinc deficiency in humans and soil show geographical overlap (24). A high percentage of agricultural land (36.5%) in India is zinc deficient and cultivating crops on these deficient soils further reduces zinc levels in edible portions (25). The deficit is particularly prevalent in low-income developing nations where a plant-based diet is the norm (26). Agronomic biofortification of zinc was reported to be successful in a large number of crops (Table 2). 03 frontiersin.org Avnee et al. 10.3389/fnut.2023.1233070 TABLE 1 Agronomic biofortification of iron. Crop Treatment References Cereals Rice Foliar application of FeSO₄ sprayed @ 0.2% at panicle initiation stage, 7 days after flowering (11) (DAF), 14 DAF Brown rice 0.5 and 1.0% FeSO4. 7H2O at maximum tillering, pre-anthesis and post-anthesis stages (12) Aerobic rice Soil application of 67 mg FeSO₄.7H₂O per kg soil at the time of sowing; three foliar sprays (at (13) 40, 60 and 75 DAS) of 3% FeSO₄.7H₂O solution and 0.05 M Fe-EDTA used as seed treatment Wheat Three foliar sprays of FeSO₄ at tillering, booting and heading stage (14) Foliar application of Fe3O4 nanofertilizer (5 mg L ) (15) Foliar application of 0.5, 1 and 1.5% solutions of FeSO4 at branching and flowering stages. (16) −1 Pulses Mungbean Increased Fe concentration by 46% in grains. Cowpea Four foliar application rates (0, 25, 50 and 100 μM L−1) each of iron chelates and ferrous (17) sulfate. Increased Fe concentration by 29–32%. Chickpea Foliar application of FeSO4.7H2O resulting in an increased grain concentration by 21–22% (18) Lentil Foliar spray of 0.5% FeSO4.7H2O at the pre-flowering stage. (19) Increase in concentration by 17.4 mg kg−1 in grains. Vegetables Potato Soil (Amino acid-based Fe complex) and foliar applied EDTA chelated Fe (20) Red and green pigmented Lettuce Soilless culture: Fe at conc. 0.5, 1.0 and 2.0 mM iron (21) Brassicaceae microgreens (Arugula, red cabbage, Soilless media: Fe conc. 0, 10, 20, 40 mg L−1 (22) and red mustard) Iodine biofortification Selenium biofortification Iodine is a necessary component of human metabolism and crucial for the proper function of the thyroid gland in humans, energy production and body temperature regulation, despite not being a necessary element for plants, although its application has been linked to higher yields and high iodine content in several crops (Table 3). It is estimated that the iodine intake of 30–38% of people worldwide is insufficient (45). Iodine deficiency can lead to a variety of health problems, including goiter, infertility, growth impairment, hypothyroidism and intellectual disability. Iodine biofortification can be a particularly useful approach in areas where iodine deficiency is common and people rely heavily on plant-based foods as a source of nutrients. Biofortified plantbased foods can help to increase the overall iodine intake of a population and improve the nutritional status of individuals who consume these foods. Adults should consume between 150 to 290 μg of iodine per day, with a tolerable upper limit of 1,100 μg per day (53, 54). One of the most common ways to fortify iodine is through the addition of iodine to salt. This is called iodized salt. Iodized salt may not be effective in preventing iodine deficiency in all populations as it can raise blood pressure which is a major risk factor for heart disease and stroke. It is also linked to an increase in cases of osteoporosis, a condition that causes the bones to become weak and brittle. A promising strategy to raise the iodine content of crops is agronomic biofortification. Selenium is a trace element that is essential for human health. Due to its incorporation into selenoproteins like glutathione peroxidase, which perform a number of functions including antioxidant activity, it is essential for the immune system’s proper operation (55). Se deficiency affects hundreds of millions of people around the world (56). For plants, Se is not essential (57, 58), but when applied at low doses, it is beneficial for some groups of plants by increasing the activity of various enzyme systems; selenium alone or in combination with iodine was found to increase concentration, better quality in some plants (Table 4); for example, it delays tomato fruit ripening by inhibiting ethylene biosynthesis and enhancing the antioxidant defense system (80). Plants can absorb selenium from the soil, but the availability of selenium in the soil can vary widely. In some areas, the soil may be naturally low in selenium, while in others, selenium may be present but not in a form that plants can easily absorb. To ensure that plants are getting sufficient selenium, it may be necessary to add selenium to the soil. This can be done through the use of selenium fertilizers or through the application of selenium-rich compost or manure. It is also possible to provide selenium to plants through the use of selenium-rich irrigation water or through the use of selenium-enriched seeds. Frontiers in Nutrition Interaction among nutrients The effectiveness of agronomic biofortification can be affected by interactions between macronutrients and micronutrients (81) (see 04 frontiersin.org Avnee et al. 10.3389/fnut.2023.1233070 TABLE 2 Agronomic biofortification of zinc. Crop Treatment References Soil application of ZnSO₄.7H₂O @ 2.5, 5 and 7.5 kg/ha (27) Cereals Maize Aromatic rice Soil and foliar application (at flowering) of zinc as ZnSO₄.7H₂O (28) Basmati rice Different Zn sources applied at the rate 5 kg Zn/ha Singh and Shivay (29) Rice Soil and foliar sprays (two) at maximum tillering and panicle initiation stage with different (30) sources of zinc at different rates Wheat Soil and foliar application of ZnSO₄.7H₂O and zinc-coated urea Prasad and Shivay (31) Soil and foliar sprays (two) at maximum tillering and booting stage with different sources (32) of zinc at different rates Soil and foliar application of zinc as ZnSO₄.H₂O (33) Dextran sulfate (DEX (SO4))-coated ZnO Nanoparticles Elhaj and Unrine (34) Different rates and source of Zinc at 1/3- sowing, 1/3–3 weeks after sowing (WAS) and Prasad and Shivay (31) 1/3–6 WAS Bread wheat, durum wheat and triticale Foliar application of 0.5% ZnSO₄.7H₂O at maximum tillering, flower initiation, milk and (35) dough stages. The maximum percent increase was 145.9% in wheat, 178.1% in durum wheat and 157.4% in triticale Pulses Chickpea Soil application (5 kg/ha) through ZnSO4.7H2O (36) Increase in concentration of zinc by 5.4 mg kg−1 Pigeon pea Soil application (20 kg/ha) ZnSO4.7H2O Behera et al. (37) Increase in concentration of zinc by 10.6 mg kg−1 Lentil Foliar spray of 0.5% ZnSO4.7H2O at the pre-flowering stage. (19) Increase in concentration by 10.5 mg kg−1 in grains. Oilseeds Safflower Linseed Soil application (5 kg/ha) through ZnSO4.7H2O Roy and Ghosh (38) Foliar application of ZnSO4.7H2O and Zn-EDTA (0, 0.25, 0.50, and 0.75%) at flowering (39) and capsule formation stages Vegetables Brassicaceae microgreens (Arugula, red cabbage, Soilless media: ZnSO4 (5–10 mg/L) and red mustard) 75–281% increase in zinc content in crop Arugula (Broad leaf) (22) Foliar application (Zn @ 1.5 kg/ha at 25 DAE) (40) 279% increase in Zn content Lettuce Different Zn doses (0,5, 10, 20, 30 mg kg−1) (41) Broccoli 0.25% ZnSO4. 7 H2O @ 15 mL per pot (42) Pear Foliar spray of 1.5% ZnEDTA (43) Banana Injection of ZnSO4. 7 H2O in pseudostem @ 1, 2 and 4% (44) Fruits Triple zinc content in fruit than control Table 5). While previous biofortification initiatives have mainly concentrated on increasing specific nutrients, a novel strategy could be to simultaneously biofortify crops with a number of essential micronutrients, providing a more comprehensive nutritional profile. Some nutrients work synergistically in the body, enhancing the absorption and utilization of others. Zinc, for example, improves nitrogen metabolism by promoting effective uptake and assimilation. It also aids in the conversion of phosphorus into forms that are easily absorbed by plants, as well as in the regulation of stomatal function Frontiers in Nutrition and water movement, both of which affect potassium absorption. The synthesis of selenium-containing phytochemicals, such as selenocompounds can be improved by selenium biofortification. According to (82, 83), plants convert selenium into selenoamino acids, which are then converted into phytochemicals. These substances have antioxidant qualities beneficial to human health. Selenium also plays a role in reducing element toxicity and regulating the concentration of micronutrients in plants by modifying soil conditions, encouraging microbial activity, taking part in crucial physiological and metabolic 05 frontiersin.org Avnee et al. 10.3389/fnut.2023.1233070 TABLE 3 Agronomic biofortification of iodine. Crop Treatment References Tomato Potassium iodide (KI), KIO3 + SA, KI + SA @ I-7.88 μM and 7.24 μM (45) Pepper KI at 0.25 to 1 mg/L (46) Increased iodine content by 350–1,330 μg/kg Cabbage and cowpea Potassium iodide @ 15 kg I /ha (47) Increase in iodine conc. to 109.1 mg/kg in cabbage and 5854.2 mg/kg in cowpea Carrot Iodine dose at 0.5 mg/L (48) Potato KIO3 @ 2.0 kg iodine per hectare (49) Apple and Pear Foliar application of KI and KIO3 (0.5, 1.0 and 2.5%) (50) Leafy greens Soil and foliar Iodine application at different concentrations (0, 5, 10 kg/ha) (51) KI @10 μM (52) (Rapeseed and Amaranthus) Sweet basil and lettuce processes, generating element competition, stimulating metal chelation, organelle compartmentalization and sequestration (84). It is preferable to combine Zn, Se, and Fe in conjunction with the employment of plant growth-promoting bacteria (PGPR) and arbuscular mycorrhizal fungus (AMF), in order to develop biofertilizers that are ecologically benign yet result in crops that are enriched in microelements (85). In some cases, certain nutrients can compete with each other for absorption. By ensuring an adequate balance of multiple nutrients, competition for absorption can be minimized. Adequate levels of nutrients like magnesium, zinc and manganese are important for vitamin C synthesis. These nutrients can impact the enzyme systems involved in ascorbic acid production. Certain micronutrients, such as copper and manganese, are cofactors for enzymes involved in the synthesis of antioxidants and flavonoids (86). Adequate levels of these micronutrients can positively influence the production of these compounds. The formation of roots, the movement of shoots and the re-localization of nutrients from vegetative tissue to the seeds are all positively impacted by a plant’s N and P status. As a result, the crop’s edible sections absorb more micronutrients and have higher concentrations of them (87). Salt, high/low temperature, heavy metals, and drought all cause the overproduction of reactive oxygen species (ROS) and the induction of oxidative stress in plants (85). It has been demonstrated that biofortification with Zn, Se, and Fe using various types and forms of fertilizer can reduce the damage caused by oxidative stress by increasing the content of ROS-scavenging enzymes such as superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), glutathione peroxidase (GPX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione S-transferase (GST), and peroxiredoxin (PRX) content in different sites of plant cells (88, 89). involves selecting plants with desirable traits, such as higher micronutrient content and crossing them with other plants to create a hybrid with improved characteristics. Over time, this process is repeated and the offspring are screened for desired traits. Plant breeding techniques are used in the biofortification approach to develop staple food crops with greater micronutrient content (97), this assists to target low-income households in the country. Numerous crops have been targeted for biofortification through crop breeding due to their improved acceptance (Table 6). A biofortified crop system is highly sustainable. Nutritionally improved varieties will continue to be grown and consumed year after year, even if government attention and international funding for micronutrient issues fade (105). Since the last four decades, yield qualities and resistance breeding have received the majority of attention resulting in lower amounts of nutrients in the existing varieties (106). Biofortification by breeding is attained when crops have naturally some concentrations of micronutrients, such as iron, zinc and vitamin A, which means when genetic diversity is accessible in usable form. Some examples of biofortified crops include iron-rich beans, zinc-rich rice, selenium-rich rice, wheat and maize, iodine-rich cassava, maize, and sweet potatoes and vitamin A-rich sweet potatoes. This method is widely accepted as it is safe and does not raise the same safety concerns as genetic engineering. However, traditional breeding can be a slow and labor-intensive process, and it may take many years to develop a crop with improved nutrient content. Target crops for biofortification Cereals Rice, wheat and maize are the major calorie supplement for two-thirds of the Indian population thereby ruling the people’s diet in the country. Biofortification of cereals with iron, zinc, protein and provitamin-A content can assist to bring down the issue of hidden hunger in the population who does not have access to diversified food or supplements. Cereals are typically deficient in both protein and vitamin A. Proteins are vital for humans as they build cells, act as enzymes for chemical reactions, regulate hormones, support the immune system, aid in muscle function, and facilitate communication Biofortification through conventional breeding Traditional plant breeding is a method of improving the nutritional content of crops by selecting for desired traits through controlled crosses between different plant varieties. The process Frontiers in Nutrition 06 frontiersin.org Avnee et al. 10.3389/fnut.2023.1233070 TABLE 4 Agronomic biofortification of selenium. Crop Targeted micro-nutrient Treatment References Pulses Chickpea Selenium Two foliar Se fertilizers (sodium selenate and sodium selenite) at four rates (59) (0, 10, 20, 40 g ha−1) Soybean Selenium Lentil Selenium Foliar application of sodium selenite and Se-enriched fertilizer (60) Foliar application of 40 g/ha of Se as potassium selenate (K2SeO4) (10 g/ha (61) during full bloom and 30 g/ha during the flat pod stage) Increased seed Se concentration from 201 to 2,772 μg/kg Oilseeds Mustard Selenium Accumulation of 358 mg kg−1 in seed (62) Carrot and Broccoli Selenium Selenium conc. at 1.65 mg/kg, 0.92 mg/kg and 88 μg/L, 48.6 μg/L (63) Carrot Iodine and selenium KI + Na2SeO3 & KIO3 + Na2SeO3 (64) Turnip Selenium Selenite at 50 to 100 mg L Radish Selenium Selenate and selenite @ 20 μmol L (66) Carrot Iodine and selenium KI + Na2SeO4 (4 kg I /ha: 0.25 kg /ha) (67) Root crops (65) −1 −1 Iodine and selenium content (7.7 and 4.9 times) Carrot Iodine and selenium KI and Na2 SeO4 (68) (4 kg I ha−1: 0.25 kg Se ha−1) Cole crops Broccoli Selenium Se dose, selenite and selenate (69) Cabbage Selenium Na2O4Se: betaine: adjuvant (10 μM: 10 μM: 1%) (70) Broccoli and Carrot Selenium Selenium enriched S. pinnata (soil amendment) (71) Broccoli Selenium Sodium selenate (50 μM) (72) Mustard sprout Selenium Selenate and selenite @ 20 μmol L−1 (66) Cabbage Selenium 8 mg kg and 16 mg kg Se yeast (73) Selenium and iodine Selenium and iodine combination (74) Lettuce Selenium Selenate at 40 μmol L−1 (75) Lettuce Selenium Selenate application at low concentration (58) Lettuce Iodine and selenium Foliar application of Na2SeO4 and Na2SeO4 + KIO3 and for iodine content (76) −1 −1 Cucurbits crops Pumpkin Leafy vegetables in roots KIO3 and Na2SeO4 + KIO3 Solanaceous vegetables Tomato Selenium Selenium dose at 10 mg L−1 (77) Tomato Selenium Sodium selenite at 5 mg L−1 (78) Accelerated accumulation of selenium by 53% Cherry tomato Selenium Selenium at 2.0–4.0 μmol L−1 + grafting (soilless media) Se concentration (79) (9.8 mg kg−1) between cells, among other essential roles in maintaining overall health and bodily functions. Dietary protein deficiency can lead to varied effects on body weight and composition. Inadequate protein intake often results in increased food consumption, body weight, and fat mass. Extremely low protein diets cause fatty liver, reduced energy absorption, and persistent decreases in lean mass (107). Vitamin A aids in cell communication, supports reproduction and growth, and acts as an antioxidant to protect cells. Its deficiency can cause problems Frontiers in Nutrition in the eyes (ophthalmological), skin (dermatological), and immune system (immune impairment) (108). Polished rice is a poor source of micronutrients; 60–80% of Fe and around 30% of Zn are lost during polishing (109) yet consumers prefer polished rice because of their long storage and their taste preference. Pureline varieties of rice developed by ICAR-IIRR are DRR Dhan 45, DRR Dhan 48 and DRR Dhan 49 having zinc content ranging from 22.6–25.2 ppm. Protein-rich variety CR Dhan 310 has 07 frontiersin.org Avnee et al. 10.3389/fnut.2023.1233070 TABLE 5 Effect of nutrients on other nutrients and phytochemicals. Crop Targeted nutrient Treatment Improved traits References Macro (N, P, K, Ca, and Mg) and Zn Chelate and Zn Sulphate Antioxidant activity and macro and (90) Micronutrients (Fe, Mn, Zn, Cu, and Ni) (25 to 50 μM) micro-nutrient content Borax (0.5 and 1%) and Increased oil and protein content (91) 8 mg kg−1 and 16 mg kg−1 Se Antioxidant activity and nutritional (73) yeast quality (ascorbic acid, soluble sugar, free Legume crops Green beans Oilseeds Mustard Boron and nitrogen urea (1%) Cole crops Cabbage Selenium amino acids, SOD activity, Glucosinolates, and Phenolic compounds) Broccoli Nitrogen and Zinc 0.25 + 0.25 of N and Zn Antioxidant activity, Zn (more than (foliar application) 50 mg K−1) and total phenol content (92) Potassium sulphate at Potassium content (93) PSB + FYM applied at Micronutrients (Ca, Mg, Zn and Fe), (94) different stages vitamin C and beta-carotene Soil and foliar application of Phenolic compounds (95) Anthocyanin concentration (66) Ascorbic acid (46) Ascorbic acid, soluble sugar, chlorophyll-a (67) Cucurbits crops Cucumber Potassium 0.014–4 g L−1 Leafy vegetables Spinach Lamb’s Lettuce PSB Selenium Selenium Alfalfa Selenium Selenate and selenite @ 20 μmol L−1 Solanaceous vegetables Pepper Iodine Hydroponic experiment: KI conc. 0.25–5 mg L−1 Tomato Selenium Selenium dose at 10 mg L−1 content, peroxidase, catalase, and superoxidase dismutase Tomato Selenium Sodium selenite at 2 mg L Selenium Selenite at 50 to 100 mg L−1 −1 Biosynthesis of phytochemical compound (96) Selenium content and other minerals viz., (65) Root vegetable Turnip Mg, P, Zn, Mn, and Cu. 10.6% protein content in polished rice in comparison to 7.0–8.0% in popular varieties. Wheat is the second main crop of India after rice. Breeding wheat to improve the quality of the crop has become the recent focus. However, farmers’ acceptance of nutrient-dense cultivars and the introduction of new biofortified varieties into wheat-growing areas is crucial in the fight against hidden hunger (110, 111). Although iron and zinc are abundantly present in the aleurone layer, however, their bioavailability is affected by the presence of phytate (112). Supplementing with vitamin A presents a problem due to its high cost and need for efficient transportation and storage methods, which are challenging to implement in remote, sparsely populated locations (113). Maize is an ideal crop for biofortifying it with provitamin A due to its natural diversity in carotenoid content, which includes predominant carotenoid components lutein and zeaxanthin, as well as β & α-carotene and β-cryptoxanthin (114). Traditional maize contains less amount of proteins, lysine and tryptophan, over-dependency on Frontiers in Nutrition this cereal causes diseases such as kwashiorkor and pellagra (115, 116). There are presently several varieties of quality protein maize (QPM) being grown throughout the country having high lysine and tryptophan levels. Pulses Pulses are a major source of protein and other vital nutrients for millions of people, especially in developing countries. Biofortification can effectively address malnutrition by providing more of the key nutrients needed for proper growth, development and overall health. Pulses are not only nutritionally valuable but also environmentally beneficial, as they have the ability to fix nitrogen in the soil, enhancing its fertility. Lentils and beans are particularly promising candidates for enhancing iron and zinc content through conventional breeding methods. These pulses exhibit inherent genetic potential for heightened 08 frontiersin.org Avnee et al. 10.3389/fnut.2023.1233070 TABLE 6 Varieties developed through conventional breeding. Crop Variety Trait Country Year release References DRR Dhan 45 Zn India 2016 (98) DRR Dhan 48, DRR Dhan 49 Saurbhi Zn India 2017 (98) Zn India 2017 (98) Fedearroz BIOZn 035 Zn Colombia 2021 (99) Inpara 11 Siam HiZInc Zn Indonesia 2022 (99) BRRI Dhan 64 Zn Bangladesh 2014 (99) Cereals Rice Wheat Maize CR 310, CR 311 Protein India 2018 (100) Zinco Rice MS Zn India 2018 (101) WB 02, HPBW 01 Fe, Zn India 2017 (102) Pusa Tejas Protein, Fe India 2017 (102) Pusa Ujala Protein, Fe, Zn India 2017 (102) HD 3171 Zn India 2017 (102) Nohely F2018 Zn Mexico 2018 (99) TARNAB-REHBAR Zn Pakistan 2023 (99) TARNAB-GANDUM-I Zn Pakistan 2023 (99) Zinc Gahun-1 Zn Nepal 2020 (99) HI 8777 Zn, Fe India 2018 (102) MACS 4028 Protein, Zn and Fe India 2018 (103) PBW 752 Protein India 2018 (103) Vivek QPM 9 lysine and tryptophan India 2008 (102) Pusa HM8 Improved, Pusa HM4 lysine and tryptophan India 2017 (102) Pusa Vivek QPM9 Improved provitamin-A, lysine and tryptophan India 2017 (102) ZS246A Vitamin A Africa 2016 (99) ZS500A Vitamin A Africa 2019 (99) ICTA HB-18ACP + Zn Zinc Guatemala 2018 (99) Fortaleza 17 Zinc Guatemala 2020 (99) SGBIOH2 Zinc Colombia 2019 (99) Pusa HQPM 5 Improved, Pusa HQPM 7 provitamin-A, lysine and tryptophan India 2020 (2) RHB 233, RHB 234 Iron and zinc India 2019 (103) ICSR 14001, ICSH 14002 Iron India VR 929 Iron India 2020 (2) LCIC MV5 Iron Nigeria 2023 (99) Chakti Iron Nigeria 2018 (99) Improved, IQMH 201 Pearl millet Sorghum Finger Millet Little Millet (104) CFMV1, CFMV 2 Calcium, iron and zinc India 2020 (2) CLMV1 Iron and zinc India 2020 (2) Parbhani Shakti Zinc India 2018 (99) Pusa Ageti Masoor Iron India 2017 (102) IPL 220 Iron and zinc India 2018 (103) IPL 220 Iron and zinc India 2018 (103) Kufri Manik, Kufri Neelkanth Anthocyanin India 2020 (2) Pulses/Legumes Lentil Bhu Sona, Bhu Krishna Provitamin-A, Anthocyanin India 2017 (102) Rasuwa black Iron Nepal 2020 (99) Barimasur-4, B-5, B-6 Iron Bangladesh 2010 (99) (Continued) Frontiers in Nutrition 09 frontiersin.org Avnee et al. 10.3389/fnut.2023.1233070 TABLE 6 (Continued) Crop Variety Trait Country Cowpea CBC6 Iron Zimbabwe 2021 (99) Pant Lobia-7 Iron India 2019 (99) Beans Year release References BRS Araca Iron Brazil 2009 (99) RWR 2245; RWR 2154; MAC 42; MAC 44; Iron and zinc Rwanda 2014 (99) Delvia Vitamin A Zimbabwe 2021 (99) Kokota, Chumfwa, Olympia Vitamin A Zambia 2014 (99) CAB 2; RWV 1129; RWV 3006; RWV 3316; RWV 3317; RWV 2887 Vegetables Sweet Potato Cassava Gerald, Joweria Vitamin A Uganda 2013 (99) Slicass 12 Vitamin A Sierra Leone 2014 (99) UMUCASS 44 Vitamin A Nigeria 2014 (99) UMUCASS 52, UMUCASS 53, UMUCASS 54 Vitamin A Nigeria 2022 (99) Fruits Banana Apantu, Bira, Pelipita, Lai, To’o Vitamin A Uganda Mango Amarpali, Pusa Arunima, Pusa Surya, Pusa Beta-carotene, Vitamin C India Ataulfo Beta-carotene, Vitamin C Mexico Pusa Navrang Antioxidants India (99) IARI, India Pratibha, Pusa Peetamber, Pusa Lalima, and Pusa Shreshth Grapes mineral accumulation. Harnessing this natural richness entails meticulous selection of parental genotypes demonstrating superior mineral concentrations. Through systematic interbreeding over successive generations, novel cultivars can be developed wherein augmented iron and zinc levels are seamlessly integrated while preserving key agronomic attributes. Consequently, these biofortified varieties emerge as pivotal assets for ameliorating micronutrient deficiencies and fostering enhanced nutritional quality within food systems. IARI, India treatments for diseases and enhancing the nutritional quality of crops. Biotechnology allows more precise and efficient targeting of specific nutrients than other means. Nutritional quality in crops can be enhanced either by adding new genes that supply the plants with more vitamins or minerals or by enhancing the expression of already present genes that are involved in nutrient biosynthesis (see Table 7). Transgenic techniques can be used to simultaneously incorporate genes that increase the concentration of micronutrients, their bioavailability and inhibit antinutritional factors (ANFs) in crops that restrict the utilization of nutrients (117). Transgenic approach presents a rational solution to improve the concentration and bioavailability of micronutrients (106, 118) especially when there is a limited genetic base present in different plant varieties (119, 120). Scientists have used biotechnology to develop crops that are high in beta-carotene, a precursor of Vitamin A, iron and zinc which are essential for human health but often lacking in the diet of people in developing countries. One of the examples is the development of vitamin A-rich rice called “golden rice,” which could help to address vitamin A deficiency in developing countries. Additionally, biotechnology can be used to improve the quality of food by increasing its shelf life, enhancing its flavor, reducing its allergenicity and producing food ingredients with health benefits like functional proteins, fibers and lipids. These ingredients can be used to improve the nutritional value of food products, making them more healthful and beneficial for consumers. This can help to make food more accessible and affordable for consumers, particularly in areas where food is scarce or expensive. It is a promising approach to improve the nutritional value of crops, but it is also a controversial issue and further research is needed to fully understand its potential impacts and risks. Vegetables and fruits The consumption of vegetables and fruits is important for a healthy diet, as they are rich in vitamins, minerals, fiber, and other essential nutrients. They offer a diverse, low-calorie, protective and nutrientdense diet. It has long been understood that eating the recommended amounts of vegetables and fruits has favorable health effects and frequent consumption of a range of these foods has been associated with decreased risk of diseases. The benefits of biofortifying vegetables and fruits include reducing the risk of chronic diseases, increasing economic productivity and promoting overall health and well-being. Biofortification through transgenic/ biotechnological means Biotechnology is a field of science that uses living organisms, cells or their components to make useful products or services. It has the potential to solve many of the world’s most pressing problems, such as producing enough food to feed a growing population, developing new Frontiers in Nutrition USDA Agricultural Research Service 10 frontiersin.org Avnee et al. 10.3389/fnut.2023.1233070 TABLE 7 Genes involved in micronutrient enrichment. Crops Wheat Genes involved Micronutrient References TaVIT1& TaVIT2 Fe and Mn (121) NAM B1, GPC 1 and PhyA Zn and Fe (122) Ama1 Tyrosine, lysin, (123) cysteine, methionine Rice CrtI, CrtB, Bacterial PSY Vitamin A Ferritin TaFer Fe (125, 126) (124) OsVIT1, OsVIT2, Fe (127) OsNAS1, OsNAS2 and OsNAS3 Fe and Zn (128, 129) AtTC, AtHP Vitamin E (130) THIC, THI1, TH1 Vitamin B1 (131) AtPDX1.1, AtPDX02 Vitamin B6 (132) ADCS, AtGTP cyclohydrolase 1 Vitamin B9 (133) Carotene desaturase, daffodil PSY Provitamin A (134) GmFAD3, ZmC1, chalcone synthase, phenylalanine ammonia Flavonoid, linoleic acid (135) Lyase Maize GmFER, aspergillus phytase, aspergillus phy2 Fe (117) Zmpsy 1 Vitamin A (136) crtI, crtB Vitamin A (124) HGGT Vitamin E, tocotrienol (136) Corynebacterium glutamicum cordapA Lysine (137) Sorghum LPA-1, PMI, PSY-1, CRT-I Vitamin A (138) Barley DHPS Lysine, Zn (117, 139) Phytase, AtZIP Fe (122, 139) Zn transporter gene Zn (117) AtVTE3, AtVTE4 Tocopherol (140) HvCs1F β-glucans (140) VLCPUFA Cholesterol-lowering agents PSY, crtB Carotenoids (142) Canola PSY, crtB, phytoene desaturase, and lycopene cyclase Carotenoids (142, 143) Aspartokinase (AK) and dihydrodipicolinic acid synthase (DHDPS) Lysine (144) Mustard FAD3 Linoleic acid (145) Soybean Phytoene synthase crtB β-carotene FATB1-A and FAD2-1A Linoleic acid SINCED1 Vitamin A, pectin and lycopene (147, 148) Delila, Rosea1, SIANT1 Anthocyanin (149, 150) Oilseeds Linseed Newton (141) (126, 146) (137) Vegetables Tomato Potato Cauliflower HMT, S3H, SAMT Iodine (151) GBSS Starch quality (152) FPGS, HPPK/DHPS Folate (153) AmA1, tar-1, Boxla, BoxIIa & BoxaIIa-2 Protein nptII Amylopectin component of starch Or gene β-carotene (3) (154) (155, 156) (Continued) Frontiers in Nutrition 11 frontiersin.org Avnee et al. 10.3389/fnut.2023.1233070 TABLE 7 (Continued) Crops Genes involved Micronutrient Cassava Erwinia crtB phytoene-synthase gene, & Arbidoopsis 1-deoxyxylulose-5- β-carotene References (157, 158) phosphate synthase Sweet potato EFA1 gene Fe (159) Ferritin FEA1 Fe (157) ASP 1, Zeolin Protein IbMYB1, npt II Anthocyanin, carotenoids and (160, 161) antioxidants Crtl, CrtB, CrtY, LCYe ß-Carotene Apple Stilbene synthase Antioxidants Banana PSY2a β-carotene (126, 162, 163) Fruits Challenges Waltz (165) an increasingly important area of study as the global population continues to hike and the demand for nutrient-rich food is growing. Scientists are working on developing new varieties of crops that are high in essential vitamins and minerals, such as iron, zinc and vitamin A. As research in this field continues to advance, it is likely that we will see an increasing number of nutrient-rich crop varieties that can help to address global malnutrition and improve public health. Improved plant uptake and absorption of crucial nutrients is the subject of another field of biofortification research. This includes the use of fertilizers and other agricultural practices that can increase the availability of nutrients in the soil and enhance the plants’ ability to absorb them. Overall, the future prospects for biofortification are very promising. Some potential benefits of biofortification include: Expanding the range of biofortified crops: Currently, the main focus of biofortification has been on staple crops such as rice, wheat and maize, but there is potential to biofortify other crops as well, such as fruits, vegetables and legumes. Improving the efficiency of biofortification: Scientists are working on ways to increase the nutrient content of crops using fewer resources, in order to make biofortification more cost-effective and sustainable. Improving the distribution and access to biofortified crops: This may involve developing new storage and transport technologies, as well as working with governments and other organizations to create supportive policies and infrastructure. Promoting the awareness and understanding of biofortification: This could involve educating the public about the benefits of biofortified crops and addressing any concerns or misconceptions about their safety or nutritional value. Reducing malnutrition and improving public health: Biofortification can increase the nutrient value of locally-grown crops, which can help to address deficiencies in essential vitamins and minerals and improve the nutritional status of populations that rely on these crops as a major source of energy and nutrients and contribute to food security by increasing the availability of nutritious foods. Supporting sustainable development: Biofortification can be implemented at a relatively low cost and can be integrated into existing farming practices, making it a sustainable and scalable solution for improving nutrition. There are several challenges that need to be overcome in order to effectively implement biofortification programs: Limited availability of biofortified varieties: Many biofortified crops are still in the development or testing phase and may not yet be widely available for cultivation. Limited awareness and understanding of biofortification: Many people may not be aware of the benefits of biofortified crops or may have misconceptions about their safety or nutritional value. Limited distribution and access: Even if biofortified crops are available, they may not reach the people who need them most, due to factors such as inadequate infrastructure, lack of storage facilities, or high costs. Political and regulatory challenges: The development and distribution of biofortified crops can be hindered by political and regulatory barriers, such as concerns about intellectual property rights, biosafety and trade issues. The development and commercialization of genetically modified (GM) crops, which are a potential tool for biofortification, can be subject to complex and often controversial regulations. Agricultural constraints: Biofortified crops may not always perform as well as non-biofortified varieties under certain growing conditions, such as drought or pests. Limited adoption: Even if biofortified crops are available, farmers may not choose to grow them if they are not familiar with the benefits or if they are not convinced that the crops will be more profitable. Consumer acceptance: Biofortified crops may be perceived as being different or inferior to non-biofortified varieties, which could affect consumer acceptance. Funding: Biofortification programs require ongoing funding in order to support research, development, and implementation efforts. Coordination: Biofortification programs often involve multiple stakeholders, including governments, NGOs, farmers, and the private sector. Ensuring effective coordination among these stakeholders can be challenging. Future prospects The discipline of biofortification has a number of intriguing research areas that hold great potential for the future. It is becoming Frontiers in Nutrition (164) 12 frontiersin.org Avnee et al. 10.3389/fnut.2023.1233070 Author contributions Improving gender equity: Women and children are often the most vulnerable to malnutrition, and biofortification can help to reduce gender disparities in access to nutritious foods. First draft designed by Avnee, reviewed by SS, DC and reviewed and revised by PJ and RR. All authors contributed to the article and approved the submitted version. Conclusion Acknowledgments Biofortification heralds a transformative paradigm in a battle against malnutrition and hidden hunger, which is often not visible to the naked eye, as people may appear well-nourished but still be deficient in essential vitamins and minerals. In some cases, biofortified crops may also have higher yields, which can help to improve food security and increase income for farmers. It can be a cost-effective and sustainable way to improve nutrition, as it relies on using existing agricultural infrastructure and practices. It can help to address dietary deficiencies and improve nutrition in low-income populations, which may not have the same access to nutrient-rich foods as those in higher-income groups. The integration of multinutrient biofortification and cutting-edge nano-technology marks a groundbreaking leap. However, there are several challenges that need to be overcome in order to effectively implement biofortification programs, including limited availability of biofortified varieties, high costs of production, limited awareness and understanding, limited distribution and access, and political and regulatory barriers. Biofortified food crops have the potential to significantly improve the lives and health of millions of underprivileged people in India with careful planning, execution, and implementation while requiring a low investment in research. 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Biofortification: an approach to eradicate micronutrient deficiency

Biofortification: an approach to eradicate micronutrient deficiency

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