Papers by Massimo Bellanda
Chemistry & Biodiversity, 2007
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Protein Science, 2006
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Journal of the American Chemical Society, 2000
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Chemistry - A European Journal, 2007
... Dr. 1 ,; Silvano Geremia Prof. Dr. 2 ,; Nicola Demitri 2 ,; Lucio Randaccio Prof. Dr. 2 ,; Qu... more ... Dr. 1 ,; Silvano Geremia Prof. Dr. 2 ,; Nicola Demitri 2 ,; Lucio Randaccio Prof. Dr. 2 ,; Quirinus B. Broxterman Dr. 3 ,; Bernard Kaptein Dr. 3 ,; Paolo Pengo Dr. 1 ,; Lucia Pasquato Prof. Dr. 2 ,; Paolo Scrimin Prof. ... Copyright © 1999–2012 John Wiley & Sons, Inc. All Rights Reserved.
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Chemistry - A European Journal, 2007
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Chemistry & Biodiversity, 2007
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Biochemistry, 2005
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Atherosclerosis Supplements, 2003
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Biopolymers, 2009
Cortactin is a ubiquitous actin-binding protein that regulates various aspects of cell dynamics a... more Cortactin is a ubiquitous actin-binding protein that regulates various aspects of cell dynamics and is implicated in the pathogenesis of human neoplasia. The sequence of cortactin contains a number of signaling motifs and an SH3 domain at the C-terminus, which mediates the interaction of the protein with several partners, including Shank2. A recombinant protein, comprising the murine cortactin SH3 domain fused to GST (GST-SH3(m-cort)), was prepared and used to assess the domain-binding affinity of potential peptide-ligands reproducing the proline-rich regions of human HPK1 and Shank2 proteins. The key residues involved in the SH3(m-cort) domain recognition were identified by three different approaches: non-immobilized ligand interaction assay by circular dichroism, isothermal titration calorimetry, and nuclear magnetic resonance. Our results show that the classical PxxPxK class II binding motif is not sufficient to mediate the interaction with GST-SH3(m-cort), an event that depends on the presence of additional basic residues located at either the N- or the C-terminus of the PxxPxK motif. Especially effective in promoting the peptide binding is a Lys residue at the -5 position, a determinant present in both P2 (HPK1 394-403) and S1 (Shank2 1168-1189) peptides. GST-SH3(m-cort) exhibits the highest affinity toward peptide S1, which contains additional Lys residues at the -3, -5, and -7 positions, indicating that the optimal consensus motif may be KPPxPxKxKxK. These results are supported by the in silico models of SH3(m-cort) complexed with P2 or S1, which highlight the domain residues that interact with the recognition determinants of the peptide-ligand and cooperate in binding stabilization.
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Biopolymers, 2006
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Disturbances in fatty acid metabolism are involved in the etiology of insulin resistance and the ... more Disturbances in fatty acid metabolism are involved in the etiology of insulin resistance and the related dyslipidemia, hypertension, and procoagulant state. The fatty acid trans- port proteins (FATPs) are implicated in facilitated cellular uptake of nonesterified fatty acids (NEFAs), thus potentially regulating NEFA concentrations and metabolism. The aim of this study was to investigate polymorphic loci in the FATP4 gene
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Molecular Dynamics On Parallel Computers, 2000
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Angewandte Chemie (International ed. in English), Jan 14, 2004
... Massimo Bellanda Dr. 1 ,; Mario Rainaldi Dr. 1 ,; Quirinus B. Broxterman Dr. 2 ,; Bernard Kap... more ... Massimo Bellanda Dr. 1 ,; Mario Rainaldi Dr. 1 ,; Quirinus B. Broxterman Dr. 2 ,; Bernard Kaptein Dr. 2 ,; Fernando Formaggio Prof. 1 ,; Marco Crisma Dr. 1 ,; Stefano Mammi Prof. 1 ,; Claudio Toniolo Prof. 1. ... C. Mazzuca, F. Formaggio, C. Toniolo, QB Broxterman, J. Phys. Chem. ...
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Journal of Biomolecular Nmr, 2002
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Protein Science, 2006
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Journal of the American Chemical Society, 2000
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Journal of Molecular Biology, 2010
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Chemistry - A European Journal, 2007
... Dr. 1 ,; Silvano Geremia Prof. Dr. 2 ,; Nicola Demitri 2 ,; Lucio Randaccio Prof. Dr. 2 ,; Qu... more ... Dr. 1 ,; Silvano Geremia Prof. Dr. 2 ,; Nicola Demitri 2 ,; Lucio Randaccio Prof. Dr. 2 ,; Quirinus B. Broxterman Dr. 3 ,; Bernard Kaptein Dr. 3 ,; Paolo Pengo Dr. 1 ,; Lucia Pasquato Prof. Dr. 2 ,; Paolo Scrimin Prof. ... Copyright © 1999–2012 John Wiley & Sons, Inc. All Rights Reserved.
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Chemistry - A European Journal, 2007
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Chemistry & Biodiversity, 2007
The total synthesis in solution and chemical characterization of the antibacterial undecamer pept... more The total synthesis in solution and chemical characterization of the antibacterial undecamer peptaibol cervinin, its C-terminal (Lol-Ac) derivative, also naturally occurring, and three selected analogues modified at the N- and/or C-terminus(i) to improve the affinity to the membrane environment, are described. A solution conformational analysis in different media, performed by the combined use of FT-IR, CD, and 2D-NMR techniques, clearly shows a significant 3(10)-like helicity for these Aib-Pro rich peptides. The hydrophilicity/lipophilicity characteristics of the final compounds significantly influence their membrane-modifying properties.
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Papers by Massimo Bellanda