Tensional homeostasis in dermal fibroblasts: mechanical responses to mechanical loading in three-dimensional substrates.

Brown RA ¹,

Prajapati R ,

McGrouther DA ,

Yannas IV ,

Eastwood M

Affiliations

1. Division of Plastic Surgery, Rayne Institute, University College London, United Kingdom.
Authors
Brown RA¹
(1 author)

ORCIDs linked to this article

Yannas IV | 0000-0003-0151-708X

Journal of Cellular Physiology, 01 Jun 1998, 175(3):323-332
3.0.co;2-6">https://doi.org/10.1002/(sici)1097-4652(199806)175:3<323::aid-jcp10>3.0.co;2-6 PMID: 9572477

Abstract

Many soft connective tissues are under endogenous tension, and their resident cells generate considerable contractile forces on the extracellular matrix. The present work was aimed to determine quantitatively how fibroblasts, grown within three-dimensional collagen lattices, respond mechanically to precisely defined tensional loads. Forces generated in response to changes in applied load were measured using a tensional culture force monitor. In a number of variant systems, resident cells consistently reacted to modify the endogenous matrix tension in the opposite direction to externally applied loads. That is, increased external loading was followed immediately by a reduction in cell-mediated contraction whilst decreased external loading elicited increased contraction. Responses were cell-mediated and not a result of material properties of the matrices. This is the first detailed characterisation of a tensional homeostatic response in cells. The maintained force, after 8 h in culture, was typically around 40-60 dynes/million cells). Maintenance of an active tensional homeostasis has widespread implications for cells in culture and for whole tissue function.

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References

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Tensional homeostasis in dermal fibroblasts: mechanical responses to mechanical loading in three-dimensional substrates.

Affiliations

ORCIDs linked to this article

Abstract

Full text links

References

Title not supplied

Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro.

Axonal growth in response to experimentally applied mechanical tension.

Balanced mechanical forces and microtubule contribution to fibroblast contraction.

Effect of compressive loading and unloading on the synthesis of total protein, proteoglycan, and fibronectin by canine cartilage explants.

Mechanical load and polypeptide growth factors stimulate cardiac fibroblast activity.

The effect of proteolytic degradation of plasma fibronectin on the responses of functional and immunometric assays for intact fibronectin.

Stretch-induced parathyroid hormone-related peptide gene expression in the rat uterus.

Measurement of mechanical forces generated by skin fibroblasts embedded in a three-dimensional collagen gel.

Tension and compression in the cytoskeleton of PC-12 neurites. II: Quantitative measurements.

Citations & impact

Impact metrics

Citations of article over time

Alternative metrics

Article citations

Focal adhesion kinase regulates tendon cell mechanoresponse and physiological tendon development.

Fibroblast and myofibroblast activation in normal tissue repair and fibrosis.

Cancer-associated Fibroblast-specific Expression of the Matricellular Protein CCN1 Coordinates Neovascularization and Stroma Deposition in Melanoma Metastasis.

A two-scale numerical study on the mechanobiology of abdominal aortic aneurysms.

Implications of Cellular Mechanical Memory in Bioengineering.

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Tensional homeostasis in dermal fibroblasts: mechanical responses to mechanical loading in three-dimensional substrates.

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