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An Escherichia coli host strain useful for efficient overproduction of cloned gene products with NaCl as the inducer.

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Affiliations

  • 1. Centre for Cellular and Molecular Biology, Hyderabad, India.
      Authors
    • Bhandari P 1
    (1 author)

Journal of Bacteriology, 01 Jul 1997, 179(13):4403-4406
https://doi.org/10.1128/jb.179.13.4403-4406.1997 PMID: 9209061 PMCID: PMC179267

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Abstract 

Salt-induced overexpression of genes cloned downstream of the phage T7 phi10 promoter was demonstrated in an Escherichia coli strain (GJ1158) which carries a single chromosomally integrated copy of the gene for phage T7 RNA polymerase under transcriptional control of the cis-regulatory elements of the osmoresponsive proU operon. Plasmids that have been constructed to obtain overproduction of individual target gene products in strain BL21(DE3) (by addition of isopropyl-beta-D-thiogalactopyranoside as an inducer) can directly be transformed into GJ1158. The NaCl induction regimen was also shown to be associated with a decreased propensity for sequestration of overexpressed target proteins within insoluble inclusion bodies.

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J Bacteriol. 1997 Jul; 179(13): 4403–4406.
PMCID: PMC179267
PMID: 9209061

An Escherichia coli host strain useful for efficient overproduction of cloned gene products with NaCl as the inducer.

P Bhandari and J Gowrishankar
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Abstract

Salt-induced overexpression of genes cloned downstream of the phage T7 phi10 promoter was demonstrated in an Escherichia coli strain (GJ1158) which carries a single chromosomally integrated copy of the gene for phage T7 RNA polymerase under transcriptional control of the cis-regulatory elements of the osmoresponsive proU operon. Plasmids that have been constructed to obtain overproduction of individual target gene products in strain BL21(DE3) (by addition of isopropyl-beta-D-thiogalactopyranoside as an inducer) can directly be transformed into GJ1158. The NaCl induction regimen was also shown to be associated with a decreased propensity for sequestration of overexpressed target proteins within insoluble inclusion bodies.

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Selected References
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