Europe PMC
Nothing Special   »   [go: up one dir, main page]

Europe PMC requires Javascript to function effectively.

Either your web browser doesn't support Javascript or it is currently turned off. In the latter case, please turn on Javascript support in your web browser and reload this page.

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Cooperation of kinetochores and pole in the establishment of monopolar mitotic apparatus.

Proceedings of the National Academy of Sciences of the United States of America, 01 Jan 1981, 78(1):377-381
https://doi.org/10.1073/pnas.78.1.377 PMID: 6941253 PMCID: PMC319056

Free full text in Europe PMC

Abstract 

Monopolar mitotic apparatus can be produced in sea urchin eggs by a manoeuvre that distributes the four poles of the second mitosis into four separate blastomeres. The pole of the monopolar mitotic apparatus generates a half-spindle that is similar in structural details to the half-spindle of a normal bipolar mitotic apparatus, although the chromosomes are not as well aligned as in a normal metaphase plate. The chromosomes are oriented; one kinetochore faces the pole while its sister kinetochore faces away from the pole. The poleward kinetochore is connected to the pole by bundles of microtubules. No microtubules are seen on the sister kinetochore that faces away from the pole. Therefore, a single pole can direct most of the events in the establishment of a mitotic apparatus. Our interpretation examines the cooperation of kinetochores and poles in the formation of microtubules between them, stressing the half-spindle as the medium of cooperation and leaving open the question whether the kinetochores are origins or terminations of microtubules.

Free full text 

Logo of pnasLink to Publisher's site
Proc Natl Acad Sci U S A. 1981 Jan; 78(1): 377–381.
PMCID: PMC319056
PMID: 6941253

Cooperation of kinetochores and pole in the establishment of monopolar mitotic apparatus.

D Mazia, N Paweletz, G Sluder, and E M Finze
Copyright and License information Disclaimer

Abstract

Monopolar mitotic apparatus can be produced in sea urchin eggs by a manoeuvre that distributes the four poles of the second mitosis into four separate blastomeres. The pole of the monopolar mitotic apparatus generates a half-spindle that is similar in structural details to the half-spindle of a normal bipolar mitotic apparatus, although the chromosomes are not as well aligned as in a normal metaphase plate. The chromosomes are oriented; one kinetochore faces the pole while its sister kinetochore faces away from the pole. The poleward kinetochore is connected to the pole by bundles of microtubules. No microtubules are seen on the sister kinetochore that faces away from the pole. Therefore, a single pole can direct most of the events in the establishment of a mitotic apparatus. Our interpretation examines the cooperation of kinetochores and poles in the formation of microtubules between them, stressing the half-spindle as the medium of cooperation and leaving open the question whether the kinetochores are origins or terminations of microtubules.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (3.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
 
icon of scanned page 377
377
icon of scanned page 378
378
icon of scanned page 379
379
icon of scanned page 380
380
icon of scanned page 381
381
 

Images in this article

Image
Image
on p.378
Image
Image
on p.379
Image
Image
on p.380
Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • HARRIS P. Some structural and functional aspects of the mitotic apparatus in sea urchin embryos. J Cell Biol. 1962 Sep;14:475–487. [Europe PMC free article] [Abstract] [Google Scholar]
  • Paweletz N, Mazia D. Fine structure of the mitotic cycle of unfertilized sea urchin eggs activated by ammoniacal sea water. Eur J Cell Biol. 1979 Oct;20(1):37–44. [Abstract] [Google Scholar]
  • Bajer A. Chromosome movement and fine structure of the mitotic spindle. Symp Soc Exp Biol. 1968;22:285–310. [Abstract] [Google Scholar]
  • Telzer BR, Moses MJ, Rosenbaum JL. Assembly of microtubules onto kinetochores of isolated mitotic chromosomes of HeLa cells. Proc Natl Acad Sci U S A. 1975 Oct;72(10):4023–4027. [Europe PMC free article] [Abstract] [Google Scholar]
  • Tippit DH, Pickett-Heaps JD, Leslie R. Cell division in two large pennate diatoms Hantzschia and Nitzschia III. A new proposal for kinetochore function during prometaphase. J Cell Biol. 1980 Aug;86(2):402–416. [Europe PMC free article] [Abstract] [Google Scholar]
  • Andersen B, Osborn M, Weber K. Specific visualization of the distribution of the calcium dependent regulatory protein of cyclic nucleotide phosphodiesterase (modulator protein) in tissue culture cells by immunofluorescence microscopy: mitosis and intercellular bridge. Cytobiologie. 1978 Aug;17(2):354–364. [Abstract] [Google Scholar]
  • Welsh MJ, Dedman JR, Brinkley BR, Means AR. Calcium-dependent regulator protein: localization in mitotic apparatus of eukaryotic cells. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1867–1871. [Europe PMC free article] [Abstract] [Google Scholar]
Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Full text links 

Read article at publisher's site: https://doi.org/10.1073/pnas.78.1.377

Read article for free, from open access legal sources, via Unpaywall: https://europepmc.org/articles/pmc319056?pdf=renderUnpaywall

Citations & impact 

Impact metrics

28
Citations
Jump to Citations

Citations of article over time

Article citations

Go to all (28) article citations

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.

Funding 

Funders who supported this work.

NIGMS NIH HHS (1)