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A Single-Cell Atlas of the Human Healthy Airways.

Author information

Affiliations

  • 1. Université Côte d'Azur, CNRS, Institut Pharmacologie Moléculaire et Cellulaire, Sophia-Antipolis, France.
      Authors
    • Deprez M 1
    • Zaragosi LE 1
    • Truchi M 1
    • Becavin C 1
    • Ruiz García S 1
    • Arguel MJ 1
    • Plaisant M 1
    • Magnone V 1
    • Lebrigand K 1
    • Abelanet S 1
    • Brau F 1
    • Paquet A 1
    • Leroy S 1, 3
    • Barbry P 1
    (14 authors)
  • 2. Memorial Sloan Kettering Cancer Center, New York, New York; and.
      Authors
    • Pe'er D 2
    (1 author)
  • 3. Université Côte d'Azur, Centre Hospitalier Universitaire de Nice, Fédération Hospitalo-Universitaire OncoAge, CNRS, Inserm, Institute for Research on Cancer and Aging Nice Team 3, Pulmonology Department, Nice, France.
      Authors
    • Marquette CH 3
    • Leroy S 1, 3
    (2 authors)

ORCIDs linked to this article

Show all (6)

American Journal of Respiratory and Critical Care Medicine, 01 Dec 2020, 202(12):1636-1645
https://doi.org/10.1164/rccm.201911-2199oc PMID: 32726565 

A comment on this article appears in "Toward a Cell Atlas of the Human Airway." Am J Respir Crit Care Med. 2020 Dec 15;202(12):1611-1612. This article is based on a previously available preprint.

Abstract 

Rationale: The respiratory tract constitutes an elaborate line of defense that is based on a unique cellular ecosystem.Objectives: We aimed to investigate cell population distributions and transcriptional changes along the airways by using single-cell RNA profiling.Methods: We have explored the cellular heterogeneity of the human airway epithelium in 10 healthy living volunteers by single-cell RNA profiling. A total of 77,969 cells were collected at 35 distinct locations, from the nose to the 12th division of the airway tree.Measurements and Main Results: The resulting atlas is composed of a high percentage of epithelial cells (89.1%) but also immune (6.2%) and stromal (4.7%) cells with distinct cellular proportions in different regions of the airways. It reveals differential gene expression between identical cell types (suprabasal, secretory, and multiciliated cells) from the nose (MUC4, PI3, SIX3) and tracheobronchial (SCGB1A1, TFF3) airways. By contrast, cell-type-specific gene expression is stable across all tracheobronchial samples. Our atlas improves the description of ionocytes, pulmonary neuroendocrine cells, and brush cells and identifies a related population of NREP-positive cells. We also report the association of KRT13 with dividing cells that are reminiscent of previously described mouse "hillock" cells and with squamous cells expressing SCEL and SPRR1A/B.Conclusions: Robust characterization of a single-cell cohort in healthy airways establishes a valuable resource for future investigations. The precise description of the continuum existing from the nasal epithelium to successive divisions of the airways and the stable gene expression profile of these regions better defines conditions under which relevant tracheobronchial proxies of human respiratory diseases can be developed.

Full text links 

Read article at publisher's site: https://doi.org/10.1164/rccm.201911-2199oc

Read article for free, from open access legal sources, via Unpaywall: https://www.biorxiv.org/content/biorxiv/early/2019/12/23/2019.12.21.884759.full.pdfUnpaywall

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