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Localization of topoisomerase II in mitotic chromosomes.

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The Journal of Cell Biology, 01 May 1985, 100(5):1716-1725
https://doi.org/10.1083/jcb.100.5.1716 PMID: 2985626 PMCID: PMC2113864

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Abstract 

In the preceding article we described a polyclonal antibody that recognizes cSc-1, a major polypeptide component of the chicken mitotic chromosome scaffold. This polypeptide was shown to be chicken topoisomerase II. In the experiments described in the present article we use indirect immunofluorescence and immunoelectron microscopy to examine the distribution of topoisomerase II within intact chromosomes. We also describe a simple experimental protocol that differentiates antigens that are interspersed along the chromatin fiber from those that occupy restricted domains within the chromosome. These experiments indicate that the distribution of the enzyme appears to be independent of the bulk chromatin. Our data suggest that topoisomerase II is bound to the bases of the radial loop domains of mitotic chromosomes.

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J Cell Biol. 1985 May 1; 100(5): 1716–1725.
PMCID: PMC2113864
PMID: 2985626

Localization of topoisomerase II in mitotic chromosomes

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Abstract

In the preceding article we described a polyclonal antibody that recognizes cSc-1, a major polypeptide component of the chicken mitotic chromosome scaffold. This polypeptide was shown to be chicken topoisomerase II. In the experiments described in the present article we use indirect immunofluorescence and immunoelectron microscopy to examine the distribution of topoisomerase II within intact chromosomes. We also describe a simple experimental protocol that differentiates antigens that are interspersed along the chromatin fiber from those that occupy restricted domains within the chromosome. These experiments indicate that the distribution of the enzyme appears to be independent of the bulk chromatin. Our data suggest that topoisomerase II is bound to the bases of the radial loop domains of mitotic chromosomes.

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Selected References

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  • Miller OL., Jr Fine structure of lampbrush chromosomes. Natl Cancer Inst Monogr. 1965 Dec;18:79–99. [Abstract] [Google Scholar]
  • Rattner JB, Goldsmith M, Hamkalo BA. Chromatin organization during meiotic prophase of Bombyx mori. Chromosoma. 1980;79(2):215–224. [Abstract] [Google Scholar]
  • Rattner JB, Goldsmith MR, Hamkalo BA. Chromosome organization during male meiosis in Bombyx mori. Chromosoma. 1981;82(3):341–351. [Abstract] [Google Scholar]
  • Bahr GF. Human chromosome fibers. Considerations of DNA-protein packing and of looping patterns. Exp Cell Res. 1970 Sep;62(1):39–49. [Abstract] [Google Scholar]
  • Paulson JR, Laemmli UK. The structure of histone-depleted metaphase chromosomes. Cell. 1977 Nov;12(3):817–828. [Abstract] [Google Scholar]
  • Marsden MP, Laemmli UK. Metaphase chromosome structure: evidence for a radial loop model. Cell. 1979 Aug;17(4):849–858. [Abstract] [Google Scholar]
  • Earnshaw WC, Laemmli UK. Architecture of metaphase chromosomes and chromosome scaffolds. J Cell Biol. 1983 Jan;96(1):84–93. [Europe PMC free article] [Abstract] [Google Scholar]
  • Laemmli UK, Cheng SM, Adolph KW, Paulson JR, Brown JA, Baumbach WR. Metaphase chromosome structure: the role of nonhistone proteins. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 1):351–360. [Abstract] [Google Scholar]
  • Rattner JB, Branch A, Hamkalo BA. Electron microscopy of whole mount metaphase chromosomes. Chromosoma. 1975 Nov 11;52(4):329–338. [Abstract] [Google Scholar]
  • Okada TA, Comings DE. A search for protein cores in chromosomes: is the scaffold an artifact? Am J Hum Genet. 1980 Nov;32(6):814–832. [Europe PMC free article] [Abstract] [Google Scholar]
  • Labhart P, Koller T, Wunderli H. Involvement of higher order chromatin structures in metaphase chromosome organization. Cell. 1982 Aug;30(1):115–121. [Abstract] [Google Scholar]
  • Howell WM, Hsu TC. Chromosome core structure revealed by silver staining. Chromosoma. 1979 Jun 21;73(1):61–66. [Abstract] [Google Scholar]
  • Pathak S, Hsu TC. Silver-stained structures in mammalian meiotic prophase. Chromosoma. 1979 Jan 8;70(2):195–203. [Abstract] [Google Scholar]
  • Fletcher JM. Light microscope analysis of meiotic prophase chromosomes by silver staining. Chromosoma. 1979 Apr 30;72(2):241–248. [Abstract] [Google Scholar]
  • Dresser ME, Moses MJ. Silver staining of synaptonemal complexes in surface spreads for light and electron microscopy. Exp Cell Res. 1979 Jul;121(2):416–419. [Abstract] [Google Scholar]
  • Earnshaw WC, Laemmli UK. Silver staining the chromosome scaffold. Chromosoma. 1984;89(3):186–192. [Abstract] [Google Scholar]
  • Earnshaw WC, Halligan B, Cooke CA, Heck MM, Liu LF. Topoisomerase II is a structural component of mitotic chromosome scaffolds. J Cell Biol. 1985 May;100(5):1706–1715. [Europe PMC free article] [Abstract] [Google Scholar]
  • Labhart P, Koller T. Electron microscope specimen preparation of rat liver chromatin by a modified Miller spreading technique. Eur J Cell Biol. 1981 Jun;24(2):309–316. [Abstract] [Google Scholar]
  • Geoghegan WD, Ackerman GA. Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application. J Histochem Cytochem. 1977 Nov;25(11):1187–1200. [Abstract] [Google Scholar]
  • Horisberger M, Rosset J. Colloidal gold, a useful marker for transmission and scanning electron microscopy. J Histochem Cytochem. 1977 Apr;25(4):295–305. [Abstract] [Google Scholar]
  • Williamson DH, Fennell DJ. The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA. Methods Cell Biol. 1975;12:335–351. [Abstract] [Google Scholar]
  • Takagi N, Sasaki M. A phylogenetic study of bird karyotypes. Chromosoma. 1974 May 21;46(1):91–120. [Abstract] [Google Scholar]
  • Halligan BD, Edwards KA, Liu LF. Purification and characterization of a type II DNA topoisomerase from bovine calf thymus. J Biol Chem. 1985 Feb 25;260(4):2475–2482. [Abstract] [Google Scholar]
  • Bak AL, Zeuthen J, Crick FH. Higher-order structure of human mitotic chromosomes. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1595–1599. [Europe PMC free article] [Abstract] [Google Scholar]
  • Sedat J, Manuelidis L. A direct approach to the structure of eukaryotic chromosomes. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 1):331–350. [Abstract] [Google Scholar]
  • Okada TA, Comings DE. Higher order structure of chromosomes. Chromosoma. 1979 Apr 5;72(1):1–14. [Abstract] [Google Scholar]
  • Saragosti S, Moyne G, Yaniv M. Absence of nucleosomes in a fraction of SV40 chromatin between the origin of replication and the region coding for the late leader RNA. Cell. 1980 May;20(1):65–73. [Abstract] [Google Scholar]
  • Chen GL, Yang L, Rowe TC, Halligan BD, Tewey KM, Liu LF. Nonintercalative antitumor drugs interfere with the breakage-reunion reaction of mammalian DNA topoisomerase II. J Biol Chem. 1984 Nov 10;259(21):13560–13566. [Abstract] [Google Scholar]
  • Adolph KW, Cheng SM, Laemmli UK. Role of nonhistone proteins in metaphase chromosome structure. Cell. 1977 Nov;12(3):805–816. [Abstract] [Google Scholar]
  • Liu LF. DNA topoisomerases--enzymes that catalyse the breaking and rejoining of DNA. CRC Crit Rev Biochem. 1983;15(1):1–24. [Abstract] [Google Scholar]
  • Singh B, Gupta RS. Mutagenic responses of thirteen anticancer drugs on mutation induction at multiple genetic loci and on sister chromatid exchanges in Chinese hamster ovary cells. Cancer Res. 1983 Feb;43(2):577–584. [Abstract] [Google Scholar]
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