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Mitotic chromosome assembly despite nucleosome depletion in Xenopus egg extracts.

Author information

Affiliations

  • 1. Chromosome Dynamics Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.
      Authors
    • Shintomi K 1
    • Hirano T 1
    (2 authors)
  • 2. Department of Life Science, Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan.
      Authors
    • Inoue F 2
    • Watanabe H 2
    • Ohsugi M 2
    (3 authors)
  • 3. Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Aichi 464-8602, Japan.
      Authors
    • Ohsumi K 3
    (1 author)

ORCIDs linked to this article

Science, 18 May 2017, 356(6344):1284-1287
https://doi.org/10.1126/science.aam9702 PMID: 28522692 

A comment on this article appears in "Building chromosomes without bricks." Science. 2017 Jun 23;356(6344):1233-1234.

Abstract 

The nucleosome is the fundamental structural unit of eukaryotic chromatin. During mitosis, duplicated nucleosome fibers are organized into a pair of rod-shaped structures (chromatids) within a mitotic chromosome. However, it remains unclear whether nucleosome assembly is indeed an essential prerequisite for mitotic chromosome assembly. We combined mouse sperm nuclei and Xenopus cell-free egg extracts depleted of the histone chaperone Asf1 and found that chromatid-like structures could be assembled even in the near absence of nucleosomes. The resultant "nucleosome-depleted" chromatids contained discrete central axes positive for condensins, although they were more fragile than normal nucleosome-containing chromatids. Combinatorial depletion experiments underscored the central importance of condensins in mitotic chromosome assembly, which sheds light on their functional cross-talk with nucleosomes in this process.

Full text links 

Read article at publisher's site: https://doi.org/10.1126/science.aam9702

Read article for free, from open access legal sources, via Unpaywall: https://science.sciencemag.org/content/sci/356/6344/1284.full.pdfUnpaywall

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Funding 

Funders who supported this work.

Japan Society for the Promotion of Science (8)

Japan Society for the Promotion ofScience (2)