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The role of cyclins in the maturation of Patella vulgata oocytes.

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  • 1. Ecole Normale Superieure, Laboratoire de Biologie Cellulaire et Moleculaire, Lyon, France.
      Authors
    • van Loon AE 1
    (1 author)

ORCIDs linked to this article

The EMBO Journal, 01 Nov 1991, 10(11):3343-3349
https://doi.org/10.1002/j.1460-2075.1991.tb04898.x PMID: 1655419 PMCID: PMC453061

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Abstract 

We have cloned and sequenced the cDNAs encoding Patella vulgata cyclins A and B. The cDNA clones contain an open reading frame of 426 and 408 amino acids respectively, which present similarity with cyclins from other species. Cyclin A and B RNAs are present as polyadenylated and non-polyadenylated RNA in prophase oocytes and are completely polyadenylated in metaphase I. During the first cleavages after fertilization the level of cyclin A and B mRNAs is high and drops when the free swimming stage is reached. Using p13suc1-Sepharose bead precipitation we demonstrate that cyclin synthesis is triggered during maturation and that inhibition of protein synthesis makes the cyclins disappear rapidly from the metaphase I oocytes, which shift to interphase condition. By microinjecting antisense oligonucleotides into metaphase I oocytes, we demonstrate that in vivo ablation of cyclin A and B messengers together gives the same result, whereas microinjection of only one oligonucleotide does not show any effect.

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EMBO J. 1991 Nov; 10(11): 3343–3349.
PMCID: PMC453061
PMID: 1655419

The role of cyclins in the maturation of Patella vulgata oocytes.

A E van Loon, P Colas, H J Goedemans, I Néant, P Dalbon, and P Guerrier
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Abstract

We have cloned and sequenced the cDNAs encoding Patella vulgata cyclins A and B. The cDNA clones contain an open reading frame of 426 and 408 amino acids respectively, which present similarity with cyclins from other species. Cyclin A and B RNAs are present as polyadenylated and non-polyadenylated RNA in prophase oocytes and are completely polyadenylated in metaphase I. During the first cleavages after fertilization the level of cyclin A and B mRNAs is high and drops when the free swimming stage is reached. Using p13suc1-Sepharose bead precipitation we demonstrate that cyclin synthesis is triggered during maturation and that inhibition of protein synthesis makes the cyclins disappear rapidly from the metaphase I oocytes, which shift to interphase condition. By microinjecting antisense oligonucleotides into metaphase I oocytes, we demonstrate that in vivo ablation of cyclin A and B messengers together gives the same result, whereas microinjection of only one oligonucleotide does not show any effect.

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Selected References

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