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Increased apoptotic peripheral blood neutrophils in systemic lupus erythematosus: relations with disease activity, antibodies to double stranded DNA, and neutropenia.

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Affiliations

  • 1. Department of Rheumatology, Musgrave Park Hospital, Stockman's Lane, Belfast BT9 7JB.
      Authors
    • Courtney PA 1
    (1 author)

Annals of the Rheumatic Diseases, 01 May 1999, 58(5):309-314
https://doi.org/10.1136/ard.58.5.309 PMID: 10225817 PMCID: PMC1752888

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Abstract 

Objective

To quantify the percentage of apoptotic peripheral blood neutrophils in systemic lupus erythematosus (SLE) and to determine the relations with disease activity and neutropenia.

Methods

Neutrophil apoptosis in SLE patients (n =50) was assessed by flow cytometry using annexin V binding and fluorescent labelled anti-fas. Rheumatoid arthritis (RA, n =20) and inflammatory bowel disease patients (IBD, n =20) were studied as disease controls.

Results

The percentage of apoptotic neutrophils, determined by annexin V binding, was increased in peripheral blood of SLE patients (median = 3.25%) compared with normal healthy donors (n =20, median = 1.20%) and disease controls (RA: median = 1.15%) (IBD: median = 1.15%). SLE neutrophil apoptosis correlated positively with lupus disease activity measured by SLAM score. SLE patients with increased antibodies to dsDNA (>10 mg/ml) had increased apoptotic neutrophils. Eight of 14 neutropenic SLE patients had increased apoptotic neutrophils. Increased neutrophil fas expression compared with normal controls was observed in SLE, RA, and IBD.

Conclusion

Neutrophil fas expression is increased non-specifically in inflammatory disease. Increased circulating apoptotic neutrophils in SLE correlate positively with disease activity (SLAM) and may contribute to autoantigen excess including dsDNA.

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Logo of annrheumdLink to Publisher's site
Ann Rheum Dis. 1999 May; 58(5): 309–314.
PMCID: PMC1752888
PMID: 10225817

Increased apoptotic peripheral blood neutrophils in systemic lupus erythematosus: relations with disease activity, antibodies to double stranded DNA, and neutropenia

P Courtney, A Crockard, K Williamson, A Irvine, R Kennedy, and A Bell
Author information Copyright and License information Disclaimer

Abstract

OBJECTIVE—To quantify the percentage of apoptotic peripheral blood neutrophils in systemic lupus erythematosus (SLE) and to determine the relations with disease activity and neutropenia.
METHODS—Neutrophil apoptosis in SLE patients (n =50) was assessed by flow cytometry using annexin V binding and fluorescent labelled anti-fas. Rheumatoid arthritis (RA, n =20) and inflammatory bowel disease patients (IBD, n =20) were studied as disease controls.
RESULTS—The percentage of apoptotic neutrophils, determined by annexin V binding, was increased in peripheral blood of SLE patients (median = 3.25%) compared with normal healthy donors (n =20, median = 1.20%) and disease controls (RA: median = 1.15%) (IBD: median = 1.15%). SLE neutrophil apoptosis correlated positively with lupus disease activity measured by SLAM score. SLE patients with increased antibodies to dsDNA (>10 mg/ml) had increased apoptotic neutrophils. Eight of 14 neutropenic SLE patients had increased apoptotic neutrophils. Increased neutrophil fas expression compared with normal controls was observed in SLE, RA, and IBD.
CONCLUSION—Neutrophil fas expression is increased non-specifically in inflammatory disease. Increased circulating apoptotic neutrophils in SLE correlate positively with disease activity (SLAM) and may contribute to autoantigen excess including dsDNA.

Full Text

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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Figures and Tables

An external file that holds a picture, illustration, etc. Object name is 98290.f1.jpg

Flow cytometric histograms showing: (A) separated neutrophils from an SLE patient defined by forward angle (FS) and side scatter (SSC) light properties. (B) CD15 positivity (96%) (shaded area) of the gated neutrophil population and isotype control profile (white area). (C) Fas positivity (68%) (shaded area) of the gated neutrophil population and isotype control profile (white area). (D) FITC Annexin V and propidium iodide staining of the gated neutrophil population demonstrating 12% apoptotic cells (quadrant 4; FITC+, PI-).

An external file that holds a picture, illustration, etc. Object name is 98290.f2.jpg

Percentage annexin V positive neutrophils in normal controls, SLE patients, RA patients and IBD patients. Each point represents one patient, and the median for each group is shown.

An external file that holds a picture, illustration, etc. Object name is 98290.f3.jpg

Percentage neutrophil fas expression in normal controls, SLE patients, RA patients, and IBD patients. Each point represents an individual patient and the median for each group is shown.

An external file that holds a picture, illustration, etc. Object name is 98290.f4.jpg

Scatter plot demonstrating positive correlation between percentage annexin V positive neutrophils and SLAM (r =0.50, p=0.001). Each point represents an individual SLE patient.

An external file that holds a picture, illustration, etc. Object name is 98290.f5.jpg

Percentage annexin V expression in SLE patients stratified according to the concentration of antibodies to double stranded DNA (mg/ml). The 5-10 group includes concentrations greater than 5.0 and less than or equal to 10.0. Each point represents an individual patient and medians are marked for each group.

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

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