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The Drosophila ecdysone receptor (EcR) gene is required maternally for normal oogenesis.

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Affiliations

  • 1. Department of Genetics, University of Georgia, Athens, Georgia 30602, USA.
      Authors
    • Carney GE 1
    (1 author)

Genetics, 01 Mar 2000, 154(3):1203-1211
https://doi.org/10.1093/genetics/154.3.1203 PMID: 10757764 PMCID: PMC1461007

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Abstract 

Oogenesis in Drosophila is regulated by the steroid hormone ecdysone and the sesquiterpenoid juvenile hormone. Response to ecdysone is mediated by a heteromeric receptor composed of the EcR and USP proteins. We have identified a temperature-sensitive EcR mutation, EcR(A483T), from a previously isolated collection of EcR mutations. EcR(A483T) is predicted to affect all EcR protein products (EcR-A, EcR-B1, and EcR-B2) since it maps to a common exon encoding the ligand-binding domain. In wild-type females, we find that both EcR-A and EcR-B1 are expressed in nurse cells and follicle cells throughout oogenesis. EcR mutant females raised at permissive temperature and then shifted to restrictive temperature exhibit severe reductions in fecundity. Oogenesis in EcR mutant females is defective, and the spectrum of oogenic defects includes the presence of abnormal egg chambers and loss of vitellogenic egg stages. Our results demonstrate a requirement for EcR during female reproduction and suggest that EcR is required for normal oogenesis.

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Logo of geneticsLink to Publisher's site
Genetics. 2000 Mar; 154(3): 1203–1211.
PMCID: PMC1461007
PMID: 10757764

The Drosophila ecdysone receptor (EcR) gene is required maternally for normal oogenesis.

G E Carney and M Bender
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Abstract

Oogenesis in Drosophila is regulated by the steroid hormone ecdysone and the sesquiterpenoid juvenile hormone. Response to ecdysone is mediated by a heteromeric receptor composed of the EcR and USP proteins. We have identified a temperature-sensitive EcR mutation, EcR(A483T), from a previously isolated collection of EcR mutations. EcR(A483T) is predicted to affect all EcR protein products (EcR-A, EcR-B1, and EcR-B2) since it maps to a common exon encoding the ligand-binding domain. In wild-type females, we find that both EcR-A and EcR-B1 are expressed in nurse cells and follicle cells throughout oogenesis. EcR mutant females raised at permissive temperature and then shifted to restrictive temperature exhibit severe reductions in fecundity. Oogenesis in EcR mutant females is defective, and the spectrum of oogenic defects includes the presence of abnormal egg chambers and loss of vitellogenic egg stages. Our results demonstrate a requirement for EcR during female reproduction and suggest that EcR is required for normal oogenesis.

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Selected References

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  • Hawkins NC, Thorpe J, Schüpbach T. Encore, a gene required for the regulation of germ line mitosis and oocyte differentiation during Drosophila oogenesis. Development. 1996 Jan;122(1):281–290. [Abstract] [Google Scholar]
  • Henrich VC, Sliter TJ, Lubahn DB, MacIntyre A, Gilbert LI. A steroid/thyroid hormone receptor superfamily member in Drosophila melanogaster that shares extensive sequence similarity with a mammalian homologue. Nucleic Acids Res. 1990 Jul 25;18(14):4143–4148. [Europe PMC free article] [Abstract] [Google Scholar]
  • Hodin J, Riddiford LM. The ecdysone receptor and ultraspiracle regulate the timing and progression of ovarian morphogenesis during Drosophila metamorphosis. Dev Genes Evol. 1998 Aug;208(6):304–317. [Abstract] [Google Scholar]
  • Jowett T, Postlethwait JH. The Regulation of yolk polypeptide synthesis in Drosophila ovaries and fat body by 20-hydroxyecdysone and a juvenile hormone analog. Dev Biol. 1980 Nov;80(1):225–234. [Abstract] [Google Scholar]
  • Christianson AM, King DL, Hatzivassiliou E, Casas JE, Hallenbeck PL, Nikodem VM, Mitsialis SA, Kafatos FC. DNA binding and heteromerization of the Drosophila transcription factor chorion factor 1/ultraspiracle. Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11503–11507. [Europe PMC free article] [Abstract] [Google Scholar]
  • Koelle MR, Talbot WS, Segraves WA, Bender MT, Cherbas P, Hogness DS. The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily. Cell. 1991 Oct 4;67(1):59–77. [Abstract] [Google Scholar]
  • Bainbridge SP, Bownes M. Staging the metamorphosis of Drosophila melanogaster. J Embryol Exp Morphol. 1981 Dec;66:57–80. [Abstract] [Google Scholar]
  • Bender M, Imam FB, Talbot WS, Ganetzky B, Hogness DS. Drosophila ecdysone receptor mutations reveal functional differences among receptor isoforms. Cell. 1997 Dec 12;91(6):777–788. [Abstract] [Google Scholar]
  • Oro AE, McKeown M, Evans RM. Relationship between the product of the Drosophila ultraspiracle locus and the vertebrate retinoid X receptor. Nature. 1990 Sep 20;347(6290):298–301. [Abstract] [Google Scholar]
  • Bourguet W, Ruff M, Chambon P, Gronemeyer H, Moras D. Crystal structure of the ligand-binding domain of the human nuclear receptor RXR-alpha. Nature. 1995 Jun 1;375(6530):377–382. [Abstract] [Google Scholar]
  • Oro AE, McKeown M, Evans RM. The Drosophila retinoid X receptor homolog ultraspiracle functions in both female reproduction and eye morphogenesis. Development. 1992 Jun;115(2):449–462. [Abstract] [Google Scholar]
  • Parker MG, White R. Nuclear receptors spring into action. Nat Struct Biol. 1996 Feb;3(2):113–115. [Abstract] [Google Scholar]
  • Perrimon N, Engstrom L, Mahowald AP. Developmental genetics of the 2C-D region of the Drosophila X chromosome. Genetics. 1985 Sep;111(1):23–41. [Europe PMC free article] [Abstract] [Google Scholar]
  • Bownes M, Blair M, Kozma R, Dempster M. 20-hydroxyecdysone stimulates tissue-specific yolk-protein gene transcription in both male and female Drosophila. J Embryol Exp Morphol. 1983 Dec;78:249–268. [Abstract] [Google Scholar]
  • Brzozowski AM, Pike AC, Dauter Z, Hubbard RE, Bonn T, Engström O, Ohman L, Greene GL, Gustafsson JA, Carlquist M. Molecular basis of agonism and antagonism in the oestrogen receptor. Nature. 1997 Oct 16;389(6652):753–758. [Abstract] [Google Scholar]
  • Buszczak M, Freeman MR, Carlson JR, Bender M, Cooley L, Segraves WA. Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila. Development. 1999 Oct;126(20):4581–4589. [Abstract] [Google Scholar]
  • Renaud JP, Rochel N, Ruff M, Vivat V, Chambon P, Gronemeyer H, Moras D. Crystal structure of the RAR-gamma ligand-binding domain bound to all-trans retinoic acid. Nature. 1995 Dec 14;378(6558):681–689. [Abstract] [Google Scholar]
  • Hall BL, Thummel CS. The RXR homolog ultraspiracle is an essential component of the Drosophila ecdysone receptor. Development. 1998 Dec;125(23):4709–4717. [Abstract] [Google Scholar]
  • Handler AM, Maroy P. Ecdysteroid receptors in Drosophila melanogaster adult females. Mol Cell Endocrinol. 1989 May;63(1-2):103–109. [Abstract] [Google Scholar]
  • Schubiger M, Wade AA, Carney GE, Truman JW, Bender M. Drosophila EcR-B ecdysone receptor isoforms are required for larval molting and for neuron remodeling during metamorphosis. Development. 1998 Jun;125(11):2053–2062. [Abstract] [Google Scholar]
  • Handler AM, Postlethwait JH. Endocrine control of vitellogenesis in Drosophila melanogaster: effects of the brain and corpus allatum. J Exp Zool. 1977 Dec;202(3):389–402. [Abstract] [Google Scholar]
  • Shea MJ, King DL, Conboy MJ, Mariani BD, Kafatos FC. Proteins that bind to Drosophila chorion cis-regulatory elements: a new C2H2 zinc finger protein and a C2C2 steroid receptor-like component. Genes Dev. 1990 Jul;4(7):1128–1140. [Abstract] [Google Scholar]
  • Williams SP, Sigler PB. Atomic structure of progesterone complexed with its receptor. Nature. 1998 May 28;393(6683):392–396. [Abstract] [Google Scholar]
  • Yao TP, Segraves WA, Oro AE, McKeown M, Evans RM. Drosophila ultraspiracle modulates ecdysone receptor function via heterodimer formation. Cell. 1992 Oct 2;71(1):63–72. [Abstract] [Google Scholar]
  • Talbot WS, Swyryd EA, Hogness DS. Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms. Cell. 1993 Jul 2;73(7):1323–1337. [Abstract] [Google Scholar]
  • Yao TP, Forman BM, Jiang Z, Cherbas L, Chen JD, McKeown M, Cherbas P, Evans RM. Functional ecdysone receptor is the product of EcR and Ultraspiracle genes. Nature. 1993 Dec 2;366(6454):476–479. [Abstract] [Google Scholar]
  • Thomas HE, Stunnenberg HG, Stewart AF. Heterodimerization of the Drosophila ecdysone receptor with retinoid X receptor and ultraspiracle. Nature. 1993 Apr 1;362(6419):471–475. [Abstract] [Google Scholar]
  • Wagner RL, Apriletti JW, McGrath ME, West BL, Baxter JD, Fletterick RJ. A structural role for hormone in the thyroid hormone receptor. Nature. 1995 Dec 14;378(6558):690–697. [Abstract] [Google Scholar]
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NIGMS NIH HHS (2)