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Lecture 5 Slides rh
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Things to do with glucose… fig 14-1
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adenosine triphosphate… ATP
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fig 13-11
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free energies are additive!
11kg 68kg In metabolism, it is all about the coupling mechanism riddle: how do you lift 11 kg? ans: lower a weight > 11kg!
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fig 13-9
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It takes ATP to make ATP! the preparatory phase fig 14-2
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delayed gratification in glycolytic catabolism the payoff phase
fig 14-2
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Glucose catabolism around the bios… fig 14-3
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Phosphorylation of glucose
pg 532
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Hexokinse: an example of induced fit
fig 6-22
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Hexokinse: an example of induced fit
fig 6-22
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Isomerization of glucose 6-P
pg 532
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Anomers… rh
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The structural simplicity of isomerase reaction
rh
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Phosphorylation of fructose 6-P
pg 532
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Cleavage of fructose 1,6 bisphosphate by aldolase
pg 533
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Cleavage of fructose 1,6 bisphosphate by aldolase
now have two distinct 3-carbon molecules fig 14-6
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DHAP isomerization gives us 2 identical G3P
pg 534
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Isomerase action is familiar…
same chemistry, different enzyme
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Equivalent carbons from different parts of glucose
fig 14-6
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A fancy redox reaction…
pg 535
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A fancy redox reaction catalyzed by GAPDH
fig 14-7
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The GAPDH active site fig 14-7
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Enyzme-substrate complex
fig 14-7
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a covalent intermediate between a cysteine S and the substrate
fig 14-7
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The carbon molecule gets oxidized!! fig 14-7
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Phosphate as a nucleophile!!??
fig 14-7
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release of the product, 1,3 BPG fig 14-7
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Ready for more substrate…
fig 14-7
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This is the product of interest!
fig 14-7
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Voila! Our first ATP! XX pg 537
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Substrate level phosphorylation: the general idea
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“Isomerization” of 3PG to make 2PG
“mutase” is isomerase that moves groups pg 537
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Making an enol form by removal of H2O
pg 538
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A second substrate level phosphorylation: more ATP! pg 538
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Ta Da!!! Pyruvic acid pg 538
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LDH converts pyruvate to lactate, restoring the pool of NAD+ pg 547
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Glycolysis: energy without O2
neutrophil anaerobic organisms anaerobic situations
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CEOLACANTH: the Living Fossil
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Glycolysis in action: 100 mt sprint
Usain Bolt!!
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