ABSTRACT Hairy roots were obtained from snapdragon (Antirrhinum majus L.) plants infected with Ag... more ABSTRACT Hairy roots were obtained from snapdragon (Antirrhinum majus L.) plants infected with Agrobacterium rhizogenes harbouring the agropine type Ri plasmid (pRiA4). Plant regeneration occurred spontaneously on Linsmaier-Skoog (LS) medium lacking phytohormones under a 16-h/day photoperiod. Phenotypic alterations such as dwarfness, decrease in apical dominance, increase in number of flowers and stimulation of root production were observed in transformed plants. Opine analysis suggested the integration of TR-DNA and Southern analysis confirmed multiple integrations of TL-DNA. One of the transformed plants was fertile, and the progeny also exhibited the dwarf phenotype and had small flowers and elliptical leaves. Progeny expressing phenotypic alterations retained core T-DNA including rol genes.
Seventy-two transgenic plantlets were obtained from 18 hairy-root clones of prairie gentian (Eust... more Seventy-two transgenic plantlets were obtained from 18 hairy-root clones of prairie gentian (Eustoma grandiflorum (GRISEB-) SCHINNERS) transformed with Agrobacterium rhizogenes possessing the binary vector plasmid pBI121, which carried the neomycin phosphotransferase II (NPT II) and the β-glucuronidase (GUS) genes. Transformed hairyroot clones were selected on a medium containing kanamycin (km). Efficient and rapid plant regeneration occurred spontaneously on a selective medium without phytohormones. They conferred resistances to km and GUS activities. Polymerase Chain Reaction (PCR) and Southern analysis certified the existence of the NPT II and the GUS genes in the regenerated plants. Southern analysis confirmed that 1 to 7 copies were integrated into the plant genome.
Phylogenetic relationships within Clematis, including Naravelia, Archiclematis and Clematopsis, w... more Phylogenetic relationships within Clematis, including Naravelia, Archiclematis and Clematopsis, were analysed using nucleotide sequences of chloroplast DNA [(1) matK and trnK introns;(2) atpBrbcL spacer;(3) rpoBtrnC spacer;(4) psbAtrnHtrnQ spacer;(5) ...
ABSTRACT Hairy roots were obtained from snapdragon (Antirrhinum majus L.) plants infected with Ag... more ABSTRACT Hairy roots were obtained from snapdragon (Antirrhinum majus L.) plants infected with Agrobacterium rhizogenes harbouring the agropine type Ri plasmid (pRiA4). Plant regeneration occurred spontaneously on Linsmaier-Skoog (LS) medium lacking phytohormones under a 16-h/day photoperiod. Phenotypic alterations such as dwarfness, decrease in apical dominance, increase in number of flowers and stimulation of root production were observed in transformed plants. Opine analysis suggested the integration of TR-DNA and Southern analysis confirmed multiple integrations of TL-DNA. One of the transformed plants was fertile, and the progeny also exhibited the dwarf phenotype and had small flowers and elliptical leaves. Progeny expressing phenotypic alterations retained core T-DNA including rol genes.
Seventy-two transgenic plantlets were obtained from 18 hairy-root clones of prairie gentian (Eust... more Seventy-two transgenic plantlets were obtained from 18 hairy-root clones of prairie gentian (Eustoma grandiflorum (GRISEB-) SCHINNERS) transformed with Agrobacterium rhizogenes possessing the binary vector plasmid pBI121, which carried the neomycin phosphotransferase II (NPT II) and the β-glucuronidase (GUS) genes. Transformed hairyroot clones were selected on a medium containing kanamycin (km). Efficient and rapid plant regeneration occurred spontaneously on a selective medium without phytohormones. They conferred resistances to km and GUS activities. Polymerase Chain Reaction (PCR) and Southern analysis certified the existence of the NPT II and the GUS genes in the regenerated plants. Southern analysis confirmed that 1 to 7 copies were integrated into the plant genome.
Phylogenetic relationships within Clematis, including Naravelia, Archiclematis and Clematopsis, w... more Phylogenetic relationships within Clematis, including Naravelia, Archiclematis and Clematopsis, were analysed using nucleotide sequences of chloroplast DNA [(1) matK and trnK introns;(2) atpBrbcL spacer;(3) rpoBtrnC spacer;(4) psbAtrnHtrnQ spacer;(5) ...
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