[HTML][HTML] Effect of remote ischemic preconditioning in the expression of IL-6 and IL-10 in a rat model of liver ischemia-reperfusion injury
MAC Guimarães Filho, E Cortez… - Acta Cirurgica …, 2015 - SciELO Brasil
MAC Guimarães Filho, E Cortez, ÉP Garcia-Souza, VM Soares, AS Moura, L Carvalho…
Acta Cirurgica Brasileira, 2015•SciELO BrasilPURPOSE: To study the effect of remote ischemic preconditioning (RIPC) in ischemia-
reperfusion (I/R) liver injury and in the expression of IL-6 and IL-10 in a rat model.
METHODS: Thirty-six male rats were divided in three groups: Sham; I/R injury, a 45 minutes
lobar liver ischemia and reperfusion; and RIPC, six cycles of four minutes of ischemia and
four minutes of reperfusion on the right hindlimb followed by a 45 minutes lobar liver
ischemia and reperfusion. Tissue and blood samples were collected after 1h and 3h of …
reperfusion (I/R) liver injury and in the expression of IL-6 and IL-10 in a rat model.
METHODS: Thirty-six male rats were divided in three groups: Sham; I/R injury, a 45 minutes
lobar liver ischemia and reperfusion; and RIPC, six cycles of four minutes of ischemia and
four minutes of reperfusion on the right hindlimb followed by a 45 minutes lobar liver
ischemia and reperfusion. Tissue and blood samples were collected after 1h and 3h of …
PURPOSE
To study the effect of remote ischemic preconditioning (RIPC) in ischemia-reperfusion (I/R) liver injury and in the expression of IL-6 and IL-10 in a rat model.
METHODS
Thirty-six male rats were divided in three groups: Sham; I/R injury, a 45 minutes lobar liver ischemia and reperfusion; and RIPC, six cycles of four minutes of ischemia and four minutes of reperfusion on the right hindlimb followed by a 45 minutes lobar liver ischemia and reperfusion. Tissue and blood samples were collected after 1h and 3h of reperfusion for histopathological study, plasma cytokines and alanine aminotransferase (ALT) measurement.
RESULTS
The histopathological study demonstrated a significant reduction in liver necrosis in the RIPC group (p<0,001). The ALT levels were also significant lower in the RIPC group (p<0.01). The cytokines assessment showed that IL-6 levels were increased in the RIPC group after 1h of reperfusion, in comparison to the I/R group (p<0.05). Interleukin-10 levels in RIPC groups did not differ significantly from I/R group.
CONCLUSIONS
Remote ischemic preconditioning is effective in decreasing liver necrosis in a rat model of ischemia-reperfusion. The IL-6 expression is up-regulated and peaked at 60 min of reperfusion. There was no difference in IL-10 expression between the groups.
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