Calorimetric study of concanavalin A binding to saccharides
M Dani, F Manca, G Rialdi - Biochimica et Biophysica Acta (BBA)-Protein …, 1981 - Elsevier
The binding of concanavalin A in the dimer form to various saccharides has been studied by
calorimetry, and estimates of the binding enthalpy and binding constants have been
calculated. Methyl α-d-mannoside and methyl α-d-glucoside have a—ΔH 0 of 21.5 and 11.5
kJ/mol, respectively, at both pH 4 and 4.5. The p-nitrophenyl derivatives react with enthalpic
values of 15.6 and 14.6 kJ/mol. The galactosepyranosides show no heat effects during
mixing with the protein solutions. The apparent binding enthalpies calculated from the …
calorimetry, and estimates of the binding enthalpy and binding constants have been
calculated. Methyl α-d-mannoside and methyl α-d-glucoside have a—ΔH 0 of 21.5 and 11.5
kJ/mol, respectively, at both pH 4 and 4.5. The p-nitrophenyl derivatives react with enthalpic
values of 15.6 and 14.6 kJ/mol. The galactosepyranosides show no heat effects during
mixing with the protein solutions. The apparent binding enthalpies calculated from the …