Assisted reproductive technologies do not enhance the variability of DNA methylation imprints in human

S Tierling, NY Souren, J Gries, C LoPorto… - Journal of medical …, 2010 - jmg.bmj.com
Background Assisted reproductive technologies (ART) such as in vitro fertilisation (IVF) and
intracytoplasmic sperm injection (ICSI) are believed to destabilise genomic imprints. An …

Bi-PROF: bisulfite profiling of target regions using 454 GS FLX Titanium technology

J Gries, D Schumacher, J Arand, P Lutsik… - Epigenetics, 2013 - Taylor & Francis
The use of next generation sequencing has expanded our view on whole mammalian
methylome patterns. In particular, it provides a genome-wide insight of local DNA methylation …

Adult monozygotic twins discordant for intra-uterine growth have indistinguishable genome-wide DNA methylation profiles

NYP Souren, P Lutsik, G Gasparoni, S Tierling, J Gries… - Genome biology, 2013 - Springer
Background Low birth weight is associated with an increased adult metabolic disease risk. It
is widely discussed that poor intra-uterine conditions could induce long-lasting epigenetic …

Identification and analysis of methylation call differences between bisulfite microarray and bisulfite sequencing data with statistical learning techniques

M Döring, G Gasparoni, J Gries, K Nordström… - BMC …, 2015 - Springer
Background DNA methylation is an epigenetic modification known to play a prime role in
gene silencing and is an important topic in epigenetic research. However, due to technology-…

[PDF][PDF] Assisted reproductive technologies do not

S Tierling, NY Souren, J Gries - 2009 - researchgate.net
BACKGROUND: Assisted reproductive technologies (ART) such as in vitro fertilization (IVF)
and intracytoplasmic sperm injection (ICSI) are believed to destabilize genomic imprints. An …

DNA methylation studies on imprinted loci in a male monozygotic twin pair discordant for Beckwith–Wiedemann syndrome

S Tierling, NY Souren, S Reither, KD Zang… - Clinical …, 2011 - Wiley Online Library
Tierling S, Souren NY, Reither S, Zang KD, Meng‐Hentschel J, Leitner D, Oehl‐Jaschkowitz
B, Walter J. DNA methylation studies on imprinted loci in a male monozygotic twin pair …

Whole-genome bisulfite sequencing of two distinct interconvertible DNA methylomes of mouse embryonic stem cells

E Habibi, AB Brinkman, J Arand, LI Kroeze… - Cell stem cell, 2013 - cell.com
The use of two kinase inhibitors (2i) enables derivation of mouse embryonic stem cells (ESCs)
in the pluripotent ground state. Using whole-genome bisulfite sequencing (WGBS), we …

Selective impairment of methylation maintenance is the major cause of DNA methylation reprogramming in the early embryo

J Arand, M Wossidlo, K Lepikhov, JR Peat, W Reik… - Epigenetics & …, 2015 - Springer
Background DNA methylomes are extensively reprogrammed during mouse pre-implantation
and early germ cell development. The main feature of this reprogramming is a genome-…

In Vivo Control of CpG and Non-CpG DNA Methylation by DNA Methyltransferases

J Arand, D Spieler, T Karius, MR Branco… - PLoS …, 2012 - journals.plos.org
… We thank Jasmin Gries for performing the 454 sequencing, Mathias Bader for programming
the scripts for the analysis of Hairpin-bisulfite data, and Pascal Giehr and Valentina Perrera …

DNA methylation signatures of monozygotic twins clinically discordant for multiple sclerosis

NY Souren, LA Gerdes, P Lutsik, G Gasparoni… - Nature …, 2019 - nature.com
Multiple sclerosis (MS) is an inflammatory, demyelinating disease of the central nervous
system with a modest concordance rate in monozygotic twins, which strongly argues for …