The rat has been extensively used as a small animal model. Many genetically engineered
rat models have emerged in the last two decades, and the advent of gene-specific …

Reporter genes are widely applied in biotechnology and biomedical research owning to
their easy observation and lack of toxicity. Taking advantage of the reporter genes in …

Abstract Background The CRISPR/Cas9 system is increasingly used for gene inactivation in
mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less …

Regeneration of human kidneys in animal models would help combat the severe shortage of
donors in transplantation therapy. Previously, we demonstrated by interspecific blastocyst …

Historically, the rat has been the preferred animal model for behavioral studies. Limitations
in genome modification have, however, caused a lag in their use compared to the bevy of …

Murine animal models from genetically modified pluripotent stem cells (PSCs) are essential
for functional genomics and biomedical research, which require germline transmission for …

Genetic modification of pigs has many agricultural and biomedical applications. Ectopic
overexpression of foreign genes is necessary in many cases to generate transgenic pigs …

The CRISPR/Cas9 system has been used in a wide range of applications in the production
of gene-edited animals and plants. Most efforts to insert genes have relied on homology …

Since its domestication over 100 years ago, the laboratory rat has been the preferred
experimental animal in many areas of biomedical research (Lindsey and Baker The …

Functional assay limitations are an emerging issue in characterizing human pluripotent stem
cells (PSCs). With rodent PSCs, chimera formation using pre-implantation embryos is the …