[HTML][HTML] Exploitation of glycosylation in enveloped virus pathobiology

Y Watanabe, TA Bowden, IA Wilson… - Biochimica et Biophysica …, 2019 - Elsevier
Glycosylation is a ubiquitous post-translational modification responsible for a multitude of
crucial biological roles. As obligate parasites, viruses exploit host-cell machinery to …

Beyond shielding: the roles of glycans in the SARS-CoV-2 spike protein

L Casalino, Z Gaieb, JA Goldsmith, CK Hjorth… - ACS central …, 2020 - ACS Publications
The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) has resulted in more than 28,000,000 infections and 900,000 …

Meta-heterogeneity: evaluating and describing the diversity in glycosylation between sites on the same glycoprotein

T Čaval, AJR Heck, KR Reiding - Molecular & Cellular Proteomics, 2021 - ASBMB
Mass spectrometry–based glycoproteomics has gone through some incredible
developments over the last few years. Technological advances in glycopeptide enrichment …

Vulnerabilities in coronavirus glycan shields despite extensive glycosylation

Y Watanabe, ZT Berndsen, J Raghwani… - Nature …, 2020 - nature.com
Severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS)
coronaviruses (CoVs) are zoonotic pathogens with high fatality rates and pandemic …

DC/L-SIGN recognition of spike glycoprotein promotes SARS-CoV-2 trans-infection and can be inhibited by a glycomimetic antagonist

M Thépaut, J Luczkowiak, C Vivès, N Labiod… - PLoS …, 2021 - journals.plos.org
The efficient spread of SARS-CoV-2 resulted in a unique pandemic in modern history.
Despite early identification of ACE2 as the receptor for viral spike protein, much remains to …

[HTML][HTML] Variations within the glycan shield of SARS-CoV-2 impact viral spike dynamics

ML Newby, CA Fogarty, JD Allen, J Butler… - Journal of Molecular …, 2023 - Elsevier
The emergence of SARS-CoV-2 variants alters the efficacy of existing immunity, whether
arisen naturally or through vaccination. Understanding the structure of the viral spike assists …

Site-specific steric control of SARS-CoV-2 spike glycosylation

JD Allen, H Chawla, F Samsudin, L Zuzic… - Biochemistry, 2021 - ACS Publications
A central tenet in the design of vaccines is the display of native-like antigens in the elicitation
of protective immunity. The abundance of N-linked glycans across the SARS-CoV-2 spike …

Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimers as HIV-1 vaccine …

YN Zhang, J Paynter, A Antanasijevic, JD Allen… - Nature …, 2023 - nature.com
Uncleaved prefusion-optimized (UFO) design can stabilize diverse HIV-1 envelope
glycoproteins (Envs). Single-component, self-assembling protein nanoparticles (1c-SApNP) …

Site-specific glycosylation of virion-derived HIV-1 Env is mimicked by a soluble trimeric immunogen

WB Struwe, E Chertova, JD Allen, GE Seabright… - Cell reports, 2018 - cell.com
Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the
glycan shield on native, virion-associated envelope glycoprotein (Env) spikes. The same …

Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens

ML Newby, JD Allen, M Crispin - Biotechnology Advances, 2024 - Elsevier
A key aspect of successful viral vaccine design is the elicitation of neutralizing antibodies
targeting viral attachment and fusion glycoproteins that embellish viral particles. This …