Members of the genus Bifidobacterium are among the first microbes to colonize the human
gastrointestinal tract and are believed to exert positive health benefits on their host. Due to …

This mini-review provides a perspective of traditional, emerging and future applications of
lactic acid bacteria (LAB) and how genome editing tools can be used to overcome current …

While lactic acid producing fermentation has been utilized to improve the storability,
palatability, and nutritive value of perishable foods for a very long time, only recently have …

The strong restriction barrier present in Staphylococcus aureus and Staphylococcus
epidermidis has limited functional genomic analysis to a small subset of strains that are …

Antibiotic therapy often fails to eliminate a fraction of transiently refractory bacteria, causing
relapses and chronic infections. Multiple mechanisms can induce such persisters with high …

A system for high-efficiency single-and double-crossover homologous integration in gram-
positive bacteria has been developed, with Lactococcus lactis as a model system. The …

In lactococci, the study of chromosomal genes and their regulation is limited by the lack of an
efficient transposon mutagenesis system. We associated the insertion sequence ISS1 with …

The intracellular bacterium Listeria monocytogenes can invade several types of normally
non‐phagocytic cells. Entry into cultured epithelial cells requires the expression of inIA, the …

Most invasive bacterial infections are caused by species that more commonly colonize the
human host with minimal symptoms. Although phenotypic or genetic correlates underlying a …

A system for generating chromosomal insertions in lactococci is described. It is based on the
conditional replication of lactococcal pWV01-derived Ori+ RepA-vector pORI19, containing …