Nothing Special   »   [go: up one dir, main page]

Effects of Bacillus subtilis on some physiological and biochemical parameters of Triticum aestivum L. (wheat) under salinity

Plant Physiol Biochem. 2017 Dec:121:80-88. doi: 10.1016/j.plaphy.2017.10.020. Epub 2017 Nov 6.

Abstract

Endophytic strain Bacillus subtilis (B. subtilis) 10-4, producing indole-3-acetic acid (IAA) and siderofores but not active in phosphate solubilization, exerted a protective effect on Triticum aestivum L. (wheat) plant grown under salinity (2% NaCl) stress. Exposure to salt stress resulted in an essential increase of proline (Pro) and malondialdehyde (MDA) level in the seedlings. At the same time the seedlings inoculated with B. subtilis 10-4 were characterized by decreased level of stress-induced Pro and MDA accumulation. It was revealed that both B. subtilis 10-4 and salinity caused increase in the content of endogenous salicylic acid (SA) in wheat seedlings as compared to SA content in the control, while B. subtilis 10-4 suppressed stress-induced SA accumulation. Water storage capacity (WSC) in leaf tissues was increased and stress-induced hydrolysis of statolite starch in root cap cells of the germinal roots was reduced by B. subtilis 10-4. The obtained data indicated that the activation of the defense reactions induced by B. subtilis 10-4 induced defense reactions may be connected with their ability to decrease the level of stress-induced oxidative and osmotic stress in seedlings and with the increase of endogenous SA level that can make a significant contribution to the implementation of the protective effect of B. subtilis 10-4 and is manifested in the improvement of plant growth, WSC of leaves and slowing down of the process of statolite starch hydrolysis under salinity.

Keywords: Bacillus subtilis; Malondialdehyde; Salicylic acid; Salinity; Triticum aestivum L.; Water storage capacity; proline.

MeSH terms

  • Bacillus subtilis / physiology*
  • Osmotic Pressure*
  • Plant Root Cap / metabolism*
  • Salinity*
  • Seedlings / metabolism*
  • Seedlings / microbiology
  • Triticum / metabolism*
  • Triticum / microbiology