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Infection with MERS-CoV causes lethal pneumonia in the common marmoset

PLoS Pathog. 2014 Aug 21;10(8):e1004250. doi: 10.1371/journal.ppat.1004250. eCollection 2014 Aug.

Abstract

The availability of a robust disease model is essential for the development of countermeasures for Middle East respiratory syndrome coronavirus (MERS-CoV). While a rhesus macaque model of MERS-CoV has been established, the lack of uniform, severe disease in this model complicates the analysis of countermeasure studies. Modeling of the interaction between the MERS-CoV spike glycoprotein and its receptor dipeptidyl peptidase 4 predicted comparable interaction energies in common marmosets and humans. The suitability of the marmoset as a MERS-CoV model was tested by inoculation via combined intratracheal, intranasal, oral and ocular routes. Most of the marmosets developed a progressive severe pneumonia leading to euthanasia of some animals. Extensive lesions were evident in the lungs of all animals necropsied at different time points post inoculation. Some animals were also viremic; high viral loads were detected in the lungs of all infected animals, and total RNAseq demonstrated the induction of immune and inflammatory pathways. This is the first description of a severe, partially lethal, disease model of MERS-CoV, and as such will have a major impact on the ability to assess the efficacy of vaccines and treatment strategies as well as allowing more detailed pathogenesis studies.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Callithrix
  • Coronavirus Infections / pathology*
  • Coronavirus Infections / virology
  • Disease Models, Animal*
  • Immunohistochemistry
  • Male
  • Middle East Respiratory Syndrome Coronavirus
  • Pneumonia, Viral / pathology*
  • Pneumonia, Viral / virology
  • Real-Time Polymerase Chain Reaction