Abstract
We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Coronavirus 229E, Human / genetics
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Coronavirus 229E, Human / isolation & purification
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Coronavirus Infections / diagnosis
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Coronavirus Infections / genetics
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Coronavirus Infections / virology*
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Coronavirus NL63, Human / genetics
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Coronavirus NL63, Human / isolation & purification
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Coronavirus OC43, Human / genetics
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Coronavirus OC43, Human / isolation & purification
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Humans
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Open Reading Frames
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Real-Time Polymerase Chain Reaction / methods*
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Saudi Arabia
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Sensitivity and Specificity
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Severe acute respiratory syndrome-related coronavirus / genetics*
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Severe acute respiratory syndrome-related coronavirus / isolation & purification*
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Travel
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Viral Envelope Proteins
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Viroporin Proteins
Substances
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E protein, SARS coronavirus
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Viral Envelope Proteins
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Viroporin Proteins