Abstract
A parallel microfluidic cytometer (PMC) uses a high-speed scanning photomultiplier-based detector to combine low-pixel-count, one-dimensional imaging with flow cytometry. The 384 parallel flow channels of the PMC decouple count rate from signal-to-noise ratio. Using six-pixel one-dimensional images, we investigated protein localization in a yeast model for human protein misfolding diseases and demonstrated the feasibility of a nuclear-translocation assay in Chinese hamster ovary (CHO) cells expressing an NFκB-EGFP reporter.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Active Transport, Cell Nucleus
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Algorithms
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Animals
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CHO Cells
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Cricetinae
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Cricetulus
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Flow Cytometry / instrumentation*
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Flow Cytometry / statistics & numerical data
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Green Fluorescent Proteins / chemistry
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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High-Throughput Screening Assays / instrumentation
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High-Throughput Screening Assays / statistics & numerical data
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Humans
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Image Processing, Computer-Assisted / statistics & numerical data
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Microfluidic Analytical Techniques / instrumentation*
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Microfluidic Analytical Techniques / statistics & numerical data
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NF-kappa B / genetics
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NF-kappa B / metabolism
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Protein Multimerization
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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alpha-Synuclein / chemistry
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alpha-Synuclein / genetics
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alpha-Synuclein / metabolism
Substances
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NF-kappa B
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Recombinant Fusion Proteins
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alpha-Synuclein
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enhanced green fluorescent protein
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Green Fluorescent Proteins