Nothing Special   »   [go: up one dir, main page]

Local transient induction of inflammatory cytokines after intranasal administration of recombinant Bordetella pertussis

Microb Pathog. 1997 May;22(5):305-13. doi: 10.1006/mpat.1996.0130.

Abstract

Inflammatory cytokines have been described to play a critical role in the orientation and amplification of the IgA immune response. In this study, we show that the intranasal administration of a Bordetella pertussis strain expressing the protective antigen glutathione-S-transferase of Schistosoma mansoni (Sm28GST) induced an inflammatory response in the lungs of mice, characterized by the production of inflammatory cytokines, such as Tumor Necrosis Factor alpha, Interleukin-6 and Transforming-Growth Factor beta. The production and the secretion of these cytokines in lung tissues were early and transient. Their presence was observed only during the first week after administration despite the persistence of the bacteria for 1 month. Two weeks after inoculation, Interleukin-10 secretion was detected in the lungs, which could explain the decrease in the production of inflammatory cytokines. These inflammation-regulating cytokines, induced in the lungs by the presence of the bacterial vector, could be part of the process generating the local immune response, in particular the anti-Sm28GST IgA response.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intranasal
  • Animals
  • Antigens, Helminth / immunology*
  • Bordetella pertussis*
  • Bronchoalveolar Lavage
  • Female
  • Genetic Vectors*
  • Glutathione Transferase / immunology*
  • Inflammation
  • Interleukin-10 / analysis*
  • Interleukin-6 / analysis*
  • Lung / immunology
  • Lung / pathology
  • Mice
  • Mice, Inbred BALB C
  • Recombination, Genetic
  • Schistosoma mansoni / enzymology*
  • Transforming Growth Factor beta / analysis*
  • Tumor Necrosis Factor-alpha / analysis*

Substances

  • Antigens, Helminth
  • Interleukin-6
  • Transforming Growth Factor beta
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • Glutathione Transferase