Nothing Special   »   [go: up one dir, main page]
Skip to main content
. Author manuscript; available in PMC: 2014 Dec 20.
Published in final edited form as: ACS Chem Biol. 2013 Oct 28;8(12):2744–2752. doi: 10.1021/cb400612h

Table 1.

Kinetic properties of NS2B-NS3pro variants.

Variant KM
(µM)		 kcat
(min−1)		 kcat/KM
(min−1/µM)		 Molar Ratio
of DTNB
binding		 Molar Ratio
of BAClMK
binding
wild type 333 ± 21 1.5 ± 0.001 4.5 × 10−3 0 0
E19C 321 ± 39 1.0 ± 0.10 3.1 × 10−3 1 1
L31C 343 ± 26 1.0 ± 0.23 2.9 × 10−3 1 0.8
W83C N.D. N.D. - 0.2 N.T.
E86C 290 ± 51 0.8 ± 0.20 2.7 × 10−3 1 1
N105C 271 ± 23 1.6 ± 0.03 5.9 × 10−3 1 1
T111C N.D. N.D. - 1 N.T.
L115C N.D. N.D. - 1 N.T.
A125C 235 ± 45 1.1 ± 0.30 4.6 × 10−3 1 1

N.D. - not detectable; N.T. – not tested