Simple Methods for Generating and Detecting Locus-Specific Mutations Induced with TALENs in the Zebrafish Genome

Design and construction of TALENs

The TALENs we generate function as sequence-specific heterodimer endonucleases. Each monomer component is a chimeric protein composed of the FokI nuclease domain fused with a synthetic DNA binding domain consisting of an array of RVD repeat modules. Nuclease activity requires the binding of the two components on opposing strands of the duplex at a small interval distance. Although the FokI enzyme normally functions as a homodimer, we utilize mutant derivatives of the nuclease domain [23] so that the TALENs function as obligate heterodimers, thus demanding that both ‘Left’ and ‘Right’ monomers simultaneously recognize their cognate binding sites to achieve nuclease activity. The RVD repeat assembly reagents generated by Cermak et al. [30] allow construction of sequences encoding DNA binding domains composed of up to 31 recognition motifs. However, generally we design monomer TALEN components that each contain 16–20 RVD repeats and that, including the DNA interaction function of the N-terminal portion of the TALEN [28], [29], bind a half-target site of approximately 17–21 nt present on opposing strands and spaced apart by 14–17 bp (target site configuration ≥17 bp – N_14–17 – ≥17 bp). Applying parameters described in Materials and Methods to the TALEN Targeter program (https://boglab.plp.iastate.edu/node/add/talen), TALENs can be designed that recognize only a single target site in the zebrafish genome. Such unique target sites can be identified in many exons, as well as introns and promoter sequences (see Discussion). The gene sequences targeted and the RVD repeat arrays of the TALENs used in this study are presented in Figure S1.

To generate a plasmid encoding a Left or Right monomer component consisting of n RVD repeats (see Materials and Methods for details), an initial Golden Gate cloning step is used to assemble two arrays, encoding repeats 1–10 and repeats 11 – n-1, as in [30]. The final expression plasmid encoding an entire TALEN monomer is generated in a second Golden Gate cloning assembly, which brings together the two partial arrays, sequences encoding the n^th motif, and a modified CS2⁺ backbone vector, pCS2TAL3RR or pCS2TAL3DD (Figure S2; see Materials and Methods). Assembly results in a fusion gene that encodes: vector-provided N-terminal TALE-derived sequences, an RVD repeat array, C-terminal TALE-derived sequences, and a modified nuclease domain. TALENs can be expressed directly from the CMV promoter resident in these CS2⁺ vectors. However, for the zebrafish experiments described below, mRNA encoding Left or Right monomer components were generated individually by in vitro transcription of linearized plasmids and equal amounts of each mRNA were co-injected into embryos at the 1 cell stage.

Induction of somatic mutations at the golden locus

To estimate the efficiency with which targeted mutations can be induced, we measured the ability of TALENs to induce somatic tissue mutations in the golden (gol) gene, which governs pigmentation in the embryo and adult without compromising viability [33], [34]. Mutations at gol are recessive: embryos with at least one WT allele are darkly pigmented at 2 days postfertilization (dpf), whereas homozygous gol mutants appear pigmentless at this stage. To maximize the chance of inducing complete loss-of-function alleles, we chose to develop a TALEN that would generate DSBs within coding sequences residing toward the 5′ end of the gol locus. Using criteria described in Materials and Methods, a potential TALEN target site was identified in the second exon of gol and the gol-ex2 TALEN was designed (Figure 1A, Figure S1). As imprecise repair of targeted DSBs is likely to produce recessive loss-of-function mutations at gol, we injected embryos heterozygous for the gol^b1 null mutation [33], [34] with gol-ex2 TALEN mRNA and measured the appearance of gol^b1/* mutant pigmentless cells in the Retinal Pigmented Epithelium (RPE) (Figure 1). At 2 dpf the RPE is a monolayer of approximately 550 pigmented cells that envelops each eye, and the presence of even small clones of pigmentless tissue can be detected easily [35]. Whereas it is extremely rare for pigmentless cells to be found in the RPEs of control gol^b1/+ heterozygous embryos [36], all but one of 20 TALEN-injected gol^b1/+ embryos had large patches of gol mutant cells (Figure 1B–1F, Table 1). The TALEN-injected embryos had multiple patches of mutant tissue, indicating they were genetically mosaic.

On average ≥50% of the RPE cells in TALEN RNA-injected embryos were golden (Figure 1C–1F), indicating the majority of genomes in the embryos acquired TALEN-induced mutations. Three experiments demonstrated the new mutations were indeed induced by the gol-ex2 TALEN. First, the gol-ex2 TALEN could induce pigmentless tissue in the RPEs of gol^+/+ embryos (Figure 1G–1K), indicating the induction of mutant tissue did not require a pre-existing gol mutant allele. Mutant cells were observed in almost 100% of these injected WT embryos (Table 1) and occasionally even wholly gol embryos were observed (Figure 1L, 1M; see Table S2 for frequencies), highlighting the efficiency with which these TALENs can induce mutations in both genomes of a cell. Second, as discussed below, analysis of exon 2 sequences amplified from gol-ex2 TALEN RNA-injected embryos revealed a diverse set of indel mutations were induced, typical of those produced by NHEJ-mediated repair of DSBs. Third, gol mutant alleles were transmitted to the F1 offspring of TALEN-injected WT embryos (see below). We conclude the gol-ex2 TALEN is extremely effective at inducing mutations at gol.

A general strategy for detecting mutations induced with TALENs

Whereas newly induced mutations at golden are simple to detect, TALEN-induced mutations at most loci are unlikely to present a phenotype that can be scored in individual somatic cells. As the repair of DSBs can lead to an assortment of indels centered at the TALEN target site, it is desirable to detect induced mutations with a method that can detect any DNA change arising at a selected target site. Furthermore, as DSBs are induced only following translation of injected TALEN mRNAs, an individual embryo may acquire several independent mutations, each of which may arise in a mosaic fashion during development. Therefore a sensitive method is required that can detect mixtures of TALEN-induced DNA polymorphisms at a targeted locus present among the genomes of a single embryo.

We find High Resolution Melt Analysis (HRMA) is a simple, rapid, and sensitive method for detecting TALEN-induced somatic mutations. HRMA has been used in the past to detect known sequence polymorphisms in the zebrafish [37], but it is also useful for discovering polymorphisms of unknown sequence that lie in a small, defined region of the genome. We developed standard conditions for detecting sequence alterations resulting from TALEN activity at targeted loci (Materials and Methods). To detect the occurrence of a DNA polymorphism at a particular locus, short PCR amplicons (90–120 bp) that include the region of interest are generated from a genomic DNA (gDNA) sample, subjected to denaturation and rapid renaturation, and the thermostability of the population of renatured amplicons is analyzed (Materials and Methods). If TALEN-induced polymorphisms are present in the template gDNA, heteroduplex as well as different homoduplex molecules will be formed (Figure 2A). The presence of multiple forms of duplex molecules is detected by HRMA, which records the profile of temperature-dependent denaturation and detects whether duplex melting acts as a single species or more than one species. For example, three prominent duplex types with distinct melting temperatures (Tm's) are evident in the analysis of renatured lef1 amplicons generated from an embryo heterozygous for an intragenic 18 bp deletion in lef1 (Figure 2B).

We determined the sensitivity of HRMA for detecting the presence of a mutant genome mixed among a population of WT genomes using standard conditions of analysis (Materials and Methods). gDNA prepared from an embryo heterozygous for the lef1^Δ18 mutation was mixed with differing amounts of WT gDNA, and the ability of HRMA to detect the mutant allele was determined. As the mutant genome is present as a decreasing fraction of all template genomes, the relative abundance among amplicons of homoduplex mutant and heteroduplex populations changes, but the presence of mutant genomes can be detected unambiguously even when mutant genomes represent 1/70^th of the total mix (Figure 2C). Figure S3 shows a 4 bp insertion mutation in the lef1 gene can be detected with similar sensitivity. In genetically mosaic embryos, when multiple mutant alleles are present as minor populations within a mixture of genome, the melt curves become more complex, but deflections away from the WT profile are additive and therefore simple to detect (see examples in Figure 3). Given our findings (presented below) that the majority of mutations induced using the methods described here consist of alterations of >4 bp, we estimate our standard conditions of analysis can routinely detect one mutant genome present among 50 WT genomes.

HRMA reveals TALENs induce mutations efficiently at many loci

To test the efficacy of our methods for generating and detecting TALEN-induced somatic mutations at any locus, we injected 1 cell embryos with mRNAs encoding the gol-ex2 TALEN or TALENs designed to recognize and cleave sequences in exon 3 of tbx6, exon 5 of ryr3, or exon 6 of ryr1a (Figure S1). Under standard conditions of injection with 100 pg total TALEN RNA, approximately 95% of the embryos developed normally (Table S3). gDNA was prepared from individual 1–2 dpf TALEN-injected or control embryos and analyzed by HRMA for the presence of DNA sequence variants at the targeted loci using primer pairs listed in Table S1. Nearly all TALEN RNA-injected embryos had targeted mutations, including the gol-ex2 TALEN RNA-injected embryos that did not have gol mutant cells in the RPE (Table 2, Figure 3). Sequence analysis of PCR amplicons covering the targeted loci (Figure 3) indicated the induction of a spectrum of indel mutations, consistent with what is expected from NHEJ repair. Analysis of loci amplified from 9 embryos targeted at tbx6, ryr3, or ryr1a indicated approximately half the genomes (26/52) of TALEN-injected embryos harbored targeted mutations.

Dose-dependent induction of mutations

As gol does not encode an essential function, the induction of gol mutant cells is not detrimental to development. However for other targeted genes it may be advantageous to avoid inducing bi-allelic mutations in many cells of the embryo. The frequency with which mutant cells are induced is dependent on the amount of TALEN RNA that is injected (Table 1, Table S2), so the frequency of cells harboring bi-allelic mutations can be adjusted. As shown in Figure 4, HRMA can be used to determine the dose-dependent induction of mutations that cannot be easily measured in somatic tissues. The amount of TALEN RNA delivered to embryos affects both the fraction of embryos that harbor detectable mutations as well as the average abundance of mutant genomes present in any individual injected embryo.

Recovery of TALEN–induced germ line mutations

To determine if induced mutations detected in 1–2 dpf embryos enter the germ line, we raised TALEN-injected (G0) embryos to adulthood and analyzed the transmission of mutations to progeny. Individual adult G0 animals were mated with WT partners and gDNA isolated from individual F1 embryos was analyzed by HRMA. Targeted germ line mutations were transmitted by 51 of the 57 G0 animals (approximately 90%) that had been exposed to TALENs directed at the golden, tbx6, ryr3, or ryr1a genes (complete transmission data is provided in Tables S4, S5, S6, S7; data is summarized in Table 3). The fraction of G0s carrying germ line mutations induced by each TALEN ranged from 77% to 100%. Analysis of the sequence alterations inherited by F1 embryos revealed the range of induced indel mutations among the germ line transmitted mutations mimicked those that had been observed in embryos (Figure 5A). The majority of TALEN-induced mutations identified here in injected G0, 1–2 dpf F1, or adult F1 individuals were sequence changes of 3–20 bp (Figure 3, Figure 5, Table S8). Larger indels were identified, but rarely. Most if not all mutations identified among the 1 dpf F1 offspring were viable in the heterozygous state, as adult F1s descended from G0 founders harbored a distribution of mutations similar to that found among the F1 embryos (Table S8). To date all the F1 fish heterozygous for TALEN-induced mutations at tbx6, ryr1a, and ryr3 that have been bred (n = 16) have transmitted their mutations to the F2 generation. We have no evidence of mutations in the F1 generation that could not be further propagated.

Most G0 animals transmitted multiple mutant alleles and most mutations were transmitted by significantly less than 50% of the gametes (Table 3; Tables S4, S5, S6, S7, S8), indicating the germ lines of G0 founders were mosaic. HRMA analysis of heterozygous F1 offspring was used to distinguish transmitted mutant alleles, and sibling individuals that appeared to carry a common DNA sequence alteration were grouped based on common melt curve shapes. As illustrated in Figure 5B and 5C, individual sibling F1 embryos descended from a single mutagenized G0 founder could harbor different alleles, indicated by the multiple distinct HRMA melting profiles of amplicons derived from sibling F1 offspring. On average, two new alleles were recovered from the germ lines of each mutagenized G0 founder (Table 3).

The finding that the germ lines of most G0 founders were genetically mosaic is consistent with the interpretation that TALENs induce mutations independently in different cells of the embryo and mutations accrue as the embryo develops. Indeed we found that following injection of 1 cell stage embryos with gol-ex2 TALEN mRNAs, the fraction of embryos with detectable levels of targeted mutations increased with developmental time (Figure S4). Whereas 100% of injected embryos had mutations at 6 or 24 hours postfertilization (hpf), only a fraction of the 3 hpf embryos had detectable mutations, and among the early stage embryos, HRMA genotype analyses indicated a relatively low abundance of mutant genomes (Figure S4). Altogether these data indicate numerous targeted indel mutations can be recovered routinely from a small set of adults arising from TALEN mRNA-injected embryos.

TALENs demonstrate target locus specificity

The very high frequency with which mutations were induced raised the possibility that the TALENs were simply mutagenic in a sequence-non-specific manner. However, in contrast with published reports [21], [22], [23] and our own experience with ZFNs (data not shown), TALENs exhibit very low toxicity. For example, although injection of only 4 pg total RNA encoding any of the TALENs used here was sufficient to induce mutations in the majority of embryos (Figure 4 and data not shown), approximately 95% of the embryos developed normally even after being injected with 100 pg total TALEN RNA (Table S3). These observations are consistent with the interpretation that random chromosome breaks are generally not produced by the TALENs.

As one test of the specificity of the TALENs used here, we generated TALENs to recognize a specific target sequence and measured the induction of mutations at very closely related sequences present in homologues of the targeted gene. The ryr genes encode related Ryanodine Receptor intracellular calcium release channels. We designed TALENs targeted to the ryr3 or ryr1a genes and measured the induction of mutations in the homologous sequences present in the ryr1a, ryr1b, or ryr3 genes. The ryr3-ex5 TALEN recognizes a Left half-site of 19 nt and Right half-site of 18 nt (Figure S1). As illustrated in Figure 6A, the homologous sites in the ryr1a and ryr1b genes present a 3 base mismatch for the Left monomer and 4 base mismatch for the Right monomer of the ryr3-ex5 TALEN. Although HRMA indicated every embryo injected with ryr3-ex5 TALEN had induced mutations in ryr3, the embryos did not have detectable levels of mutations at the ryr1a or ryr1b loci (Figure 6B).

In a second experiment we analyzed the off-target activity of the ryr1a-ex6 TALEN, which recognizes a Left half-site of 19 nt and Right half-site of 17 nt. As shown in Figure 6C, the homologous site in ryr3 differs at 3 positions in both the Left and the Right half-sites, but the homologous site in ryr1b differs only at 2 positions in the Left half-site and presents a perfect match with the Right half-site. HRMA analysis revealed the ryr1a-ex6 TALEN induced targeted mutations at the cognate locus in 100% of the injected embryos but failed to induce detectable mutations at the homologous ryr3 target (Figure 6D). In contrast, the ryr1a-ex6 TALEN did induce mutations in ryr1b gene of all of the injected embryos (Figure 6D). These results indicate the TALENs display high but not perfect sequence specificity under the conditions described here.

Overview and implications

Sequence-specific TALENs that can target almost any zebrafish gene can be generated simply and rapidly using the target site design parameters, the reagents, and the cloning strategy described here. The TALENs generated using these reagents are very effective at inducing DSBs whose repair often produces mutations at the target sites. Using our standard methods to generate mutations at four different loci, we found virtually every embryo injected with TALEN mRNA harbored targeted mutations. As demonstrated with the gol-ex2 TALEN, in some cases over half the genomes in a TALEN RNA-injected embryo may acquire mutations at targeted loci. Almost all treated animals acquire new germ line mutations, which are subsequently inherited in a stable Mendelian fashion. We have found many additional sites in the genome can be targeted with the ease and efficiency of the four genes described here: during the course of the current study we induced mutations at 19 of the 21 sites we attempted to target (90%). The genes that have been successfully targeted are present at many different locations in the genomes (Figure S1) including the gol locus, which is close to a telomere [33]. Further, as shown by the induction of gol mutations in early stage embryos (Figure S4), mutations can be induced in genes that are not being expressed. Given the modular mode of RVD repeat module binding, the flexibility in the design and the length of repeat arrays that can be generated, and the relatively few guidelines that restrict TALEN design, it appears TALENs can be used to induce loss-of-function mutations in almost every zebrafish gene.

The system we present here for constructing TALENs that are effective in the zebrafish is easy to implement: 1) it employs a very simple and rapid cloning strategy, using the Golden Gate cloning method system to assemble RVD repeats; 2) the RVD repeats are available in plasmids that can be used directly for Golden Gate cloning without need to PCR amplify or otherwise alter the repeat sequences; 3) the cloning strategy leads to accurate constructions of arrays (we typically analyze only 2 transformants from each cloning step); 4) the TALENs function as obligate heterodimers, increasing specificity of target sites recognized by the TALENs; and 5) all reagents are readily available through Addgene.

Although the studies presented here focus on induction of mutations in the zebrafish, our preliminary results indicate that TALENs assembled using the reagents described here are also effective at inducing mutations in Drosophila and mammalian cell culture systems.

We developed High Resolution Melt Analysis as a principal method for measuring the induction of mutations with TALENs in zebrafish embryos. HRMA can detect almost any newly arising polymorphism at a pre-specified region of the genome, and thus it allows detection of the large variety of sequence changes that may be produced by NHEJ repair. As HRMA can detect sequence alterations arising at any target site, this method of mutation detection does not bias or affect the choice of a target site and thus is an improvement over previous assays that detected loss of a restriction site. We show HRMA is an extremely sensitive method for detecting mutant genomes among a mixed population of genomes. Hence it is particularly useful for detecting TALEN-induced mutations, any one of which is likely to be present in only a subset of the cells of an embryo injected with TALEN RNAs. Furthermore, because HRMA is sensitive to the total fraction of mutant genomes in a mixture, it is particularly well-suited for detecting the heterogeneous set of mutations that is likely to arise in a single embryo. Finally, HRMA is simple to apply and does not involve manipulation of samples following PCR amplification. The entire procedure for generating amplicons and analyzing the thermostability of the heteroduplexes can be performed in less than 2 hours.

Practical aspects of the use of TALENs to generate locus-specific mutations in zebrafish

We consider several practical issues concerning implementation of the methods presented here to induce mutations in the zebrafish: 1) the kinds of mutations generated and implications for target design considerations; 2) the frequency with which potential TALEN target sites can be identified; 3) the possibility that TALEN-injected embryos express mutant phenotypes; and 4) the ability of TALENs to induce unintended mutations.

Most of the mutations we have recovered from G0 germ lines are indels that affect ≤20 bp stretches of genomic sequence. Many of these sequence changes cause frameshift mutations. If TALENs are used with the goal of inducing null mutations in a targeted gene, it is best to target a region of the gene in which a frameshift mutation is likely to produce a protein product that lacks important functional elements. We routinely target the second or third exon of a gene. Among 40 genes for which we have designed TALENs, we have always been able to identify targets in the second and/or third exons of the genes using the design parameters presented in the Materials and Methods. Among searches of 116 stretches of (mostly coding) sequences with an average size 238 bp, we identified at least one suitable target in 77% of the sequences (the average size of the sequences without a best-fit target was 158 bp). Using the guidelines we suggest, optimal TALEN target sites can be identified for most genes.

The parameters that govern the specificity and activity of TALENs are not completely understood. It is clear from our studies and those of others that the Left and Right TALEN monomer components can bind at various distances from each other and still cooperate effectively to accomplish target site cleavage [27]. The spacer length for achieving optimal activity has not been determined, and we can only say the 14–17 bp spacer length we routinely use in the design of TALEN target sites consistently allows for effective target cleavage in the zebrafish. Importantly, the extreme minimum or maximum spacer distance at which some cleavage activity may occur has yet to be determined in vivo, an uncertainty that affects the identification of unintended sites in the genome that may be susceptible to TALEN activity.

As expected from previous work on the specificity and selectivity of TALE binding [25], [27], the TALENs function as highly sequence-specific nucleases. Given that we identify potential TALEN target sites on the basis of a reference genome, and as the common laboratory WT strains of zebrafish harbor polymorphisms, we have found it prudent to sequence genomic regions of the zebrafish used in any series of experiments to verify the existence of a presumed target site in the embryos that are injected with TALEN RNA. Furthermore, it should be noted HRMA can detect pre-existing polymorphisms, and thus we choose to inject embryos shown to be free of polymorphisms near the TALEN target site.

We routinely verify the in vivo activity of a TALEN before growing injected G0 embryos to adulthood. Typically we inject 1 cell stage embryos with different amounts (4 pg, 20 pg, 100 pg total RNA) of a 1∶1 mixture of Left TALEN and Right TALEN RNAs and measure the presence of mutations in individual 1 dpf embryos. In our experience to date, most animals that have evidence of mutations at 1 dpf will grow to become adults that transmit mutations through the germ line.

As demonstrated with the gol-ex2 TALENs, cells with two mutant alleles can be induced following injection of 1 cell embryos with TALEN RNA. Mutations are induced in a mosaic fashion and using the conditions described here, it is rare that TALEN-injected embryos exhibit strong mutant phenotypes. It may be possible to augment the activity of the TALENs to uncover mutant phenotypes in G0 animals. In addition, it is worth considering the possibility that some mutations cannot survive in the germ line in a homozygous state. As a result, it may be desirable to raise G0 animals with sub-maximal levels of mutations. As demonstrated in our studies, the frequency of TALEN-induced mutations is a function of the amount of TALENs introduced into an animal. Thus, it is possible to raise animals that carry different mutation loads.

Finally, although we have not made extensive measurements of the frequency with which unintended sequences are recognized and cleaved by TALENs in these experiments, our studies indicate off-target mutations can occur but they are sufficiently infrequent so that they are unlikely to confound analysis of targeted gene function. We found TALENs failed to cleave potential target sites that differed at about 6 positions of a 36 nt binding target, but that targets differing at only 2 positions could be effectively cleaved. The effects of off-target mutations can be minimized by studying hetero-allelic combinations of targeted mutations derived from independently mutagenized G0 founders. In sum, the current conditions for TALEN-induced mutagenesis appear sufficient for uncovering the function of almost any selected gene in the zebrafish.

Practical aspects of the use of HRMA to detect mutations

HRMA sensitively detects polymorphisms. To minimize detection of polymorphisms present in the backgrounds of WT fish, we typically amplify only a small region of the genome bordering the TALEN target site and analyze that for newly induced mutations. As a result of using small amplicons, we will be unable to detect some TALEN-induced mutations that delete primer-binding sites. As we only rarely observed deletions of >30 bp in the present studies, we believe the majority of TALEN-induced mutations can be identified with the methods described here. It is also possible to detect induced mutations by HRMA using larger amplicons. Finally, although the HRMA studies presented here were performed with a LightScanner (Idaho Technology), we have obtained identical results with similar sensitivity using an Eco Real-Time PCR System (Illumina). Additional instruments initially designed for qPCR analysis are capable of performing HRMA and have been used successfully to detect TALEN-induced mutations (Tatjana Piotrowski and Steven Leach, personal communication).

Ethics statement

All experiments were performed in accordance with, and under the supervision of, the Institutional Animal Care and Use Committee (IACUC) of the University of Utah, which is fully accredited by the AAALAC.

Embryo culture and zebrafish stock maintenance

Wild type zebrafish Danio rerio were of the Tuebingen strain. Zebrafish were maintained under standard conditions and embryos were generated, cultured and staged as described [38], [39].

TALEN target site design

Exon sequences identified from the Zv9 Zebrafish Genome Assembly were scanned for potential TALEN target sites, which were identified using the TALEN Targeter program at https://boglab.plp.iastate.edu/node/add/talen. The following parameters were used: 1) spacer length: 14–17; 2) repeat array length of 16–21; 3) apply all additional options that restrict target choice. Preference was given to target sites: 1) close to the 5′ terminus of the gene to maximize chances of inducing premature translation stop mutations and 2) not in the first exon in case alternative promoters exist. Target sequences that are unique in the genome should be chosen following BLAST analysis to determine that highly similar Left and Right binding sites in close proximity did not exist at other sites in the genome (see Figure 6).

Creation of new backbone vectors

New final backbone vectors used to construct and express genes encoding Left and Right TALEN monomer components were generated here. The new backbone plasmids, pCS2TAL3DD and pCS2TAL3RR, were modified from pCS2-Flag-TTGZFP-FokI-DD and pCS2-HA-GAAZFP-FokI-RR plasmids [23]. First, to render the plasmids suitable for Golden Gate cloning, the single Esp3I restriction enzyme site (in the FokI nuclease domain) of each plasmid was changed from GAGACG to GCGCCG, a mutation that did not alter coding. Second, sequences encoding the ZFP domain were removed following KpnI and BamHI digestion, leaving a backbone vector with sequences 5′ to the KpnI site that provided a 5′UTR, start codon, NLS, and Flag or HA tags and sequences 3′ to the BamHI site that provided a heterodimeric FokI domain, translation termination codon, and SV40 polyA signal. Third, sequences derived from the tal1c gene and ready to accept an RVD repeat array by Golden Gate cloning were placed in frame at the KpnI and BamHI sites. The tal1c sequences were obtained from pTAL3 (sequence positions 1214–2210, www.addgene.org) using primers that added a KpnI site at the 5′ end (TAL3N153F-GTAGGATCCGGTACCGTGGATCTACGCACGCTCGG) and a BamHI site at the 3′ end (TAL3C63R-GTGGGATCCGGCAACGCGATGGGACG) of the amplified pTAL3 sequence. The amplified sequence encoded only a central portion of TAL1c, in which lacZ sequence had been substituted for the RVD repeat array. Cloning into the KpnI/BamHI sites of the backbone vector transferred sequence that provided 136 aa of TALE immediately N′-terminal to the RVD repeat array, an Esp3I restriction enzyme site, lacZ sequences, an Esp3I restriction enzyme site, and 63 aa of TALE immediately C′-terminal to the RVD repeat array. The TALE backbone truncations were designed after TALENs that previously had been shown to function well [27]. Golden Gate cloning of RVD repeat arrays into pCS2TAL3DD or pCS2TAL3RR results in replacement of the lacZ sequences with sequences encoding a designed RVD repeat array and yields a gene encoding an intact TALEN monomer. The pCS2TAL3-DD and pCS2TAL3-RR plasmids are available through Addgene (#37275 and #37276, respectively) with complete sequence information accessible at GenBank (accession numbers JX051360 and JX051361, respectively).

TALEN assembly

The TALEN Golden Gate assembly system described in Cermark et al [30] was used with modifications. RVD repeat arrays were assembled exactly as described [30]. Plasmids providing RVD repeats for Golden Gate cloning are described in [30] and are available through Addgene. Briefly, two rounds of Golden Gate cloning assembly were used to generate a TALEN gene with n RVD repeat modules. First, two arrays were generated, corresponding to repeat modules 1–10 and 11 – n-1. Resulting vectors that acquired arrays were identified as white transformants on IPTG/X-gal plates. Correct assembly was determined first by the size of repeat array inserts liberated following XbaI and AflII restriction enzyme digestion and then sequencing of plasmids with the correct insert size. Second, the two arrays and sequences encoding the n^th motif were transferred into the backbone vectors. RVD repeat array sequences were cloned into pCS2TAL3DD to generate a Left TALEN gene and into pCS2TAL3RR to generate a right TALEN gene. Backbone vectors that acquired arrays were identified as white transformants on IPTG/X-gal plates. Correct assembly was determined first by the size of the insert liberated by SphI and BamHI restriction enzyme digestion and second by sequencing junction regions.

Injection of TALEN RNA into zebrafish embryos

5′-capped mRNA was generated by transcription in vitro of pCS2TAL3DD and pCS2TAL3RR TALEN plasmid templates that had been linearized with NotI (mMESSAGE mMACHINE SP6 kit, Ambion/Invitrogen). Equal amounts of Left and Right TALEN mRNA were injected together into the cytoplasm of 1 cell stage zebrafish embryos.

Genomic DNA extraction

To prepare genomic DNA from embryos, individual 1 or 2 dpf embryos were incubated in 50 ul DNA extraction buffer [10 mM Tris-HCl (pH 8.0), 1 mM EDTA, 50 mM KCl, 0.3% Tween-20, 0.3% NP-40] containing 500 ug/ml proteinase K at 55°C, 2 h. The reaction was terminated by incubation at 99°C, 5 min. The average gDNA concentration was roughly 60 ng/ul.

High Resolution Melt Analysis (HRMA)

To detect TALEN-induced mutations by HRMA, a 90–120 bp amplicon that included the entire genomic target site was generated. Primers flanking the target site were used to amplify the genomic region in a 10 ul PCR reaction containing: 1 ul embryonic gDNA, 1X LightScanner Master Mix (containing the LC Green Plus dye, Idaho Technology), 200 uM each dNTP, and 200 nM each Forward and Reverse primers (see Table S1). Amplification/duplex formation conditions were: denaturation at 95°C, 3 min; 50 cycles {95°C, 30 s–70°C, 18 s}; denaturation at 95°C, 30 s; renaturation at 25°C, 30 s; 10°C. HRMA data was collected on a LightScanner (Idaho Technology) and analyzed using the LightScanner Call-IT Software.