FIG. 9.
Characterization of COPI vesicles immunopurified with antibodies against HA-tagged γ2-COP. Western blot analyses (7.5% acrylamide) of vesicles immunoisolated with an anti-HA antibody from a vesicle preparation generated with cytosol from a HA-tagged γ2-COP-expressing cell line (lane 1) and of various mass amounts of the vesicle preparation, as indicated at the top of lanes 2, 3, and 4, are shown. β′-COP and γ1-COP were stained with an antibody recognizing both proteins (883, upper panel), and γ2-COP was stained with the HA-specific antibody (lower panel).