FIGURE 3.
The decrease of binding rate impaired the cytotoxicity of NK92 cells against K562 cells. A, NK92 and CFSE-labeled K562 were mixed and incubated in 37 °C for different times with or without the presence of 1% EDTA. Then the cells were harvested, fixed, stained with PE-CD56 mAb, and analyzed by flow cytometry. One representative example is shown from three independent experiments. B, cytotoxicity of NK92 cells against K562 cells with or without the presence of 1% EDTA was detected by 4-h 51Cr-release assay. Data were collected from three independent experiments and analyzed by Student's t test, **, p < 0.01.