Binding rate of NK92-target cells was associated with NK92 natural cytotoxicity. A, cytotoxicity of NK92 cells against K562 or HL60 was detected by a 4-h 51Cr-release assay. B, NK92- and CFSE-labeled K562 or HL60 were mixed and incubated in 37 °C for different times. Then the cells were harvested, fixed, stained with PE-CD56 mAb, and analyzed by flow cytometry. Data were collected from at least three independent experiments and analyzed by Student's t test; *, p < 0.05; **, p < 0.01. C, NK92, K562, and HL60 cells were stained with the indicated mAb and analyzed by flow cytometry.